BAG1 Antibody (YA5299)
(Synonyms: Bag 1; BAG family molecular chaperone regulator 1; BAG-1; BAG1; BAG1_HUMAN; BCL 2 Associated Athanogene 1; BCL 2 associated athanogene; BCL 2 binding athanogene 1; Bcl-2-associated athanogene 1; BCL2 associated athanogene 1; BCL2 associated athanogene; Glucocorticoid receptor-associated protein RAP46; HAP1; Haptoglobin; RAP 46; RAP46.)BAG1 Antibody (YA5299) is a Mouse-derived and non-conjugated monoclonal antibody, targeting to BAG1.
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Host:
Mouse
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Application:
WB
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Reactivity :
Human, Mouse
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Formulation:
Supplied in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
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Conjugation:
Non-conjugated
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
Applications
| Application |
WB
WB: Western Blot
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|---|---|
| Dilution Ratio | 1:1000 |
Product Details
BAG1 Antibody (YA5299) is a Mouse-derived and non-conjugated monoclonal antibody, targeting to BAG1.
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Host Mouse
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Clonality Monoclonal
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Species ReactivityHuman, Mouse Predicted Reactivity: Rat
Note: The predicted reactivity is for reference only and should not be considered a guarantee of product performance.
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Observed Molecular WeightObserved band size: 52,46，33 kDa
Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.
Purified recombinant human Bag1 protein fragments expressed in E.coli.
Transfected
affinity chromatography.
Non-conjugated
Unmodified
Product Properties
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Appearance
Liquid
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Formulation
Supplied in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
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Storage & Stability
Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.
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Shipping
Shipping with blue ice.
Background
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Function
BAG1 is a Co-chaperone for HSP70 and HSC70 chaperone proteins. Acts as a nucleotide-exchange factor (NEF) promoting the release of ADP from the HSP70 and HSC70 proteins thereby triggering client/substrate protein release. Nucleotide release is mediated via its binding to the nucleotide-binding domain (NBD) of HSPA8/HSC70 where as the substrate release is mediated via its binding to the substrate-binding domain (SBD) of HSPA8/HSC70. Inhibits the pro-apoptotic function of PPP1R15A, and has anti-apoptotic activity. Markedly increases the anti-cell death function of BCL2 induced by various stimuli. Involved in the STUB1-mediated proteasomal degradation of ESR1 in response to age-related circulating estradiol (17-beta-estradiol/E2) decline, thereby promotes neuronal apoptosis in response to ischemic reperfusion injury[1][2][3][4][5].
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Subcellular Localization
Nucleus; Cytoplasm; Cytoplasm; Nucleus; Cytoplasm; Nucleus
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Expression
Tissue_specificity:Isomer 4 is the most abundant isomer. It is universally expressed in most tissues except the liver, colon, breast, and myometrium. Isomer 1 is expressed in the ovary and testis. Isomer 4 is expressed in various tumor cell lines and is persistently highly expressed in leukemia and lymphoma cell lines. Isomer 1 is expressed in prostate cancer, breast cancer, and leukemia cell lines. Isomer 3 is the least abundant isomer (at the protein level) in tumor cell lines.
Induction:Up-regulated during differentiation of bladder epithelial cells and down-regulated during differentiation of prostate epithelium -
Isoforms & Post-Translational Modification
Q99933 has 4 isomers: Q99933-1: 38779 Da (predicted); Q99933-2: 34866 Da (predicted); Q99933-3: 31117 Da (predicted); Q99933-4: 25989 Da (predicted).
Ubiquitinated; mediated by SIAH1 or SIAH2 and leading to its subsequent proteasomal degradation -
Subunit
Homodimer. Forms a heteromeric complex with HSP70/HSC70 (PubMed:9305631). Binds to the ATPase domain of HSP/HSC70 chaperones. Isoform 1, isoform 3 and isoform 4 but not isoform 2 interact with HSPA8/HSC70 (PubMed:24318877, PubMed:27474739, PubMed:9305631, PubMed:9679980). Interacts with NR3C1 (PubMed:10477749). Interacts with the N-terminal region of MAPRE2 (PubMed:15986447). Interacts with PPP1R15A (PubMed:12724406). Interacts with BCL2 in an ATP-dependent manner. Isoform 2 does not interact with BCL2 (PubMed:9305631). Interacts with SIAH1 (PubMed:9582267). Interacts with HSPA8 (via NBD) (PubMed:24318877, PubMed:27474739). Interacts with HSPA1A (via NBD) and HSPA1B (via NBD) (PubMed:24318877). Interacts with SIAH2 (By similarity). Interacts with ESR1; the interaction is promoted in the absence of estradiol (17-beta-estradiol/E2) (By similarity)
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SwissProt ID
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Synonyms
Bag 1; BAG family molecular chaperone regulator 1; BAG-1; BAG1; BAG1_HUMAN; BCL 2 Associated Athanogene 1; BCL 2 associated athanogene; BCL 2 binding athanogene 1; Bcl-2-associated athanogene 1; BCL2 associated athanogene 1; BCL2 associated athanogene; Glucocorticoid receptor-associated protein RAP46; HAP1; Haptoglobin; RAP 46; RAP46.
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