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ABCG5 Antibody (YA4488)

(Synonyms: STSL)
Customer Review

Based on 1 Customer Validation

ABCG5 Antibody (YA4488) is a Mouse-derived and non-conjugated IgG1 monoclonal antibody, targeting to ABCG5.

For research use only. We do not sell to patients.
  • Host:

    Mouse

  • Isotype:

    IgG

  • Application:

    WB, IHC-P, FC, ELISA

  • Reactivity :

    Human

  • Formulation:

    Supplied in PBS with 0.05% sodium azide

  • Conjugation:
    Non-conjugated
ALL WB FC
  • Experimental Validation Results for ABCG5 Antibody (YA4488)
  • Experimental Validation Results for ABCG5 Antibody (YA4488)
  • Experimental Validation Results for ABCG5 Antibody (YA4488)
  • ABCG5 Antibody (YA4488) Product Image
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To place orders, for customer services and technical support, please contact: MedChemExpress USA

Tel: 609-228-6898 E-mail: [email protected] [email protected]

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Use of MCE Products

Applications

Application
WB Info
WB: Western Blot
IHC-P Info
IHC-P: Immunohistochemistry-Paraffin
FC Info
FC: Flow Cytometry
ELISA Info
ELISA: Enzyme Linked Immunosorbent Assay
Dilution Ratio 1:500-1:2000 1:200-1:1000 1:200-1:400 1:10000

Product Details

Description

ABCG5 Antibody (YA4488) is a Mouse-derived and non-conjugated IgG1 monoclonal antibody, targeting to ABCG5.

  • Host Mouse
  • Clonality Monoclonal
  • Species Reactivity
    Human
  • Observed Molecular Weight
    Observed band size: 32 kDa Info
    Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.
  • Calculated Molecular Weight Predicted band size: 72.5 kDa
Immunogen

Purified recombinant fragment of human ABCG5 (AA: 306-367) expressed in E. Coli.

Purification

affinity purified.

Conjugation

Non-conjugated

Modification

Unmodified

Isotype

IgG

Product Properties

  • Appearance

    Solution

  • Formulation

    Supplied in PBS with 0.05% sodium azide

  • Concentration

    Batch-dependent, Please check the COA for the concentration of each lot. Check Lot Concentration

  • Storage & Stability

    Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

  • Shipping

    Shipping with blue ice.

Verification Images

  • Experimental Validation Results for ABCG5 Antibody (YA4488)
    Western blot analysis of extracts from Jurkat (lane 2(20μg), Jurkat (lane 3(40μg), using ABCG5 Antibody. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in TBST for 2 hour at room temperature. The primary antibody and Loading control antibody (Beta Actin, HY-P80438, 1/3000) was used in 5% BSA in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (HY-P8004/HY-P8001, 1/10,000) was used for 1 hour at room temperature.
  • Experimental Validation Results for ABCG5 Antibody (YA4488)
    Western blot analysis of extracts from HL7702(lane 1(40μg) ) using ABCG5 antibody. Proteins were transferred to a NC membrane and blocked with 5% Skim milk in TBST for 2 hour at room temperature. The primary antibody ( 1/1000) and Loading control antibody (GAPDH，1/1000) was used in 5% Skim milk in TBST at 4°C overnight. Goat Anti-Mouse IgG-HRP Secondary Antibody (1/10,000) was used for 1 hour at room temperature.
  • Experimental Validation Results for ABCG5 Antibody (YA4488)
    Flow cytometric analysis of 1X10^6 A549 cells labeling ABCG5 Antibody(red). Cells were fixed with 4% paraformaldehyde and permeabilised with 0.2% Triton X-100. Then stained with the primary antibody at 1/400 dilution overnight at 4℃.Alexa Fluor® 488 Goat Anti-mouse IgG H&L (invitrogen A11001) was used as the secondary antibody at 1/1,000 dilution for 45 minutes at room temperature. Mouse IgG Isotype Control (Invitrogen 14-4714-B2, blue) was used as the isotype control, cells without incubation with primary antibody were used as the unlabeled control (gray).

Background

  • Function

    ABCG5 is an ABCG5 and ABCG8 form an obligate heterodimer that mediates Mg(2+)- and ATP-dependent sterol transport across the cell membrane. Plays an essential role in the selective transport of dietary plant sterols and cholesterol in and out of the enterocytes and in the selective sterol excretion by the liver into bile. Required for normal sterol homeostasis. The heterodimer with ABCG8 has ATPase activity[1][2][3][4][5][6].

  • Subcellular Localization

    Cell membrane; Multi-pass membrane protein; Apical cell membrane; Multi-pass membrane protein

  • Expression


    Tissue_specificity:Strongly expressed in the liver, lower levels in the small intestine and colon

  • Subunit

    Heterodimer with ABCG8

  • SwissProt ID

    Q9H222

  • Gene ID
    64240 [NCBI]
  • Synonyms

    STSL

ABCG5 Antibody (YA4488) Related Classifications

MOQ
Minimum order quantity
100 mg

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Measured by its ability to inhibit anti-CD3-induced proliferation of stimulated CTLL-2 mouse cytotoxic T cells. The ED50 this effect is 0.8023 μg/ml, corresponding to a specific activity is 1246.42 units/mg.

Assay Types
  • 1/16

    MedchemExpress Validation
     Western blot analysis of extracts from Jurkat (lane 2(20μg), Jurkat (lane 3(40μg), using ABCG5 Antibody. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in TBST for 2 hour at room temperature. The primary antibody and Loading control antibody (Beta Actin, HY-P80438, 1/3000) was used in 5% BSA in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (HY-P8004/HY-P8001, 1/10,000) was used for 1 hour at room temperature.

  • 1/16

    MedChemExpress Validation
     Western blot analysis of extracts from HL7702(lane 1(40μg) ) using ABCG5 antibody. Proteins were transferred to a NC membrane and blocked with 5% Skim milk in TBST for 2 hour at room temperature. The primary antibody ( 1/1000) and Loading control antibody (GAPDH，1/1000) was used in 5% Skim milk in TBST at 4°C overnight. Goat Anti-Mouse IgG-HRP Secondary Antibody (1/10,000) was used for 1 hour at room temperature.

  • 1/16

    MedChemExpress Validation
     Flow cytometric analysis of 1X10^6 A549 cells labeling ABCG5 Antibody(red). Cells were fixed with 4% paraformaldehyde and permeabilised with 0.2% Triton X-100. Then stained with the primary antibody at 1/400 dilution overnight at 4℃.Alexa Fluor® 488 Goat Anti-mouse IgG H&L (invitrogen A11001) was used as the secondary antibody at 1/1,000 dilution for 45 minutes at room temperature. Mouse IgG Isotype Control (Invitrogen 14-4714-B2, blue) was used as the isotype control, cells without incubation with primary antibody were used as the unlabeled control (gray).

  • ABCG5 Antibody (YA4488) Product Image
    1/16
  • Experimental Validation Results for ABCG5 Antibody (YA4488)
  • Experimental Validation Results for ABCG5 Antibody (YA4488)
  • Experimental Validation Results for ABCG5 Antibody (YA4488)
  • ABCG5 Antibody (YA4488) Product Image
  • Experimental Validation Results for ABCG5 Antibody (YA4488)
    1/1
    MedchemExpress Validation
     Western blot analysis of extracts from Jurkat (lane 2(20μg), Jurkat (lane 3(40μg), using ABCG5 Antibody. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in TBST for 2 hour at room temperature. The primary antibody and Loading control antibody (Beta Actin, HY-P80438, 1/3000) was used in 5% BSA in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (HY-P8004/HY-P8001, 1/10,000) was used for 1 hour at room temperature.
  • Experimental Validation Results for ABCG5 Antibody (YA4488)
    1/1
    MedChemExpress Validation
     Western blot analysis of extracts from HL7702(lane 1(40μg) ) using ABCG5 antibody. Proteins were transferred to a NC membrane and blocked with 5% Skim milk in TBST for 2 hour at room temperature. The primary antibody ( 1/1000) and Loading control antibody (GAPDH，1/1000) was used in 5% Skim milk in TBST at 4°C overnight. Goat Anti-Mouse IgG-HRP Secondary Antibody (1/10,000) was used for 1 hour at room temperature.
  • Experimental Validation Results for ABCG5 Antibody (YA4488)
    1/1
    MedChemExpress Validation
     Flow cytometric analysis of 1X10^6 A549 cells labeling ABCG5 Antibody(red). Cells were fixed with 4% paraformaldehyde and permeabilised with 0.2% Triton X-100. Then stained with the primary antibody at 1/400 dilution overnight at 4℃.Alexa Fluor® 488 Goat Anti-mouse IgG H&L (invitrogen A11001) was used as the secondary antibody at 1/1,000 dilution for 45 minutes at room temperature. Mouse IgG Isotype Control (Invitrogen 14-4714-B2, blue) was used as the isotype control, cells without incubation with primary antibody were used as the unlabeled control (gray).
  • Experimental Validation Results for ABCG5 Antibody (YA4488)
  • Experimental Validation Results for ABCG5 Antibody (YA4488)
  • Experimental Validation Results for ABCG5 Antibody (YA4488)
  • ABCG5 Antibody (YA4488) Product Image
    MedchemExpress Validation

    Western blot analysis of extracts from Jurkat (lane 2(20μg), Jurkat (lane 3(40μg), using ABCG5 Antibody. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in TBST for 2 hour at room temperature. The primary antibody and Loading control antibody (Beta Actin, HY-P80438, 1/3000) was used in 5% BSA in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (HY-P8004/HY-P8001, 1/10,000) was used for 1 hour at room temperature.

  • ABCG5 Antibody (YA4488) Product Image
    MedChemExpress Validation

    Western blot analysis of extracts from HL7702(lane 1(40μg) ) using ABCG5 antibody. Proteins were transferred to a NC membrane and blocked with 5% Skim milk in TBST for 2 hour at room temperature. The primary antibody ( 1/1000) and Loading control antibody (GAPDH，1/1000) was used in 5% Skim milk in TBST at 4°C overnight. Goat Anti-Mouse IgG-HRP Secondary Antibody (1/10,000) was used for 1 hour at room temperature.

  • ABCG5 Antibody (YA4488) Product Image
    MedChemExpress Validation

    Flow cytometric analysis of 1X10^6 A549 cells labeling ABCG5 Antibody(red). Cells were fixed with 4% paraformaldehyde and permeabilised with 0.2% Triton X-100. Then stained with the primary antibody at 1/400 dilution overnight at 4℃.Alexa Fluor® 488 Goat Anti-mouse IgG H&L (invitrogen A11001) was used as the secondary antibody at 1/1,000 dilution for 45 minutes at room temperature. Mouse IgG Isotype Control (Invitrogen 14-4714-B2, blue) was used as the isotype control, cells without incubation with primary antibody were used as the unlabeled control (gray).

  • ABCG5 Antibody (YA4488) Product Image WB
  • ABCG5 Antibody (YA4488) Product Image WB
  • ABCG5 Antibody (YA4488) Product Image FC