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Activin Receptor Type IIB Antibody (YA2345)

(Synonyms: ActR IIB; ACVR 2B; HTX4)
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Based on 1 Customer Validation

Activin Receptor Type IIB Antibody (YA2345) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to Activin Receptor Type IIB.

For research use only. We do not sell to patients.
  • Host:

    Rabbit

  • Isotype:

    IgG

  • Application:

    WB

  • Reactivity :

    Human, Mouse, Rat

  • Formulation:

    Supplied in 50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40%Glycerol, 0.01% sodium azide and 0.05% BSA.

  • Conjugation:
    Non-conjugated
  • Experimental Validation Results for Activin Receptor Type IIB Antibody (YA2345)
  • Activin Receptor Type IIB Antibody (YA2345) Product Image
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Tel: 609-228-6898 E-mail: [email protected] [email protected]

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Applications

Application
WB Info
WB: Western Blot
Dilution Ratio 1:500-1:1000

Product Details

Description

Activin Receptor Type IIB Antibody (YA2345) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to Activin Receptor Type IIB.

  • Host Rabbit
  • Clonality Recombinant,Monoclonal
  • Species Reactivity
    Human, Mouse, Rat
  • Observed Molecular Weight
    Observed band size: 65 kDa Info
    Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.
  • Calculated Molecular Weight Predicted band size: 58 kDa
Species Reactivity Database
Immunogen

A synthesized peptide derived from human Activin Receptor Type IIB aa150-200 aa150-200/512.

Sensitivity

Endogenous

Purification

Affinity Purified

Conjugation

Non-conjugated

Modification

Unmodified

Isotype

IgG

RRID

AB_3104701

Product Properties

  • Appearance

    Liquid

  • Formulation

    Supplied in 50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40%Glycerol, 0.01% sodium azide and 0.05% BSA.

  • Concentration

    Batch-dependent, Please check the COA for the concentration of each lot. Check Lot Concentration

  • Storage & Stability

    Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

  • Shipping

    Shipping with blue ice.

Verification Images

  • Experimental Validation Results for Activin Receptor Type IIB Antibody (YA2345)
    Western blot analysis was performed on extracts from 293T (lane 1, 15 μg), K562 (lane 2, 15 μg), Hela (lane 3, 15 μg), Jurkat (lane 4, 15 μg), and 293 (lane 5, 15 μg) using Activin Receptor Type IIB Rabbit mAb.Proteins were transferred to a PVDF membrane and blocked with 5% non - fat milk in TBST at 4°C overnight.The primary antibody (1:1000 dilution) and the loading control antibody (GAPDH, HY-P80137, 1:60000 dilution) were incubated in 5% non-fat milk in TBST for 1 hour at 37°C.Goat Anti - Rabbit IgG - HRP Secondary Antibody (1:20000 dilution) was then applied for 40 minutes at 37°C.

Background

  • Function

    Activin Receptor Type IIB is a Transmembrane serine/threonine kinase activin type-2 receptor forming an activin receptor complex with activin type-1 serine/threonine kinase receptors (ACVR1, ACVR1B or ACVR1c). Transduces the activin signal from the cell surface to the cytoplasm and is thus regulating many physiological and pathological processes including neuronal differentiation and neuronal survival, hair follicle development and cycling, FSH production by the pituitary gland, wound healing, extracellular matrix production, immunosuppression and carcinogenesis. Activin is also thought to have a paracrine or autocrine role in follicular development in the ovary. Within the receptor complex, the type-2 receptors act as a primary activin receptors (binds activin-A/INHBA, activin-B/INHBB as well as inhibin-A/INHA-INHBA). The type-1 receptors like ACVR1B act as downstream transducers of activin signals. Activin binds to type-2 receptor at the plasma membrane and activates its serine-threonine kinase. The activated receptor type-2 then phosphorylates and activates the type-1 receptor. Once activated, the type-1 receptor binds and phosphorylates the SMAD proteins SMAD2 and SMAD3, on serine residues of the C-terminal tail. Soon after their association with the activin receptor and subsequent phosphorylation, SMAD2 and SMAD3 are released into the cytoplasm where they interact with the common partner SMAD4. This SMAD complex translocates into the nucleus where it mediates activin-induced transcription. Inhibitory SMAD7, which is recruited to ACVR1B through FKBP1A, can prevent the association of SMAD2 and SMAD3 with the activin receptor complex, thereby blocking the activin signal. Activin signal transduction is also antagonized by the binding to the receptor of inhibin-B via the IGSF1 inhibin coreceptor

  • Subcellular Localization

    Cell membrane; Single-pass type I membrane protein

  • Isoforms & Post-Translational Modification

    Q13705 has 2 isomers: Q13705-1: 57724 Da (predicted); Q13705-2: Da (predicted).
    Phosphorylated. Constitutive phosphorylation is in part catalyzed by its own kinase activity

  • Subunit

    Forms an activin receptor complex with activin type II receptors such as ACVR1B. Interacts with VPS39. Interacts with DYNLT1. Interacts with BMP3 (PubMed:31665064). Interacts with BMP2 (PubMed:17295905). Interacts with BMP6 (PubMed:18070108)

  • SwissProt ID

    Q13705

  • Gene ID
    93 [NCBI]
  • Synonyms

    ActR IIB; ACVR 2B; HTX4

  • Research Field

    Signal Transduction

Activin Receptor Type IIB Antibody (YA2345) Related Classifications

MOQ
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100 mg

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Measured by its ability to inhibit anti-CD3-induced proliferation of stimulated CTLL-2 mouse cytotoxic T cells. The ED50 this effect is 0.8023 μg/ml, corresponding to a specific activity is 1246.42 units/mg.

Assay Types
  • 1/16

    MedchemExpress Validation
     Western blot analysis was performed on extracts from 293T (lane 1, 15 μg), K562 (lane 2, 15 μg), Hela (lane 3, 15 μg), Jurkat (lane 4, 15 μg), and 293 (lane 5, 15 μg) using Activin Receptor Type IIB Rabbit mAb.Proteins were transferred to a PVDF membrane and blocked with 5% non - fat milk in TBST at 4°C overnight.The primary antibody (1:1000 dilution) and the loading control antibody (GAPDH, HY-P80137, 1:60000 dilution) were incubated in 5% non-fat milk in TBST for 1 hour at 37°C.Goat Anti - Rabbit IgG - HRP Secondary Antibody (1:20000 dilution) was then applied for 40 minutes at 37°C.

  • Activin Receptor Type IIB Antibody (YA2345) Product Image
    1/16
  • Experimental Validation Results for Activin Receptor Type IIB Antibody (YA2345)
  • Activin Receptor Type IIB Antibody (YA2345) Product Image
  • Experimental Validation Results for Activin Receptor Type IIB Antibody (YA2345)
    1/1
    MedchemExpress Validation
     Western blot analysis was performed on extracts from 293T (lane 1, 15 μg), K562 (lane 2, 15 μg), Hela (lane 3, 15 μg), Jurkat (lane 4, 15 μg), and 293 (lane 5, 15 μg) using Activin Receptor Type IIB Rabbit mAb.Proteins were transferred to a PVDF membrane and blocked with 5% non - fat milk in TBST at 4°C overnight.The primary antibody (1:1000 dilution) and the loading control antibody (GAPDH, HY-P80137, 1:60000 dilution) were incubated in 5% non-fat milk in TBST for 1 hour at 37°C.Goat Anti - Rabbit IgG - HRP Secondary Antibody (1:20000 dilution) was then applied for 40 minutes at 37°C.
  • Experimental Validation Results for Activin Receptor Type IIB Antibody (YA2345)
  • Activin Receptor Type IIB Antibody (YA2345) Product Image
    MedchemExpress Validation

    Western blot analysis was performed on extracts from 293T (lane 1, 15 μg), K562 (lane 2, 15 μg), Hela (lane 3, 15 μg), Jurkat (lane 4, 15 μg), and 293 (lane 5, 15 μg) using Activin Receptor Type IIB Rabbit mAb.Proteins were transferred to a PVDF membrane and blocked with 5% non - fat milk in TBST at 4°C overnight.The primary antibody (1:1000 dilution) and the loading control antibody (GAPDH, HY-P80137, 1:60000 dilution) were incubated in 5% non-fat milk in TBST for 1 hour at 37°C.Goat Anti - Rabbit IgG - HRP Secondary Antibody (1:20000 dilution) was then applied for 40 minutes at 37°C.

  • Activin Receptor Type IIB Antibody (YA2345) Product Image WB