ERCC1 Antibody (YA5484)

Cat. No.: HY-P85792: Data Sheet User Guide for Antibodies
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ERCC1 Antibody (YA5484) is a Mouse-derived and non-conjugated IgG1 monoclonal antibody, targeting to ERCC1.

For research use only. We do not sell to patients.

Size	Stock	Quantity
20 μL	Get quote 2 - 3 Weeks 1 - 2 Weeks 3 - 4 Weeks 2 - 3 weeks	Estimated Time of Arrival: December 31
50 μL	Get quote 2 - 3 Weeks 1 - 2 Weeks 3 - 4 Weeks 2 - 3 weeks	Estimated Time of Arrival: December 31
100 μL	Get quote 2 - 3 Weeks 1 - 2 Weeks 3 - 4 Weeks 2 - 3 weeks	Estimated Time of Arrival: December 31
250 μL	Get quote

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WB: Western Blot;
IHC-P: Immunohistochemistry-Paraffin;
IHC-F: Immunohistochemistry-Frozen;
ICC/IF: Immunocytochemistry/Immunofluorescence;
IF-Tissue: Immunofluorescence-Tissue;
mIHC: Multiplex Immunohistochemical;
IP: Immunoprecipitation;
ChIP: Chromatin Immunoprecipitation;
FC: Flow Cytometry;
ELISA: Enzyme Linked Immunosorbent Assay

Product Detail
Background
Documentation

Description

ERCC1 Antibody (YA5484) is a Mouse-derived and non-conjugated IgG1 monoclonal antibody, targeting to ERCC1.

Host

Mouse

Clonality

Monoclonal

Molecular Weight

Predicted band size: 33 kDa;

Observed band size: 33 kDa

Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.

Species Reactivity

Human

SwissProt ID

P07992

Gene ID

2067 [NCBI]

Immunogen

Synthesized peptide derived from human ERCC1 AA range: 1-100

Application &
Dilution Ratio

Application	Dilution Ratio
WB WB: Western Blot	1:500-2000
ICC/IF ICC/IF: Immunocytochemistry/Immunofluorescence	1:100-500
ELISA ELISA: Enzyme Linked Immunosorbent Assay	1:1000-5000

Purity	Protein G	Conjugation	Non-conjugated
Modification	Unmodified	Isotype	IgG1

Appearance

Liquid

Formulation

Supplied in PBS, 50% glycerol, 0.05% Proclin 300, 0.05%BSA

Storage & Stability

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

Shipping

Shipping with blue ice.

Background

Function：Non-catalytic component of a structure-specific DNA repair endonuclease responsible for the 5'-incision during DNA repair. Responsible, in conjunction with SLX4, for the first step in the repair of interstrand cross-links (ICL). Participates in the processing of anaphase bridge-generating DNA structures, which consist in incompletely processed DNA lesions arising during S or G2 phase, and can result in cytokinesis failure. Also required for homology-directed repair (HDR) of DNA double-strand breaks, in conjunction with SLX4; Not functional in the nucleotide excision repair pathway; Not functional in the nucleotide excision repair pathway; Not functional in the nucleotide excision repair pathway

Subcellular Localization：Nucleus; Cytoplasm; Nucleus; Nucleus; Nucleus

Isoforms & Post-Translational Modification：P07992 has 4 isomers: P07992-1: 32562 Da (predicted); P07992-2: 29993 Da (predicted); P07992-3: 35563 Da (predicted); P07992-4: 25211 Da (predicted).
Ubiquitinated with both 'Lys-48' and 'Lys-63' linkages (PubMed:25538220). Deubiquitinated by USP45 (PubMed:25538220)

Subunit：Heterodimer composed of ERCC1 isoform 1 and ERCC4/XPF (PubMed:16076955, PubMed:16338413, PubMed:24036546, PubMed:25538220, PubMed:32034146). Interacts with USP45 (PubMed:25538220)

Synonyms

DNA excision repair protein ERCC-1

Documentation

User Guide for Antibodies (1077 KB)