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CRMP1 Antibody (YA1941)

(Synonyms: CRMP 1; Crmp1; DPYSL1; DRP1; ULIP-3; Ulip3)
Customer Review

Based on 1 Customer Validation

CRMP1 Antibody (YA1941) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to CRMP1.

For research use only. We do not sell to patients.
  • Host:

    Rabbit

  • Isotype:

    IgG

  • Application:

    WB, IHC-P, ICC/IF, FC, IP

  • Reactivity :

    Human, Mouse, Rat

  • Formulation:

    Supplied in rabbit IgG in 50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40%Glycerol, 0.01% sodium azide and 0.05% BSA.

  • Conjugation:
    Non-conjugated
ALL IHC-P FC
  • Experimental Validation Results for CRMP1 Antibody (YA1941)
  • Experimental Validation Results for CRMP1 Antibody (YA1941)
  • Experimental Validation Results for CRMP1 Antibody (YA1941)
  • Experimental Validation Results for CRMP1 Antibody (YA1941)
  • CRMP1 Antibody (YA1941) Product Image
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To place orders, for customer services and technical support, please contact: MedChemExpress USA

Tel: 609-228-6898 E-mail: [email protected] [email protected]

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Data Download
Related Products:
Top Publications Citing

Use of MCE Products

Applications

Application
WB Info
WB: Western Blot
IHC-P Info
IHC-P: Immunohistochemistry-Paraffin
ICC/IF Info
ICC/IF: Immunocytochemistry/
Immunofluorescence
FC Info
FC: Flow Cytometry
IP Info
IP: Immunoprecipitation
Dilution Ratio 1:1000-1:2000 1:100-1:200 1:50-1:200 1:50-1:100 1:40

Product Details

Description

CRMP1 Antibody (YA1941) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to CRMP1.

  • Host Rabbit
  • Clonality Recombinant,Monoclonal
  • Species Reactivity
    Human, Mouse, Rat
  • Observed Molecular Weight
    Observed band size: 64-80 kDa Info
    Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.
  • Calculated Molecular Weight Predicted band size: 62 kDa
Species Reactivity Database
Immunogen

A synthesized peptide derived from human CRMP1 aa500-572.

Sensitivity

Endogenous

Purification

Affinity Chromatography

Conjugation

Non-conjugated

Modification

Unmodified

Isotype

IgG

Product Properties

  • Appearance

    Solution

  • Formulation

    Supplied in rabbit IgG in 50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40%Glycerol, 0.01% sodium azide and 0.05% BSA.

  • Concentration

    Batch-dependent, Please check the COA for the concentration of each lot. Check Lot Concentration

  • Storage & Stability

    Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

  • Shipping

    Shipping with blue ice.

Verification Images

  • Experimental Validation Results for CRMP1 Antibody (YA1941)
    Immunohistochemical analysis of paraffin-embedded mouse cerebral cortex tissue using CRMP1 Antibody (HY-P82196, 1/200). The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
  • Experimental Validation Results for CRMP1 Antibody (YA1941)
    Immunohistochemical analysis of paraffin-embedded mouse hippocampus tissue using CRMP1 Antibody (HY-P82196, 1/200). The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
  • Experimental Validation Results for CRMP1 Antibody (YA1941)
    Immunohistochemical analysis of paraffin-embedded rat cerebral cortex tissue using CRMP1 Antibody (HY-P82196, 1/200). The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
  • Experimental Validation Results for CRMP1 Antibody (YA1941)
    Flow cytometric analysis of 1X106 SH-SY5Y cells labeling CRMP1 Antibody (HY-P82196, red). Cells were fixed with 4% paraformaldehyde and permeabilised with 90% methanol. Then stained with the primary antibody at 1/100 dilution for an hour at 4℃. AF488-conjugated Goat Anti-Rabbit IgG H&L (HY-P8002) was used as the secondary antibody at 1/1,000 dilution for 30 minutes at 4℃. Rabbit IgG Isotype Control (HY-P80879, blue) was used as the isotype control, cells without incubation with primary antibody were used as the unlabeled control (black).

Background

  • Function

    CRMP1 is a Necessary for signaling by class 3 semaphorins and subsequent remodeling of the cytoskeleton. Plays a role in axon guidance. During the axon guidance process, acts downstream of SEMA3A to promote FLNA dissociation from F-actin which results in the rearrangement of the actin cytoskeleton and the collapse of the growth cone. Involved in invasive growth and cell migration. May participate in cytokinesis[1][2][3].

  • Subcellular Localization

    Cytoplasm; Cytoplasm, cytoskeleton, microtubule organizing center, centrosome; Cytoplasm, cytoskeleton, spindle; Cell projection, growth cone; Cytoplasm, cytoskeleton; Perikaryon

  • Expression


    Tissue_specificity:Brain

  • Isoforms & Post-Translational Modification

    Q14194 has 2 isomers: Q14194-1: 62184 Da (predicted); Q14194-2: 74262 Da (predicted).
    Phosphorylation at Ser-522 enhances CRMP1-mediated alteration of FLNA ternary structure and FLNA dissociation from F-actin

  • Subunit

    Homotetramer, and heterotetramer with DPYSL2, DPYSL3, DPYSL4 or DPYSL5 (By similarity). Interacts with PLXNA1 (By similarity). Interacts with FLNA (via calponin-homology (CH) domain 1 and filamin repeat 24); the interaction alters FLNA ternary structure and thus promotes FLNA dissociation from F-actin (PubMed:25358863)

  • SwissProt ID

    Q14194

  • Gene ID
    1400 [NCBI]
  • Synonyms

    CRMP 1; Crmp1; DPYSL1; DRP1; ULIP-3; Ulip3

  • Research Field

    Neuroscience

CRMP1 Antibody (YA1941) Related Classifications

MOQ
Minimum order quantity
100 mg

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Measured by its ability to inhibit anti-CD3-induced proliferation of stimulated CTLL-2 mouse cytotoxic T cells. The ED50 this effect is 0.8023 μg/ml, corresponding to a specific activity is 1246.42 units/mg.

Assay Types
  • 1/16

    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded mouse cerebral cortex tissue using CRMP1 Antibody (HY-P82196, 1/200). The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • 1/16

    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded mouse hippocampus tissue using CRMP1 Antibody (HY-P82196, 1/200). The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • 1/16

    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded rat cerebral cortex tissue using CRMP1 Antibody (HY-P82196, 1/200). The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • 1/16

    MedChemExpress Validation
     Flow cytometric analysis of 1X106 SH-SY5Y cells labeling CRMP1 Antibody (HY-P82196, red). Cells were fixed with 4% paraformaldehyde and permeabilised with 90% methanol. Then stained with the primary antibody at 1/100 dilution for an hour at 4℃. AF488-conjugated Goat Anti-Rabbit IgG H&L (HY-P8002) was used as the secondary antibody at 1/1,000 dilution for 30 minutes at 4℃. Rabbit IgG Isotype Control (HY-P80879, blue) was used as the isotype control, cells without incubation with primary antibody were used as the unlabeled control (black).

  • CRMP1 Antibody (YA1941) Product Image
    1/16
  • Experimental Validation Results for CRMP1 Antibody (YA1941)
  • Experimental Validation Results for CRMP1 Antibody (YA1941)
  • Experimental Validation Results for CRMP1 Antibody (YA1941)
  • Experimental Validation Results for CRMP1 Antibody (YA1941)
  • CRMP1 Antibody (YA1941) Product Image
  • Experimental Validation Results for CRMP1 Antibody (YA1941)
    1/1
    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded mouse cerebral cortex tissue using CRMP1 Antibody (HY-P82196, 1/200). The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
  • Experimental Validation Results for CRMP1 Antibody (YA1941)
    1/1
    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded mouse hippocampus tissue using CRMP1 Antibody (HY-P82196, 1/200). The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
  • Experimental Validation Results for CRMP1 Antibody (YA1941)
    1/1
    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded rat cerebral cortex tissue using CRMP1 Antibody (HY-P82196, 1/200). The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
  • Experimental Validation Results for CRMP1 Antibody (YA1941)
    1/1
    MedChemExpress Validation
     Flow cytometric analysis of 1X106 SH-SY5Y cells labeling CRMP1 Antibody (HY-P82196, red). Cells were fixed with 4% paraformaldehyde and permeabilised with 90% methanol. Then stained with the primary antibody at 1/100 dilution for an hour at 4℃. AF488-conjugated Goat Anti-Rabbit IgG H&L (HY-P8002) was used as the secondary antibody at 1/1,000 dilution for 30 minutes at 4℃. Rabbit IgG Isotype Control (HY-P80879, blue) was used as the isotype control, cells without incubation with primary antibody were used as the unlabeled control (black).
  • Experimental Validation Results for CRMP1 Antibody (YA1941)
  • Experimental Validation Results for CRMP1 Antibody (YA1941)
  • Experimental Validation Results for CRMP1 Antibody (YA1941)
  • Experimental Validation Results for CRMP1 Antibody (YA1941)
  • CRMP1 Antibody (YA1941) Product Image
    MedChemExpress Validation

    Immunohistochemical analysis of paraffin-embedded mouse cerebral cortex tissue using CRMP1 Antibody (HY-P82196, 1/200). The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • CRMP1 Antibody (YA1941) Product Image
    MedChemExpress Validation

    Immunohistochemical analysis of paraffin-embedded mouse hippocampus tissue using CRMP1 Antibody (HY-P82196, 1/200). The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • CRMP1 Antibody (YA1941) Product Image
    MedChemExpress Validation

    Immunohistochemical analysis of paraffin-embedded rat cerebral cortex tissue using CRMP1 Antibody (HY-P82196, 1/200). The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • CRMP1 Antibody (YA1941) Product Image
    MedChemExpress Validation

    Flow cytometric analysis of 1X106 SH-SY5Y cells labeling CRMP1 Antibody (HY-P82196, red). Cells were fixed with 4% paraformaldehyde and permeabilised with 90% methanol. Then stained with the primary antibody at 1/100 dilution for an hour at 4℃. AF488-conjugated Goat Anti-Rabbit IgG H&L (HY-P8002) was used as the secondary antibody at 1/1,000 dilution for 30 minutes at 4℃. Rabbit IgG Isotype Control (HY-P80879, blue) was used as the isotype control, cells without incubation with primary antibody were used as the unlabeled control (black).

  • CRMP1 Antibody (YA1941) Product Image IHC-P
  • CRMP1 Antibody (YA1941) Product Image IHC-P
  • CRMP1 Antibody (YA1941) Product Image IHC-P
  • CRMP1 Antibody (YA1941) Product Image FC