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PAKγ Antibody (YA7384)

PAKγ Antibody (YA7384) is a Rabbit-derived and non-conjugated IgG, Kappa monoclonal antibody, targeting to PAKγ.

For research use only. We do not sell to patients.
  • Host:

    Rabbit

  • Isotype:

    IgG,Kappa

  • Application:

    WB, IHC-P, ICC/IF, ELISA

  • Reactivity :

    Human, Mouse, Rat

  • Formulation:

    Supplied in PBS (pH7.4) containing 50% glycerol, 0.05% Proclin 300, 0.05%BSA

  • Conjugation:
    Non-conjugated
  • Experimental Validation Results for PAKγ Antibody (YA7384)
  • PAKγ Antibody (YA7384) Product Image
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To place orders, for customer services and technical support, please contact: MedChemExpress USA

Tel: 609-228-6898 E-mail: [email protected] [email protected]

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Use of MCE Products

Applications

Application
IHC-P Info
IHC-P: Immunohistochemistry-Paraffin
WB Info
WB: Western Blot
ICC/IF Info
ICC/IF: Immunocytochemistry/
Immunofluorescence
ELISA Info
ELISA: Enzyme Linked Immunosorbent Assay
Dilution Ratio 1:200-1:1000 1:2000-1:10000 1:200-1:1000 1:5000-1:20000

Product Details

Description

PAKγ Antibody (YA7384) is a Rabbit-derived and non-conjugated IgG, Kappa monoclonal antibody, targeting to PAKγ.

  • Host Rabbit
  • Clonality Monoclonal,Recombinant
  • Species Reactivity
    Human, Mouse, Rat
  • Observed Molecular Weight
    Observed band size: 58 kDa Info
    Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.
  • Calculated Molecular Weight Predicted band size: 58 kDa
Immunogen

The exact sequence is proprietary to MCE.

Sensitivity

Endogenous

Purification

Protein A affinity purified

Conjugation

Non-conjugated

Modification

Unmodified

Isotype

IgG,Kappa

Product Properties

  • Appearance

    Liquid

  • Formulation

    Supplied in PBS (pH7.4) containing 50% glycerol, 0.05% Proclin 300, 0.05%BSA

  • Storage & Stability

    Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

  • Shipping

    Shipping with blue ice.

Verification Images

  • Experimental Validation Results for PAKγ Antibody (YA7384)
    Western blot analysis was performed on extracts from Hek293 (lane 1, 20μg), HepG2 (lane 2, 20μg), HCT116 (lane 3, 20μg), A594 (lane 4, 20μg), using PAKγ Antibody (YA7384) . Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST at 25°C for 1hour.The primary antibody (1:2000 dilution) and the loading control antibody (GAPDH, HY-P83944, 1:3000 dilution) were incubated in 5% non-fat milk in TBST for overnight. AF800-conjugated Goat Anti-Rabbit IgG H&L(HY-P810687, 1:1000 dilution) and AF680-conjugated Goat Anti-Mouse IgG H&L(HY-P89186 , 1:10000 dilution) was then applied for 1 hour at 37°C, protect from light.

Background

  • Function

    PAKγ is a Serine/threonine protein kinase that plays a role in a variety of different signaling pathways including cytoskeleton regulation, cell motility, cell cycle progression, apoptosis or proliferation. Acts as a downstream effector of the small GTPases CDC42 and RAC1. Activation by the binding of active CDC42 and RAC1 results in a conformational change and a subsequent autophosphorylation on several serine and/or threonine residues. Full-length PAK2 stimulates cell survival and cell growth. Phosphorylates MAPK4 and MAPK6 and activates the downstream target MAPKAPK5, a regulator of F-actin polymerization and cell migration. Phosphorylates JUN and plays an important role in EGF-induced cell proliferation. Phosphorylates many other substrates including histone H4 to promote assembly of H3.3 and H4 into nucleosomes, BAD, ribosomal protein S6, or MBP. Phosphorylates CASP7, thereby preventing its activity. Additionally, associates with ARHGEF7 and GIT1 to perform kinase-independent functions such as spindle orientation control during mitosis. On the other hand, apoptotic stimuli such as DNA damage lead to caspase-mediated cleavage of PAK2, generating PAK-2p34, an active p34 fragment that translocates to the nucleus and promotes cellular apoptosis involving the JNK signaling pathway. Caspase-activated PAK2 phosphorylates MKNK1 and reduces cellular translation[1][2][3][4][5][6][7][8][9][10][11][12][13].

  • Subcellular Localization

    Cytoplasm,Nucleus,Nucleus,Cytoplasm, perinuclear region,Membrane

  • Expression


    Tissue_Specificity: Ubiquitously expressed. Higher levels seen in skeletal muscle, ovary, thymus and spleen

  • Isoforms & Post-Translational Modification

    Q13177: 524 amino acids, molecular weight 58043 Da.

  • Subunit

    Interacts tightly with GTP-bound but not GDP-bound CDC42/p21 and RAC1 (PubMed:20696164)

  • SwissProt ID

    Q13177

PAKγ Antibody (YA7384) Related Classifications

MOQ
Minimum order quantity
100 mg

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Measured by its ability to inhibit anti-CD3-induced proliferation of stimulated CTLL-2 mouse cytotoxic T cells. The ED50 this effect is 0.8023 μg/ml, corresponding to a specific activity is 1246.42 units/mg.

Assay Types
  • 1/16

    MedChemExpress Validation
     Western blot analysis was performed on extracts from Hek293 (lane 1, 20μg), HepG2 (lane 2, 20μg), HCT116 (lane 3, 20μg), A594 (lane 4, 20μg), using PAKγ Antibody (YA7384) . Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST at 25°C for 1hour.The primary antibody (1:2000 dilution) and the loading control antibody (GAPDH, HY-P83944, 1:3000 dilution) were incubated in 5% non-fat milk in TBST for overnight. AF800-conjugated Goat Anti-Rabbit IgG H&L(HY-P810687, 1:1000 dilution) and AF680-conjugated Goat Anti-Mouse IgG H&L(HY-P89186 , 1:10000 dilution) was then applied for 1 hour at 37°C, protect from light.

  • PAKγ Antibody (YA7384) Product Image
    1/16
  • Experimental Validation Results for PAKγ Antibody (YA7384)
  • PAKγ Antibody (YA7384) Product Image
  • Experimental Validation Results for PAKγ Antibody (YA7384)
    1/1
    MedChemExpress Validation
     Western blot analysis was performed on extracts from Hek293 (lane 1, 20μg), HepG2 (lane 2, 20μg), HCT116 (lane 3, 20μg), A594 (lane 4, 20μg), using PAKγ Antibody (YA7384) . Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST at 25°C for 1hour.The primary antibody (1:2000 dilution) and the loading control antibody (GAPDH, HY-P83944, 1:3000 dilution) were incubated in 5% non-fat milk in TBST for overnight. AF800-conjugated Goat Anti-Rabbit IgG H&L(HY-P810687, 1:1000 dilution) and AF680-conjugated Goat Anti-Mouse IgG H&L(HY-P89186 , 1:10000 dilution) was then applied for 1 hour at 37°C, protect from light.
  • Experimental Validation Results for PAKγ Antibody (YA7384)
  • PAKγ Antibody (YA7384) Product Image
    MedChemExpress Validation

    Western blot analysis was performed on extracts from Hek293 (lane 1, 20μg), HepG2 (lane 2, 20μg), HCT116 (lane 3, 20μg), A594 (lane 4, 20μg), using PAKγ Antibody (YA7384) . Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST at 25°C for 1hour.The primary antibody (1:2000 dilution) and the loading control antibody (GAPDH, HY-P83944, 1:3000 dilution) were incubated in 5% non-fat milk in TBST for overnight. AF800-conjugated Goat Anti-Rabbit IgG H&L(HY-P810687, 1:1000 dilution) and AF680-conjugated Goat Anti-Mouse IgG H&L(HY-P89186 , 1:10000 dilution) was then applied for 1 hour at 37°C, protect from light.

  • PAKγ Antibody (YA7384) Product Image WB