LIMA1 Antibody (YA8156)(PBS only)
(Synonyms: EPLIN; SREBP3)LIMA1 Antibody (YA8156) is a Mouse-derived and non-conjugated IgG1 monoclonal antibody, targeting to LIMA1.
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Host:
Mouse
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Isotype:
IgG1
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Application:
WB, IHC-P
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Reactivity :
Human
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Formulation:
Supplied in PBS, pH 7.4.
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Conjugation:
Non-conjugated
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
Applications
| Application |
WB
WB: Western Blot
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IHC-P
IHC-P: Immunohistochemistry-Paraffin
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|---|---|---|
| Dilution Ratio | 1:1000-2000 | 1:150-500 |
Product Details
LIMA1 Antibody (YA8156) is a Mouse-derived and non-conjugated IgG1 monoclonal antibody, targeting to LIMA1.
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Host Mouse
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Species ReactivityHuman
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Calculated Molecular Weight Predicted band size: 85 kDa
Human recombinant protein fragment corresponding to amino acids 1-275 of human LIMA1 produced in E.coli.
Affinity purified
Non-conjugated
IgG1
Product Properties
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Appearance
Solution
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Formulation
Supplied in PBS, pH 7.4.
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Shipping
Shipping with blue ice.
Background
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Function
LIMA1 is an Actin-binding protein involved in actin cytoskeleton regulation and dynamics. Increases the number and size of actin stress fibers and inhibits membrane ruffling. Inhibits actin filament depolymerization. Bundles actin filaments, delays filament nucleation and reduces formation of branched filaments. Acts as a negative regulator of primary cilium formation. Plays a role in cholesterol homeostasis. Influences plasma cholesterol levels through regulation of intestinal cholesterol absorption. May act as a scaffold protein by regulating NPC1L1 transportation, an essential protein for cholesterol absorption, to the plasma membrane by recruiting MYO5B to NPC1L1, and thus facilitates cholesterol uptake[1][2][3].
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Subcellular Localization
Cytoplasm,Cell junction, focal adhesion,Cytoplasm, cytoskeleton,Cytoplasm, cytoskeleton, stress fiber,Cell membrane,Cell projection, ruffle,Cell projection, lamellipodium
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Expression
Tissue_Specificity: Highly expressed in placenta, kidney, pancreas, prostate, ovary, spleen and heart. Also detected in lung, liver, brain, skeletal muscle, thymus, testis and intestine. Not detected in leukocytes. Isoform Beta expressed generally at very low levels. Isoform Alpha abundant in epithelial cells from mammary gland, prostate and in normal oral keratinocytes. Low levels in aortic endothelial cells and dermal fibroblasts. Not detectable in myocardium
Induction: Down-regulated in some cancer cell lines. Isoform Alpha is induced by serum. Isoform Beta is constitutively expressed -
Isoforms & Post-Translational Modification
Q9UHB6 has 5 isomers: Q9UHB6-1: 85226 Da (predicted); Q9UHB6-2: 67119 Da (predicted); Q9UHB6-3: 51219 Da (predicted); Q9UHB6-4: 85224 Da (predicted); Q9UHB6-5: 67117 Da (predicted).
Ubiquitinated by the ECS(RAB40B) complex leading to its degradation丨Phosphorylation of the C-terminal region by MAPK1/MAPK3 reduces its association with F-actin and contributes to actin filament reorganization and enhances cell motility -
Subunit
Interacts with NPC1L1; bridges NPC1L1 with MYO5B (PubMed:29880681)
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SwissProt ID
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Synonyms
EPLIN; SREBP3
Documentation
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