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  5. Pyruvate Dehydrogenase E1 beta subunit Antibody (YA114)

Pyruvate Dehydrogenase E1 beta subunit Antibody (YA114)

Cat. No.: HY-P80291
COA
SDS
User Guide for Antibodies Technical Support

Pyruvate Dehydrogenase E1 beta subunit Antibody (YA114) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to Pyruvate Dehydrogenase E1 beta subunit.

For research use only. We do not sell to patients.

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Top Publications Citing Use of Products
  • WB: Western Blot;
  • IHC-P: Immunohistochemistry-Paraffin;
  • IHC-F: Immunohistochemistry-Frozen;
  • ICC/IF: Immunocytochemistry/Immunofluorescence;
  • IF-Tissue: Immunofluorescence-Tissue;
  • mIHC: Multiplex Immunohistochemical;
  • IP: Immunoprecipitation;
  • ChIP: Chromatin Immunoprecipitation;
  • FC: Flow Cytometry;
  • ELISA: Enzyme Linked Immunosorbent Assay

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Description

Pyruvate Dehydrogenase E1 beta subunit Antibody (YA114) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to Pyruvate Dehydrogenase E1 beta subunit.
Host

Rabbit
Clonality

Recombinant, Monoclonal
Molecular Weight
Predicted band size: 39 kDa;
Observed band size: 34 kDa
Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.
Species Reactivity
Human, Mouse, Rat
SwissProt ID
Gene ID
Immunogen

Synthetic peptide corresponding to Human Pyruvate Dehydrogenase E1 beta subunit.AA range:310-359.
Application &
Dilution Ratio
Application Dilution Ratio
WB
WB: Western Blot
1:500-1:2000
IHC-P
IHC-P: Immunohistochemistry-Paraffin
1:50-1:200
FC
FC: Flow Cytometry
1:50-1:100
IP
IP: Immunoprecipitation
Use at an assay dependent concentration.
Sensitivity Endogenous Purity Protein A affinity purified.
Conjugation Non-conjugated Modification Unmodified
Isotype IgG  
Appearance

Liquid
Formulation

Supplied in 1*TBS (pH7.4), 0.05% BSA and 40% Glycerol. Preservative: 0.05% Sodium Azide.
Storage & Stability

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.
Shipping

Shipping with blue ice.
Verification Image
ALL WB IHC-P

  • Western blot analysis of extracts from HepG2 (lane 2(20μg) ,Hela (lane 3(20μg)，C2C12 (lane 4(20μg) and HEK293(lane 5(20μg) using Pyruvate Dehydrogenase E1 beta subunit(HY-P80291) Rabbit mAb. Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P80438, 1/10000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Mouse IgG-HRP Secondary Antibody (1/10000) was used for 1 hour at room temperature.

  • Immunohistochemical analysis of paraffin-embedded Mouse brain tissue using Pyruvate Dehydrogenase E1 beta subunit Antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 8 minutes. The tissues were blocked in QuickBlock for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HY-P80291, 1/500) in 4 ℃ overnight. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • Immunohistochemical analysis of paraffin-embedded Mouse brain tissue using Pyruvate Dehydrogenase E1 beta subunit Antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 8 minutes. The tissues were blocked in QuickBlock for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HY-P80291, 1/500) in 4℃ overnight. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Background
Function：The pyruvate dehydrogenase complex catalyzes the overall conversion of pyruvate to acetyl-CoA and CO(2), and thereby links the glycolytic pathway to the tricarboxylic cycle
Subcellular Localization：Mitochondrion matrix
Subunit：Heterotetramer of two PDHA1 and two PDHB subunits (PubMed:12651851, PubMed:17474719, PubMed:19081061, PubMed:29970614). The heterotetramer interacts with DLAT, and is part of the multimeric pyruvate dehydrogenase complex that contains multiple copies of pyruvate dehydrogenase (E1), dihydrolipoamide acetyltransferase (DLAT, E2) and lipoamide dehydrogenase (DLD, E3) (PubMed:14638692). These subunits are bound to an inner core composed of about 48 DLAT and 12 PDHX molecules (PubMed:14638692). Interacts with DLAT (PubMed:20160912)
RRID
Database

Entrez Gene: 5162 Human ; 68263 Mouse ; 289950 Rat

SwissProt: P11177 Human ; Q9D051 Mouse ; P49432 Rat

OMIM: 614111 Human

Research Field

Signal Transduction
Documentation
SDS (251 KB)

COA (206 KB)

User Guide for Antibodies (1077 KB)

Pyruvate Dehydrogenase E1 beta subunit Antibody (YA114) Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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