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Pyruvate Dehydrogenase E1 beta subunit Antibody (YA114)

(Synonyms: PHE1B, PDHB, PDHE1-B)
Customer Review

Based on 1 Customer Validation

Pyruvate Dehydrogenase E1 beta subunit Antibody (YA114) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to Pyruvate Dehydrogenase E1 beta subunit.

For research use only. We do not sell to patients.
  • Host:

    Rabbit

  • Isotype:

    IgG

  • Application:

    WB, IHC-P, FC, IP

  • Reactivity :

    Human, Mouse, Rat

  • Formulation:

    Supplied in 1*TBS (pH7.4), 0.05% BSA and 40% Glycerol. Preservative: 0.05% Sodium Azide.

  • Conjugation:
    Non-conjugated
ALL WB IHC-P FC
  • Experimental Validation Results for Pyruvate Dehydrogenase E1 beta subunit Antibody (YA114)
  • Experimental Validation Results for Pyruvate Dehydrogenase E1 beta subunit Antibody (YA114)
  • Experimental Validation Results for Pyruvate Dehydrogenase E1 beta subunit Antibody (YA114)
  • Experimental Validation Results for Pyruvate Dehydrogenase E1 beta subunit Antibody (YA114)
  • Pyruvate Dehydrogenase E1 beta subunit Antibody (YA114) Product Image
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To place orders, for customer services and technical support, please contact: MedChemExpress USA

Tel: 609-228-6898 E-mail: [email protected] [email protected]

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Use of MCE Products

Applications

Application
WB Info
WB: Western Blot
IHC-P Info
IHC-P: Immunohistochemistry-Paraffin
FC Info
FC: Flow Cytometry
IP Info
IP: Immunoprecipitation
Dilution Ratio 1:500-1:2000 1:50-1:200 1:50-1:100 Use at an assay dependent concentration.

Product Details

Description

Pyruvate Dehydrogenase E1 beta subunit Antibody (YA114) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to Pyruvate Dehydrogenase E1 beta subunit.

  • Host Rabbit
  • Clonality Recombinant,Monoclonal
  • Species Reactivity
    Human, Mouse, Rat
  • Observed Molecular Weight
    Observed band size: 34 kDa Info
    Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.
  • Calculated Molecular Weight Predicted band size: 39 kDa
Species Reactivity Database
Immunogen

Synthetic peptide corresponding to Human Pyruvate Dehydrogenase E1 beta subunit.AA range:310-359.

Sensitivity

Endogenous

Purification

Protein A affinity purified.

Conjugation

Non-conjugated

Modification

Unmodified

Isotype

IgG

RRID

AB_3102878

Product Properties

  • Appearance

    Solution

  • Formulation

    Supplied in 1*TBS (pH7.4), 0.05% BSA and 40% Glycerol. Preservative: 0.05% Sodium Azide.

  • Concentration

    Batch-dependent, Please check the COA for the concentration of each lot. Check Lot Concentration

  • Storage & Stability

    Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

  • Shipping

    Shipping with blue ice.

Verification Images

  • Experimental Validation Results for Pyruvate Dehydrogenase E1 beta subunit Antibody (YA114)
    Western blot analysis of extracts from HepG2 (lane 2(20μg) ,Hela (lane 3(20μg)，C2C12 (lane 4(20μg) and HEK293(lane 5(20μg) using Pyruvate Dehydrogenase E1 beta subunit(HY-P80291) Rabbit mAb. Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P80438, 1/10000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Mouse IgG-HRP Secondary Antibody (1/10000) was used for 1 hour at room temperature.
  • Experimental Validation Results for Pyruvate Dehydrogenase E1 beta subunit Antibody (YA114)
    Immunohistochemical analysis of paraffin-embedded Mouse brain tissue using Pyruvate Dehydrogenase E1 beta subunit Antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 8 minutes. The tissues were blocked in QuickBlock for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HY-P80291, 1/500) in 4 ℃ overnight. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
  • Experimental Validation Results for Pyruvate Dehydrogenase E1 beta subunit Antibody (YA114)
    Immunohistochemical analysis of paraffin-embedded Mouse brain tissue using Pyruvate Dehydrogenase E1 beta subunit Antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 8 minutes. The tissues were blocked in QuickBlock for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HY-P80291, 1/500) in 4℃ overnight. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
  • Experimental Validation Results for Pyruvate Dehydrogenase E1 beta subunit Antibody (YA114)
    Flow cytometric analysis of 1X106 HeLa cells labeling Pyruvate Dehydrogenase E1 beta subunit Antibody(HY-P80291, red). Cells were fixed with 4% paraformaldehyde and permeabilised with 90% methanol. Then stained with the primary antibody at 1/100 dilution for an hour at 4℃. AF488-conjugated Goat Anti-Rabbit IgG H&L (HY-P8002) was used as the secondary antibody at 1/1,000 dilution for 30 minutes at 4℃. Rabbit IgG Isotype Control (HY-P80879, blue) was used as the isotype control, cells without incubation with primary antibody were used as the unlabeled control (black).

Background

  • Function

    Pyruvate Dehydrogenase E1 beta subunit is a The pyruvate dehydrogenase complex catalyzes the overall conversion of pyruvate to acetyl-CoA and CO(2), and thereby links the glycolytic pathway to the tricarboxylic cycle

  • Subcellular Localization

    Mitochondrion matrix

  • Subunit

    Heterotetramer of two PDHA1 and two PDHB subunits (PubMed:12651851, PubMed:17474719, PubMed:19081061, PubMed:29970614). The heterotetramer interacts with DLAT, and is part of the multimeric pyruvate dehydrogenase complex that contains multiple copies of pyruvate dehydrogenase (E1), dihydrolipoamide acetyltransferase (DLAT, E2) and lipoamide dehydrogenase (DLD, E3) (PubMed:14638692). These subunits are bound to an inner core composed of about 48 DLAT and 12 PDHX molecules (PubMed:14638692). Interacts with DLAT (PubMed:20160912)

  • SwissProt ID

    P11177

  • Gene ID
    5162 [NCBI]
  • Synonyms

    PHE1B, PDHB, PDHE1-B

  • Research Field

    Signal Transduction

Pyruvate Dehydrogenase E1 beta subunit Antibody (YA114) Related Classifications

MOQ
Minimum order quantity
100 mg

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Measured by its ability to inhibit anti-CD3-induced proliferation of stimulated CTLL-2 mouse cytotoxic T cells. The ED50 this effect is 0.8023 μg/ml, corresponding to a specific activity is 1246.42 units/mg.

Assay Types
  • 1/16

    MedchemExpress Validation
     Western blot analysis of extracts from HepG2 (lane 2(20μg) ,Hela (lane 3(20μg)，C2C12 (lane 4(20μg) and HEK293(lane 5(20μg) using Pyruvate Dehydrogenase E1 beta subunit(HY-P80291) Rabbit mAb. Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P80438, 1/10000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Mouse IgG-HRP Secondary Antibody (1/10000) was used for 1 hour at room temperature.

  • 1/16

    MedchemExpress Validation
     Immunohistochemical analysis of paraffin-embedded Mouse brain tissue using Pyruvate Dehydrogenase E1 beta subunit Antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 8 minutes. The tissues were blocked in QuickBlock for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HY-P80291, 1/500) in 4 ℃ overnight. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • 1/16

    MedchemExpress Validation
     Immunohistochemical analysis of paraffin-embedded Mouse brain tissue using Pyruvate Dehydrogenase E1 beta subunit Antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 8 minutes. The tissues were blocked in QuickBlock for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HY-P80291, 1/500) in 4℃ overnight. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • 1/16

    MedChemExpress Validation
     Flow cytometric analysis of 1X106 HeLa cells labeling Pyruvate Dehydrogenase E1 beta subunit Antibody(HY-P80291, red). Cells were fixed with 4% paraformaldehyde and permeabilised with 90% methanol. Then stained with the primary antibody at 1/100 dilution for an hour at 4℃. AF488-conjugated Goat Anti-Rabbit IgG H&L (HY-P8002) was used as the secondary antibody at 1/1,000 dilution for 30 minutes at 4℃. Rabbit IgG Isotype Control (HY-P80879, blue) was used as the isotype control, cells without incubation with primary antibody were used as the unlabeled control (black).

  • Pyruvate Dehydrogenase E1 beta subunit Antibody (YA114) Product Image
    1/16
  • Experimental Validation Results for Pyruvate Dehydrogenase E1 beta subunit Antibody (YA114)
  • Experimental Validation Results for Pyruvate Dehydrogenase E1 beta subunit Antibody (YA114)
  • Experimental Validation Results for Pyruvate Dehydrogenase E1 beta subunit Antibody (YA114)
  • Experimental Validation Results for Pyruvate Dehydrogenase E1 beta subunit Antibody (YA114)
  • Pyruvate Dehydrogenase E1 beta subunit Antibody (YA114) Product Image
  • Experimental Validation Results for Pyruvate Dehydrogenase E1 beta subunit Antibody (YA114)
    1/1
    MedchemExpress Validation
     Western blot analysis of extracts from HepG2 (lane 2(20μg) ,Hela (lane 3(20μg)，C2C12 (lane 4(20μg) and HEK293(lane 5(20μg) using Pyruvate Dehydrogenase E1 beta subunit(HY-P80291) Rabbit mAb. Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P80438, 1/10000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Mouse IgG-HRP Secondary Antibody (1/10000) was used for 1 hour at room temperature.
  • Experimental Validation Results for Pyruvate Dehydrogenase E1 beta subunit Antibody (YA114)
    1/1
    MedchemExpress Validation
     Immunohistochemical analysis of paraffin-embedded Mouse brain tissue using Pyruvate Dehydrogenase E1 beta subunit Antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 8 minutes. The tissues were blocked in QuickBlock for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HY-P80291, 1/500) in 4 ℃ overnight. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
  • Experimental Validation Results for Pyruvate Dehydrogenase E1 beta subunit Antibody (YA114)
    1/1
    MedchemExpress Validation
     Immunohistochemical analysis of paraffin-embedded Mouse brain tissue using Pyruvate Dehydrogenase E1 beta subunit Antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 8 minutes. The tissues were blocked in QuickBlock for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HY-P80291, 1/500) in 4℃ overnight. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
  • Experimental Validation Results for Pyruvate Dehydrogenase E1 beta subunit Antibody (YA114)
    1/1
    MedChemExpress Validation
     Flow cytometric analysis of 1X106 HeLa cells labeling Pyruvate Dehydrogenase E1 beta subunit Antibody(HY-P80291, red). Cells were fixed with 4% paraformaldehyde and permeabilised with 90% methanol. Then stained with the primary antibody at 1/100 dilution for an hour at 4℃. AF488-conjugated Goat Anti-Rabbit IgG H&L (HY-P8002) was used as the secondary antibody at 1/1,000 dilution for 30 minutes at 4℃. Rabbit IgG Isotype Control (HY-P80879, blue) was used as the isotype control, cells without incubation with primary antibody were used as the unlabeled control (black).
  • Experimental Validation Results for Pyruvate Dehydrogenase E1 beta subunit Antibody (YA114)
  • Experimental Validation Results for Pyruvate Dehydrogenase E1 beta subunit Antibody (YA114)
  • Experimental Validation Results for Pyruvate Dehydrogenase E1 beta subunit Antibody (YA114)
  • Experimental Validation Results for Pyruvate Dehydrogenase E1 beta subunit Antibody (YA114)
  • Pyruvate Dehydrogenase E1 beta subunit Antibody (YA114) Product Image
    MedchemExpress Validation

    Western blot analysis of extracts from HepG2 (lane 2(20μg) ,Hela (lane 3(20μg)，C2C12 (lane 4(20μg) and HEK293(lane 5(20μg) using Pyruvate Dehydrogenase E1 beta subunit(HY-P80291) Rabbit mAb. Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P80438, 1/10000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Mouse IgG-HRP Secondary Antibody (1/10000) was used for 1 hour at room temperature.

  • Pyruvate Dehydrogenase E1 beta subunit Antibody (YA114) Product Image
    MedchemExpress Validation

    Immunohistochemical analysis of paraffin-embedded Mouse brain tissue using Pyruvate Dehydrogenase E1 beta subunit Antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 8 minutes. The tissues were blocked in QuickBlock for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HY-P80291, 1/500) in 4 ℃ overnight. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • Pyruvate Dehydrogenase E1 beta subunit Antibody (YA114) Product Image
    MedchemExpress Validation

    Immunohistochemical analysis of paraffin-embedded Mouse brain tissue using Pyruvate Dehydrogenase E1 beta subunit Antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 8 minutes. The tissues were blocked in QuickBlock for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HY-P80291, 1/500) in 4℃ overnight. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • Pyruvate Dehydrogenase E1 beta subunit Antibody (YA114) Product Image
    MedChemExpress Validation

    Flow cytometric analysis of 1X106 HeLa cells labeling Pyruvate Dehydrogenase E1 beta subunit Antibody(HY-P80291, red). Cells were fixed with 4% paraformaldehyde and permeabilised with 90% methanol. Then stained with the primary antibody at 1/100 dilution for an hour at 4℃. AF488-conjugated Goat Anti-Rabbit IgG H&L (HY-P8002) was used as the secondary antibody at 1/1,000 dilution for 30 minutes at 4℃. Rabbit IgG Isotype Control (HY-P80879, blue) was used as the isotype control, cells without incubation with primary antibody were used as the unlabeled control (black).

  • Pyruvate Dehydrogenase E1 beta subunit Antibody (YA114) Product Image WB
  • Pyruvate Dehydrogenase E1 beta subunit Antibody (YA114) Product Image IHC-P
  • Pyruvate Dehydrogenase E1 beta subunit Antibody (YA114) Product Image IHC-P
  • Pyruvate Dehydrogenase E1 beta subunit Antibody (YA114) Product Image FC