Estrogen Receptor beta Antibody (YA5491)
(Synonyms: Estrogen receptor beta; ER-beta; Nuclear receptor subfamily 3 group A member 2; )Estrogen Receptor beta Antibody (YA5491) is a Mouse-derived and non-conjugated IgG2b monoclonal antibody, targeting to Estrogen Receptor beta.
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Host:
Mouse
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Isotype:
IgG2b
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Application:
WB, ICC/IF, ELISA
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Reactivity :
Human
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Formulation:
Supplied in PBS, 50% glycerol, 0.05% Proclin 300, 0.05%BSA
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Conjugation:
Non-conjugated
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Applications
| Application |
WB
WB: Western Blot
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ICC/IF
ICC/IF: Immunocytochemistry/
Immunofluorescence |
ELISA
ELISA: Enzyme Linked Immunosorbent Assay
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|---|---|---|---|
| Dilution Ratio | 1:500-2000 | 1:100-500 | 1:1000-5000 |
Product Details
Estrogen Receptor beta Antibody (YA5491) is a Mouse-derived and non-conjugated IgG2b monoclonal antibody, targeting to Estrogen Receptor beta.
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Host Mouse
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Clonality Monoclonal
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Species ReactivityHuman
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Observed Molecular WeightObserved band size: 59 kDa
Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.
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Calculated Molecular Weight Predicted band size: 59 kDa
Synthesized peptide derived from human Estrogen Receptor β AA range: 400-530
Protein G
Non-conjugated
Unmodified
IgG2b
Product Properties
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Appearance
Liquid
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Formulation
Supplied in PBS, 50% glycerol, 0.05% Proclin 300, 0.05%BSA
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Storage & Stability
Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.
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Shipping
Shipping with blue ice.
Background
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Function
Estrogen Receptor beta is a Nuclear hormone receptor. Binds estrogens with an affinity similar to that of ESR1/ER-alpha, and activates expression of reporter genes containing estrogen response elements (ERE) in an estrogen-dependent manner; Lacks ligand binding ability and has no or only very low ERE binding activity resulting in the loss of ligand-dependent transactivation ability[1].
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Subcellular Localization
Nucleus
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Expression
Tissue_specificity:It is expressed in the testes and ovaries, with lower expression levels in the heart, brain, placenta, liver, skeletal muscle, spleen, thymus, prostate, colon, bone marrow, breast, and uterus. It is also present in uterine bone, breast, and ovarian tumor cell lines, but not in colon and liver tumors; it is expressed in the spleen, thymus, testes, and ovaries, with lower expression levels in skeletal muscle, prostate, colon, small intestine, leukocytes, bone marrow, breast, and uterus; it is expressed in the testes; it is expressed in the testes, with lower expression levels in the spleen, thymus, ovaries, breast, and uterus; it is expressed in the testes, placenta, skeletal muscle, spleen, and leukocytes, with lower expression levels in the heart, lung, liver, kidney, pancreas, thymus, prostate, colon, small intestine, bone marrow, breast, and uterus. It is not expressed in brain tissue. -
Isoforms & Post-Translational Modification
Q92731 has 9 isomers: Q92731-1: 59216 Da (predicted); Q92731-2: 55483 Da (predicted); Q92731-3: 35944 Da (predicted); Q92731-4: 57518 Da (predicted); Q92731-5: 54096 Da (predicted); Q92731-6: 52959 Da (predicted); Q92731-7: 48614 Da (predicted); Q92731-8: 41948 Da (predicted); Q92731-9: 53209 Da (predicted).
Phosphorylation at Ser-87 and Ser-105 recruits NCOA1 -
Subunit
Binds DNA as a homodimer. Can form a heterodimer with ESR1. Interacts with NCOA1, NCOA3, NCOA5 and NCOA6 coactivators, leading to a strong increase of transcription of target genes. Interacts with UBE1C. Interacts with AKAP13. Interacts with DNTTIP2. Interacts with isoform 4 of TXNRD1. Interacts with CCDC62 in the presence of estradiol/E2; this interaction seems to enhance the transcription of target genes, including cyclin-D1/CCND1 AP-1 promoter. Interacts with DNAAF4 (By similarity). Interacts with PRMT2. Interacts with CCAR2 (via N-terminus) in a ligand-independent manner. Interacts with RBM39, in the presence of estradiol (E2) (By similarity). Interacts with STUB1/CHIP (By similarity)
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SwissProt ID
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Synonyms
Estrogen receptor beta; ER-beta; Nuclear receptor subfamily 3 group A member 2;
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