Estrogen Receptor beta Antibody (YA5491)

Cat. No.: HY-P85799: Data Sheet User Guide for Antibodies
FAQs
Technical Support

Estrogen Receptor beta Antibody (YA5491) is a Mouse-derived and non-conjugated IgG2b monoclonal antibody, targeting to Estrogen Receptor beta.

For research use only. We do not sell to patients.

Size		Stock
50 μL		Get quote
100 μL		Get quote

* Please select Quantity before adding items.

WB: Western Blot;
IHC-P: Immunohistochemistry-Paraffin;
IHC-F: Immunohistochemistry-Frozen;
ICC/IF: Immunocytochemistry/Immunofluorescence;
IF-Tissue: Immunofluorescence-Tissue;
mIHC: Multiplex Immunohistochemical;
IP: Immunoprecipitation;
ChIP: Chromatin Immunoprecipitation;
FC: Flow Cytometry;
ELISA: Enzyme Linked Immunosorbent Assay

Product Detail
Background
Documentation
References

Description

Estrogen Receptor beta Antibody (YA5491) is a Mouse-derived and non-conjugated IgG2b monoclonal antibody, targeting to Estrogen Receptor beta.

Host

Mouse

Clonality

Monoclonal

Molecular Weight

Predicted band size: 59 kDa;

Observed band size: 59 kDa

Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.

Species Reactivity

Human

SwissProt ID

Q92731

Gene ID

2100 [NCBI]

Immunogen

Synthesized peptide derived from human Estrogen Receptor β AA range: 400-530

Application &
Dilution Ratio

Application	Dilution Ratio
WB WB: Western Blot	1:500-2000
ICC/IF ICC/IF: Immunocytochemistry/Immunofluorescence	1:100-500
ELISA ELISA: Enzyme Linked Immunosorbent Assay	1:1000-5000

Purity	Protein G	Conjugation	Non-conjugated
Modification	Unmodified	Isotype	IgG2b

Appearance

Liquid

Formulation

Supplied in PBS, 50% glycerol, 0.05% Proclin 300, 0.05%BSA

Storage & Stability

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

Shipping

Shipping with blue ice.

Background

Function：Nuclear hormone receptor. Binds estrogens with an affinity similar to that of ESR1/ER-alpha, and activates expression of reporter genes containing estrogen response elements (ERE) in an estrogen-dependent manner (PubMed:20074560); Lacks ligand binding ability and has no or only very low ERE binding activity resulting in the loss of ligand-dependent transactivation ability

Subcellular Localization：Nucleus

Expression：
Tissue_specificity:It is expressed in the testes and ovaries, with lower expression levels in the heart, brain, placenta, liver, skeletal muscle, spleen, thymus, prostate, colon, bone marrow, breast, and uterus. It is also present in uterine bone, breast, and ovarian tumor cell lines, but not in colon and liver tumors; it is expressed in the spleen, thymus, testes, and ovaries, with lower expression levels in skeletal muscle, prostate, colon, small intestine, leukocytes, bone marrow, breast, and uterus; it is expressed in the testes; it is expressed in the testes, with lower expression levels in the spleen, thymus, ovaries, breast, and uterus; it is expressed in the testes, placenta, skeletal muscle, spleen, and leukocytes, with lower expression levels in the heart, lung, liver, kidney, pancreas, thymus, prostate, colon, small intestine, bone marrow, breast, and uterus. It is not expressed in brain tissue.

Isoforms & Post-Translational Modification：Q92731 has 9 isomers: Q92731-1: 59216 Da (predicted); Q92731-2: 55483 Da (predicted); Q92731-3: 35944 Da (predicted); Q92731-4: 57518 Da (predicted); Q92731-5: 54096 Da (predicted); Q92731-6: 52959 Da (predicted); Q92731-7: 48614 Da (predicted); Q92731-8: 41948 Da (predicted); Q92731-9: 53209 Da (predicted).
Phosphorylation at Ser-87 and Ser-105 recruits NCOA1

Subunit：Binds DNA as a homodimer. Can form a heterodimer with ESR1. Interacts with NCOA1, NCOA3, NCOA5 and NCOA6 coactivators, leading to a strong increase of transcription of target genes. Interacts with UBE1C. Interacts with AKAP13. Interacts with DNTTIP2. Interacts with isoform 4 of TXNRD1. Interacts with CCDC62 in the presence of estradiol/E2; this interaction seems to enhance the transcription of target genes, including cyclin-D1/CCND1 AP-1 promoter. Interacts with DNAAF4 (By similarity). Interacts with PRMT2. Interacts with CCAR2 (via N-terminus) in a ligand-independent manner. Interacts with RBM39, in the presence of estradiol (E2) (By similarity). Interacts with STUB1/CHIP (By similarity)

Synonyms

Estrogen receptor beta; ER-beta; Nuclear receptor subfamily 3 group A member 2;

Documentation

User Guide for Antibodies (1077 KB)

References

[1]. /biology-dictionary/pc-9-cell.html [Content Brief]