LIN28A Antibody (YA711)

Cat. No.: HY-P80744: Data Sheet COA
SDS
User Guide for Antibodies
FAQs
Technical Support

LIN28A Antibody (YA711) is a Mouse-derived and non-conjugated IgG2a monoclonal antibody, targeting to LIN28A.

For research use only. We do not sell to patients.

Size	Stock	Quantity
20 μL	In-stock	Estimated Time of Arrival: December 31
50 μL	In-stock	Estimated Time of Arrival: December 31
100 μL	In-stock	Estimated Time of Arrival: December 31
250 μL	Get quote

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LIN28A Antibody (YA711) Featured Recommendations:

WB: Western Blot;
IHC-P: Immunohistochemistry-Paraffin;
IHC-F: Immunohistochemistry-Frozen;
ICC/IF: Immunocytochemistry/Immunofluorescence;
IF-Tissue: Immunofluorescence-Tissue;
mIHC: Multiplex Immunohistochemical;
IP: Immunoprecipitation;
ChIP: Chromatin Immunoprecipitation;
FC: Flow Cytometry;
ELISA: Enzyme Linked Immunosorbent Assay

Product Detail
Background
Documentation

Description

LIN28A Antibody (YA711) is a Mouse-derived and non-conjugated IgG2a monoclonal antibody, targeting to LIN28A.

Host

Mouse

Clonality

Monoclonal

Molecular Weight

Predicted band size: 23 kDa;

Observed band size: 26 kDa

Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.

Species Reactivity

Human, Mouse

SwissProt ID

Q9H9Z2

Gene ID

79727 [NCBI]

Immunogen

Synthetic peptide corresponding to Human LIN28A.The exact sequence is proprietary to MCE.

Application &
Dilution Ratio

Application	Dilution Ratio
WB WB: Western Blot	1:500-1:1000

Sensitivity	Endogenous	Purity	affinity purified
Conjugation	Non-conjugated	Modification	Unmodified
Isotype	IgG2a

Appearance

Liquid

Formulation

Supplied in 1*PBS (pH 7.3), 50% glycerol and 0.5% BSA. Preservative: 0.02% sodium azide.

Storage & Stability

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

Shipping

Shipping with blue ice.

Background

Function：RNA-binding protein that inhibits processing of pre-let-7 miRNAs and regulates translation of mRNAs that control developmental timing, pluripotency and metabolism (PubMed:21247876). Seems to recognize a common structural G-quartet (G4) feature in its miRNA and mRNA targets (Probable). 'Translational enhancer' that drives specific mRNAs to polysomes and increases the efficiency of protein synthesis. Its association with the translational machinery and target mRNAs results in an increased number of initiation events per molecule of mRNA and, indirectly, in mRNA stabilization. Binds IGF2 mRNA, MYOD1 mRNA, ARBP/36B4 ribosomal protein mRNA and its own mRNA. Essential for skeletal muscle differentiation program through the translational up-regulation of IGF2 expression. Suppressor of microRNA (miRNA) biogenesis, including that of let-7, miR107, miR-143 and miR-200c. Specifically binds the miRNA precursors (pre-miRNAs), recognizing an 5'-GGAG-3' motif found in pre-miRNA terminal loop, and recruits TUT4 and TUT7 uridylyltransferases (PubMed:18951094, PubMed:19703396, PubMed:22118463, PubMed:22898984). This results in the terminal uridylation of target pre-miRNAs (PubMed:18951094, PubMed:19703396, PubMed:22118463, PubMed:22898984). Uridylated pre-miRNAs fail to be processed by Dicer and undergo degradation. The repression of let-7 expression is required for normal development and contributes to maintain the pluripotent state by preventing let-7-mediated differentiation of embryonic stem cells (PubMed:18951094, PubMed:19703396, PubMed:22118463, PubMed:22898984). Localized to the periendoplasmic reticulum area, binds to a large number of spliced mRNAs and inhibits the translation of mRNAs destined for the ER, reducing the synthesis of transmembrane proteins, ER or Golgi lumen proteins, and secretory proteins. Binds to and enhances the translation of mRNAs for several metabolic enzymes, such as PFKP, PDHA1 or SDHA, increasing glycolysis and oxidative phosphorylation. Which, with the let-7 repression may enhance tissue repair in adult tissue (By similarity)

Subcellular Localization：Cytoplasm; Rough endoplasmic reticulum; Cytoplasm, P-body; Cytoplasm, Stress granule; Nucleus, nucleolus

Expression：
Tissue_specificity:It is expressed in embryonic stem cells, placenta, and testes. It is often upregulated in breast tumors that overexpress HER2.

Induction:Can be negatively regulated by the interaction of microRNAs miR-125a and miR-125b with at least two miRNA responsive elements (miREs) in the 3'-UTR of this gene. These interactions may reduce both translation efficiency and mRNA abundance. Negatively regulated by retinoic acid

Subunit：Monomer (By similarity). During skeletal muscle differentiation, associated with translation initiation complexes in the polysomal compartment (PubMed:21247876). Directly interacts with EIF3S2 (By similarity). Interacts with NCL in an RNA-dependent manner (By similarity). Interacts (via C-terminus) with DHX9 (via N- and C-terminus); this interaction occurs in a RNA-independent manner (PubMed:21247876). Interacts with TUT4 in the presence of pre-let-7 RNA (PubMed:19703396, PubMed:22118463)

Database

Entrez Gene: 79727 Human ; 83557 Mouse ;

SwissProt: Q9H9Z2 Human ; Q8K3Y3 Mouse ;

OMIM: 611043 Human

Research Field

Epigenetics and Nuclear Signaling

Synonyms

CSDD1; FLJ12457; LIN 28; Lin-28A; LIN28; LIN28A; LN28A_HUMAN; Protein lin-28 homolog A; ZCCHC1; Zinc finger CCHC domain-containing protein 1.

Documentation

Select Batch:

Data Sheet (233 KB) SDS (252 KB)

COA (205 KB)

User Guide for Antibodies (1077 KB)

LIN28A Antibody (YA711) Related Classifications

Help & FAQs

Do most proteins show cross-species activity?
Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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LIN28A Antibody (YA711)

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Background

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LIN28A Antibody (YA711) Related Classifications

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