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NGF Antibody (YA3533)

(Synonyms: Beta nerve growth factor antibody; Beta NGF antibody; Beta-nerve growth factor antibody; Beta-NGF antibody; HSAN5 antibody; MGC161426 antibody; MGC161428 antibody; Nerve growth factor (beta polypeptide) antibody; Nerve growth factor antibody; Nerve growth factor beta antibody; Nerve growth factor beta polypeptide antibody; Nerve growth factor beta subunit antibody; NGF antibody; NGF_HUMAN antibody; NGFB antibody; NID67 antibody; )
Revisión del cliente

Based on 1 Customer Validation

NGF Antibody (YA3533) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to NGF.

For research use only. We do not sell to patients.
  • Host:

    Rabbit

  • Isotype:

    IgG

  • Application:

    WB, IHC-P, IHC-F, ICC/IF, IF-Tissue

  • Reactivity :

    Human, Mouse, Rat, Zebrafish

  • Formulation:

    Supplied in 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

  • Conjugation:
    Non-conjugated
ALL WB IHC-P ICC
  • Experimental Validation Results for NGF Antibody (YA3533)
  • Experimental Validation Results for NGF Antibody (YA3533)
  • Experimental Validation Results for NGF Antibody (YA3533)
  • Experimental Validation Results for NGF Antibody (YA3533)
  • Experimental Validation Results for NGF Antibody (YA3533)
  • NGF Antibody (YA3533) Product Image
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To place orders, for customer services and technical support, please contact: MedChemExpress USA

Tel: 609-228-6898 E-mail: [email protected] [email protected]

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Applications

Application
WB Info
WB: Western Blot
ICC/IF Info
ICC/IF: Immunocytochemistry/
Immunofluorescence
IF-Tissue Info
IF-Tissue: Immunofluorescence-Tissue
IHC-P Info
IHC-P: Immunohistochemistry-Paraffin
IHC-F Info
IHC-F: Immunohistochemistry-Frozen
Dilution Ratio 1:2000 1:50-1:200 1:50-1:200 1:50-1:100 1:100

Product Details

Descripciòn

NGF Antibody (YA3533) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to NGF.

  • Host Rabbit
  • Clonality Monoclonal,Recombinant
  • Species Reactivity
    Human, Mouse, Rat, Zebrafish
  • Observed Molecular Weight
    Observed band size: 35 kDa Info
    Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.
  • Calculated Molecular Weight Predicted band size: 27 kDa
Species Reactivity Database
Immunogen

Synthetic peptide within Human NGF aa 192-241 / 241.

Sensitivity

Endogenous

Purification

affinity purified

Conjugation

Non-conjugated

Modification

Unmodified

Isotype

IgG

RRID

AB_3103222

Product Properties

  • Appearance

    Liquid

  • Formulation

    Supplied in 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

  • Concentration

    Batch-dependent, Please check the COA for the concentration of each lot. Check Lot Concentration

  • Storage & Stability

    Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

  • Envío

    Shipping with blue ice.

Verification Images

  • Experimental Validation Results for NGF Antibody (YA3533)
    Western blot analysis of extracts from HL-60(lane 2, 20μg), HeLa(lane 3, 20μg), MDA-MB-231(lane 4, 20μg) and NIH-3T3(lane 5, 20μg) using NGF Antibody (HY-P81121). Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hours at room temperature. The primary antibody (1/2000) and Loading control antibody (Beta Actin, HY-P80993, 1/10000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Rabbit IgG-HRP Secondary Antibody (HY-P8001, 1/10000) was used for 1 hour at room temperature.
  • Experimental Validation Results for NGF Antibody (YA3533)
    Immunohistochemical analysis of paraffin-embedded Mouse brain tissue using NGF Antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0) for 8 minutes. The tissues were blocked in QuickBlock for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HY-P81121, 1:100) in 4℃ overnight. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
  • Experimental Validation Results for NGF Antibody (YA3533)
    Immunohistochemical analysis of paraffin-embedded Mouse brain tissue using NGF Antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0) for 8 minutes. The tissues were blocked in QuickBlock for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HY-P81121, 1:100) in 4℃ overnight. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
  • Experimental Validation Results for NGF Antibody (YA3533)
    Immunocytochemistry analysis of SH-SY-5Y cells labeling NGF with NGF antibody (HY-P81121) at 1/50 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 for 10 minutes at room temperature, then blocked with QuickBlock™ Blocking Buffer for Immunol Staining for 10 min at room temperature. Cells were then incubated with NGF antibody (HY-P81121) at 1/50 dilution in QuickBlock™ Blocking Buffer for Immunol Staining at 4 ℃. Alexa Fluor® 488-conjugated AffiniPure Goat Anti-Rabbit IgG H&L（HY-P8002, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).
  • Experimental Validation Results for NGF Antibody (YA3533)
    Immunocytochemistry analysis of SH-SY-5Y cells labeling NGF with NGF antibody (HY-P81121) at 1/200 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 for 10 minutes at room temperature, then blocked with QuickBlock™ Blocking Buffer for Immunol Staining for 10 min at room temperature. Cells were then incubated with NGF antibody (HY-P81121) at 1/200 dilution in QuickBlock™ Blocking Buffer for Immunol Staining at 4 ℃. Alexa Fluor® 488-conjugated AffiniPure Goat Anti-Rabbit IgG H&L（HY-P8002, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).

Background

  • Function

    Nerve growth factor is important for the development and maintenance of the sympathetic and sensory nervous systems (PubMed:14976160, PubMed:20978020). Extracellular ligand for the NTRK1 and NGFR receptors, activates cellular signaling cascades to regulate neuronal proliferation, differentiation and survival (Probable) (PubMed:20978020). The immature NGF precursor (proNGF) functions as a ligand for the heterodimeric receptor formed by SORCS2 and NGFR, and activates cellular signaling cascades that lead to inactivation of RAC1 and/or RAC2, reorganization of the actin cytoskeleton and neuronal growth cone collapse. In contrast to mature NGF, the precursor form (proNGF) promotes neuronal apoptosis (in vitro) (By similarity). Inhibits metalloproteinase-dependent proteolysis of platelet glycoprotein VI (PubMed:20164177). Binds lysophosphatidylinositol and lysophosphatidylserine between the two chains of the homodimer. The lipid-bound form promotes histamine relase from mast cells, contrary to the lipid-free form (By similarity)

  • Subcellular Localization

    Secreted; Endosome lumen

  • Subunit

    Homodimer (PubMed:10490030, PubMed:15131306, PubMed:17196528). The homodimer interacts with a single NTRK1 chain (PubMed:10490030, PubMed:17196528). The homodimer interacts with a single NGFR chain (PubMed:15131306). The NGF dimer interacts with a single SORCS2 chain (via extracellular domain) (By similarity). The NGF precursor (proNGF) binds to a receptor complex formed by SORT1 and NGFR, which leads to NGF endocytosis. Both mature NGF and the immature NGF precursor (proNGF) interact with SORCS2 and with the heterodimer formed by SORCS2 and NGFR (via extracellular domains) (By similarity). The NGF precursor (proNGF) has much higher affinity for SORCS2 than mature NGF. The NGF precursor (proNGF) has much higher affinity for SORT1 than mature NGF (By similarity). Interacts with ADAM10 in a divalent cation-dependent manner (PubMed:20164177). Interaction with SORCS3 (PubMed:15710408)

  • SwissProt ID

    P01138

  • Gene ID
    4803 [NCBI]
  • Synonyms

    Beta nerve growth factor antibody; Beta NGF antibody; Beta-nerve growth factor antibody; Beta-NGF antibody; HSAN5 antibody; MGC161426 antibody; MGC161428 antibody; Nerve growth factor (beta polypeptide) antibody; Nerve growth factor antibody; Nerve growth factor beta antibody; Nerve growth factor beta polypeptide antibody; Nerve growth factor beta subunit antibody; NGF antibody; NGF_HUMAN antibody; NGFB antibody; NID67 antibody;

NGF Antibody (YA3533) Related Classifications

MOQ
Minimum order quantity
100 mg

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Measured by its ability to inhibit anti-CD3-induced proliferation of stimulated CTLL-2 mouse cytotoxic T cells. The ED50 this effect is 0.8023 μg/ml, corresponding to a specific activity is 1246.42 units/mg.

Assay Types
  • 1/16

    MedChemExpress Validation
     Western blot analysis of extracts from HL-60(lane 2, 20μg), HeLa(lane 3, 20μg), MDA-MB-231(lane 4, 20μg) and NIH-3T3(lane 5, 20μg) using NGF Antibody (HY-P81121). Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hours at room temperature. The primary antibody (1/2000) and Loading control antibody (Beta Actin, HY-P80993, 1/10000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Rabbit IgG-HRP Secondary Antibody (HY-P8001, 1/10000) was used for 1 hour at room temperature.

  • 1/16

    MedchemExpress Validation
     Immunohistochemical analysis of paraffin-embedded Mouse brain tissue using NGF Antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0) for 8 minutes. The tissues were blocked in QuickBlock for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HY-P81121, 1:100) in 4℃ overnight. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • 1/16

    MedchemExpress Validation
     Immunohistochemical analysis of paraffin-embedded Mouse brain tissue using NGF Antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0) for 8 minutes. The tissues were blocked in QuickBlock for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HY-P81121, 1:100) in 4℃ overnight. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • 1/16

    MedchemExpress Validation
     Immunocytochemistry analysis of SH-SY-5Y cells labeling NGF with NGF antibody (HY-P81121) at 1/50 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 for 10 minutes at room temperature, then blocked with QuickBlock™ Blocking Buffer for Immunol Staining for 10 min at room temperature. Cells were then incubated with NGF antibody (HY-P81121) at 1/50 dilution in QuickBlock™ Blocking Buffer for Immunol Staining at 4 ℃. Alexa Fluor® 488-conjugated AffiniPure Goat Anti-Rabbit IgG H&L（HY-P8002, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).

  • 1/16

    MedchemExpress Validation
     Immunocytochemistry analysis of SH-SY-5Y cells labeling NGF with NGF antibody (HY-P81121) at 1/200 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 for 10 minutes at room temperature, then blocked with QuickBlock™ Blocking Buffer for Immunol Staining for 10 min at room temperature. Cells were then incubated with NGF antibody (HY-P81121) at 1/200 dilution in QuickBlock™ Blocking Buffer for Immunol Staining at 4 ℃. Alexa Fluor® 488-conjugated AffiniPure Goat Anti-Rabbit IgG H&L（HY-P8002, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).

  • NGF Antibody (YA3533) Product Image
    1/16
  • Experimental Validation Results for NGF Antibody (YA3533)
  • Experimental Validation Results for NGF Antibody (YA3533)
  • Experimental Validation Results for NGF Antibody (YA3533)
  • Experimental Validation Results for NGF Antibody (YA3533)
  • Experimental Validation Results for NGF Antibody (YA3533)
  • NGF Antibody (YA3533) Product Image
  • Experimental Validation Results for NGF Antibody (YA3533)
    1/1
    MedChemExpress Validation
     Western blot analysis of extracts from HL-60(lane 2, 20μg), HeLa(lane 3, 20μg), MDA-MB-231(lane 4, 20μg) and NIH-3T3(lane 5, 20μg) using NGF Antibody (HY-P81121). Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hours at room temperature. The primary antibody (1/2000) and Loading control antibody (Beta Actin, HY-P80993, 1/10000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Rabbit IgG-HRP Secondary Antibody (HY-P8001, 1/10000) was used for 1 hour at room temperature.
  • Experimental Validation Results for NGF Antibody (YA3533)
    1/1
    MedchemExpress Validation
     Immunohistochemical analysis of paraffin-embedded Mouse brain tissue using NGF Antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0) for 8 minutes. The tissues were blocked in QuickBlock for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HY-P81121, 1:100) in 4℃ overnight. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
  • Experimental Validation Results for NGF Antibody (YA3533)
    1/1
    MedchemExpress Validation
     Immunohistochemical analysis of paraffin-embedded Mouse brain tissue using NGF Antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0) for 8 minutes. The tissues were blocked in QuickBlock for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HY-P81121, 1:100) in 4℃ overnight. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
  • Experimental Validation Results for NGF Antibody (YA3533)
    1/1
    MedchemExpress Validation
     Immunocytochemistry analysis of SH-SY-5Y cells labeling NGF with NGF antibody (HY-P81121) at 1/50 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 for 10 minutes at room temperature, then blocked with QuickBlock™ Blocking Buffer for Immunol Staining for 10 min at room temperature. Cells were then incubated with NGF antibody (HY-P81121) at 1/50 dilution in QuickBlock™ Blocking Buffer for Immunol Staining at 4 ℃. Alexa Fluor® 488-conjugated AffiniPure Goat Anti-Rabbit IgG H&L（HY-P8002, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).
  • Experimental Validation Results for NGF Antibody (YA3533)
    1/1
    MedchemExpress Validation
     Immunocytochemistry analysis of SH-SY-5Y cells labeling NGF with NGF antibody (HY-P81121) at 1/200 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 for 10 minutes at room temperature, then blocked with QuickBlock™ Blocking Buffer for Immunol Staining for 10 min at room temperature. Cells were then incubated with NGF antibody (HY-P81121) at 1/200 dilution in QuickBlock™ Blocking Buffer for Immunol Staining at 4 ℃. Alexa Fluor® 488-conjugated AffiniPure Goat Anti-Rabbit IgG H&L（HY-P8002, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).
  • Experimental Validation Results for NGF Antibody (YA3533)
  • Experimental Validation Results for NGF Antibody (YA3533)
  • Experimental Validation Results for NGF Antibody (YA3533)
  • Experimental Validation Results for NGF Antibody (YA3533)
  • Experimental Validation Results for NGF Antibody (YA3533)
  • NGF Antibody (YA3533) Product Image
    MedChemExpress Validation

    Western blot analysis of extracts from HL-60(lane 2, 20μg), HeLa(lane 3, 20μg), MDA-MB-231(lane 4, 20μg) and NIH-3T3(lane 5, 20μg) using NGF Antibody (HY-P81121). Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hours at room temperature. The primary antibody (1/2000) and Loading control antibody (Beta Actin, HY-P80993, 1/10000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Rabbit IgG-HRP Secondary Antibody (HY-P8001, 1/10000) was used for 1 hour at room temperature.

  • NGF Antibody (YA3533) Product Image
    MedchemExpress Validation

    Immunohistochemical analysis of paraffin-embedded Mouse brain tissue using NGF Antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0) for 8 minutes. The tissues were blocked in QuickBlock for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HY-P81121, 1:100) in 4℃ overnight. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • NGF Antibody (YA3533) Product Image
    MedchemExpress Validation

    Immunohistochemical analysis of paraffin-embedded Mouse brain tissue using NGF Antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0) for 8 minutes. The tissues were blocked in QuickBlock for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HY-P81121, 1:100) in 4℃ overnight. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • NGF Antibody (YA3533) Product Image
    MedchemExpress Validation

    Immunocytochemistry analysis of SH-SY-5Y cells labeling NGF with NGF antibody (HY-P81121) at 1/50 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 for 10 minutes at room temperature, then blocked with QuickBlock™ Blocking Buffer for Immunol Staining for 10 min at room temperature. Cells were then incubated with NGF antibody (HY-P81121) at 1/50 dilution in QuickBlock™ Blocking Buffer for Immunol Staining at 4 ℃. Alexa Fluor® 488-conjugated AffiniPure Goat Anti-Rabbit IgG H&L（HY-P8002, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).

  • NGF Antibody (YA3533) Product Image
    MedchemExpress Validation

    Immunocytochemistry analysis of SH-SY-5Y cells labeling NGF with NGF antibody (HY-P81121) at 1/200 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 for 10 minutes at room temperature, then blocked with QuickBlock™ Blocking Buffer for Immunol Staining for 10 min at room temperature. Cells were then incubated with NGF antibody (HY-P81121) at 1/200 dilution in QuickBlock™ Blocking Buffer for Immunol Staining at 4 ℃. Alexa Fluor® 488-conjugated AffiniPure Goat Anti-Rabbit IgG H&L（HY-P8002, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).

  • NGF Antibody (YA3533) Product Image WB
  • NGF Antibody (YA3533) Product Image IHC-P
  • NGF Antibody (YA3533) Product Image IHC-P
  • NGF Antibody (YA3533) Product Image ICC
  • NGF Antibody (YA3533) Product Image ICC