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Vinculin Antibody (YA010)

(Synonyms: Vinculin, Metavinculin, MV, VCL)
2 Cited Publications
Customer Review

Based on 2 publication(s) in Google Scholar

Vinculin Antibody is a non-conjugated and Rabbit origined IgG monoclonal antibody, targeting to Vinculin. It can be used as a loading control antibody.

For research use only. We do not sell to patients.
  • Host:

    Rabbit

  • Isotype:

    IgG

  • Application:

    WB, IHC-P, ICC/IF

  • Reactivity :

    Human, Mouse, Rat

  • Formulation:

    Supplied in 1*TBS (pH7.4), 0.05% BSA and 40% Glycerol. Preservative: 0.05% Sodium Azide.

  • Conjugation:
    Non-conjugated
ALL WB IHC-P ICC
  • Experimental Validation Results for Vinculin Antibody (YA010)
  • Experimental Validation Results for Vinculin Antibody (YA010)
  • Experimental Validation Results for Vinculin Antibody (YA010)
  • Experimental Validation Results for Vinculin Antibody (YA010)
  • Experimental Validation Results for Vinculin Antibody (YA010)
  • Experimental Validation Results for Vinculin Antibody (YA010)
  • Experimental Validation Results for Vinculin Antibody (YA010)
  • Vinculin Antibody (YA010) Product Image
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To place orders, for customer services and technical support, please contact: MedChemExpress USA

Tel: 609-228-6898 E-mail: [email protected] [email protected]

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Publications Citing Use of MedChemExpress (MCE) Vinculin Antibody (YA010)

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2 Publications Citing Use of MCE Vinculin Antibody (YA010) (3)
IFWB
  • WB
  • WB
  • IF

Applications

Application
WB Info
WB: Western Blot
ICC/IF Info
ICC/IF: Immunocytochemistry/
Immunofluorescence
IP Info
IP: Immunoprecipitation
IHC-P Info
IHC-P: Immunohistochemistry-Paraffin
Dilution Ratio 1:500-1:2000 1:50-1:200 Use at an assay dependent concentration. 1:200

Product Details

Description

Vinculin Antibody is a non-conjugated and Rabbit origined IgG monoclonal antibody, targeting to Vinculin. It can be used as a loading control antibody.

  • Host Rabbit
  • Clonality Recombinant,Monoclonal
  • Species Reactivity
    Human, Mouse, Rat
  • Observed Molecular Weight
    Observed band size: 124 kDa Info
    Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.
  • Calculated Molecular Weight Predicted band size: 124 kDa
Species Reactivity Database
Immunogen

Synthetic peptide corresponding to Human Vinculin.AA range:1000-1060.

Sensitivity

Endogenous

Purification

Protein A affinity purified.

Conjugation

Non-conjugated

Modification

Unmodified

Isotype

IgG

RRID

AB_3102462

Product Properties

  • Appearance

    Solution

  • Formulation

    Supplied in 1*TBS (pH7.4), 0.05% BSA and 40% Glycerol. Preservative: 0.05% Sodium Azide.

  • Concentration

    Batch-dependent, Please check the COA for the concentration of each lot. Check Lot Concentration

  • Storage & Stability

    Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

  • Shipping

    Shipping with blue ice.

Verification Images

  • Experimental Validation Results for Vinculin Antibody (YA010)
    Western blot analysis of extracts from HeLa (lane 1(20μg)) 、HepG2 (lane 2(20μg)) 、NIH-3T3 (lane 3(20μg)) and C6 (lane 4(20μg)) using Vinculin Antibody (HY-P80372) . Proteins were transferred to a PVDF membrane and blocked with 5% nonfat dry milk in TBST for 1.5 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (β Tubulin, 1/10000) was used in 5% nonfat dry milk in TBST at 4℃ overnight. Goat Anti-Rabbit IgG-HRP Secondary Antibody (1/10,000) was used for 1 hour at room temperature.
  • Experimental Validation Results for Vinculin Antibody (YA010)
    Western blot analysis of extracts from WT Hela cells (lane 1(20μg)) and KD Hela cells (lane 2(20μg)) using Vinculin Antibody (HY-P80372) . Proteins were transferred to a PVDF membrane and blocked with 5% nonfat dry milk in TBST for 1.5 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Hsp90, 1/10000) was used in 5% nonfat dry milk in TBST at 4℃ overnight. Goat Anti-Rabbit IgG-HRP Secondary Antibody (1/10,000) was used for 1 hour at room temperature.
  • Experimental Validation Results for Vinculin Antibody (YA010)
    Western blot analysis of extracts from HepG2 (lane 2(20μg) , Hela(lane 3(20μg) and NIH3T3(lane 4(20μg) using Vinculin(HY-P80372) Rabbit mAb. Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P80438, 1/10000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Mouse IgG-HRP Secondary Antibody (1/10000) was used for 1 hour at room temperature.
  • Experimental Validation Results for Vinculin Antibody (YA010)
    Immunohistochemical analysis of paraffin-embedded Mouse testis tissue using Vinculin Antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 8 minutes. The tissues were blocked in QuickBlock for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HY-P80372, 1/1000) in 4℃ overnight. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
  • Experimental Validation Results for Vinculin Antibody (YA010)
    Immunohistochemical analysis of paraffin-embedded Mouse testis tissue using Vinculin Antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 8 minutes. The tissues were blocked in QuickBlock for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HY-P80372, 1/1000) in 4℃ overnight. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
  • Experimental Validation Results for Vinculin Antibody (YA010)
    Immunocytochemistry analysis of HUVEC cells labeling Vinculin with Vinculin Antibody (HY-P80372)at 1/50 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 for 10 minutes at room temperature, then blocked with QuickBlock™ Blocking Buffer for Immunol Staining for 10 min at room temperature. Cells were then incubated with Vinculin Antibody (HY-P80372) at 1/50 dilution in QuickBlock™ Blocking Buffer for Immunol Staining at 4 ℃. Alexa Fluor® 488-conjugated AffiniPure Goat Anti-Rabbit IgG H&L（HY-P8002, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).
  • Experimental Validation Results for Vinculin Antibody (YA010)
    Immunocytochemistry analysis of Hela cells labeling Vinculin with Vinculin Antibody (HY-P80372) at 1/50 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 for 10 minutes at room temperature, then blocked with QuickBlock™ Blocking Buffer for Immunol Staining for 10 min at room temperature. Cells were then incubated with Vinculin Antibody (HY-P80372) at 1/50 dilution in QuickBlock™ Blocking Buffer for Immunol Staining at 4 ℃. Alexa Fluor® 488-conjugated AffiniPure Goat Anti-Rabbit IgG H&L（HY-P8002, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).

Background

  • Function

    Actin filament (F-actin)-binding protein involved in cell-matrix adhesion and cell-cell adhesion. Regulates cell-surface E-cadherin expression and potentiates mechanosensing by the E-cadherin complex. May also play important roles in cell morphology and locomotion

  • Subcellular Localization

    Cell membrane; Peripheral membrane protein; Cytoplasmic side; Cell junction, adherens junction; Cell junction, focal adhesion; Cytoplasm, cytoskeleton; Cell membrane, sarcolemma; Peripheral membrane protein; Cytoplasmic side; Cell projection, podosome

  • Expression


    Tissue_specificity:Metavinculin is a muscle-specific protein.

  • Subunit

    Exhibits self-association properties. Part of a complex composed of THSD1, PTK2/FAK1, TLN1 and VCL (PubMed:29069646).

  • SwissProt ID

    P18206

  • Gene ID
    7414 [NCBI]
  • Synonyms

    Vinculin, Metavinculin, MV, VCL

  • Research Field

    Signal Transduction

Vinculin Antibody (YA010) Related Classifications

MOQ
Minimum order quantity
100 mg

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Measured by its ability to inhibit anti-CD3-induced proliferation of stimulated CTLL-2 mouse cytotoxic T cells. The ED50 this effect is 0.8023 μg/ml, corresponding to a specific activity is 1246.42 units/mg.

Assay Types
  • 1/16

    MedchemExpress Validation
     Western blot analysis of extracts from HeLa (lane 1(20μg)) 、HepG2 (lane 2(20μg)) 、NIH-3T3 (lane 3(20μg)) and C6 (lane 4(20μg)) using Vinculin Antibody (HY-P80372) . Proteins were transferred to a PVDF membrane and blocked with 5% nonfat dry milk in TBST for 1.5 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (β Tubulin, 1/10000) was used in 5% nonfat dry milk in TBST at 4℃ overnight. Goat Anti-Rabbit IgG-HRP Secondary Antibody (1/10,000) was used for 1 hour at room temperature.

  • 1/16

    MedchemExpress Validation
     Western blot analysis of extracts from WT Hela cells (lane 1(20μg)) and KD Hela cells (lane 2(20μg)) using Vinculin Antibody (HY-P80372) . Proteins were transferred to a PVDF membrane and blocked with 5% nonfat dry milk in TBST for 1.5 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Hsp90, 1/10000) was used in 5% nonfat dry milk in TBST at 4℃ overnight. Goat Anti-Rabbit IgG-HRP Secondary Antibody (1/10,000) was used for 1 hour at room temperature.

  • 1/16

    MedChemExpress Validation
     Western blot analysis of extracts from HepG2 (lane 2(20μg) , Hela(lane 3(20μg) and NIH3T3(lane 4(20μg) using Vinculin(HY-P80372) Rabbit mAb. Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P80438, 1/10000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Mouse IgG-HRP Secondary Antibody (1/10000) was used for 1 hour at room temperature.

  • 1/16

    MedchemExpress Validation
     Immunohistochemical analysis of paraffin-embedded Mouse testis tissue using Vinculin Antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 8 minutes. The tissues were blocked in QuickBlock for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HY-P80372, 1/1000) in 4℃ overnight. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • 1/16

    MedchemExpress Validation
     Immunohistochemical analysis of paraffin-embedded Mouse testis tissue using Vinculin Antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 8 minutes. The tissues were blocked in QuickBlock for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HY-P80372, 1/1000) in 4℃ overnight. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • 1/16

    MedchemExpress Validation
     Immunocytochemistry analysis of HUVEC cells labeling Vinculin with Vinculin Antibody (HY-P80372)at 1/50 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 for 10 minutes at room temperature, then blocked with QuickBlock™ Blocking Buffer for Immunol Staining for 10 min at room temperature. Cells were then incubated with Vinculin Antibody (HY-P80372) at 1/50 dilution in QuickBlock™ Blocking Buffer for Immunol Staining at 4 ℃. Alexa Fluor® 488-conjugated AffiniPure Goat Anti-Rabbit IgG H&L（HY-P8002, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).

  • 1/16

    MedchemExpress Validation
     Immunocytochemistry analysis of Hela cells labeling Vinculin with Vinculin Antibody (HY-P80372) at 1/50 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 for 10 minutes at room temperature, then blocked with QuickBlock™ Blocking Buffer for Immunol Staining for 10 min at room temperature. Cells were then incubated with Vinculin Antibody (HY-P80372) at 1/50 dilution in QuickBlock™ Blocking Buffer for Immunol Staining at 4 ℃. Alexa Fluor® 488-conjugated AffiniPure Goat Anti-Rabbit IgG H&L（HY-P8002, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).

  • 1/16

    Vinculin Antibody (YA010) purchased from MedChemExpress. Usage Cited in: FASEB J. 2026 May 31;40(10):e71921. [Abstract]

    Customer Validation
     Overexpression of PP2AC increased PJNK levels in 293 T cells. Antibodies used for WB were as follows: rabbit anti-PP2AC (1:1000), rabbit anti-HA (1:1000), rabbit anti-Vinculin (Vinculin Antibody (YA010), 1:1000, HY-P80372), and rabbit anti-pJNK (1:1000), rabbit anti-JNK (1:1000).

  • 1/16

    Vinculin Antibody (YA010) purchased from MedChemExpress. Usage Cited in: FASEB J. 2026 May 31;40(10):e71921. [Abstract]

    Customer Validation
     Knockdown of PP2AC decreased the RhoA protein level in PANC-1 cells. Antibodies used for WB were as follows: rabbit anti-PP2AC (1:1000), rabbit anti-Vinculin (Vinculin Antibody (YA010), 1:1000, HY-P80372), and rabbit anti-RhoA (1:1000).

  • 1/16

    Vinculin Antibody (YA010) purchased from MedChemExpress. Usage Cited in: Adv Fiber Mater. 2025 Apr 24.

    Customer Validation
     Fluorescence staining of vinculin in NIH/3T3 cells after treatment with Ti, Ti + US, Ti/PiG, and Ti/PiG + US. Scale bar = 200 μm.

  • Vinculin Antibody (YA010) Product Image
    1/16
  • Experimental Validation Results for Vinculin Antibody (YA010)
  • Experimental Validation Results for Vinculin Antibody (YA010)
  • Experimental Validation Results for Vinculin Antibody (YA010)
  • Experimental Validation Results for Vinculin Antibody (YA010)
  • Experimental Validation Results for Vinculin Antibody (YA010)
  • Experimental Validation Results for Vinculin Antibody (YA010)
  • Experimental Validation Results for Vinculin Antibody (YA010)
  • Experimental Validation Results for Vinculin Antibody (YA010)
  • Experimental Validation Results for Vinculin Antibody (YA010)
  • Experimental Validation Results for Vinculin Antibody (YA010)
  • Vinculin Antibody (YA010) Product Image
  • Experimental Validation Results for Vinculin Antibody (YA010)
    1/1
    MedchemExpress Validation
     Western blot analysis of extracts from HeLa (lane 1(20μg)) 、HepG2 (lane 2(20μg)) 、NIH-3T3 (lane 3(20μg)) and C6 (lane 4(20μg)) using Vinculin Antibody (HY-P80372) . Proteins were transferred to a PVDF membrane and blocked with 5% nonfat dry milk in TBST for 1.5 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (β Tubulin, 1/10000) was used in 5% nonfat dry milk in TBST at 4℃ overnight. Goat Anti-Rabbit IgG-HRP Secondary Antibody (1/10,000) was used for 1 hour at room temperature.
  • Experimental Validation Results for Vinculin Antibody (YA010)
    1/1
    MedchemExpress Validation
     Western blot analysis of extracts from WT Hela cells (lane 1(20μg)) and KD Hela cells (lane 2(20μg)) using Vinculin Antibody (HY-P80372) . Proteins were transferred to a PVDF membrane and blocked with 5% nonfat dry milk in TBST for 1.5 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Hsp90, 1/10000) was used in 5% nonfat dry milk in TBST at 4℃ overnight. Goat Anti-Rabbit IgG-HRP Secondary Antibody (1/10,000) was used for 1 hour at room temperature.
  • Experimental Validation Results for Vinculin Antibody (YA010)
    1/1
    MedChemExpress Validation
     Western blot analysis of extracts from HepG2 (lane 2(20μg) , Hela(lane 3(20μg) and NIH3T3(lane 4(20μg) using Vinculin(HY-P80372) Rabbit mAb. Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P80438, 1/10000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Mouse IgG-HRP Secondary Antibody (1/10000) was used for 1 hour at room temperature.
  • Experimental Validation Results for Vinculin Antibody (YA010)
    1/1
    MedchemExpress Validation
     Immunohistochemical analysis of paraffin-embedded Mouse testis tissue using Vinculin Antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 8 minutes. The tissues were blocked in QuickBlock for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HY-P80372, 1/1000) in 4℃ overnight. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
  • Experimental Validation Results for Vinculin Antibody (YA010)
    1/1
    MedchemExpress Validation
     Immunohistochemical analysis of paraffin-embedded Mouse testis tissue using Vinculin Antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 8 minutes. The tissues were blocked in QuickBlock for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HY-P80372, 1/1000) in 4℃ overnight. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
  • Experimental Validation Results for Vinculin Antibody (YA010)
    1/1
    MedchemExpress Validation
     Immunocytochemistry analysis of HUVEC cells labeling Vinculin with Vinculin Antibody (HY-P80372)at 1/50 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 for 10 minutes at room temperature, then blocked with QuickBlock™ Blocking Buffer for Immunol Staining for 10 min at room temperature. Cells were then incubated with Vinculin Antibody (HY-P80372) at 1/50 dilution in QuickBlock™ Blocking Buffer for Immunol Staining at 4 ℃. Alexa Fluor® 488-conjugated AffiniPure Goat Anti-Rabbit IgG H&L（HY-P8002, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).
  • Experimental Validation Results for Vinculin Antibody (YA010)
    1/1
    MedchemExpress Validation
     Immunocytochemistry analysis of Hela cells labeling Vinculin with Vinculin Antibody (HY-P80372) at 1/50 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 for 10 minutes at room temperature, then blocked with QuickBlock™ Blocking Buffer for Immunol Staining for 10 min at room temperature. Cells were then incubated with Vinculin Antibody (HY-P80372) at 1/50 dilution in QuickBlock™ Blocking Buffer for Immunol Staining at 4 ℃. Alexa Fluor® 488-conjugated AffiniPure Goat Anti-Rabbit IgG H&L（HY-P8002, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).
  • Experimental Validation Results for Vinculin Antibody (YA010)
  • Experimental Validation Results for Vinculin Antibody (YA010)
  • Experimental Validation Results for Vinculin Antibody (YA010)
  • Experimental Validation Results for Vinculin Antibody (YA010)
  • Experimental Validation Results for Vinculin Antibody (YA010)
  • Experimental Validation Results for Vinculin Antibody (YA010)
  • Experimental Validation Results for Vinculin Antibody (YA010)

  • Vinculin Antibody (YA010) purchased from MedChemExpress. Usage Cited in: FASEB J. 2026 May 31;40(10):e71921. [Abstract]

    Overexpression of PP2AC increased PJNK levels in 293 T cells. Antibodies used for WB were as follows: rabbit anti-PP2AC (1:1000), rabbit anti-HA (1:1000), rabbit anti-Vinculin (Vinculin Antibody (YA010), 1:1000, HY-P80372), and rabbit anti-pJNK (1:1000), rabbit anti-JNK (1:1000).

  • Vinculin Antibody (YA010) purchased from MedChemExpress. Usage Cited in: FASEB J. 2026 May 31;40(10):e71921. [Abstract]

    Knockdown of PP2AC decreased the RhoA protein level in PANC-1 cells. Antibodies used for WB were as follows: rabbit anti-PP2AC (1:1000), rabbit anti-Vinculin (Vinculin Antibody (YA010), 1:1000, HY-P80372), and rabbit anti-RhoA (1:1000).

  • Vinculin Antibody (YA010) purchased from MedChemExpress. Usage Cited in: Adv Fiber Mater. 2025 Apr 24.

    Fluorescence staining of vinculin in NIH/3T3 cells after treatment with Ti, Ti + US, Ti/PiG, and Ti/PiG + US. Scale bar = 200 μm.

  • Vinculin Antibody (YA010) Product Image
    MedchemExpress Validation

    Western blot analysis of extracts from HeLa (lane 1(20μg)) 、HepG2 (lane 2(20μg)) 、NIH-3T3 (lane 3(20μg)) and C6 (lane 4(20μg)) using Vinculin Antibody (HY-P80372) . Proteins were transferred to a PVDF membrane and blocked with 5% nonfat dry milk in TBST for 1.5 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (β Tubulin, 1/10000) was used in 5% nonfat dry milk in TBST at 4℃ overnight. Goat Anti-Rabbit IgG-HRP Secondary Antibody (1/10,000) was used for 1 hour at room temperature.

  • Vinculin Antibody (YA010) Product Image
    MedchemExpress Validation

    Western blot analysis of extracts from WT Hela cells (lane 1(20μg)) and KD Hela cells (lane 2(20μg)) using Vinculin Antibody (HY-P80372) . Proteins were transferred to a PVDF membrane and blocked with 5% nonfat dry milk in TBST for 1.5 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Hsp90, 1/10000) was used in 5% nonfat dry milk in TBST at 4℃ overnight. Goat Anti-Rabbit IgG-HRP Secondary Antibody (1/10,000) was used for 1 hour at room temperature.

  • Vinculin Antibody (YA010) Product Image
    MedChemExpress Validation

    Western blot analysis of extracts from HepG2 (lane 2(20μg) , Hela(lane 3(20μg) and NIH3T3(lane 4(20μg) using Vinculin(HY-P80372) Rabbit mAb. Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P80438, 1/10000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Mouse IgG-HRP Secondary Antibody (1/10000) was used for 1 hour at room temperature.

  • Vinculin Antibody (YA010) Product Image
    MedchemExpress Validation

    Immunohistochemical analysis of paraffin-embedded Mouse testis tissue using Vinculin Antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 8 minutes. The tissues were blocked in QuickBlock for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HY-P80372, 1/1000) in 4℃ overnight. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • Vinculin Antibody (YA010) Product Image
    MedchemExpress Validation

    Immunohistochemical analysis of paraffin-embedded Mouse testis tissue using Vinculin Antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 8 minutes. The tissues were blocked in QuickBlock for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HY-P80372, 1/1000) in 4℃ overnight. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • Vinculin Antibody (YA010) Product Image
    MedchemExpress Validation

    Immunocytochemistry analysis of HUVEC cells labeling Vinculin with Vinculin Antibody (HY-P80372)at 1/50 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 for 10 minutes at room temperature, then blocked with QuickBlock™ Blocking Buffer for Immunol Staining for 10 min at room temperature. Cells were then incubated with Vinculin Antibody (HY-P80372) at 1/50 dilution in QuickBlock™ Blocking Buffer for Immunol Staining at 4 ℃. Alexa Fluor® 488-conjugated AffiniPure Goat Anti-Rabbit IgG H&L（HY-P8002, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).

  • Vinculin Antibody (YA010) Product Image
    MedchemExpress Validation

    Immunocytochemistry analysis of Hela cells labeling Vinculin with Vinculin Antibody (HY-P80372) at 1/50 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 for 10 minutes at room temperature, then blocked with QuickBlock™ Blocking Buffer for Immunol Staining for 10 min at room temperature. Cells were then incubated with Vinculin Antibody (HY-P80372) at 1/50 dilution in QuickBlock™ Blocking Buffer for Immunol Staining at 4 ℃. Alexa Fluor® 488-conjugated AffiniPure Goat Anti-Rabbit IgG H&L（HY-P8002, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).

  • Vinculin Antibody (YA010) Product Image WB
  • Vinculin Antibody (YA010) Product Image WB
  • Vinculin Antibody (YA010) Product Image WB
  • Vinculin Antibody (YA010) Product Image IHC-P
  • Vinculin Antibody (YA010) Product Image IHC-P
  • Vinculin Antibody (YA010) Product Image ICC
  • Vinculin Antibody (YA010) Product Image ICC