G3BP Antibody (YA5543)
(Synonyms: Ras GTPase-activating protein-binding protein 1; G3BP-1; EC 3.6.4.13; ATP-dependent DNA helicase VIII; hDH VIII; GAP SH3 domain-binding protein 1; )
Based on 1 Customer Validation
G3BP Antibody (YA5543) is a Mouse-derived and non-conjugated IgG2b monoclonal antibody, targeting to G3BP.
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Host:
Mouse
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Isotype:
IgG
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Application:
WB, ICC/IF, ELISA
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Reactivity :
Human, Mouse, Rat
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Formulation:
Supplied in PBS, 50% glycerol, 0.05% Proclin 300, 0.05%BSA
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Conjugation:
Non-conjugated
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
Applications
| Application |
WB
WB: Western Blot
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ICC/IF
ICC/IF: Immunocytochemistry/
Immunofluorescence |
ELISA
ELISA: Enzyme Linked Immunosorbent Assay
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|---|---|---|---|
| Dilution Ratio | 1:500-2000 | 1:100-500 | 1:1000-5000 |
Product Details
G3BP Antibody (YA5543) is a Mouse-derived and non-conjugated IgG2b monoclonal antibody, targeting to G3BP.
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Host Mouse
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Clonality Monoclonal
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Species ReactivityHuman, Mouse, Rat
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Observed Molecular WeightObserved band size: 68 kDa
Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.
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Calculated Molecular Weight Predicted band size: 52 kDa
Synthesized peptide derived from human protein. AA range: 200-300
Protein G
Non-conjugated
Unmodified
IgG
Product Properties
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Appearance
Solution
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Formulation
Supplied in PBS, 50% glycerol, 0.05% Proclin 300, 0.05%BSA
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Concentration
Batch-dependent, Please check the COA for the concentration of each lot. Check Lot Concentration
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Storage & Stability
Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.
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Shipping
Shipping with blue ice.
Background
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Function
G3BP is a Protein involved in various processes, such as stress granule formation and innate immunity. Plays an essential role in stress granule formation. Stress granules are membraneless compartments that store mRNAs and proteins, such as stalled translation pre-initiation complexes, in response to stress. Promotes formation of stress granules phase-separated membraneless compartment by undergoing liquid-liquid phase separation (LLPS) upon unfolded RNA-binding: functions as a molecular switch that triggers RNA-dependent LLPS in response to a rise in intracellular free RNA concentrations. Also acts as an ATP- and magnesium-dependent helicase: unwinds DNA/DNA, RNA/DNA, and RNA/RNA substrates with comparable efficiency. Acts unidirectionally by moving in the 5' to 3' direction along the bound single-stranded DNA. Unwinds preferentially partial DNA and RNA duplexes having a 17 bp annealed portion and either a hanging 3' tail or hanging tails at both 5'- and 3'-ends. Plays an essential role in innate immunity by promoting CGAS and RIGI activity. Participates in the DNA-triggered cGAS/STING pathway by promoting the DNA binding and activation of CGAS. Triggers the condensation of cGAS, a process probably linked to the formation of membrane-less organelles. Also enhances RIGI-induced type I interferon production probably by helping RIGI at sensing pathogenic RNA. May also act as a phosphorylation-dependent sequence-specific endoribonuclease in vitro: Cleaves exclusively between cytosine and adenine and cleaves MYC mRNA preferentially at the 3'-UTR[1][2][3][4][5][6][7][8][9][10][11][12][13][14][15][16][17][18].
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Subcellular Localization
Cytoplasm, cytosol; Perikaryon; Cytoplasm, Stress granule; Nucleus
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Expression
Tissue_specificity:general expression -
Isoforms & Post-Translational Modification
Q13283 has 2 isomers: Q13283-1: 52164 Da (predicted); Q13283-2: 13874 Da (predicted).
Phosphorylation of the acidic disordered region regulates stress granule assembly (PubMed:32302571, PubMed:32302572). RASA1-dependent phosphorylation of Ser-149 induces a conformational change that prevents self-association (PubMed:11604510, PubMed:12642610). Dephosphorylation after HRAS activation is required for stress granule assembly (PubMed:11604510, PubMed:12642610). Ser-149 phosphorylation induces partial nuclear localization (PubMed:11604510);Ubiquitinated by TRIM21 via 'Lys-63'-linked polyubiquitination in the NTF2 domain in response to heat shock, leading to stress granule disassembly: ubiquitination promotes interaction with the FAF2 adapter, followed by interaction with VCP, which extracts G3BP1 from stress granules, leading to stress granule disassembly (PubMed:34739333, PubMed:36692217). In case of prolonged stress, ubiquitination by TRIM21 leads to autophagy-dependent degradation of G3BP1 via recruitment of ubiquitinated G3BP1 by SQSTM1 and/or CALCOCO2 to autophagosomes (PubMed:34739333, PubMed:36692217);(Microbial infection) Cleaved by human enterovirus 71; this cleavage induces the disassembly of cytoplasmic stress granules (PubMed:30006004). Cleaved by Foot-and-mouth disease virus; this cleavage suppresses the formation of cytoplasmic stress granules (PubMed:30404792);Arg-435 is dimethylated, probably to asymmetric dimethylarginine;(Microbial infection) Cleaved by Encephalomyocarditis virus protease 3C; this cleavage suppresses the formation of cytoplasmic stress granules -
Subunit
Homodimer and oligomer (PubMed:12642610, PubMed:24324649). Interacts (via NTF2 domain) with USP10; inhibiting stress granule formation by lowering G3BP1 valence (PubMed:11439350, PubMed:23279204, PubMed:27022092, PubMed:31981475, PubMed:32302570, PubMed:36183834, PubMed:36279435).
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SwissProt ID
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Synonyms
Ras GTPase-activating protein-binding protein 1; G3BP-1; EC 3.6.4.13; ATP-dependent DNA helicase VIII; hDH VIII; GAP SH3 domain-binding protein 1;
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