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CRBN Antibody (YA6516)

(Synonyms: 2610203G15Rik antibody; 2900045O07Rik antibody; AF229032 antibody; AW108261 antibody; Cereblon antibody; Crbn antibody; CRBN_HUMAN antibody; DKFZp781K0715 antibody; MGC27358 antibody; MRT2A antibody; 2610203G15Rik antibody; 2900045O07Rik antibody; AF229032 antibody; AW108261 antibody; Cereblon antibody; Crbn antibody; CRBN_HUMAN antibody; DKFZp781K0715 antibody; MGC27358 antibody; MRT2A antibody; OTTHUMP00000209555 antibody; piL antibody; Protein cereblon antibody; Protein x 0001 antibody; )
Customer Review

Based on 1 Customer Validation

CRBN Antibody (YA6516) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to CRBN.

For research use only. We do not sell to patients.
  • Host:

    Rabbit

  • Isotype:

    IgG

  • Application:

    WB, IHC-P, IP

  • Reactivity :

    Human, Mouse, Rat

  • Formulation:

    Supplied in PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

  • Conjugation:
    Non-conjugated
ALL WB IHC-P mIHC
  • Experimental Validation Results for CRBN Antibody (YA6516)
  • Experimental Validation Results for CRBN Antibody (YA6516)
  • Experimental Validation Results for CRBN Antibody (YA6516)
  • Experimental Validation Results for CRBN Antibody (YA6516)
  • Experimental Validation Results for CRBN Antibody (YA6516)
  • CRBN Antibody (YA6516) Product Image
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Tel: 609-228-6898 E-mail: [email protected] [email protected]

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Applications

Application
WB Info
WB: Western Blot
IHC-P Info
IHC-P: Immunohistochemistry-Paraffin
IP Info
IP: Immunoprecipitation
Dilution Ratio 1:1000 1:200 1-2μg/sample

Product Details

Description

CRBN Antibody (YA6516) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to CRBN.

  • Host Rabbit
  • Clonality Recombinant,Monoclonal
  • Species Reactivity
    Human, Mouse, Rat
  • Observed Molecular Weight
    Observed band size: 51 kDa Info
    Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.
  • Calculated Molecular Weight Predicted band size: 51 kDa
Immunogen

Recombinant protein within human CRBN aa 1-442 / 442.

Purification

affinity purified.

Conjugation

Non-conjugated

Modification

Unmodified

Isotype

IgG

RRID

AB_3719219

Product Properties

  • Appearance

    Liquid

  • Formulation

    Supplied in PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

  • Concentration

    Batch-dependent, Please check the COA for the concentration of each lot. Check Lot Concentration

  • Storage & Stability

    Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

  • Shipping

    Shipping with blue ice.

Verification Images

  • Experimental Validation Results for CRBN Antibody (YA6516)
    Western blot analysis of extracts from HEK293T (lane2(20μg), Hela (lane3(20μg), SH-SY5Y (lane4(20μg) and NIH3T3 (lane5(20μg) using CRBN Antibody (HY-P86823). Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P80993, 1/10,000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Rabbit IgG-HRP Secondary Antibody (HY-P8001 ,1/10,000) was used for 1 hour at room temperature.
  • Experimental Validation Results for CRBN Antibody (YA6516)
    Immunohistochemical analysis of paraffin-embedded Mouse Brain tissue using CRBN antibody was performed. The section was pretreated using high-temperature and high-pressure mediated EDTA antigen retrieval buffer (pH 9.0), for 5 minutes. The tissues were incubated with primary antibody (HY-P86823, 1:200 dilution) at room temperature for 60 minutes. Detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. The tissues were counterstained with hematoxylin and mounted with neutral balsam mounting medium.
  • Experimental Validation Results for CRBN Antibody (YA6516)
    Immunohistochemical analysis of paraffin-embedded Mouse Brain tissue using CRBN antibody was performed. The section was pretreated using high-temperature and high-pressure mediated EDTA antigen retrieval buffer (pH 9.0), for 5 minutes. The tissues were incubated with primary antibody (HY-P86823, 1:200 dilution) at room temperature for 60 minutes. Detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. The tissues were counterstained with hematoxylin and mounted with neutral balsam mounting medium.
  • Experimental Validation Results for CRBN Antibody (YA6516)
    Tyramide signaling amplification based immunofluorescence was performed on paraffin-embedded Mouse Brain tissue using CRBN antibody. Antigen retrieval was performed in EDTA buffer pH 9.0 (95 °C, 20 min) followed by cooling to RT. Then incubated with primary antibody (HY-P86823, 1:300 dilution) at room temperature for 60 minutes and HRP conjugated secondary antibody for 10 minutes. Fluorescence was then developed with Vari Fluor 532 TSA (200×)（HY-D1832）. The tissues were counterstained with DAPI and mounted with Anti-fade mounting medium.
  • Experimental Validation Results for CRBN Antibody (YA6516)
    Tyramide signaling amplification based immunofluorescence was performed on paraffin-embedded Mouse Brain tissue using CRBN antibody. Antigen retrieval was performed in EDTA buffer pH 9.0 (95 °C, 20 min) followed by cooling to RT. Then incubated with primary antibody (HY-P86823, 1:300 dilution) at room temperature for 60 minutes and HRP conjugated secondary antibody for 10 minutes. Fluorescence was then developed with Vari Fluor 532 TSA (200×)（HY-D1832）. The tissues were counterstained with DAPI and mounted with Anti-fade mounting medium.

Background

  • Function

    CRBN is a Substrate recognition component of a DCX (DDB1-CUL4-X-box) E3 protein ligase complex that mediates the ubiquitination and subsequent proteasomal degradation of target proteins, such as MEIS2, ILF2 or GLUL. Normal degradation of key regulatory proteins is required for normal limb outgrowth and expression of the fibroblast growth factor FGF8. Maintains presynaptic glutamate release and consequently cognitive functions, such as memory and learning, by negatively regulating large-conductance calcium-activated potassium (BK) channels in excitatory neurons. Likely to function by regulating the assembly and neuronal surface expression of BK channels via its interaction with KCNT1. May also be involved in regulating anxiety-like behaviors via a BK channel-independent mechanism. Plays a negative role in TLR4 signaling by interacting with TRAF6 and ECSIT, leading to inhibition of ECSIT ubiquitination, an important step of the signaling[1][2][3][4][5][6][7][8][9].

  • Subcellular Localization

    Cytoplasm,Nucleus,Membrane

  • Expression


    Tissue_Specificity: Widely expressed. Highly expressed in brain

  • Isoforms & Post-Translational Modification

    Q96SW2 has two isomers: Q96SW2-1: 50546 Da (predicted); Q96SW2-2: 50475 Da (predicted).
    Ubiquitinated, ubiquitination is mediated by its own DCX protein ligase complex

  • Subunit

    Interacts with KCNT1 (By similarity)

  • SwissProt ID

    Q96SW2

  • Gene ID
    51185 [NCBI]
  • Synonyms

    2610203G15Rik antibody; 2900045O07Rik antibody; AF229032 antibody; AW108261 antibody; Cereblon antibody; Crbn antibody; CRBN_HUMAN antibody; DKFZp781K0715 antibody; MGC27358 antibody; MRT2A antibody; 2610203G15Rik antibody; 2900045O07Rik antibody; AF229032 antibody; AW108261 antibody; Cereblon antibody; Crbn antibody; CRBN_HUMAN antibody; DKFZp781K0715 antibody; MGC27358 antibody; MRT2A antibody; OTTHUMP00000209555 antibody; piL antibody; Protein cereblon antibody; Protein x 0001 antibody;

CRBN Antibody (YA6516) Related Classifications

MOQ
Minimum order quantity
100 mg

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Measured by its ability to inhibit anti-CD3-induced proliferation of stimulated CTLL-2 mouse cytotoxic T cells. The ED50 this effect is 0.8023 μg/ml, corresponding to a specific activity is 1246.42 units/mg.

Assay Types
  • 1/16

    MedChemExpress Validation
     Western blot analysis of extracts from HEK293T (lane2(20μg), Hela (lane3(20μg), SH-SY5Y (lane4(20μg) and NIH3T3 (lane5(20μg) using CRBN Antibody (HY-P86823). Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P80993, 1/10,000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Rabbit IgG-HRP Secondary Antibody (HY-P8001 ,1/10,000) was used for 1 hour at room temperature.

  • 1/16

    MedchemExpress Validation
     Immunohistochemical analysis of paraffin-embedded Mouse Brain tissue using CRBN antibody was performed. The section was pretreated using high-temperature and high-pressure mediated EDTA antigen retrieval buffer (pH 9.0), for 5 minutes. The tissues were incubated with primary antibody (HY-P86823, 1:200 dilution) at room temperature for 60 minutes. Detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. The tissues were counterstained with hematoxylin and mounted with neutral balsam mounting medium.

  • 1/16

    MedchemExpress Validation
     Immunohistochemical analysis of paraffin-embedded Mouse Brain tissue using CRBN antibody was performed. The section was pretreated using high-temperature and high-pressure mediated EDTA antigen retrieval buffer (pH 9.0), for 5 minutes. The tissues were incubated with primary antibody (HY-P86823, 1:200 dilution) at room temperature for 60 minutes. Detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. The tissues were counterstained with hematoxylin and mounted with neutral balsam mounting medium.

  • 1/16

    MedchemExpress Validation
     Tyramide signaling amplification based immunofluorescence was performed on paraffin-embedded Mouse Brain tissue using CRBN antibody. Antigen retrieval was performed in EDTA buffer pH 9.0 (95 °C, 20 min) followed by cooling to RT. Then incubated with primary antibody (HY-P86823, 1:300 dilution) at room temperature for 60 minutes and HRP conjugated secondary antibody for 10 minutes. Fluorescence was then developed with Vari Fluor 532 TSA (200×)（HY-D1832）. The tissues were counterstained with DAPI and mounted with Anti-fade mounting medium.

  • 1/16

    MedchemExpress Validation
     Tyramide signaling amplification based immunofluorescence was performed on paraffin-embedded Mouse Brain tissue using CRBN antibody. Antigen retrieval was performed in EDTA buffer pH 9.0 (95 °C, 20 min) followed by cooling to RT. Then incubated with primary antibody (HY-P86823, 1:300 dilution) at room temperature for 60 minutes and HRP conjugated secondary antibody for 10 minutes. Fluorescence was then developed with Vari Fluor 532 TSA (200×)（HY-D1832）. The tissues were counterstained with DAPI and mounted with Anti-fade mounting medium.

  • CRBN Antibody (YA6516) Product Image
    1/16
  • Experimental Validation Results for CRBN Antibody (YA6516)
  • Experimental Validation Results for CRBN Antibody (YA6516)
  • Experimental Validation Results for CRBN Antibody (YA6516)
  • Experimental Validation Results for CRBN Antibody (YA6516)
  • Experimental Validation Results for CRBN Antibody (YA6516)
  • CRBN Antibody (YA6516) Product Image
  • Experimental Validation Results for CRBN Antibody (YA6516)
    1/1
    MedChemExpress Validation
     Western blot analysis of extracts from HEK293T (lane2(20μg), Hela (lane3(20μg), SH-SY5Y (lane4(20μg) and NIH3T3 (lane5(20μg) using CRBN Antibody (HY-P86823). Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P80993, 1/10,000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Rabbit IgG-HRP Secondary Antibody (HY-P8001 ,1/10,000) was used for 1 hour at room temperature.
  • Experimental Validation Results for CRBN Antibody (YA6516)
    1/1
    MedchemExpress Validation
     Immunohistochemical analysis of paraffin-embedded Mouse Brain tissue using CRBN antibody was performed. The section was pretreated using high-temperature and high-pressure mediated EDTA antigen retrieval buffer (pH 9.0), for 5 minutes. The tissues were incubated with primary antibody (HY-P86823, 1:200 dilution) at room temperature for 60 minutes. Detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. The tissues were counterstained with hematoxylin and mounted with neutral balsam mounting medium.
  • Experimental Validation Results for CRBN Antibody (YA6516)
    1/1
    MedchemExpress Validation
     Immunohistochemical analysis of paraffin-embedded Mouse Brain tissue using CRBN antibody was performed. The section was pretreated using high-temperature and high-pressure mediated EDTA antigen retrieval buffer (pH 9.0), for 5 minutes. The tissues were incubated with primary antibody (HY-P86823, 1:200 dilution) at room temperature for 60 minutes. Detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. The tissues were counterstained with hematoxylin and mounted with neutral balsam mounting medium.
  • Experimental Validation Results for CRBN Antibody (YA6516)
    1/1
    MedchemExpress Validation
     Tyramide signaling amplification based immunofluorescence was performed on paraffin-embedded Mouse Brain tissue using CRBN antibody. Antigen retrieval was performed in EDTA buffer pH 9.0 (95 °C, 20 min) followed by cooling to RT. Then incubated with primary antibody (HY-P86823, 1:300 dilution) at room temperature for 60 minutes and HRP conjugated secondary antibody for 10 minutes. Fluorescence was then developed with Vari Fluor 532 TSA (200×)（HY-D1832）. The tissues were counterstained with DAPI and mounted with Anti-fade mounting medium.
  • Experimental Validation Results for CRBN Antibody (YA6516)
    1/1
    MedchemExpress Validation
     Tyramide signaling amplification based immunofluorescence was performed on paraffin-embedded Mouse Brain tissue using CRBN antibody. Antigen retrieval was performed in EDTA buffer pH 9.0 (95 °C, 20 min) followed by cooling to RT. Then incubated with primary antibody (HY-P86823, 1:300 dilution) at room temperature for 60 minutes and HRP conjugated secondary antibody for 10 minutes. Fluorescence was then developed with Vari Fluor 532 TSA (200×)（HY-D1832）. The tissues were counterstained with DAPI and mounted with Anti-fade mounting medium.
  • Experimental Validation Results for CRBN Antibody (YA6516)
  • Experimental Validation Results for CRBN Antibody (YA6516)
  • Experimental Validation Results for CRBN Antibody (YA6516)
  • Experimental Validation Results for CRBN Antibody (YA6516)
  • Experimental Validation Results for CRBN Antibody (YA6516)
  • CRBN Antibody (YA6516) Product Image
    MedChemExpress Validation

    Western blot analysis of extracts from HEK293T (lane2(20μg), Hela (lane3(20μg), SH-SY5Y (lane4(20μg) and NIH3T3 (lane5(20μg) using CRBN Antibody (HY-P86823). Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P80993, 1/10,000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Rabbit IgG-HRP Secondary Antibody (HY-P8001 ,1/10,000) was used for 1 hour at room temperature.

  • CRBN Antibody (YA6516) Product Image
    MedchemExpress Validation

    Immunohistochemical analysis of paraffin-embedded Mouse Brain tissue using CRBN antibody was performed. The section was pretreated using high-temperature and high-pressure mediated EDTA antigen retrieval buffer (pH 9.0), for 5 minutes. The tissues were incubated with primary antibody (HY-P86823, 1:200 dilution) at room temperature for 60 minutes. Detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. The tissues were counterstained with hematoxylin and mounted with neutral balsam mounting medium.

  • CRBN Antibody (YA6516) Product Image
    MedchemExpress Validation

    Immunohistochemical analysis of paraffin-embedded Mouse Brain tissue using CRBN antibody was performed. The section was pretreated using high-temperature and high-pressure mediated EDTA antigen retrieval buffer (pH 9.0), for 5 minutes. The tissues were incubated with primary antibody (HY-P86823, 1:200 dilution) at room temperature for 60 minutes. Detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. The tissues were counterstained with hematoxylin and mounted with neutral balsam mounting medium.

  • CRBN Antibody (YA6516) Product Image
    MedchemExpress Validation

    Tyramide signaling amplification based immunofluorescence was performed on paraffin-embedded Mouse Brain tissue using CRBN antibody. Antigen retrieval was performed in EDTA buffer pH 9.0 (95 °C, 20 min) followed by cooling to RT. Then incubated with primary antibody (HY-P86823, 1:300 dilution) at room temperature for 60 minutes and HRP conjugated secondary antibody for 10 minutes. Fluorescence was then developed with Vari Fluor 532 TSA (200×)（HY-D1832）. The tissues were counterstained with DAPI and mounted with Anti-fade mounting medium.

  • CRBN Antibody (YA6516) Product Image
    MedchemExpress Validation

    Tyramide signaling amplification based immunofluorescence was performed on paraffin-embedded Mouse Brain tissue using CRBN antibody. Antigen retrieval was performed in EDTA buffer pH 9.0 (95 °C, 20 min) followed by cooling to RT. Then incubated with primary antibody (HY-P86823, 1:300 dilution) at room temperature for 60 minutes and HRP conjugated secondary antibody for 10 minutes. Fluorescence was then developed with Vari Fluor 532 TSA (200×)（HY-D1832）. The tissues were counterstained with DAPI and mounted with Anti-fade mounting medium.

  • CRBN Antibody (YA6516) Product Image WB
  • CRBN Antibody (YA6516) Product Image IHC-P
  • CRBN Antibody (YA6516) Product Image IHC-P
  • CRBN Antibody (YA6516) Product Image mIHC
  • CRBN Antibody (YA6516) Product Image mIHC