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  4. Prostate Specific Antigen Antibody (YA1537)

Prostate Specific Antigen Antibody (YA1537)

Cat. No.: HY-P81792
COA User Guide for Antibodies Technical Support

Prostate Specific Antigen Antibody (YA1537) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to Prostate Specific Antigen.

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Top Publications Citing Use of Products
  • WB: Western Blot;
  • IHC-P: Immunohistochemistry-Paraffin;
  • IHC-F: Immunohistochemistry-Frozen;
  • ICC/IF: Immunocytochemistry/Immunofluorescence;
  • IF-Tissue: Immunofluorescence-Tissue;
  • mIHC: Multiplex Immunohistochemical;
  • IP: Immunoprecipitation;
  • ChIP: Chromatin Immunoprecipitation;
  • FC: Flow Cytometry;
  • ELISA: Enzyme Linked Immunosorbent Assay
  • Product Detail

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  • Description

Description

Prostate Specific Antigen Antibody (YA1537) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to Prostate Specific Antigen.

Host

Rabbit

Clonality

Recombinant,Monoclonal

Masse moléculaire
Predicted band size: 29 kDa;
Observed band size: 34 kDa
Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.
Species Reactivity
Human
SwissProt ID
Gene ID
Immunogen

A synthesized peptide derived from human PSA/KLK3 aa215-261/261.

Application &
Dilution Ratio
Application Dilution Ratio
WB
WB: Western Blot
1:500-1:1000
IHC-P
IHC-P: Immunohistochemistry-Paraffin
1:50-1:100
Sensitivity Endogenous Pureté Affinity Chromatography
Conjugation Non-conjugated Modification Unmodified
Isotype IgG  
Appearance

Liquid

Formulation

Supplied in 50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40%Glycerol, 0.01% sodium azide and 0.05% BSA.

Storage & Stability

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

Livraison

Shipping with blue ice.

Verification Image
ALL IHC-P mIHC
  • Immunohistochemical analysis of paraffin-embedded human prostate tissue using Prostate Specific Antigen Antibody (YA1537). The section was pre-treated using heat mediated antigen retrieval with Tris/EDTA buffer (pH 9.0) for 20 minutes. The tissues were probed with the primary antibody (HY-P81792, 1/100) overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with neutral balsam.

  • Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue using Prostate Specific Antigen Antibody (YA1537). The section was pre-treated using heat mediated antigen retrieval with Tris/EDTA buffer (pH 9.0) for 20 minutes. The tissues were probed with the primary antibody (HY-P81792, 1/100) overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with neutral balsam.

  • Tyramide signaling amplification based immunofluorescence analysis of paraffin-embedded human prostate tissue using Prostate Specific Antigen Antibody (YA1537) . The section was pre-treated using heat mediated antigen retrieval with Tris/EDTA buffer (pH 9.0) for 20 minutes. The tissues were probed with the primary antibody (HY-P81792, 1/400) overnight at 4℃. The detection was performed using an HRP conjugated compact polymer and fluorescent tyramide signal amplification system.Immunostaining was performed with Vari Fluor 594 TSA (200×)(HY-D1835). Tissues were counterstained with DAPI (blue) and mounted with Anti-fade fluorescence mounting medium.

  • Tyramide signaling amplification based immunofluorescence analysis of paraffin-embedded human prostate cancer tissue using Prostate Specific Antigen Antibody (YA1537) . The section was pre-treated using heat mediated antigen retrieval with Tris/EDTA buffer (pH 9.0) for 20 minutes. The tissues were probed with the primary antibody (HY-P81792, 1/400) overnight at 4℃. The detection was performed using an HRP conjugated compact polymer and fluorescent tyramide signal amplification system.Immunostaining was performed with Vari Fluor 594 TSA (200×)(HY-D1835). Tissues were counterstained with DAPI (blue) and mounted with Anti-fade fluorescence mounting medium.

Background
Function:Hydrolyzes semenogelin-1 thus leading to the liquefaction of the seminal coagulum
Subcellular Localization:Secreted
Isoforms & Post-Translational Modification:P07288 has 5 isomers: P07288-1: 28741 Da (predicted); P07288-2: 26317 Da (predicted); P07288-3: 23725 Da (predicted); P07288-4: 7616 Da (predicted); P07288-5: 25116 Da (predicted).
Subunit:Forms a heterodimer with SERPINA5
RRID
Database

Entrez Gene: 354 Human

SwissProt: P07288 Human

OMIM: 176820 Human

Research Field

Neuroscience

Synonyms
KLK3; APS; Prostate-specific antigen; PSA; Gamma-seminoprotein; Seminin; Kallikrein-3; P-30 antigen; Semenogelase
Documentation

Prostate Specific Antigen Antibody (YA1537) Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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Nom du produit:
Prostate Specific Antigen Antibody (YA1537)
Cat. No.:
HY-P81792
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