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Prostate Specific Antigen Antibody (YA1537)

(Synonyms: KLK3; APS; Prostate-specific antigen; PSA; Gamma-seminoprotein; Seminin; Kallikrein-3; P-30 antigen; Semenogelase)
Customer Review

Based on 1 Customer Validation

Prostate Specific Antigen Antibody (YA1537) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to Prostate Specific Antigen.

For research use only. We do not sell to patients.
  • Host:

    Rabbit

  • Isotype:

    IgG

  • Application:

    WB, IHC-P

  • Reactivity :

    Human

  • Formulation:

    Supplied in 50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40%Glycerol, 0.01% sodium azide and 0.05% BSA.

  • Conjugation:
    Non-conjugated
ALL IHC-P mIHC
  • Experimental Validation Results for Prostate Specific Antigen Antibody (YA1537)
  • Experimental Validation Results for Prostate Specific Antigen Antibody (YA1537)
  • Experimental Validation Results for Prostate Specific Antigen Antibody (YA1537)
  • Experimental Validation Results for Prostate Specific Antigen Antibody (YA1537)
  • Prostate Specific Antigen Antibody (YA1537) Product Image
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To place orders, for customer services and technical support, please contact: MedChemExpress USA

Tel: 609-228-6898 E-mail: [email protected] [email protected]

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Related Products:
Top Publications Citing

Use of MCE Products

Applications

Application
WB Info
WB: Western Blot
IHC-P Info
IHC-P: Immunohistochemistry-Paraffin
Dilution Ratio 1:500-1:1000 1:50-1:100

Product Details

Description

Prostate Specific Antigen Antibody (YA1537) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to Prostate Specific Antigen.

  • Host Rabbit
  • Clonality Recombinant,Monoclonal
  • Species Reactivity
    Human
  • Observed Molecular Weight
    Observed band size: 34 kDa Info
    Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.
  • Calculated Molecular Weight Predicted band size: 29 kDa
Species Reactivity Database

Entrez Gene: 354 Human

SwissProt: P07288 Human

Immunogen

A synthesized peptide derived from human PSA/KLK3 aa215-261/261.

Sensitivity

Endogenous

Purification

Affinity Chromatography

Conjugation

Non-conjugated

Modification

Unmodified

Isotype

IgG

RRID

AB_3103895

Product Properties

  • Appearance

    Solution

  • Formulation

    Supplied in 50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40%Glycerol, 0.01% sodium azide and 0.05% BSA.

  • Concentration

    Batch-dependent, Please check the COA for the concentration of each lot. Check Lot Concentration

  • Storage & Stability

    Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

  • Shipping

    Shipping with blue ice.

Verification Images

  • Experimental Validation Results for Prostate Specific Antigen Antibody (YA1537)
    Immunohistochemical analysis of paraffin-embedded human prostate tissue using Prostate Specific Antigen Antibody (YA1537). The section was pre-treated using heat mediated antigen retrieval with Tris/EDTA buffer (pH 9.0) for 20 minutes. The tissues were probed with the primary antibody (HY-P81792, 1/100) overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with neutral balsam.
  • Experimental Validation Results for Prostate Specific Antigen Antibody (YA1537)
    Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue using Prostate Specific Antigen Antibody (YA1537). The section was pre-treated using heat mediated antigen retrieval with Tris/EDTA buffer (pH 9.0) for 20 minutes. The tissues were probed with the primary antibody (HY-P81792, 1/100) overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with neutral balsam.
  • Experimental Validation Results for Prostate Specific Antigen Antibody (YA1537)
    Tyramide signaling amplification based immunofluorescence analysis of paraffin-embedded human prostate tissue using Prostate Specific Antigen Antibody (YA1537) . The section was pre-treated using heat mediated antigen retrieval with Tris/EDTA buffer (pH 9.0) for 20 minutes. The tissues were probed with the primary antibody (HY-P81792, 1/400) overnight at 4℃. The detection was performed using an HRP conjugated compact polymer and fluorescent tyramide signal amplification system.Immunostaining was performed with Vari Fluor 594 TSA (200×)（HY-D1835）. Tissues were counterstained with DAPI (blue) and mounted with Anti-fade fluorescence mounting medium.
  • Experimental Validation Results for Prostate Specific Antigen Antibody (YA1537)
    Tyramide signaling amplification based immunofluorescence analysis of paraffin-embedded human prostate cancer tissue using Prostate Specific Antigen Antibody (YA1537) . The section was pre-treated using heat mediated antigen retrieval with Tris/EDTA buffer (pH 9.0) for 20 minutes. The tissues were probed with the primary antibody (HY-P81792, 1/400) overnight at 4℃. The detection was performed using an HRP conjugated compact polymer and fluorescent tyramide signal amplification system.Immunostaining was performed with Vari Fluor 594 TSA (200×)（HY-D1835）. Tissues were counterstained with DAPI (blue) and mounted with Anti-fade fluorescence mounting medium.

Background

  • Function

    Prostate Specific Antigen hydrolyzes semenogelin-1 thus leading to the liquefaction of the seminal coagulum

  • Subcellular Localization

    Secreted

  • Isoforms & Post-Translational Modification

    P07288 has 5 isomers: P07288-1: 28741 Da (predicted); P07288-2: 26317 Da (predicted); P07288-3: 23725 Da (predicted); P07288-4: 7616 Da (predicted); P07288-5: 25116 Da (predicted).

  • Subunit

    Forms a heterodimer with SERPINA5

  • SwissProt ID

    P07288

  • Gene ID
    354 [NCBI]
  • Synonyms

    KLK3; APS; Prostate-specific antigen; PSA; Gamma-seminoprotein; Seminin; Kallikrein-3; P-30 antigen; Semenogelase

  • Research Field

    Neuroscience

Prostate Specific Antigen Antibody (YA1537) Related Classifications

MOQ
Minimum order quantity
100 mg

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Measured by its ability to inhibit anti-CD3-induced proliferation of stimulated CTLL-2 mouse cytotoxic T cells. The ED50 this effect is 0.8023 μg/ml, corresponding to a specific activity is 1246.42 units/mg.

Assay Types
  • 1/16

    MedchemExpress Validation
     Immunohistochemical analysis of paraffin-embedded human prostate tissue using Prostate Specific Antigen Antibody (YA1537). The section was pre-treated using heat mediated antigen retrieval with Tris/EDTA buffer (pH 9.0) for 20 minutes. The tissues were probed with the primary antibody (HY-P81792, 1/100) overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with neutral balsam.

  • 1/16

    MedchemExpress Validation
     Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue using Prostate Specific Antigen Antibody (YA1537). The section was pre-treated using heat mediated antigen retrieval with Tris/EDTA buffer (pH 9.0) for 20 minutes. The tissues were probed with the primary antibody (HY-P81792, 1/100) overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with neutral balsam.

  • 1/16

    MedchemExpress Validation
     Tyramide signaling amplification based immunofluorescence analysis of paraffin-embedded human prostate tissue using Prostate Specific Antigen Antibody (YA1537) . The section was pre-treated using heat mediated antigen retrieval with Tris/EDTA buffer (pH 9.0) for 20 minutes. The tissues were probed with the primary antibody (HY-P81792, 1/400) overnight at 4℃. The detection was performed using an HRP conjugated compact polymer and fluorescent tyramide signal amplification system.Immunostaining was performed with Vari Fluor 594 TSA (200×)（HY-D1835）. Tissues were counterstained with DAPI (blue) and mounted with Anti-fade fluorescence mounting medium.

  • 1/16

    MedchemExpress Validation
     Tyramide signaling amplification based immunofluorescence analysis of paraffin-embedded human prostate cancer tissue using Prostate Specific Antigen Antibody (YA1537) . The section was pre-treated using heat mediated antigen retrieval with Tris/EDTA buffer (pH 9.0) for 20 minutes. The tissues were probed with the primary antibody (HY-P81792, 1/400) overnight at 4℃. The detection was performed using an HRP conjugated compact polymer and fluorescent tyramide signal amplification system.Immunostaining was performed with Vari Fluor 594 TSA (200×)（HY-D1835）. Tissues were counterstained with DAPI (blue) and mounted with Anti-fade fluorescence mounting medium.

  • Prostate Specific Antigen Antibody (YA1537) Product Image
    1/16
  • Experimental Validation Results for Prostate Specific Antigen Antibody (YA1537)
  • Experimental Validation Results for Prostate Specific Antigen Antibody (YA1537)
  • Experimental Validation Results for Prostate Specific Antigen Antibody (YA1537)
  • Experimental Validation Results for Prostate Specific Antigen Antibody (YA1537)
  • Prostate Specific Antigen Antibody (YA1537) Product Image
  • Experimental Validation Results for Prostate Specific Antigen Antibody (YA1537)
    1/1
    MedchemExpress Validation
     Immunohistochemical analysis of paraffin-embedded human prostate tissue using Prostate Specific Antigen Antibody (YA1537). The section was pre-treated using heat mediated antigen retrieval with Tris/EDTA buffer (pH 9.0) for 20 minutes. The tissues were probed with the primary antibody (HY-P81792, 1/100) overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with neutral balsam.
  • Experimental Validation Results for Prostate Specific Antigen Antibody (YA1537)
    1/1
    MedchemExpress Validation
     Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue using Prostate Specific Antigen Antibody (YA1537). The section was pre-treated using heat mediated antigen retrieval with Tris/EDTA buffer (pH 9.0) for 20 minutes. The tissues were probed with the primary antibody (HY-P81792, 1/100) overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with neutral balsam.
  • Experimental Validation Results for Prostate Specific Antigen Antibody (YA1537)
    1/1
    MedchemExpress Validation
     Tyramide signaling amplification based immunofluorescence analysis of paraffin-embedded human prostate tissue using Prostate Specific Antigen Antibody (YA1537) . The section was pre-treated using heat mediated antigen retrieval with Tris/EDTA buffer (pH 9.0) for 20 minutes. The tissues were probed with the primary antibody (HY-P81792, 1/400) overnight at 4℃. The detection was performed using an HRP conjugated compact polymer and fluorescent tyramide signal amplification system.Immunostaining was performed with Vari Fluor 594 TSA (200×)（HY-D1835）. Tissues were counterstained with DAPI (blue) and mounted with Anti-fade fluorescence mounting medium.
  • Experimental Validation Results for Prostate Specific Antigen Antibody (YA1537)
    1/1
    MedchemExpress Validation
     Tyramide signaling amplification based immunofluorescence analysis of paraffin-embedded human prostate cancer tissue using Prostate Specific Antigen Antibody (YA1537) . The section was pre-treated using heat mediated antigen retrieval with Tris/EDTA buffer (pH 9.0) for 20 minutes. The tissues were probed with the primary antibody (HY-P81792, 1/400) overnight at 4℃. The detection was performed using an HRP conjugated compact polymer and fluorescent tyramide signal amplification system.Immunostaining was performed with Vari Fluor 594 TSA (200×)（HY-D1835）. Tissues were counterstained with DAPI (blue) and mounted with Anti-fade fluorescence mounting medium.
  • Experimental Validation Results for Prostate Specific Antigen Antibody (YA1537)
  • Experimental Validation Results for Prostate Specific Antigen Antibody (YA1537)
  • Experimental Validation Results for Prostate Specific Antigen Antibody (YA1537)
  • Experimental Validation Results for Prostate Specific Antigen Antibody (YA1537)
  • Prostate Specific Antigen Antibody (YA1537) Product Image
    MedchemExpress Validation

    Immunohistochemical analysis of paraffin-embedded human prostate tissue using Prostate Specific Antigen Antibody (YA1537). The section was pre-treated using heat mediated antigen retrieval with Tris/EDTA buffer (pH 9.0) for 20 minutes. The tissues were probed with the primary antibody (HY-P81792, 1/100) overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with neutral balsam.

  • Prostate Specific Antigen Antibody (YA1537) Product Image
    MedchemExpress Validation

    Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue using Prostate Specific Antigen Antibody (YA1537). The section was pre-treated using heat mediated antigen retrieval with Tris/EDTA buffer (pH 9.0) for 20 minutes. The tissues were probed with the primary antibody (HY-P81792, 1/100) overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with neutral balsam.

  • Prostate Specific Antigen Antibody (YA1537) Product Image
    MedchemExpress Validation

    Tyramide signaling amplification based immunofluorescence analysis of paraffin-embedded human prostate tissue using Prostate Specific Antigen Antibody (YA1537) . The section was pre-treated using heat mediated antigen retrieval with Tris/EDTA buffer (pH 9.0) for 20 minutes. The tissues were probed with the primary antibody (HY-P81792, 1/400) overnight at 4℃. The detection was performed using an HRP conjugated compact polymer and fluorescent tyramide signal amplification system.Immunostaining was performed with Vari Fluor 594 TSA (200×)（HY-D1835）. Tissues were counterstained with DAPI (blue) and mounted with Anti-fade fluorescence mounting medium.

  • Prostate Specific Antigen Antibody (YA1537) Product Image
    MedchemExpress Validation

    Tyramide signaling amplification based immunofluorescence analysis of paraffin-embedded human prostate cancer tissue using Prostate Specific Antigen Antibody (YA1537) . The section was pre-treated using heat mediated antigen retrieval with Tris/EDTA buffer (pH 9.0) for 20 minutes. The tissues were probed with the primary antibody (HY-P81792, 1/400) overnight at 4℃. The detection was performed using an HRP conjugated compact polymer and fluorescent tyramide signal amplification system.Immunostaining was performed with Vari Fluor 594 TSA (200×)（HY-D1835）. Tissues were counterstained with DAPI (blue) and mounted with Anti-fade fluorescence mounting medium.

  • Prostate Specific Antigen Antibody (YA1537) Product Image IHC-P
  • Prostate Specific Antigen Antibody (YA1537) Product Image IHC-P
  • Prostate Specific Antigen Antibody (YA1537) Product Image mIHC
  • Prostate Specific Antigen Antibody (YA1537) Product Image mIHC