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HBP1 Antibody

(Synonyms: HMG box-containing protein 1, HMG box transcription factor 1, High mobility group box transcription factor 1, HBP1)

HBP1 Antibody is a Rabbit-derived and non-conjugated IgG Polyclonal antibody, targeting to HBP1.

For research use only. We do not sell to patients.
  • Host:

    Rabbit

  • Isotype:

    IgG

  • Application:

    WB, IHC-P

  • Reactivity :

    Human, Mouse, Rat

  • Formulation:

    Supplied in PBS (pH 7.4), containing 30% glycerol, and 0.01% sodium azide.

  • Conjugation:
    Non-conjugated
  • Experimental Validation Results for HBP1 Antibody
  • HBP1 Antibody Product Image
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To place orders, for customer services and technical support, please contact: MedChemExpress USA

Tel: 609-228-6898 E-mail: [email protected] [email protected]

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Use of MCE Products

Applications

Application
WB Info
WB: Western Blot
IHC-P Info
IHC-P: Immunohistochemistry-Paraffin
Dilution Ratio 1:1000-2000 1:100-200

Product Details

Description

HBP1 Antibody is a Rabbit-derived and non-conjugated IgG Polyclonal antibody, targeting to HBP1.

  • Host Rabbit
  • Clonality Polyclonal
  • Species Reactivity
    Human, Mouse, Rat
  • Observed Molecular Weight
    Observed band size: 57; 80 kDa Info
    Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.
  • Calculated Molecular Weight Predicted band size: 57 kDa
Immunogen

Synthetic peptide corresponding to the C-term region of human HBP1.

Sensitivity

Endogenous

Purification

affinity purified.

Conjugation

Non-conjugated

Modification

Unmodified

Isotype

IgG

Product Properties

  • Appearance

    Solution

  • Formulation

    Supplied in PBS (pH 7.4), containing 30% glycerol, and 0.01% sodium azide.

  • Storage & Stability

    Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

  • Shipping

    Shipping with blue ice.

Verification Images

  • Experimental Validation Results for HBP1 Antibody
    Western blot analysis of extracts from Jurkat (lane 2, 40ug), U251 (lane3, 40μg) using HBP1 Antibody (HY-P811032). Proteins were transferred to a PVDF membrane and blocked with 5% BSA in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P80993, 1/10,000) was used in 5% BSA in TBST at 4°C overnight. Goat Anti-Rabbit IgG-HRP Secondary Antibody (HY-P8001,1/10,000) was used for 1 hour at room temperature.

Background

  • Function

    HBP1 is a Transcriptional repressor that binds to the promoter region of target genes. Plays a role in the regulation of the cell cycle and of the Wnt pathway. Binds preferentially to the sequence 5'-TTCATTCATTCA-3'. Binding to the histone H1.0 promoter is enhanced by interaction with RB1. Disrupts the interaction between DNA and TCF4

  • Subcellular Localization

    Nucleus

  • Isoforms & Post-Translational Modification

    HBP1 has 3 isoforms, O60381-1: amino acid length is 514, molecular weight is 57645 Da (predicted); O60381-2: amino acid length is 462, molecular weight is 51433 Da (predicted); O60381-3: amino acid length is 514, molecular weight is 57699 Da (predicted).
    Ubiquitinated by the CTLH E3 ubiquitin-protein ligase complex, leading to subsequent proteasomal degradation.

  • Subunit

    Binds the second PAH repeat of SIN3A (Probable).

  • SwissProt ID

    O60381

  • Gene ID
    26959 [NCBI]
  • Synonyms

    HMG box-containing protein 1, HMG box transcription factor 1, High mobility group box transcription factor 1, HBP1

HBP1 Antibody Related Classifications

MOQ
Minimum order quantity
100 mg

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Measured by its ability to inhibit anti-CD3-induced proliferation of stimulated CTLL-2 mouse cytotoxic T cells. The ED50 this effect is 0.8023 μg/ml, corresponding to a specific activity is 1246.42 units/mg.

Assay Types
  • 1/16

    MedChemExpress Validation
     Western blot analysis of extracts from Jurkat (lane 2, 40ug), U251 (lane3, 40μg) using HBP1 Antibody (HY-P811032). Proteins were transferred to a PVDF membrane and blocked with 5% BSA in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P80993, 1/10,000) was used in 5% BSA in TBST at 4°C overnight. Goat Anti-Rabbit IgG-HRP Secondary Antibody (HY-P8001,1/10,000) was used for 1 hour at room temperature.

  • HBP1 Antibody Product Image
    1/16
  • Experimental Validation Results for HBP1 Antibody
  • HBP1 Antibody Product Image
  • Experimental Validation Results for HBP1 Antibody
    1/1
    MedChemExpress Validation
     Western blot analysis of extracts from Jurkat (lane 2, 40ug), U251 (lane3, 40μg) using HBP1 Antibody (HY-P811032). Proteins were transferred to a PVDF membrane and blocked with 5% BSA in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P80993, 1/10,000) was used in 5% BSA in TBST at 4°C overnight. Goat Anti-Rabbit IgG-HRP Secondary Antibody (HY-P8001,1/10,000) was used for 1 hour at room temperature.
  • Experimental Validation Results for HBP1 Antibody
  • HBP1 Antibody Product Image
    MedChemExpress Validation

    Western blot analysis of extracts from Jurkat (lane 2, 40ug), U251 (lane3, 40μg) using HBP1 Antibody (HY-P811032). Proteins were transferred to a PVDF membrane and blocked with 5% BSA in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P80993, 1/10,000) was used in 5% BSA in TBST at 4°C overnight. Goat Anti-Rabbit IgG-HRP Secondary Antibody (HY-P8001,1/10,000) was used for 1 hour at room temperature.

  • HBP1 Antibody Product Image WB