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Yes1 Antibody (YA1897)

(Synonyms: YES1; YES; Tyrosine-protein kinase Yes; Proto-oncogene c-Yes; p61-Yes)
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Based on 1 publication(s) in Google Scholar

Yes1 Antibody (YA1897) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to Yes1.

For research use only. We do not sell to patients.
  • Host:

    Rabbit

  • Isotype:

    IgG

  • Application:

    WB, IHC-P, ICC/IF, IP

  • Reactivity :

    Human, Mouse, Rat

  • Formulation:

    Supplied in PBS(pH 7.4), 150mM sodium chloride, 0.05% BSA, 0.02% sodium azide and 50% glycerol.

  • Conjugation:
    Non-conjugated
ALL IHC-P mIHC
  • Experimental Validation Results for Yes1 Antibody (YA1897)
  • Experimental Validation Results for Yes1 Antibody (YA1897)
  • Experimental Validation Results for Yes1 Antibody (YA1897)
  • Experimental Validation Results for Yes1 Antibody (YA1897)
  • Experimental Validation Results for Yes1 Antibody (YA1897)
  • Experimental Validation Results for Yes1 Antibody (YA1897)
  • Experimental Validation Results for Yes1 Antibody (YA1897)
  • Yes1 Antibody (YA1897) Product Image
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Applications

Application
WB Info
WB: Western Blot
IHC-P Info
IHC-P: Immunohistochemistry-Paraffin
ICC/IF Info
ICC/IF: Immunocytochemistry/
Immunofluorescence
IP Info
IP: Immunoprecipitation
Dilution Ratio 1:1000-2000 1:100-200 1:50-1:200 1:50

Product Details

Description

Yes1 Antibody (YA1897) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to Yes1.

  • Host Rabbit
  • Clonality Recombinant,Monoclonal
  • Species Reactivity
    Human, Mouse, Rat
  • Observed Molecular Weight
    Observed band size: 61 kDa Info
    Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.
  • Calculated Molecular Weight Predicted band size: 61 kDa
Species Reactivity Database
Immunogen

A synthesized peptide derived from human Yes1 aa500-543/543.

Sensitivity

Endogenous

Purification

Affinity Chromatography

Conjugation

Non-conjugated

Modification

Unmodified

Isotype

IgG

RRID

AB_3104265

Product Properties

  • Appearance

    Solution

  • Formulation

    Supplied in PBS(pH 7.4), 150mM sodium chloride, 0.05% BSA, 0.02% sodium azide and 50% glycerol.

  • Concentration

    Batch-dependent, Please check the COA for the concentration of each lot. Check Lot Concentration

  • Storage & Stability

    Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

  • Shipping

    Shipping with blue ice.

Verification Images

  • Experimental Validation Results for Yes1 Antibody (YA1897)
    Immunohistochemical analysis of paraffin-embedded human breast tissue using Yes1 Antibody (YA1897). The section was pre-treated using heat mediated antigen retrieval with Tris/EDTA buffer (pH 9.0) for 20 minutes. The tissues were probed with the primary antibody (HY-P82152, 1/100) overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with neutral balsam.
  • Experimental Validation Results for Yes1 Antibody (YA1897)
    Immunohistochemical analysis of paraffin-embedded human placenta tissue using Yes1 Antibody (YA1897). The section was pre-treated using heat mediated antigen retrieval with Tris/EDTA buffer (pH 9.0) for 20 minutes. The tissues were probed with the primary antibody (HY-P82152, 1/100) overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with neutral balsam.
  • Experimental Validation Results for Yes1 Antibody (YA1897)
    Immunohistochemical analysis of paraffin-embedded human kidney tissue using Yes1 Antibody (YA1897). The section was pre-treated using heat mediated antigen retrieval with Tris/EDTA buffer (pH 9.0) for 20 minutes. The tissues were probed with the primary antibody (HY-P82152, 1/100) overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with neutral balsam.
  • Experimental Validation Results for Yes1 Antibody (YA1897)
    Tyramide signaling amplification based immunofluorescence analysis of paraffin-embedded human breast tissue using Yes1 Antibody (YA1897). The section was pre-treated using heat mediated antigen retrieval with Tris/EDTA buffer (pH 9.0) for 20 minutes. The tissues were probed with the primary antibody (HY-P82152, 1/400) overnight at 4℃. The detection was performed using an HRP conjugated compact polymer and fluorescent tyramide signal amplification system.Immunostaining was performed with Vari Fluor 594 TSA (200×)（HY-D1835）. Tissues were counterstained with DAPI (blue) and mounted with Anti-fade fluorescence mounting medium.
  • Experimental Validation Results for Yes1 Antibody (YA1897)
    Tyramide signaling amplification based immunofluorescence analysis of paraffin-embedded human placenta tissue using Yes1 Antibody (YA1897). The section was pre-treated using heat mediated antigen retrieval with Tris/EDTA buffer (pH 9.0) for 20 minutes. The tissues were probed with the primary antibody (HY-P82152, 1/400) overnight at 4℃. The detection was performed using an HRP conjugated compact polymer and fluorescent tyramide signal amplification system.Immunostaining was performed with Vari Fluor 594 TSA (200×)（HY-D1835）. Tissues were counterstained with DAPI (blue) and mounted with Anti-fade fluorescence mounting medium.
  • Experimental Validation Results for Yes1 Antibody (YA1897)
    Tyramide signaling amplification based immunofluorescence analysis of paraffin-embedded human kidney tissue using Yes1 Antibody (YA1897). The section was pre-treated using heat mediated antigen retrieval with Tris/EDTA buffer (pH 9.0) for 20 minutes. The tissues were probed with the primary antibody (HY-P82152, 1/400) overnight at 4℃. The detection was performed using an HRP conjugated compact polymer and fluorescent tyramide signal amplification system.Immunostaining was performed with Vari Fluor 594 TSA (200×)（HY-D1835）. Tissues were counterstained with DAPI (blue) and mounted with Anti-fade fluorescence mounting medium.
  • Experimental Validation Results for Yes1 Antibody (YA1897)
    Tyramide signaling amplification based immunofluorescence analysis of paraffin-embedded human prostate tissue using Yes1 Antibody (YA1897). The section was pre-treated using heat mediated antigen retrieval with Tris/EDTA buffer (pH 9.0) for 20 minutes. The tissues were probed with the primary antibody (HY-P82152, 1/300) overnight at 4℃. The detection was performed using an HRP conjugated compact polymer and fluorescent tyramide signal amplification system.Immunostaining was performed using a Bicolor signal amplification fluorescence staining kit. Tissues were counterstained with DAPI (blue) and mounted with Anti-fade fluorescence mounting medium.

Background

  • Function

    Yes1 is a Non-receptor protein tyrosine kinase that is involved in the regulation of cell growth and survival, apoptosis, cell-cell adhesion, cytoskeleton remodeling, and differentiation. Stimulation by receptor tyrosine kinases (RTKs) including EGFR, PDGFR, CSF1R and FGFR leads to recruitment of YES1 to the phosphorylated receptor, and activation and phosphorylation of downstream substrates. Upon EGFR activation, promotes the phosphorylation of PARD3 to favor epithelial tight junction assembly. Participates in the phosphorylation of specific junctional components such as CTNND1 by stimulating the FYN and FER tyrosine kinases at cell-cell contacts. Upon T-cell stimulation by CXCL12, phosphorylates collapsin response mediator protein 2/DPYSL2 and induces T-cell migration. Participates in CD95L/FASLG signaling pathway and mediates AKT-mediated cell migration. Plays a role in cell cycle progression by phosphorylating the cyclin-dependent kinase 4/CDK4 thus regulating the G1 phase. Also involved in G2/M progression and cytokinesis. Catalyzes phosphorylation of organic cation transporter OCT2 which induces its transport activity[1].

  • Subcellular Localization

    Cell membrane; Cytoplasm, cytoskeleton, microtubule organizing center, centrosome; Cytoplasm, cytosol; Cell junction

  • Expression


    Tissue_specificity:In fetal tissues, this gene is expressed in the epithelial cells of the proximal tubules of the kidneys and the stomach, as well as in hematopoietic cells of the bone marrow and spleen. In humans, this gene is expressed in the epithelial cells of the proximal tubules of the kidneys and is present in keratinocytes of the basal layer of the epidermis.

  • Subunit

    Interacts with YAP1 and CSF1R (By similarity). Interacts with CTNND1; this interaction allows YES1-mediated activation of FYN and FER and subsequent phosphorylation of CTNND1 (By similarity). Interacts with FASLG. Interacts with IL6ST/gp130 (PubMed:25731159). Interacts with SCRIB, when YES1 is in a closed conformation; the interaction facilitates YES1 autophosphorylation (PubMed:33730553)

  • SwissProt ID

    P07947

  • Gene ID
    7525 [NCBI]
  • Synonyms

    YES1; YES; Tyrosine-protein kinase Yes; Proto-oncogene c-Yes; p61-Yes

  • Research Field

    Signal Transduction

References

[1]. Sprowl JA, et al. A phosphotyrosine switch regulates organic cation transporters. Nat Commun. 2016 Mar 16;7:10880.

Yes1 Antibody (YA1897) Related Classifications

MOQ
Minimum order quantity
100 mg

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Measured by its ability to inhibit anti-CD3-induced proliferation of stimulated CTLL-2 mouse cytotoxic T cells. The ED50 this effect is 0.8023 μg/ml, corresponding to a specific activity is 1246.42 units/mg.

Assay Types
  • 1/16

    MedchemExpress Validation
     Immunohistochemical analysis of paraffin-embedded human breast tissue using Yes1 Antibody (YA1897). The section was pre-treated using heat mediated antigen retrieval with Tris/EDTA buffer (pH 9.0) for 20 minutes. The tissues were probed with the primary antibody (HY-P82152, 1/100) overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with neutral balsam.

  • 1/16

    MedchemExpress Validation
     Immunohistochemical analysis of paraffin-embedded human placenta tissue using Yes1 Antibody (YA1897). The section was pre-treated using heat mediated antigen retrieval with Tris/EDTA buffer (pH 9.0) for 20 minutes. The tissues were probed with the primary antibody (HY-P82152, 1/100) overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with neutral balsam.

  • 1/16

    MedchemExpress Validation
     Immunohistochemical analysis of paraffin-embedded human kidney tissue using Yes1 Antibody (YA1897). The section was pre-treated using heat mediated antigen retrieval with Tris/EDTA buffer (pH 9.0) for 20 minutes. The tissues were probed with the primary antibody (HY-P82152, 1/100) overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with neutral balsam.

  • 1/16

    MedchemExpress Validation
     Tyramide signaling amplification based immunofluorescence analysis of paraffin-embedded human breast tissue using Yes1 Antibody (YA1897). The section was pre-treated using heat mediated antigen retrieval with Tris/EDTA buffer (pH 9.0) for 20 minutes. The tissues were probed with the primary antibody (HY-P82152, 1/400) overnight at 4℃. The detection was performed using an HRP conjugated compact polymer and fluorescent tyramide signal amplification system.Immunostaining was performed with Vari Fluor 594 TSA (200×)（HY-D1835）. Tissues were counterstained with DAPI (blue) and mounted with Anti-fade fluorescence mounting medium.

  • 1/16

    MedchemExpress Validation
     Tyramide signaling amplification based immunofluorescence analysis of paraffin-embedded human placenta tissue using Yes1 Antibody (YA1897). The section was pre-treated using heat mediated antigen retrieval with Tris/EDTA buffer (pH 9.0) for 20 minutes. The tissues were probed with the primary antibody (HY-P82152, 1/400) overnight at 4℃. The detection was performed using an HRP conjugated compact polymer and fluorescent tyramide signal amplification system.Immunostaining was performed with Vari Fluor 594 TSA (200×)（HY-D1835）. Tissues were counterstained with DAPI (blue) and mounted with Anti-fade fluorescence mounting medium.

  • 1/16

    MedchemExpress Validation
     Tyramide signaling amplification based immunofluorescence analysis of paraffin-embedded human kidney tissue using Yes1 Antibody (YA1897). The section was pre-treated using heat mediated antigen retrieval with Tris/EDTA buffer (pH 9.0) for 20 minutes. The tissues were probed with the primary antibody (HY-P82152, 1/400) overnight at 4℃. The detection was performed using an HRP conjugated compact polymer and fluorescent tyramide signal amplification system.Immunostaining was performed with Vari Fluor 594 TSA (200×)（HY-D1835）. Tissues were counterstained with DAPI (blue) and mounted with Anti-fade fluorescence mounting medium.

  • 1/16

    MedChemExpress Validation
     Tyramide signaling amplification based immunofluorescence analysis of paraffin-embedded human prostate tissue using Yes1 Antibody (YA1897). The section was pre-treated using heat mediated antigen retrieval with Tris/EDTA buffer (pH 9.0) for 20 minutes. The tissues were probed with the primary antibody (HY-P82152, 1/300) overnight at 4℃. The detection was performed using an HRP conjugated compact polymer and fluorescent tyramide signal amplification system.Immunostaining was performed using a Bicolor signal amplification fluorescence staining kit. Tissues were counterstained with DAPI (blue) and mounted with Anti-fade fluorescence mounting medium.

  • Yes1 Antibody (YA1897) Product Image
    1/16
  • Experimental Validation Results for Yes1 Antibody (YA1897)
  • Experimental Validation Results for Yes1 Antibody (YA1897)
  • Experimental Validation Results for Yes1 Antibody (YA1897)
  • Experimental Validation Results for Yes1 Antibody (YA1897)
  • Experimental Validation Results for Yes1 Antibody (YA1897)
  • Experimental Validation Results for Yes1 Antibody (YA1897)
  • Experimental Validation Results for Yes1 Antibody (YA1897)
  • Yes1 Antibody (YA1897) Product Image
  • Experimental Validation Results for Yes1 Antibody (YA1897)
    1/1
    MedchemExpress Validation
     Immunohistochemical analysis of paraffin-embedded human breast tissue using Yes1 Antibody (YA1897). The section was pre-treated using heat mediated antigen retrieval with Tris/EDTA buffer (pH 9.0) for 20 minutes. The tissues were probed with the primary antibody (HY-P82152, 1/100) overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with neutral balsam.
  • Experimental Validation Results for Yes1 Antibody (YA1897)
    1/1
    MedchemExpress Validation
     Immunohistochemical analysis of paraffin-embedded human placenta tissue using Yes1 Antibody (YA1897). The section was pre-treated using heat mediated antigen retrieval with Tris/EDTA buffer (pH 9.0) for 20 minutes. The tissues were probed with the primary antibody (HY-P82152, 1/100) overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with neutral balsam.
  • Experimental Validation Results for Yes1 Antibody (YA1897)
    1/1
    MedchemExpress Validation
     Immunohistochemical analysis of paraffin-embedded human kidney tissue using Yes1 Antibody (YA1897). The section was pre-treated using heat mediated antigen retrieval with Tris/EDTA buffer (pH 9.0) for 20 minutes. The tissues were probed with the primary antibody (HY-P82152, 1/100) overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with neutral balsam.
  • Experimental Validation Results for Yes1 Antibody (YA1897)
    1/1
    MedchemExpress Validation
     Tyramide signaling amplification based immunofluorescence analysis of paraffin-embedded human breast tissue using Yes1 Antibody (YA1897). The section was pre-treated using heat mediated antigen retrieval with Tris/EDTA buffer (pH 9.0) for 20 minutes. The tissues were probed with the primary antibody (HY-P82152, 1/400) overnight at 4℃. The detection was performed using an HRP conjugated compact polymer and fluorescent tyramide signal amplification system.Immunostaining was performed with Vari Fluor 594 TSA (200×)（HY-D1835）. Tissues were counterstained with DAPI (blue) and mounted with Anti-fade fluorescence mounting medium.
  • Experimental Validation Results for Yes1 Antibody (YA1897)
    1/1
    MedchemExpress Validation
     Tyramide signaling amplification based immunofluorescence analysis of paraffin-embedded human placenta tissue using Yes1 Antibody (YA1897). The section was pre-treated using heat mediated antigen retrieval with Tris/EDTA buffer (pH 9.0) for 20 minutes. The tissues were probed with the primary antibody (HY-P82152, 1/400) overnight at 4℃. The detection was performed using an HRP conjugated compact polymer and fluorescent tyramide signal amplification system.Immunostaining was performed with Vari Fluor 594 TSA (200×)（HY-D1835）. Tissues were counterstained with DAPI (blue) and mounted with Anti-fade fluorescence mounting medium.
  • Experimental Validation Results for Yes1 Antibody (YA1897)
    1/1
    MedchemExpress Validation
     Tyramide signaling amplification based immunofluorescence analysis of paraffin-embedded human kidney tissue using Yes1 Antibody (YA1897). The section was pre-treated using heat mediated antigen retrieval with Tris/EDTA buffer (pH 9.0) for 20 minutes. The tissues were probed with the primary antibody (HY-P82152, 1/400) overnight at 4℃. The detection was performed using an HRP conjugated compact polymer and fluorescent tyramide signal amplification system.Immunostaining was performed with Vari Fluor 594 TSA (200×)（HY-D1835）. Tissues were counterstained with DAPI (blue) and mounted with Anti-fade fluorescence mounting medium.
  • Experimental Validation Results for Yes1 Antibody (YA1897)
    1/1
    MedChemExpress Validation
     Tyramide signaling amplification based immunofluorescence analysis of paraffin-embedded human prostate tissue using Yes1 Antibody (YA1897). The section was pre-treated using heat mediated antigen retrieval with Tris/EDTA buffer (pH 9.0) for 20 minutes. The tissues were probed with the primary antibody (HY-P82152, 1/300) overnight at 4℃. The detection was performed using an HRP conjugated compact polymer and fluorescent tyramide signal amplification system.Immunostaining was performed using a Bicolor signal amplification fluorescence staining kit. Tissues were counterstained with DAPI (blue) and mounted with Anti-fade fluorescence mounting medium.
  • Experimental Validation Results for Yes1 Antibody (YA1897)
  • Experimental Validation Results for Yes1 Antibody (YA1897)
  • Experimental Validation Results for Yes1 Antibody (YA1897)
  • Experimental Validation Results for Yes1 Antibody (YA1897)
  • Experimental Validation Results for Yes1 Antibody (YA1897)
  • Experimental Validation Results for Yes1 Antibody (YA1897)
  • Experimental Validation Results for Yes1 Antibody (YA1897)
  • Yes1 Antibody (YA1897) Product Image
    MedchemExpress Validation

    Immunohistochemical analysis of paraffin-embedded human breast tissue using Yes1 Antibody (YA1897). The section was pre-treated using heat mediated antigen retrieval with Tris/EDTA buffer (pH 9.0) for 20 minutes. The tissues were probed with the primary antibody (HY-P82152, 1/100) overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with neutral balsam.

  • Yes1 Antibody (YA1897) Product Image
    MedchemExpress Validation

    Immunohistochemical analysis of paraffin-embedded human placenta tissue using Yes1 Antibody (YA1897). The section was pre-treated using heat mediated antigen retrieval with Tris/EDTA buffer (pH 9.0) for 20 minutes. The tissues were probed with the primary antibody (HY-P82152, 1/100) overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with neutral balsam.

  • Yes1 Antibody (YA1897) Product Image
    MedchemExpress Validation

    Immunohistochemical analysis of paraffin-embedded human kidney tissue using Yes1 Antibody (YA1897). The section was pre-treated using heat mediated antigen retrieval with Tris/EDTA buffer (pH 9.0) for 20 minutes. The tissues were probed with the primary antibody (HY-P82152, 1/100) overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with neutral balsam.

  • Yes1 Antibody (YA1897) Product Image
    MedchemExpress Validation

    Tyramide signaling amplification based immunofluorescence analysis of paraffin-embedded human breast tissue using Yes1 Antibody (YA1897). The section was pre-treated using heat mediated antigen retrieval with Tris/EDTA buffer (pH 9.0) for 20 minutes. The tissues were probed with the primary antibody (HY-P82152, 1/400) overnight at 4℃. The detection was performed using an HRP conjugated compact polymer and fluorescent tyramide signal amplification system.Immunostaining was performed with Vari Fluor 594 TSA (200×)（HY-D1835）. Tissues were counterstained with DAPI (blue) and mounted with Anti-fade fluorescence mounting medium.

  • Yes1 Antibody (YA1897) Product Image
    MedchemExpress Validation

    Tyramide signaling amplification based immunofluorescence analysis of paraffin-embedded human placenta tissue using Yes1 Antibody (YA1897). The section was pre-treated using heat mediated antigen retrieval with Tris/EDTA buffer (pH 9.0) for 20 minutes. The tissues were probed with the primary antibody (HY-P82152, 1/400) overnight at 4℃. The detection was performed using an HRP conjugated compact polymer and fluorescent tyramide signal amplification system.Immunostaining was performed with Vari Fluor 594 TSA (200×)（HY-D1835）. Tissues were counterstained with DAPI (blue) and mounted with Anti-fade fluorescence mounting medium.

  • Yes1 Antibody (YA1897) Product Image
    MedchemExpress Validation

    Tyramide signaling amplification based immunofluorescence analysis of paraffin-embedded human kidney tissue using Yes1 Antibody (YA1897). The section was pre-treated using heat mediated antigen retrieval with Tris/EDTA buffer (pH 9.0) for 20 minutes. The tissues were probed with the primary antibody (HY-P82152, 1/400) overnight at 4℃. The detection was performed using an HRP conjugated compact polymer and fluorescent tyramide signal amplification system.Immunostaining was performed with Vari Fluor 594 TSA (200×)（HY-D1835）. Tissues were counterstained with DAPI (blue) and mounted with Anti-fade fluorescence mounting medium.

  • Yes1 Antibody (YA1897) Product Image
    MedChemExpress Validation

    Tyramide signaling amplification based immunofluorescence analysis of paraffin-embedded human prostate tissue using Yes1 Antibody (YA1897). The section was pre-treated using heat mediated antigen retrieval with Tris/EDTA buffer (pH 9.0) for 20 minutes. The tissues were probed with the primary antibody (HY-P82152, 1/300) overnight at 4℃. The detection was performed using an HRP conjugated compact polymer and fluorescent tyramide signal amplification system.Immunostaining was performed using a Bicolor signal amplification fluorescence staining kit. Tissues were counterstained with DAPI (blue) and mounted with Anti-fade fluorescence mounting medium.

  • Yes1 Antibody (YA1897) Product Image IHC-P
  • Yes1 Antibody (YA1897) Product Image IHC-P
  • Yes1 Antibody (YA1897) Product Image IHC-P
  • Yes1 Antibody (YA1897) Product Image mIHC
  • Yes1 Antibody (YA1897) Product Image mIHC
  • Yes1 Antibody (YA1897) Product Image mIHC
  • Yes1 Antibody (YA1897) Product Image mIHC