BAG3 Antibody (YA2240)
(Synonyms: BAG3; BIS; BAG family molecular chaperone regulator 3; BAG-3; Bcl-2-associated athanogene 3; Bcl-2-binding protein Bis; Docking protein CAIR-1)BAG3 Antibody (YA2240) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to BAG3.
-
Host:
Rabbit
-
Isotype:
IgG
-
Application:
WB
-
Reactivity :
Human, Rat
-
Formulation:
Supplied in 50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40% Glycerol, 0.01% Sodium azide and 0.05% BSA
-
Conjugation:
Non-conjugated
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
Applications
| Application |
WB
WB: Western Blot
|
|---|---|
| Dilution Ratio | 1:500-1:1000 |
Product Details
BAG3 Antibody (YA2240) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to BAG3.
-
Host Rabbit
-
Clonality Recombinant, Monoclonal
-
Species ReactivityHuman, Rat
-
Observed Molecular WeightObserved band size: 80 kDa
Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.
-
Calculated Molecular Weight Predicted band size: 62 kDa
Recombinant protein of human Bag3
Endogenous
Affinity Purified
Non-conjugated
Unmodified
IgG
Product Properties
-
Appearance
Liquid
-
Formulation
Supplied in 50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40% Glycerol, 0.01% Sodium azide and 0.05% BSA
-
Storage & Stability
Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.
-
Shipping
Shipping with blue ice.
Background
-
Function
BAG3 is a Co-chaperone and adapter protein that connects different classes of molecular chaperones including heat shock proteins 70 (HSP70s), e.g. HSPA1A/HSP70 or HSPA8/HSC70, and small heat shock proteins (sHSPs), e.g. HSPB8. Acts as a nucleotide-exchange factor (NEF) promoting the release of ADP from HSP70s, thereby triggering client protein release. Nucleotide release is mediated via BAG3 binding to the nucleotide-binding domain (NBD) of HSP70s, whereas client release is mediated via binding to the substrate-binding domain (SBD). Has anti-apoptotic activity. Plays a role in the HSF1 nucleocytoplasmic transport[1][2][3][4][5][6].
-
Subcellular Localization
Nucleus; Cytoplasm
-
Subunit
Forms a ternary complex with HSPA1A/HSP70 and HSPB8, serving as scaffold subunit (PubMed:27884606). Component of the chaperone-assisted selective autophagy (CASA) complex consisting of BAG3, HSPA8/HSC70, HSPB8 and STUB1/CHIP (PubMed:20060297). Binds to the ATPase domain of HSP70 chaperones (PubMed:9873016). Interacts with BCL2 (PubMed:10597216). Interacts with phospholipase C-gamma proteins (PubMed:10980614). Interacts with DNAJB1 and DNAJB6 (PubMed:22366786, PubMed:30559338). Interacts (via BAG domain) with HSF1; this interaction occurs in normal and heat-shocked cells promoting HSF1 nucleocytoplasmic shuttling (PubMed:26159920). Interacts with HSPA8/HSC70 (via NBD) (PubMed:24318877, PubMed:27474739, PubMed:30559338). Interacts with HSPA1A (via NBD) and HSPA1B (via NBD) (PubMed:24318877, PubMed:30559338). Interacts (via WW domain 1) with SYNPO2 (via PPPY motif) (PubMed:23434281). Interacts with HSPB8 (PubMed:28144995, PubMed:30559338)
-
SwissProt ID
-
Synonyms
BAG3; BIS; BAG family molecular chaperone regulator 3; BAG-3; Bcl-2-associated athanogene 3; Bcl-2-binding protein Bis; Docking protein CAIR-1
-
Research Field
Cell Biology
Documentation
Powered by Bioz
