PIP4K2A Antibody (YA8512)(PBS only)

Cat. No.: HY-P88828A: Data Sheet User Guide for Antibodies
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PIP4K2A Antibody (YA8512) is a Mouse-derived and non-conjugated IgG1 monoclonal antibody, targeting to PIP4K2A.

For research use only. We do not sell to patients.

Size		Stock
50 μL		Get quote
100 μL		Get quote

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WB: Western Blot;
IHC-P: Immunohistochemistry-Paraffin;
IHC-F: Immunohistochemistry-Frozen;
ICC/IF: Immunocytochemistry/Immunofluorescence;
IF-Tissue: Immunofluorescence-Tissue;
mIHC: Multiplex Immunohistochemical;
IP: Immunoprecipitation;
ChIP: Chromatin Immunoprecipitation;
FC: Flow Cytometry;
ELISA: Enzyme Linked Immunosorbent Assay

Product Detail
Background
Documentation

Description

PIP4K2A Antibody (YA8512) is a Mouse-derived and non-conjugated IgG1 monoclonal antibody, targeting to PIP4K2A.

Host

Mouse

Molecular Weight

Predicted band size: 46 kDa

Species Reactivity

Human, Mouse, Rat

SwissProt ID

P48426

Immunogen

Full length human recombinant protein of human PIP4K2A produced in HEK293T cell.

Application &
Dilution Ratio

Application	Dilution Ratio
WB WB: Western Blot	1:500-2000
ICC/IF ICC/IF: Immunocytochemistry/Immunofluorescence	1:100-250
FC FC: Flow Cytometry	1:100

Purity	Affinity purified	Conjugation	Non-conjugated
Isotype	IgG1

Appearance

Liquid

Formulation

Supplied in PBS, pH 7.4.

Storage & Stability

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

Shipping

Shipping with blue ice.

Background

Function：Catalyzes the phosphorylation of phosphatidylinositol 5-phosphate (PtdIns5P) on the fourth hydroxyl of the myo-inositol ring, to form phosphatidylinositol 4,5-bisphosphate (PtdIns(4,5)P2) (PubMed:23326584, PubMed:9367159). Has both ATP- and GTP-dependent kinase activities (PubMed:26774281). May exert its function by regulating the levels of PtdIns5P, which functions in the cytosol by increasing AKT activity and in the nucleus signals through ING2 (PubMed:18364242). May regulate the pool of cytosolic PtdIns5P in response to the activation of tyrosine phosphorylation (By similarity). Required for lysosome-peroxisome membrane contacts and intracellular cholesterol transport through modulating peroxisomal PtdIns(4,5)P2 level (PubMed:29353240). In collaboration with PIP4K2B, has a role in mediating autophagy in times of nutrient stress (By similarity). Required for autophagosome-lysosome fusion and the regulation of cellular lipid metabolism (PubMed:31091439). May be involved in thrombopoiesis, and the terminal maturation of megakaryocytes and regulation of their size (By similarity). Negatively regulates insulin signaling through a catalytic-independent mechanism (PubMed:31091439). PIP4Ks interact with PIP5Ks and suppress PIP5K-mediated PtdIns(4,5)P2 synthesis and insulin-dependent conversion to PtdIns(3,4,5)P3 (PubMed:31091439)

Subcellular Localization：Cell membrane,Nucleus,Lysosome,Cytoplasm,Photoreceptor inner segment,Cell projection, cilium, photoreceptor outer segment

Expression：
Tissue_Specificity: Expressed ubiquitously, with high levels in the brain. Present in most tissues, except notably skeletal muscle and small intestine

Isoforms & Post-Translational Modification：P48426 has two isomers: P48426-1: 46225 Da (predicted); P48426-2: 39802 Da (predicted).
Phosphorylated in tyrosines

Subunit：Homodimer (PubMed:32130941)

Synonyms

PI5P4KA; PIP5K2A; PIP5KII-alpha; PIP5KIIA; PIPK

Documentation

User Guide for Antibodies (1077 KB)