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VGLUT1 Antibody (YA6880)

(Synonyms: BNPI antibody)
Customer Review

Based on 1 Customer Validation

VGLUT1 Antibody (YA6880) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to VGLUT1.

For research use only. We do not sell to patients.
  • Host:

    Rabbit

  • Isotype:

    IgG

  • Application:

    WB, IHC-F, IHC-P, IF-Tissue

  • Reactivity :

    Human, Mouse, Rat

  • Formulation:

    Supplied in PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

  • Conjugation:
    Non-conjugated
  • Experimental Validation Results for VGLUT1 Antibody (YA6880)
  • VGLUT1 Antibody (YA6880) Product Image
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To place orders, for customer services and technical support, please contact: MedChemExpress USA

Tel: 609-228-6898 E-mail: [email protected] [email protected]

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Use of MCE Products

Applications

Application
WB Info
WB: Western Blot
IHC-F Info
IHC-F: Immunohistochemistry-Frozen
IHC-P Info
IHC-P: Immunohistochemistry-Paraffin
IF-Tissue Info
IF-Tissue: Immunofluorescence-Tissue
Dilution Ratio 1:2000 1:500-1000 1:500-1:2000 1:500

Product Details

Description

VGLUT1 Antibody (YA6880) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to VGLUT1.

  • Host Rabbit
  • Clonality Recombinant,Monoclonal
  • Species Reactivity
    Human, Mouse, Rat Predicted Reactivity: Cynomolgus monkey,Pig
    Info
    Note: The predicted reactivity is for reference only and should not be considered a guarantee of product performance.
  • Observed Molecular Weight
    Observed band size: 62 kDa Info
    Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.
  • Calculated Molecular Weight Predicted band size: 62 kDa
Immunogen

Recombinant protein within Mouse VGLUT1 aa 1-560.

Purification

affinity purified.

Conjugation

Non-conjugated

Modification

Unmodified

Isotype

IgG

Product Properties

  • Appearance

    Liquid

  • Formulation

    Supplied in PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

  • Concentration

    Batch-dependent, Please check the COA for the concentration of each lot. Check Lot Concentration

  • Storage & Stability

    Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

  • Shipping

    Shipping with blue ice.

Verification Images

  • Experimental Validation Results for VGLUT1 Antibody (YA6880)
    Western blot analysis was performed on protein extracts (30 μg) from SH-SY5Y (lane 2), HeLa (lane 3), HepG2 (lane 4), and A549 (lane 5) using VGLUT1 antibody. Proteins were transferred onto a 0.45 μm PVDF membrane using the Trans-Blot® Turbo™ system for 13 min. The membrane was then blocked with 5% nonfat milk in TBST (HY-K1025) for 1 h at room temperature. Thhe primary antibody (1:2000) and loading control antibody GAPDH Antibody (HRP) (HY-P80954A) (1:5000) were diluted in 5% nonfat milk in TBST and incubated with the membrane overnight at 4°C. After washing, the membrane of primary antibody was incubated with HRP-conjugated goat anti-rabbit/mouse IgG secondary antibody (HY-P8001/HY-P8004) (1:5000) diluted in 5% nonfat milk in TBST for 1 h at room temperature. Protein bands were visualized using an Ultra High Sensitivity ECL detection kit (HY-K1005).

Background

  • Function

    VGLUT1 multifunctional transporter that transports L-glutamate as well as multiple ions such as chloride, proton, potassium, sodium and phosphate. At the synaptic vesicle membrane, mainly functions as an uniporter which transports preferentially L-glutamate but also phosphate from the cytoplasm into synaptic vesicles at presynaptic nerve terminals of excitatory neural cells. The L-glutamate or phosphate uniporter activity is electrogenic and is driven by the proton electrochemical gradient, mainly by the electrical gradient established by the vacuolar H(+)-ATPase across the synaptic vesicle membrane. In addition, functions as a chloride channel that allows a chloride permeation through the synaptic vesicle membrane that affects the proton electrochemical gradient and promotes synaptic vesicles acidification. Moreover, may function as a K(+)/H(+) antiport allowing to maintain the electrical gradient and to decrease chemical gradient and therefore sustain vesicular glutamate uptake. The vesicular K(+)/H(+) antiport activity is electroneutral. At the plasma membrane, following exocytosis, functions as a symporter of Na(+) and phosphate from the extracellular space to the cytoplasm allowing synaptic phosphate homeostasis regulation. The symporter activity is driven by an inside negative membrane potential and is electrogenic. Is necessary for synaptic signaling of visual-evoked responses from photoreceptors[1].

  • Subcellular Localization

    Cytoplasmic vesicle, secretory vesicle, synaptic vesicle membrane,Cell membrane,Synapse, synaptosome

  • Expression


    Tissue_Specificity: Expressed in several regions of the brain including amygdala, cerebellum, cerebral cortex, hippocampus, frontal lobe, medulla, occipital lobe, putamen and temporal lobe

  • Isoforms & Post-Translational Modification

    Q9P2U7 has three isomers: Q9P2U7-1: 61613 Da (predicted); Q9P2U7-2: 53946 Da (predicted); Q9P2U7-3: 59887 Da (predicted).

  • Subunit

    Interacts with SHANK3

  • SwissProt ID

    Q9P2U7

  • Synonyms

    BNPI antibody

VGLUT1 Antibody (YA6880) Related Classifications

MOQ
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100 mg

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Measured by its ability to inhibit anti-CD3-induced proliferation of stimulated CTLL-2 mouse cytotoxic T cells. The ED50 this effect is 0.8023 μg/ml, corresponding to a specific activity is 1246.42 units/mg.

Assay Types
  • 1/16

    MedChemExpress Validation
     Western blot analysis was performed on protein extracts (30 μg) from SH-SY5Y (lane 2), HeLa (lane 3), HepG2 (lane 4), and A549 (lane 5) using VGLUT1 antibody. Proteins were transferred onto a 0.45 μm PVDF membrane using the Trans-Blot® Turbo™ system for 13 min. The membrane was then blocked with 5% nonfat milk in TBST (HY-K1025) for 1 h at room temperature. Thhe primary antibody (1:2000) and loading control antibody GAPDH Antibody (HRP) (HY-P80954A) (1:5000) were diluted in 5% nonfat milk in TBST and incubated with the membrane overnight at 4°C. After washing, the membrane of primary antibody was incubated with HRP-conjugated goat anti-rabbit/mouse IgG secondary antibody (HY-P8001/HY-P8004) (1:5000) diluted in 5% nonfat milk in TBST for 1 h at room temperature. Protein bands were visualized using an Ultra High Sensitivity ECL detection kit (HY-K1005).

  • VGLUT1 Antibody (YA6880) Product Image
    1/16
  • Experimental Validation Results for VGLUT1 Antibody (YA6880)
  • VGLUT1 Antibody (YA6880) Product Image
  • Experimental Validation Results for VGLUT1 Antibody (YA6880)
    1/1
    MedChemExpress Validation
     Western blot analysis was performed on protein extracts (30 μg) from SH-SY5Y (lane 2), HeLa (lane 3), HepG2 (lane 4), and A549 (lane 5) using VGLUT1 antibody. Proteins were transferred onto a 0.45 μm PVDF membrane using the Trans-Blot® Turbo™ system for 13 min. The membrane was then blocked with 5% nonfat milk in TBST (HY-K1025) for 1 h at room temperature. Thhe primary antibody (1:2000) and loading control antibody GAPDH Antibody (HRP) (HY-P80954A) (1:5000) were diluted in 5% nonfat milk in TBST and incubated with the membrane overnight at 4°C. After washing, the membrane of primary antibody was incubated with HRP-conjugated goat anti-rabbit/mouse IgG secondary antibody (HY-P8001/HY-P8004) (1:5000) diluted in 5% nonfat milk in TBST for 1 h at room temperature. Protein bands were visualized using an Ultra High Sensitivity ECL detection kit (HY-K1005).
  • Experimental Validation Results for VGLUT1 Antibody (YA6880)
  • VGLUT1 Antibody (YA6880) Product Image
    MedChemExpress Validation

    Western blot analysis was performed on protein extracts (30 μg) from SH-SY5Y (lane 2), HeLa (lane 3), HepG2 (lane 4), and A549 (lane 5) using VGLUT1 antibody. Proteins were transferred onto a 0.45 μm PVDF membrane using the Trans-Blot® Turbo™ system for 13 min. The membrane was then blocked with 5% nonfat milk in TBST (HY-K1025) for 1 h at room temperature. Thhe primary antibody (1:2000) and loading control antibody GAPDH Antibody (HRP) (HY-P80954A) (1:5000) were diluted in 5% nonfat milk in TBST and incubated with the membrane overnight at 4°C. After washing, the membrane of primary antibody was incubated with HRP-conjugated goat anti-rabbit/mouse IgG secondary antibody (HY-P8001/HY-P8004) (1:5000) diluted in 5% nonfat milk in TBST for 1 h at room temperature. Protein bands were visualized using an Ultra High Sensitivity ECL detection kit (HY-K1005).

  • VGLUT1 Antibody (YA6880) Product Image WB