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P70 S6 Kinase beta Antibody (YA1514)

(Synonyms: RPS6KB2; STK14B; Ribosomal protein S6 kinase beta-2; S6K-beta-2; S6K2; 70 kDa ribosomal protein S6 kinase 2; P70S6K2; p70-S6K 2; S6 kinase-related kinase; SRK; Serine/threonine-protein kinase 14B; p70 ribosomal S6 kinase beta; S6K-beta; p70)
Customer Review

Based on 1 Customer Validation

P70 S6 Kinase beta Antibody (YA1514) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to P70 S6 Kinase beta.

For research use only. We do not sell to patients.
  • Host:

    Rabbit

  • Isotype:

    IgG

  • Application:

    WB, IHC-F, IHC-P, ICC/IF

  • Reactivity :

    Human, Mouse, Rat

  • Formulation:

    Supplied in 50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40% Glycerol, 0.01% Sodium azide and 0.05% BSA

  • Conjugation:
    Non-conjugated
ALL WB IHC-P mIHC ICC
  • Experimental Validation Results for P70 S6 Kinase beta Antibody (YA1514)
  • Experimental Validation Results for P70 S6 Kinase beta Antibody (YA1514)
  • Experimental Validation Results for P70 S6 Kinase beta Antibody (YA1514)
  • Experimental Validation Results for P70 S6 Kinase beta Antibody (YA1514)
  • Experimental Validation Results for P70 S6 Kinase beta Antibody (YA1514)
  • Experimental Validation Results for P70 S6 Kinase beta Antibody (YA1514)
  • Experimental Validation Results for P70 S6 Kinase beta Antibody (YA1514)
  • Experimental Validation Results for P70 S6 Kinase beta Antibody (YA1514)
  • Experimental Validation Results for P70 S6 Kinase beta Antibody (YA1514)
  • P70 S6 Kinase beta Antibody (YA1514) Product Image
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To place orders, for customer services and technical support, please contact: MedChemExpress USA

Tel: 609-228-6898 E-mail: [email protected] [email protected]

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Related Products:
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Use of MCE Products

Applications

Application
WB Info
WB: Western Blot
IHC-P Info
IHC-P: Immunohistochemistry-Paraffin
IHC-F Info
IHC-F: Immunohistochemistry-Frozen
ICC/IF Info
ICC/IF: Immunocytochemistry/
Immunofluorescence
Dilution Ratio 1:500-1:1000 1:50-1:100 1:50-1:100 1:50-1:200

Product Details

Description

P70 S6 Kinase beta Antibody (YA1514) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to P70 S6 Kinase beta.

  • Host Rabbit
  • Clonality Recombinant,Monoclonal
  • Species Reactivity
    Human, Mouse, Rat
  • Observed Molecular Weight
    Observed band size: 53 kDa Info
    Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.
  • Calculated Molecular Weight Predicted band size: 53 kDa
Species Reactivity Database
Immunogen

Recombinant protein of human P70 S6 Kinase beta aa1-120.

Sensitivity

Endogenous

Purification

Affinity Purified

Conjugation

Non-conjugated

Modification

Unmodified

Isotype

IgG

RRID

AB_3103875

Product Properties

  • Appearance

    Liquid

  • Formulation

    Supplied in 50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40% Glycerol, 0.01% Sodium azide and 0.05% BSA

  • Concentration

    Batch-dependent, Please check the COA for the concentration of each lot. Check Lot Concentration

  • Storage & Stability

    Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

  • Shipping

    Shipping with blue ice.

Verification Images

  • Experimental Validation Results for P70 S6 Kinase beta Antibody (YA1514)
    Western blot analysis was performed on extracts from Jurkat (lane 1, 15 μg), K562 (lane 2, 15 μg), MCF-7 (lane 3, 15 μg), 293 (lane 4, 15 μg), Hela (lane 5, 15 μg), and C6 (lane 6, 15 μg) using P70 S6 Kinase beta/SRK Rabbit mAb.Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST at 4°C overnight.The primary antibody (1:1000 dilution) and the loading control antibody (beta-Actin, HY-P80438, 1:20000 dilution) were incubated in 5% non-fat milk in TBST for 1 hour at 37°C.Goat Anti-Rabbit IgG-HRP Secondary Antibody (1:20000 dilution) was then applied for 40 minutes at 37°C.
  • Experimental Validation Results for P70 S6 Kinase beta Antibody (YA1514)
    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue using P70 S6 Kinase beta Antibody (YA1514). The section was pre-treated using heat mediated antigen retrieval with Tris/EDTA buffer (pH 9.0) for 20 minutes. The tissues were probed with the primary antibody (HY-P81769, 1/100) overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with neutral balsam.
  • Experimental Validation Results for P70 S6 Kinase beta Antibody (YA1514)
    Immunohistochemical analysis of paraffin-embedded human ovarian cancer tissue using P70 S6 Kinase beta Antibody (YA1514). The section was pre-treated using heat mediated antigen retrieval with Tris/EDTA buffer (pH 9.0) for 20 minutes. The tissues were probed with the primary antibody (HY-P81769, 1/100) overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with neutral balsam.
  • Experimental Validation Results for P70 S6 Kinase beta Antibody (YA1514)
    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue using P70 S6 Kinase beta Antibody (YA1514). The section was pre-treated using heat mediated antigen retrieval with Tris/EDTA buffer (pH 9.0) for 20 minutes. The tissues were probed with the primary antibody (HY-P81769, 1/100) overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with neutral balsam.
  • Experimental Validation Results for P70 S6 Kinase beta Antibody (YA1514)
    Tyramide signaling amplification based immunofluorescence analysis of paraffin-embedded human cervical cancer tissue using P70 S6 Kinase beta Antibody (YA1514) . The section was pre-treated using heat mediated antigen retrieval with Tris/EDTA buffer (pH 9.0) for 20 minutes. The tissues were probed with the primary antibody (HY-P81769, 1/300) overnight at 4℃. The detection was performed using an HRP conjugated compact polymer and fluorescent tyramide signal amplification system.Immunostaining was performed with Vari Fluor 594 TSA (200×)（HY-D1835）. Tissues were counterstained with DAPI (blue) and mounted with Anti-fade fluorescence mounting medium.
  • Experimental Validation Results for P70 S6 Kinase beta Antibody (YA1514)
    Tyramide signaling amplification based immunofluorescence analysis of paraffin-embedded human liver cancer tissue using P70 S6 Kinase beta Antibody (YA1514) . The section was pre-treated using heat mediated antigen retrieval with Tris/EDTA buffer (pH 9.0) for 20 minutes. The tissues were probed with the primary antibody (HY-P81769, 1/300) overnight at 4℃. The detection was performed using an HRP conjugated compact polymer and fluorescent tyramide signal amplification system.Immunostaining was performed with Vari Fluor 594 TSA (200×)（HY-D1835）. Tissues were counterstained with DAPI (blue) and mounted with Anti-fade fluorescence mounting medium.
  • Experimental Validation Results for P70 S6 Kinase beta Antibody (YA1514)
    Tyramide signaling amplification based immunofluorescence analysis of paraffin-embedded human colon cancer tissue using P70 S6 Kinase beta Antibody (YA1514) . The section was pre-treated using heat mediated antigen retrieval with Tris/EDTA buffer (pH 9.0) for 20 minutes. The tissues were probed with the primary antibody (HY-P81769, 1/300) overnight at 4℃. The detection was performed using an HRP conjugated compact polymer and fluorescent tyramide signal amplification system.Immunostaining was performed with Vari Fluor 594 TSA (200×)（HY-D1835）. Tissues were counterstained with DAPI (blue) and mounted with Anti-fade fluorescence mounting medium.
  • Experimental Validation Results for P70 S6 Kinase beta Antibody (YA1514)
    Immunocytochemistry analysis of Hela cells labeling P70 S6 Kinase beta with P70 S6 Kinase beta Antibody (HY-P81769) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with quick block buffer for 10 minutes at room temperature. Cells were then incubated with P70 S6 Kinase beta Antibody (HY-P81769) at 1/100 dilution in quick block buffer overnight at 4 ℃. AF488-conjugated Goat Anti-Rabbit IgG H&L (HY-P8002, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).
  • Experimental Validation Results for P70 S6 Kinase beta Antibody (YA1514)
    Immunocytochemistry analysis of PC-3 cells labeling P70 S6 Kinase beta with P70 S6 Kinase beta Antibody (HY-P81769) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with quick block buffer for 10 minutes at room temperature. Cells were then incubated with P70 S6 Kinase beta Antibody (HY-P81769) at 1/100 dilution in quick block buffer overnight at 4 ℃. AF488-conjugated Goat Anti-Rabbit IgG H&L (HY-P8002, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).

Background

  • Function

    P70 S6 Kinase beta phosphorylates specifically ribosomal protein S6. Seems to act downstream of mTOR signaling in response to growth factors and nutrients to promote cell proliferation, cell growth and cell cycle progression in an alternative pathway regulated by MEAK7[1].

  • Subcellular Localization

    Cytoplasm; Nucleus

  • Isoforms & Post-Translational Modification

    Q9UBS0 has 2 isomers: Q9UBS0-1: 53455 Da (predicted); Q9UBS0-2: 16868 Da (predicted).
    Phosphorylated and activated by MTOR. Phosphorylation by PKC within the NLS in response to mitogenic stimuli causes cytoplasmic retention

  • SwissProt ID

    Q9UBS0

  • Gene ID
    6199 [NCBI]
  • Synonyms

    RPS6KB2; STK14B; Ribosomal protein S6 kinase beta-2; S6K-beta-2; S6K2; 70 kDa ribosomal protein S6 kinase 2; P70S6K2; p70-S6K 2; S6 kinase-related kinase; SRK; Serine/threonine-protein kinase 14B; p70 ribosomal S6 kinase beta; S6K-beta; p70

  • Research Field

    Signal Transduction

P70 S6 Kinase beta Antibody (YA1514) Related Classifications

MOQ
Minimum order quantity
100 mg

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Measured by its ability to inhibit anti-CD3-induced proliferation of stimulated CTLL-2 mouse cytotoxic T cells. The ED50 this effect is 0.8023 μg/ml, corresponding to a specific activity is 1246.42 units/mg.

Assay Types
  • 1/16

    MedchemExpress Validation
     Western blot analysis was performed on extracts from Jurkat (lane 1, 15 μg), K562 (lane 2, 15 μg), MCF-7 (lane 3, 15 μg), 293 (lane 4, 15 μg), Hela (lane 5, 15 μg), and C6 (lane 6, 15 μg) using P70 S6 Kinase beta/SRK Rabbit mAb.Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST at 4°C overnight.The primary antibody (1:1000 dilution) and the loading control antibody (beta-Actin, HY-P80438, 1:20000 dilution) were incubated in 5% non-fat milk in TBST for 1 hour at 37°C.Goat Anti-Rabbit IgG-HRP Secondary Antibody (1:20000 dilution) was then applied for 40 minutes at 37°C.

  • 1/16

    MedchemExpress Validation
     Immunohistochemical analysis of paraffin-embedded human colon cancer tissue using P70 S6 Kinase beta Antibody (YA1514). The section was pre-treated using heat mediated antigen retrieval with Tris/EDTA buffer (pH 9.0) for 20 minutes. The tissues were probed with the primary antibody (HY-P81769, 1/100) overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with neutral balsam.

  • 1/16

    MedchemExpress Validation
     Immunohistochemical analysis of paraffin-embedded human ovarian cancer tissue using P70 S6 Kinase beta Antibody (YA1514). The section was pre-treated using heat mediated antigen retrieval with Tris/EDTA buffer (pH 9.0) for 20 minutes. The tissues were probed with the primary antibody (HY-P81769, 1/100) overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with neutral balsam.

  • 1/16

    MedchemExpress Validation
     Immunohistochemical analysis of paraffin-embedded human liver cancer tissue using P70 S6 Kinase beta Antibody (YA1514). The section was pre-treated using heat mediated antigen retrieval with Tris/EDTA buffer (pH 9.0) for 20 minutes. The tissues were probed with the primary antibody (HY-P81769, 1/100) overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with neutral balsam.

  • 1/16

    MedchemExpress Validation
     Tyramide signaling amplification based immunofluorescence analysis of paraffin-embedded human cervical cancer tissue using P70 S6 Kinase beta Antibody (YA1514) . The section was pre-treated using heat mediated antigen retrieval with Tris/EDTA buffer (pH 9.0) for 20 minutes. The tissues were probed with the primary antibody (HY-P81769, 1/300) overnight at 4℃. The detection was performed using an HRP conjugated compact polymer and fluorescent tyramide signal amplification system.Immunostaining was performed with Vari Fluor 594 TSA (200×)（HY-D1835）. Tissues were counterstained with DAPI (blue) and mounted with Anti-fade fluorescence mounting medium.

  • 1/16

    MedchemExpress Validation
     Tyramide signaling amplification based immunofluorescence analysis of paraffin-embedded human liver cancer tissue using P70 S6 Kinase beta Antibody (YA1514) . The section was pre-treated using heat mediated antigen retrieval with Tris/EDTA buffer (pH 9.0) for 20 minutes. The tissues were probed with the primary antibody (HY-P81769, 1/300) overnight at 4℃. The detection was performed using an HRP conjugated compact polymer and fluorescent tyramide signal amplification system.Immunostaining was performed with Vari Fluor 594 TSA (200×)（HY-D1835）. Tissues were counterstained with DAPI (blue) and mounted with Anti-fade fluorescence mounting medium.

  • 1/16

    MedchemExpress Validation
     Tyramide signaling amplification based immunofluorescence analysis of paraffin-embedded human colon cancer tissue using P70 S6 Kinase beta Antibody (YA1514) . The section was pre-treated using heat mediated antigen retrieval with Tris/EDTA buffer (pH 9.0) for 20 minutes. The tissues were probed with the primary antibody (HY-P81769, 1/300) overnight at 4℃. The detection was performed using an HRP conjugated compact polymer and fluorescent tyramide signal amplification system.Immunostaining was performed with Vari Fluor 594 TSA (200×)（HY-D1835）. Tissues were counterstained with DAPI (blue) and mounted with Anti-fade fluorescence mounting medium.

  • 1/16

    MedChemExpress Validation
     Immunocytochemistry analysis of Hela cells labeling P70 S6 Kinase beta with P70 S6 Kinase beta Antibody (HY-P81769) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with quick block buffer for 10 minutes at room temperature. Cells were then incubated with P70 S6 Kinase beta Antibody (HY-P81769) at 1/100 dilution in quick block buffer overnight at 4 ℃. AF488-conjugated Goat Anti-Rabbit IgG H&L (HY-P8002, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).

  • 1/16

    MedChemExpress Validation
     Immunocytochemistry analysis of PC-3 cells labeling P70 S6 Kinase beta with P70 S6 Kinase beta Antibody (HY-P81769) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with quick block buffer for 10 minutes at room temperature. Cells were then incubated with P70 S6 Kinase beta Antibody (HY-P81769) at 1/100 dilution in quick block buffer overnight at 4 ℃. AF488-conjugated Goat Anti-Rabbit IgG H&L (HY-P8002, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).

  • P70 S6 Kinase beta Antibody (YA1514) Product Image
    1/16
  • Experimental Validation Results for P70 S6 Kinase beta Antibody (YA1514)
  • Experimental Validation Results for P70 S6 Kinase beta Antibody (YA1514)
  • Experimental Validation Results for P70 S6 Kinase beta Antibody (YA1514)
  • Experimental Validation Results for P70 S6 Kinase beta Antibody (YA1514)
  • Experimental Validation Results for P70 S6 Kinase beta Antibody (YA1514)
  • Experimental Validation Results for P70 S6 Kinase beta Antibody (YA1514)
  • Experimental Validation Results for P70 S6 Kinase beta Antibody (YA1514)
  • Experimental Validation Results for P70 S6 Kinase beta Antibody (YA1514)
  • Experimental Validation Results for P70 S6 Kinase beta Antibody (YA1514)
  • P70 S6 Kinase beta Antibody (YA1514) Product Image
  • Experimental Validation Results for P70 S6 Kinase beta Antibody (YA1514)
    1/1
    MedchemExpress Validation
     Western blot analysis was performed on extracts from Jurkat (lane 1, 15 μg), K562 (lane 2, 15 μg), MCF-7 (lane 3, 15 μg), 293 (lane 4, 15 μg), Hela (lane 5, 15 μg), and C6 (lane 6, 15 μg) using P70 S6 Kinase beta/SRK Rabbit mAb.Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST at 4°C overnight.The primary antibody (1:1000 dilution) and the loading control antibody (beta-Actin, HY-P80438, 1:20000 dilution) were incubated in 5% non-fat milk in TBST for 1 hour at 37°C.Goat Anti-Rabbit IgG-HRP Secondary Antibody (1:20000 dilution) was then applied for 40 minutes at 37°C.
  • Experimental Validation Results for P70 S6 Kinase beta Antibody (YA1514)
    1/1
    MedchemExpress Validation
     Immunohistochemical analysis of paraffin-embedded human colon cancer tissue using P70 S6 Kinase beta Antibody (YA1514). The section was pre-treated using heat mediated antigen retrieval with Tris/EDTA buffer (pH 9.0) for 20 minutes. The tissues were probed with the primary antibody (HY-P81769, 1/100) overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with neutral balsam.
  • Experimental Validation Results for P70 S6 Kinase beta Antibody (YA1514)
    1/1
    MedchemExpress Validation
     Immunohistochemical analysis of paraffin-embedded human ovarian cancer tissue using P70 S6 Kinase beta Antibody (YA1514). The section was pre-treated using heat mediated antigen retrieval with Tris/EDTA buffer (pH 9.0) for 20 minutes. The tissues were probed with the primary antibody (HY-P81769, 1/100) overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with neutral balsam.
  • Experimental Validation Results for P70 S6 Kinase beta Antibody (YA1514)
    1/1
    MedchemExpress Validation
     Immunohistochemical analysis of paraffin-embedded human liver cancer tissue using P70 S6 Kinase beta Antibody (YA1514). The section was pre-treated using heat mediated antigen retrieval with Tris/EDTA buffer (pH 9.0) for 20 minutes. The tissues were probed with the primary antibody (HY-P81769, 1/100) overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with neutral balsam.
  • Experimental Validation Results for P70 S6 Kinase beta Antibody (YA1514)
    1/1
    MedchemExpress Validation
     Tyramide signaling amplification based immunofluorescence analysis of paraffin-embedded human cervical cancer tissue using P70 S6 Kinase beta Antibody (YA1514) . The section was pre-treated using heat mediated antigen retrieval with Tris/EDTA buffer (pH 9.0) for 20 minutes. The tissues were probed with the primary antibody (HY-P81769, 1/300) overnight at 4℃. The detection was performed using an HRP conjugated compact polymer and fluorescent tyramide signal amplification system.Immunostaining was performed with Vari Fluor 594 TSA (200×)（HY-D1835）. Tissues were counterstained with DAPI (blue) and mounted with Anti-fade fluorescence mounting medium.
  • Experimental Validation Results for P70 S6 Kinase beta Antibody (YA1514)
    1/1
    MedchemExpress Validation
     Tyramide signaling amplification based immunofluorescence analysis of paraffin-embedded human liver cancer tissue using P70 S6 Kinase beta Antibody (YA1514) . The section was pre-treated using heat mediated antigen retrieval with Tris/EDTA buffer (pH 9.0) for 20 minutes. The tissues were probed with the primary antibody (HY-P81769, 1/300) overnight at 4℃. The detection was performed using an HRP conjugated compact polymer and fluorescent tyramide signal amplification system.Immunostaining was performed with Vari Fluor 594 TSA (200×)（HY-D1835）. Tissues were counterstained with DAPI (blue) and mounted with Anti-fade fluorescence mounting medium.
  • Experimental Validation Results for P70 S6 Kinase beta Antibody (YA1514)
    1/1
    MedchemExpress Validation
     Tyramide signaling amplification based immunofluorescence analysis of paraffin-embedded human colon cancer tissue using P70 S6 Kinase beta Antibody (YA1514) . The section was pre-treated using heat mediated antigen retrieval with Tris/EDTA buffer (pH 9.0) for 20 minutes. The tissues were probed with the primary antibody (HY-P81769, 1/300) overnight at 4℃. The detection was performed using an HRP conjugated compact polymer and fluorescent tyramide signal amplification system.Immunostaining was performed with Vari Fluor 594 TSA (200×)（HY-D1835）. Tissues were counterstained with DAPI (blue) and mounted with Anti-fade fluorescence mounting medium.
  • Experimental Validation Results for P70 S6 Kinase beta Antibody (YA1514)
    1/1
    MedChemExpress Validation
     Immunocytochemistry analysis of Hela cells labeling P70 S6 Kinase beta with P70 S6 Kinase beta Antibody (HY-P81769) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with quick block buffer for 10 minutes at room temperature. Cells were then incubated with P70 S6 Kinase beta Antibody (HY-P81769) at 1/100 dilution in quick block buffer overnight at 4 ℃. AF488-conjugated Goat Anti-Rabbit IgG H&L (HY-P8002, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).
  • Experimental Validation Results for P70 S6 Kinase beta Antibody (YA1514)
    1/1
    MedChemExpress Validation
     Immunocytochemistry analysis of PC-3 cells labeling P70 S6 Kinase beta with P70 S6 Kinase beta Antibody (HY-P81769) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with quick block buffer for 10 minutes at room temperature. Cells were then incubated with P70 S6 Kinase beta Antibody (HY-P81769) at 1/100 dilution in quick block buffer overnight at 4 ℃. AF488-conjugated Goat Anti-Rabbit IgG H&L (HY-P8002, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).
  • Experimental Validation Results for P70 S6 Kinase beta Antibody (YA1514)
  • Experimental Validation Results for P70 S6 Kinase beta Antibody (YA1514)
  • Experimental Validation Results for P70 S6 Kinase beta Antibody (YA1514)
  • Experimental Validation Results for P70 S6 Kinase beta Antibody (YA1514)
  • Experimental Validation Results for P70 S6 Kinase beta Antibody (YA1514)
  • Experimental Validation Results for P70 S6 Kinase beta Antibody (YA1514)
  • Experimental Validation Results for P70 S6 Kinase beta Antibody (YA1514)
  • Experimental Validation Results for P70 S6 Kinase beta Antibody (YA1514)
  • Experimental Validation Results for P70 S6 Kinase beta Antibody (YA1514)
  • P70 S6 Kinase beta Antibody (YA1514) Product Image
    MedchemExpress Validation

    Western blot analysis was performed on extracts from Jurkat (lane 1, 15 μg), K562 (lane 2, 15 μg), MCF-7 (lane 3, 15 μg), 293 (lane 4, 15 μg), Hela (lane 5, 15 μg), and C6 (lane 6, 15 μg) using P70 S6 Kinase beta/SRK Rabbit mAb.Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST at 4°C overnight.The primary antibody (1:1000 dilution) and the loading control antibody (beta-Actin, HY-P80438, 1:20000 dilution) were incubated in 5% non-fat milk in TBST for 1 hour at 37°C.Goat Anti-Rabbit IgG-HRP Secondary Antibody (1:20000 dilution) was then applied for 40 minutes at 37°C.

  • P70 S6 Kinase beta Antibody (YA1514) Product Image
    MedchemExpress Validation

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue using P70 S6 Kinase beta Antibody (YA1514). The section was pre-treated using heat mediated antigen retrieval with Tris/EDTA buffer (pH 9.0) for 20 minutes. The tissues were probed with the primary antibody (HY-P81769, 1/100) overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with neutral balsam.

  • P70 S6 Kinase beta Antibody (YA1514) Product Image
    MedchemExpress Validation

    Immunohistochemical analysis of paraffin-embedded human ovarian cancer tissue using P70 S6 Kinase beta Antibody (YA1514). The section was pre-treated using heat mediated antigen retrieval with Tris/EDTA buffer (pH 9.0) for 20 minutes. The tissues were probed with the primary antibody (HY-P81769, 1/100) overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with neutral balsam.

  • P70 S6 Kinase beta Antibody (YA1514) Product Image
    MedchemExpress Validation

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue using P70 S6 Kinase beta Antibody (YA1514). The section was pre-treated using heat mediated antigen retrieval with Tris/EDTA buffer (pH 9.0) for 20 minutes. The tissues were probed with the primary antibody (HY-P81769, 1/100) overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with neutral balsam.

  • P70 S6 Kinase beta Antibody (YA1514) Product Image
    MedchemExpress Validation

    Tyramide signaling amplification based immunofluorescence analysis of paraffin-embedded human cervical cancer tissue using P70 S6 Kinase beta Antibody (YA1514) . The section was pre-treated using heat mediated antigen retrieval with Tris/EDTA buffer (pH 9.0) for 20 minutes. The tissues were probed with the primary antibody (HY-P81769, 1/300) overnight at 4℃. The detection was performed using an HRP conjugated compact polymer and fluorescent tyramide signal amplification system.Immunostaining was performed with Vari Fluor 594 TSA (200×)（HY-D1835）. Tissues were counterstained with DAPI (blue) and mounted with Anti-fade fluorescence mounting medium.

  • P70 S6 Kinase beta Antibody (YA1514) Product Image
    MedchemExpress Validation

    Tyramide signaling amplification based immunofluorescence analysis of paraffin-embedded human liver cancer tissue using P70 S6 Kinase beta Antibody (YA1514) . The section was pre-treated using heat mediated antigen retrieval with Tris/EDTA buffer (pH 9.0) for 20 minutes. The tissues were probed with the primary antibody (HY-P81769, 1/300) overnight at 4℃. The detection was performed using an HRP conjugated compact polymer and fluorescent tyramide signal amplification system.Immunostaining was performed with Vari Fluor 594 TSA (200×)（HY-D1835）. Tissues were counterstained with DAPI (blue) and mounted with Anti-fade fluorescence mounting medium.

  • P70 S6 Kinase beta Antibody (YA1514) Product Image
    MedchemExpress Validation

    Tyramide signaling amplification based immunofluorescence analysis of paraffin-embedded human colon cancer tissue using P70 S6 Kinase beta Antibody (YA1514) . The section was pre-treated using heat mediated antigen retrieval with Tris/EDTA buffer (pH 9.0) for 20 minutes. The tissues were probed with the primary antibody (HY-P81769, 1/300) overnight at 4℃. The detection was performed using an HRP conjugated compact polymer and fluorescent tyramide signal amplification system.Immunostaining was performed with Vari Fluor 594 TSA (200×)（HY-D1835）. Tissues were counterstained with DAPI (blue) and mounted with Anti-fade fluorescence mounting medium.

  • P70 S6 Kinase beta Antibody (YA1514) Product Image
    MedChemExpress Validation

    Immunocytochemistry analysis of Hela cells labeling P70 S6 Kinase beta with P70 S6 Kinase beta Antibody (HY-P81769) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with quick block buffer for 10 minutes at room temperature. Cells were then incubated with P70 S6 Kinase beta Antibody (HY-P81769) at 1/100 dilution in quick block buffer overnight at 4 ℃. AF488-conjugated Goat Anti-Rabbit IgG H&L (HY-P8002, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).

  • P70 S6 Kinase beta Antibody (YA1514) Product Image
    MedChemExpress Validation

    Immunocytochemistry analysis of PC-3 cells labeling P70 S6 Kinase beta with P70 S6 Kinase beta Antibody (HY-P81769) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with quick block buffer for 10 minutes at room temperature. Cells were then incubated with P70 S6 Kinase beta Antibody (HY-P81769) at 1/100 dilution in quick block buffer overnight at 4 ℃. AF488-conjugated Goat Anti-Rabbit IgG H&L (HY-P8002, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).

  • P70 S6 Kinase beta Antibody (YA1514) Product Image WB
  • P70 S6 Kinase beta Antibody (YA1514) Product Image IHC-P
  • P70 S6 Kinase beta Antibody (YA1514) Product Image IHC-P
  • P70 S6 Kinase beta Antibody (YA1514) Product Image IHC-P
  • P70 S6 Kinase beta Antibody (YA1514) Product Image mIHC
  • P70 S6 Kinase beta Antibody (YA1514) Product Image mIHC
  • P70 S6 Kinase beta Antibody (YA1514) Product Image mIHC
  • P70 S6 Kinase beta Antibody (YA1514) Product Image ICC
  • P70 S6 Kinase beta Antibody (YA1514) Product Image ICC