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Rab3A Antibody (YA6964)

(Synonyms: Rab 3A antibody; RAB 3A member RAS oncogene family antibody; Rab3a antibody; RAB3A member RAS oncogene family antibody; RAB3A_HUMAN antibody; RAS associated protein RAB 3A antibody; RAS associated protein RAB3A antibody; Ras related protein Rab 3A antibody; Ras related protein Rab3A antibody; Ras-related protein Rab-3A antibody)
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Based on 1 Customer Validation

Rab3A Antibody (YA6964) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to Rab3A.

For research use only. We do not sell to patients.
  • Host:

    Rabbit

  • Isotype:

    IgG

  • Application:

    WB, IHC-P, ICC/IF, FC

  • Reactivity :

    Human, Mouse, Rat

  • Formulation:

    Supplied in PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

  • Conjugation:
    Non-conjugated
  • Experimental Validation Results for Rab3A Antibody (YA6964)
  • Rab3A Antibody (YA6964) Product Image
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To place orders, for customer services and technical support, please contact: MedChemExpress USA

Tel: 609-228-6898 E-mail: [email protected] [email protected]

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Related Products:
Top Publications Citing

Use of MCE Products

Applications

Application
WB Info
WB: Western Blot
IHC-P Info
IHC-P: Immunohistochemistry-Paraffin
ICC/IF Info
ICC/IF: Immunocytochemistry/
Immunofluorescence
FC Info
FC: Flow Cytometry
Dilution Ratio 1:5000 1:1000 1:100 1:500-1:1000

Product Details

Description

Rab3A Antibody (YA6964) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to Rab3A.

  • Host Rabbit
  • Clonality Recombinant,Monoclonal
  • Species Reactivity
    Human, Mouse, Rat
  • Observed Molecular Weight
    Observed band size: 25 kDa Info
    Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.
  • Calculated Molecular Weight Predicted band size: 25 kDa
Immunogen

Synthetic peptide within human Rab3A aa 121-170 / 220.

Sensitivity

Endogenous

Purification

Protein A affinity purified.

Conjugation

Non-conjugated

Modification

Unmodified

Isotype

IgG

Product Properties

  • Appearance

    Liquid

  • Formulation

    Supplied in PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

  • Concentration

    Batch-dependent, Please check the COA for the concentration of each lot. Check Lot Concentration

  • Storage & Stability

    Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

  • Shipping

    Shipping with blue ice.

Verification Images

  • Experimental Validation Results for Rab3A Antibody (YA6964)
    Western blot analysis of extracts from SH-SY5Y (lane 1(20μg)) 、U87-MG (lane 2(20μg)) 、Mouse brain (lane 3(20μg)) and Rat brain (lane 4(20μg)) using Rab3A Antibody (HY-P87281) . Proteins were transferred to a PVDF membrane and blocked with 5% nonfat dry milk in TBST for 1.5 hour at room temperature. The primary antibody (1/5000) and Loading control antibody (β Tubulin, 1/10000) was used in 5% nonfat dry milk in TBST at 4℃ overnight. Goat Anti-Rabbit IgG-HRP Secondary Antibody (1/10,000) was used for 1 hour at room temperature.

Background

  • Function

    Rab3A is a The small GTPases Rab are key regulators of intracellular membrane trafficking, from the formation of transport vesicles to their fusion with membranes. Rabs cycle between an inactive GDP-bound form and an active GTP-bound form that is able to recruit to membranes different sets of downstream effectors directly responsible for vesicle formation, movement, tethering and fusion. RAB3A plays a central role in regulated exocytosis and secretion. Controls the recruitment, tethering and docking of secretory vesicles to the plasma membrane. Upon stimulation, switches to its active GTP-bound form, cycles to vesicles and recruits effectors such as RIMS1, RIMS2, Rabphilin-3A/RPH3A, RPH3AL or SYTL4 to help the docking of vesicules onto the plasma membrane. Upon GTP hydrolysis by GTPase-activating protein, dissociates from the vesicle membrane allowing the exocytosis to proceed. Stimulates insulin secretion through interaction with RIMS2 or RPH3AL effectors in pancreatic beta cells. Regulates calcium-dependent lysosome exocytosis and plasma membrane repair (PMR) via the interaction with 2 effectors, SYTL4 and myosin-9/MYH9. Acts as a positive regulator of acrosome content secretion in sperm cells by interacting with RIMS1. Also plays a role in the regulation of dopamine release by interacting with synaptotagmin I/SYT[1][2][3][4].

  • Subcellular Localization

    Cytoplasm, cytosol,Lysosome,Cytoplasmic vesicle, secretory vesicle,Cell projection, axon,Cell membrane,Presynapse,Postsynapse

  • Expression


    Tissue_Specificity: Specifically expressed in brain

  • Isoforms & Post-Translational Modification

    P20336: 220 amino acids, molecular weight 24984 Da.

  • Subunit

    Interacts with RIMS1 and RIMS2 (By similarity)

  • SwissProt ID

    P20336

  • Synonyms

    Rab 3A antibody; RAB 3A member RAS oncogene family antibody; Rab3a antibody; RAB3A member RAS oncogene family antibody; RAB3A_HUMAN antibody; RAS associated protein RAB 3A antibody; RAS associated protein RAB3A antibody; Ras related protein Rab 3A antibody; Ras related protein Rab3A antibody; Ras-related protein Rab-3A antibody

Rab3A Antibody (YA6964) Related Classifications

MOQ
Minimum order quantity
100 mg

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Measured by its ability to inhibit anti-CD3-induced proliferation of stimulated CTLL-2 mouse cytotoxic T cells. The ED50 this effect is 0.8023 μg/ml, corresponding to a specific activity is 1246.42 units/mg.

Assay Types
  • 1/16

    MedChemExpress Validation
     Western blot analysis of extracts from SH-SY5Y (lane 1(20μg)) 、U87-MG (lane 2(20μg)) 、Mouse brain (lane 3(20μg)) and Rat brain (lane 4(20μg)) using Rab3A Antibody (HY-P87281) . Proteins were transferred to a PVDF membrane and blocked with 5% nonfat dry milk in TBST for 1.5 hour at room temperature. The primary antibody (1/5000) and Loading control antibody (β Tubulin, 1/10000) was used in 5% nonfat dry milk in TBST at 4℃ overnight. Goat Anti-Rabbit IgG-HRP Secondary Antibody (1/10,000) was used for 1 hour at room temperature.

  • Rab3A Antibody (YA6964) Product Image
    1/16
  • Experimental Validation Results for Rab3A Antibody (YA6964)
  • Rab3A Antibody (YA6964) Product Image
  • Experimental Validation Results for Rab3A Antibody (YA6964)
    1/1
    MedChemExpress Validation
     Western blot analysis of extracts from SH-SY5Y (lane 1(20μg)) 、U87-MG (lane 2(20μg)) 、Mouse brain (lane 3(20μg)) and Rat brain (lane 4(20μg)) using Rab3A Antibody (HY-P87281) . Proteins were transferred to a PVDF membrane and blocked with 5% nonfat dry milk in TBST for 1.5 hour at room temperature. The primary antibody (1/5000) and Loading control antibody (β Tubulin, 1/10000) was used in 5% nonfat dry milk in TBST at 4℃ overnight. Goat Anti-Rabbit IgG-HRP Secondary Antibody (1/10,000) was used for 1 hour at room temperature.
  • Experimental Validation Results for Rab3A Antibody (YA6964)
  • Rab3A Antibody (YA6964) Product Image
    MedChemExpress Validation

    Western blot analysis of extracts from SH-SY5Y (lane 1(20μg)) 、U87-MG (lane 2(20μg)) 、Mouse brain (lane 3(20μg)) and Rat brain (lane 4(20μg)) using Rab3A Antibody (HY-P87281) . Proteins were transferred to a PVDF membrane and blocked with 5% nonfat dry milk in TBST for 1.5 hour at room temperature. The primary antibody (1/5000) and Loading control antibody (β Tubulin, 1/10000) was used in 5% nonfat dry milk in TBST at 4℃ overnight. Goat Anti-Rabbit IgG-HRP Secondary Antibody (1/10,000) was used for 1 hour at room temperature.

  • Rab3A Antibody (YA6964) Product Image WB