SPIB Antibody (YA4161)(PBS only)

Cat. No.: HY-P84464A: Data Sheet User Guide for Antibodies
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SPIB Antibody (YA4161) is a Mouse-derived and non-conjugated IgG1 monoclonal antibody, targeting to SPIB.

For research use only. We do not sell to patients.

Size		Stock
50 μL		Get quote
100 μL		Get quote

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WB: Western Blot;
IHC-P: Immunohistochemistry-Paraffin;
IHC-F: Immunohistochemistry-Frozen;
ICC/IF: Immunocytochemistry/Immunofluorescence;
IF-Tissue: Immunofluorescence-Tissue;
mIHC: Multiplex Immunohistochemical;
IP: Immunoprecipitation;
ChIP: Chromatin Immunoprecipitation;
FC: Flow Cytometry;
ELISA: Enzyme Linked Immunosorbent Assay

Product Detail
Background
Documentation

Description

SPIB Antibody (YA4161) is a Mouse-derived and non-conjugated IgG1 monoclonal antibody, targeting to SPIB.

Host

Mouse

Molecular Weight

Predicted band size: 28.8 kDa;

Observed band size: 29 kDa

Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.

Species Reactivity

Human, Mouse

SwissProt ID

Q01892

Gene ID

6689 [NCBI]

Immunogen

Purified recombinant fragment of human SPIB (AA: 200-252) expressed in E. Coli.

Application &
Dilution Ratio

Application	Dilution Ratio
WB WB: Western Blot	1:500-1:2000
IHC-P IHC-P: Immunohistochemistry-Paraffin	1:200-1:1000
ICC/IF ICC/IF: Immunocytochemistry/Immunofluorescence	1:200-1:1000
FC FC: Flow Cytometry	1:200-1:400
ELISA ELISA: Enzyme Linked Immunosorbent Assay	1:10000

Purity	affinity purified.	Conjugation	Non-conjugated
Isotype	IgG1

Appearance

Liquid

Formulation

Supplied in PBS, pH 7.4.

Storage & Stability

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

Shipping

Shipping with blue ice.

Background

Function：Sequence specific transcriptional activator which binds to the PU-box, a purine-rich DNA sequence (5'-GAGGAA-3') that can act as a lymphoid-specific enhancer. Promotes development of plasmacytoid dendritic cells (pDCs), also known as type 2 DC precursors (pre-DC2) or natural interferon (IFN)-producing cells. These cells have the capacity to produce large amounts of interferon and block viral replication. May be required for B-cell receptor (BCR) signaling, which is necessary for normal B-cell development and antigenic stimulation

Subcellular Localization：Nucleus; Cytoplasm

Expression：
Tissue_specificity:Expressed in plasmacytoid dendritic cells (pDCs) and B-cells, not expressed in T-cells or granulocytes. May also be enriched in stem cell populations of the liver

Subunit：Can form homotypic interactions (PubMed:10196196). Interacts with IRF4/Pip (PubMed:10196196). Interacts with JUN (PubMed:10196196). Interacts with TBP (PubMed:10196196). May also interact with CREBBP and EP300 (PubMed:11864910). Interacts with NONO/p54(nrb) (By similarity)

Synonyms

SPI-B

Documentation

User Guide for Antibodies (1077 KB)