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PKA 2 beta (regulatory subunit)Antibody (YA133)

(Synonyms: cAMP-dependent protein kinase type II-beta regulatory subunit, PRKAR2B)
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Based on 1 Customer Validation

PKA 2 beta (regulatory subunit)Antibody (YA133) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to PKA 2 beta (regulatory subunit).

For research use only. We do not sell to patients.
  • Host:

    Rabbit

  • Isotype:

    IgG

  • Application:

    WB, IP, ICC/IF, IHC-P, FC

  • Reactivity :

    Human, Mouse, Rat

  • Formulation:

    Supplied in 1*TBS (pH7.4), 0.05% BSA and 40% Glycerol. Preservative: 0.05% Sodium Azide.

  • Conjugation:
    Non-conjugated
ALL WB IHC-P
  • Experimental Validation Results for PKA 2 beta (regulatory subunit)Antibody (YA133)
  • Experimental Validation Results for PKA 2 beta (regulatory subunit)Antibody (YA133)
  • Experimental Validation Results for PKA 2 beta (regulatory subunit)Antibody (YA133)
  • PKA 2 beta (regulatory subunit)Antibody (YA133) Product Image
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To place orders, for customer services and technical support, please contact: MedChemExpress USA

Tel: 609-228-6898 E-mail: [email protected] [email protected]

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Data Download
Related Products:
Top Publications Citing

Use of MCE Products

Applications

Application
WB Info
WB: Western Blot
ICC/IF Info
ICC/IF: Immunocytochemistry/
Immunofluorescence
IHC-P Info
IHC-P: Immunohistochemistry-Paraffin
FC Info
FC: Flow Cytometry
Dilution Ratio 1:500-1:1000 1:50-1:200 1:50-1:200 1:50-1:100

Product Details

Description

PKA 2 beta (regulatory subunit)Antibody (YA133) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to PKA 2 beta (regulatory subunit).

  • Host Rabbit
  • Clonality Recombinant,Monoclonal
  • Species Reactivity
    Human, Mouse, Rat
  • Observed Molecular Weight
    Observed band size: 50 kDa Info
    Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.
  • Calculated Molecular Weight Predicted band size: 46 kDa
Species Reactivity Database
Immunogen

Synthetic peptide corresponding to Human PKA 2 beta.AA range:369-418.

Sensitivity

Endogenous

Purification

Protein A affinity purified.

Conjugation

Non-conjugated

Modification

Unmodified

Isotype

IgG

RRID

AB_3102511

Product Properties

  • Appearance

    Solution

  • Formulation

    Supplied in 1*TBS (pH7.4), 0.05% BSA and 40% Glycerol. Preservative: 0.05% Sodium Azide.

  • Concentration

    Batch-dependent, Please check the COA for the concentration of each lot. Check Lot Concentration

  • Storage & Stability

    Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

  • Shipping

    Shipping with blue ice.

Verification Images

  • Experimental Validation Results for PKA 2 beta (regulatory subunit)Antibody (YA133)
    Western blot analysis of extracts from K562 (lane 2, 20ug), Rat brain (lane 3, 20ug), Mouse brain (lane 4, 20ug) and Hela (lane 5, 20ug) using PKA 2 beta Antibody (HY-P80428). Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST at 4°C overnight. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P80993, 1/10,000) was used in 5% non-fat milk in TBST for 2 hour at room temperature. Goat Anti-Rabbit IgG-HRP Secondary Antibody (HY-P8001, 1/10,000) was used for 1 hour at room temperature.
  • Experimental Validation Results for PKA 2 beta (regulatory subunit)Antibody (YA133)
    Immunohistochemical analysis of paraffin-embedded Rat brain tissue using PKA 2 beta (regulatory subunit)Antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 8 minutes. The tissues were blocked in QuickBlock for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HY-P80428, 1:400) in 4℃ overnight. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
  • Experimental Validation Results for PKA 2 beta (regulatory subunit)Antibody (YA133)
    Immunohistochemical analysis of paraffin-embedded Rat brain tissue using PKA 2 beta (regulatory subunit)Antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 8 minutes. The tissues were blocked in QuickBlock for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HY-P80428, 1:400) in 4℃ overnight. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Background

  • Function

    PKA 2 beta is a Regulatory subunit of the cAMP-dependent protein kinases involved in cAMP signaling in cells. Type II regulatory chains mediate membrane association by binding to anchoring proteins, including the MAP2 kinase

  • Subcellular Localization

    Cytoplasm; Cell membrane

  • Expression


    Tissue_specificity:Four types of regulatory chains are found: I-alpha, I-beta, II-alpha, and II-beta. Their expression varies among tissues and is in some cases constitutive and in others inducible

  • Subunit

    The inactive form of the enzyme is composed of two regulatory chains and two catalytic chains. Activation by cAMP produces two active catalytic monomers and a regulatory dimer that binds four cAMP molecules. Interacts with PRKACA and PRKACB (PubMed:33058759). Interacts with the phosphorylated form of PJA2. Forms a complex composed of PRKAR2B, GSK3B and GSKIP through GSKIP interaction; facilitates PKA-induced phosphorylation and regulates GSK3B activity (PubMed:25920809)

  • SwissProt ID

    P31323

  • Gene ID
    5577 [NCBI]
  • Synonyms

    cAMP-dependent protein kinase type II-beta regulatory subunit, PRKAR2B

  • Research Field

    Neuroscience

PKA 2 beta (regulatory subunit)Antibody (YA133) Related Classifications

MOQ
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100 mg

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Measured by its ability to inhibit anti-CD3-induced proliferation of stimulated CTLL-2 mouse cytotoxic T cells. The ED50 this effect is 0.8023 μg/ml, corresponding to a specific activity is 1246.42 units/mg.

Assay Types
  • 1/16

    MedChemExpress Validation
     Western blot analysis of extracts from K562 (lane 2, 20ug), Rat brain (lane 3, 20ug), Mouse brain (lane 4, 20ug) and Hela (lane 5, 20ug) using PKA 2 beta Antibody (HY-P80428). Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST at 4°C overnight. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P80993, 1/10,000) was used in 5% non-fat milk in TBST for 2 hour at room temperature. Goat Anti-Rabbit IgG-HRP Secondary Antibody (HY-P8001, 1/10,000) was used for 1 hour at room temperature.

  • 1/16

    MedchemExpress Validation
     Immunohistochemical analysis of paraffin-embedded Rat brain tissue using PKA 2 beta (regulatory subunit)Antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 8 minutes. The tissues were blocked in QuickBlock for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HY-P80428, 1:400) in 4℃ overnight. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • 1/16

    MedchemExpress Validation
     Immunohistochemical analysis of paraffin-embedded Rat brain tissue using PKA 2 beta (regulatory subunit)Antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 8 minutes. The tissues were blocked in QuickBlock for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HY-P80428, 1:400) in 4℃ overnight. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • PKA 2 beta (regulatory subunit)Antibody (YA133) Product Image
    1/16
  • Experimental Validation Results for PKA 2 beta (regulatory subunit)Antibody (YA133)
  • Experimental Validation Results for PKA 2 beta (regulatory subunit)Antibody (YA133)
  • Experimental Validation Results for PKA 2 beta (regulatory subunit)Antibody (YA133)
  • PKA 2 beta (regulatory subunit)Antibody (YA133) Product Image
  • Experimental Validation Results for PKA 2 beta (regulatory subunit)Antibody (YA133)
    1/1
    MedChemExpress Validation
     Western blot analysis of extracts from K562 (lane 2, 20ug), Rat brain (lane 3, 20ug), Mouse brain (lane 4, 20ug) and Hela (lane 5, 20ug) using PKA 2 beta Antibody (HY-P80428). Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST at 4°C overnight. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P80993, 1/10,000) was used in 5% non-fat milk in TBST for 2 hour at room temperature. Goat Anti-Rabbit IgG-HRP Secondary Antibody (HY-P8001, 1/10,000) was used for 1 hour at room temperature.
  • Experimental Validation Results for PKA 2 beta (regulatory subunit)Antibody (YA133)
    1/1
    MedchemExpress Validation
     Immunohistochemical analysis of paraffin-embedded Rat brain tissue using PKA 2 beta (regulatory subunit)Antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 8 minutes. The tissues were blocked in QuickBlock for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HY-P80428, 1:400) in 4℃ overnight. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
  • Experimental Validation Results for PKA 2 beta (regulatory subunit)Antibody (YA133)
    1/1
    MedchemExpress Validation
     Immunohistochemical analysis of paraffin-embedded Rat brain tissue using PKA 2 beta (regulatory subunit)Antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 8 minutes. The tissues were blocked in QuickBlock for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HY-P80428, 1:400) in 4℃ overnight. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
  • Experimental Validation Results for PKA 2 beta (regulatory subunit)Antibody (YA133)
  • Experimental Validation Results for PKA 2 beta (regulatory subunit)Antibody (YA133)
  • Experimental Validation Results for PKA 2 beta (regulatory subunit)Antibody (YA133)
  • PKA 2 beta (regulatory subunit)Antibody (YA133) Product Image
    MedChemExpress Validation

    Western blot analysis of extracts from K562 (lane 2, 20ug), Rat brain (lane 3, 20ug), Mouse brain (lane 4, 20ug) and Hela (lane 5, 20ug) using PKA 2 beta Antibody (HY-P80428). Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST at 4°C overnight. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P80993, 1/10,000) was used in 5% non-fat milk in TBST for 2 hour at room temperature. Goat Anti-Rabbit IgG-HRP Secondary Antibody (HY-P8001, 1/10,000) was used for 1 hour at room temperature.

  • PKA 2 beta (regulatory subunit)Antibody (YA133) Product Image
    MedchemExpress Validation

    Immunohistochemical analysis of paraffin-embedded Rat brain tissue using PKA 2 beta (regulatory subunit)Antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 8 minutes. The tissues were blocked in QuickBlock for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HY-P80428, 1:400) in 4℃ overnight. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • PKA 2 beta (regulatory subunit)Antibody (YA133) Product Image
    MedchemExpress Validation

    Immunohistochemical analysis of paraffin-embedded Rat brain tissue using PKA 2 beta (regulatory subunit)Antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 8 minutes. The tissues were blocked in QuickBlock for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HY-P80428, 1:400) in 4℃ overnight. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • PKA 2 beta (regulatory subunit)Antibody (YA133) Product Image WB
  • PKA 2 beta (regulatory subunit)Antibody (YA133) Product Image IHC-P
  • PKA 2 beta (regulatory subunit)Antibody (YA133) Product Image IHC-P