Semaphorin 7A Antibody (YA3554)(PBS only)

Cat. No.: HY-P83857A: Data Sheet User Guide for Antibodies
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Semaphorin 7A Antibody (YA3554) is a Mouse-derived and non-conjugated IgG1 monoclonal antibody, targeting to Semaphorin 7A.

For research use only. We do not sell to patients.

Size		Stock
50 μL		Get quote
100 μL		Get quote

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WB: Western Blot;
IHC-P: Immunohistochemistry-Paraffin;
IHC-F: Immunohistochemistry-Frozen;
ICC/IF: Immunocytochemistry/Immunofluorescence;
IF-Tissue: Immunofluorescence-Tissue;
mIHC: Multiplex Immunohistochemical;
IP: Immunoprecipitation;
ChIP: Chromatin Immunoprecipitation;
FC: Flow Cytometry;
ELISA: Enzyme Linked Immunosorbent Assay

Product Detail
Background
Documentation

Description

Semaphorin 7A Antibody (YA3554) is a Mouse-derived and non-conjugated IgG1 monoclonal antibody, targeting to Semaphorin 7A.

Host

Mouse

Molecular Weight

Predicted band size: 75 kDa;

Observed band size: 75 kDa

Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.

Species Reactivity

Human

SwissProt ID

O75326

Gene ID

8482 [NCBI]

Immunogen

Purified recombinant fragment of human ZAP70 (AA: 169-390) expressed in E. Coli.

Application &
Dilution Ratio

Application	Dilution Ratio
WB WB: Western Blot	1:500-1:2000
ELISA ELISA: Enzyme Linked Immunosorbent Assay	1:10000

Purity	affinity purified.	Conjugation	Non-conjugated
Isotype	IgG1

Appearance

Liquid

Formulation

Supplied in PBS, pH 7.4.

Storage & Stability

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

Shipping

Shipping with blue ice.

Background

Function：Plays an important role in integrin-mediated signaling and functions both in regulating cell migration and immune responses. Promotes formation of focal adhesion complexes, activation of the protein kinase PTK2/FAK1 and subsequent phosphorylation of MAPK1 and MAPK3. Promotes production of pro-inflammatory cytokines by monocytes and macrophages. Plays an important role in modulating inflammation and T-cell-mediated immune responses. Promotes axon growth in the embryonic olfactory bulb. Promotes attachment, spreading and dendrite outgrowth in melanocytes

Subcellular Localization：Cell membrane; Lipid-anchor, GPI-anchor; Extracellular side

Expression：
Tissue_specificity:Protein levels were detected in keratinocytes and vascular endothelial cells of the skin. It was also expressed in fibroblasts, keratinocytes, melanocytes, placenta, testes, ovaries, spleen, brain, spinal cord, lungs, heart, adrenal glands, lymph nodes, thymus, intestines, and kidneys.

Induction:Up-regulated in UV-irradiated fibroblasts, but not in UV-irradiated keratinocytes

Isoforms & Post-Translational Modification：O75326 has 2 isomers: O75326-1: 74824 Da (predicted); O75326-2: 73364 Da (predicted).

Subunit：Interacts with ITGA1 and ITGB1 (Probable). Interacts with PLXNC1

Synonyms

SEMA7A; JMH; SEMAL; CDw108; SEMAK1; H-Sema-L; H-SEMA-K1; CD108 antibody

Documentation

User Guide for Antibodies (1077 KB)