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CAT-1 Antibody (YA8992)

(Synonyms: CAT-1, SLC7A1, CAT1, ERR)
Customer Review

Based on 1 Customer Validation

CAT-1 Antibody (YA8992) is a Mouse-derived and non-conjugated IgG1 monoclonal antibody, targeting to CAT-1.

For research use only. We do not sell to patients.
  • Host:

    Mouse

  • Isotype:

    IgG

  • Application:

    WB, ICC/IF, IF-Tissue, IP, ELISA

  • Reactivity :

    human

  • Formulation:

    Supplied in PBS(pH7.4) containing 0.1% gelatin and < 0.1% sodium azide.

  • Conjugation:
    Non-conjugated
  • Experimental Validation Results for CAT-1 Antibody (YA8992)
  • CAT-1 Antibody (YA8992) Product Image
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To place orders, for customer services and technical support, please contact: MedChemExpress USA

Tel: 609-228-6898 E-mail: [email protected] [email protected]

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Use of MCE Products

Applications

Application
WB Info
WB: Western Blot
ICC/IF Info
ICC/IF: Immunocytochemistry/
Immunofluorescence
IF-Tissue Info
IF-Tissue: Immunofluorescence-Tissue
IP Info
IP: Immunoprecipitation
ELISA Info
ELISA: Enzyme Linked Immunosorbent Assay
Dilution Ratio 1:500-1000 1:50-500 1:50-500 1-2μg per 100-500μg Total protein 1:50-3000

Product Details

Description

CAT-1 Antibody (YA8992) is a Mouse-derived and non-conjugated IgG1 monoclonal antibody, targeting to CAT-1.

  • Host Mouse
  • Species Reactivity
    human
  • Calculated Molecular Weight Predicted band size: 71 kDa
Species Reactivity Database

OMIM: 104615

Immunogen

A synthesized peptide derived from human CAT-1.

Sensitivity

Endogenous

Purification

Affinity purified

Conjugation

Non-conjugated

Modification

Unmodified

Isotype

IgG

Product Properties

  • Appearance

    Solution

  • Formulation

    Supplied in PBS(pH7.4) containing 0.1% gelatin and < 0.1% sodium azide.

  • Concentration

    Batch-dependent, Please check the COA for the concentration of each lot. Check Lot Concentration

  • Storage & Stability

    Stored at 2-8°C for 1 year, do not freeze.

  • Shipping

    Shipping with blue ice.

Verification Images

  • Experimental Validation Results for CAT-1 Antibody (YA8992)
    Western blot analysis was performed on protein extracts (25 μg) from HT-1080 (lane 1) using CAT-1 antibody. Proteins were transferred onto a 0.45 μm PVDF membrane using the Trans-Blot® Turbo™ system for 13 min. The membrane was then blocked with 5% nonfat milk in TBST (HY-K1025) for 1 h at room temperature. The primary antibody (1:1000) and loading control antibody GAPDH Antibody (HRP) (HY-P80954A) (1:5000) were diluted in 5% nonfat milk in TBST and incubated with the membrane overnight at 4°C. After washing, the membrane of primary antibody was incubated with HRP-conjugated goat anti-rabbit/mouse IgG secondary antibody (HY-P8001/HY-P8004) (1:5000) diluted in 5% nonfat milk in TBST for 1 h at room temperature. Protein bands were visualized using an Ultra High Sensitivity ECL detection kit (HY-K1005).

Background

  • Function

    CAT-1 is a High-affinity, low capacity permease involved in the transport of the cationic amino acids (arginine, lysine and ornithine) in non-hepatic tissues

  • Subcellular Localization

    Cell membrane

  • Expression


    Tissue_Specificity: Ubiquitous.

  • Isoforms & Post-Translational Modification

    CAT-1 has an amino acid length of 629, molecular weight is 67638 Da.

  • Subunit

    Interacts with TM4SF5; the interaction is negatively regulated by arginine.

  • SwissProt ID

    P30825

  • Gene ID
    6541 [NCBI]
  • Synonyms

    CAT-1, SLC7A1, CAT1, ERR

CAT-1 Antibody (YA8992) Related Classifications

MOQ
Minimum order quantity
100 mg

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Measured by its ability to inhibit anti-CD3-induced proliferation of stimulated CTLL-2 mouse cytotoxic T cells. The ED50 this effect is 0.8023 μg/ml, corresponding to a specific activity is 1246.42 units/mg.

Assay Types
  • 1/16

    MedChemExpress Validation
     Western blot analysis was performed on protein extracts (25 μg) from HT-1080 (lane 1) using CAT-1 antibody. Proteins were transferred onto a 0.45 μm PVDF membrane using the Trans-Blot® Turbo™ system for 13 min. The membrane was then blocked with 5% nonfat milk in TBST (HY-K1025) for 1 h at room temperature. The primary antibody (1:1000) and loading control antibody GAPDH Antibody (HRP) (HY-P80954A) (1:5000) were diluted in 5% nonfat milk in TBST and incubated with the membrane overnight at 4°C. After washing, the membrane of primary antibody was incubated with HRP-conjugated goat anti-rabbit/mouse IgG secondary antibody (HY-P8001/HY-P8004) (1:5000) diluted in 5% nonfat milk in TBST for 1 h at room temperature. Protein bands were visualized using an Ultra High Sensitivity ECL detection kit (HY-K1005).

  • CAT-1 Antibody (YA8992) Product Image
    1/16
  • Experimental Validation Results for CAT-1 Antibody (YA8992)
  • CAT-1 Antibody (YA8992) Product Image
  • Experimental Validation Results for CAT-1 Antibody (YA8992)
    1/1
    MedChemExpress Validation
     Western blot analysis was performed on protein extracts (25 μg) from HT-1080 (lane 1) using CAT-1 antibody. Proteins were transferred onto a 0.45 μm PVDF membrane using the Trans-Blot® Turbo™ system for 13 min. The membrane was then blocked with 5% nonfat milk in TBST (HY-K1025) for 1 h at room temperature. The primary antibody (1:1000) and loading control antibody GAPDH Antibody (HRP) (HY-P80954A) (1:5000) were diluted in 5% nonfat milk in TBST and incubated with the membrane overnight at 4°C. After washing, the membrane of primary antibody was incubated with HRP-conjugated goat anti-rabbit/mouse IgG secondary antibody (HY-P8001/HY-P8004) (1:5000) diluted in 5% nonfat milk in TBST for 1 h at room temperature. Protein bands were visualized using an Ultra High Sensitivity ECL detection kit (HY-K1005).
  • Experimental Validation Results for CAT-1 Antibody (YA8992)
  • CAT-1 Antibody (YA8992) Product Image
    MedChemExpress Validation

    Western blot analysis was performed on protein extracts (25 μg) from HT-1080 (lane 1) using CAT-1 antibody. Proteins were transferred onto a 0.45 μm PVDF membrane using the Trans-Blot® Turbo™ system for 13 min. The membrane was then blocked with 5% nonfat milk in TBST (HY-K1025) for 1 h at room temperature. The primary antibody (1:1000) and loading control antibody GAPDH Antibody (HRP) (HY-P80954A) (1:5000) were diluted in 5% nonfat milk in TBST and incubated with the membrane overnight at 4°C. After washing, the membrane of primary antibody was incubated with HRP-conjugated goat anti-rabbit/mouse IgG secondary antibody (HY-P8001/HY-P8004) (1:5000) diluted in 5% nonfat milk in TBST for 1 h at room temperature. Protein bands were visualized using an Ultra High Sensitivity ECL detection kit (HY-K1005).

  • CAT-1 Antibody (YA8992) Product Image WB