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YTHDF2 Antibody (YA6146)

(Synonyms: HGRG8, YTHDF2, YTH domain-containing family protein 2, DF2, CLL-associated antigen KW-14, High-glucose-regulated protein 8, Renal carcinoma antigen NY-REN-2)
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Based on 1 Customer Validation

YTHDF2 Antibody (YA6146) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to YTHDF2.

For research use only. We do not sell to patients.
  • Host:

    Rabbit

  • Isotype:

    IgG

  • Application:

    WB, IHC-P, ICC/IF, IP, ELISA

  • Reactivity :

    Human, Mouse, Rat

  • Formulation:

    Supplied in PBS, 50% glycerol, 0.05% Proclin 300, 0.05%BSA

  • Conjugation:
    Non-conjugated
  • Experimental Validation Results for YTHDF2 Antibody (YA6146)
  • YTHDF2 Antibody (YA6146) Product Image
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To place orders, for customer services and technical support, please contact: MedChemExpress USA

Tel: 609-228-6898 E-mail: [email protected] [email protected]

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Use of MCE Products

Applications

Application
IHC-P Info
IHC-P: Immunohistochemistry-Paraffin
WB Info
WB: Western Blot
ICC/IF Info
ICC/IF: Immunocytochemistry/
Immunofluorescence
ELISA Info
ELISA: Enzyme Linked Immunosorbent Assay
IP Info
IP: Immunoprecipitation
Dilution Ratio 1:200-1:1000 1:1000-1:5000 1:200-1:1000 1:5000-1:20000 1:50-1:200

Product Details

Description

YTHDF2 Antibody (YA6146) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to YTHDF2.

  • Host Rabbit
  • Clonality Monoclonal
  • Species Reactivity
    Human, Mouse, Rat
  • Observed Molecular Weight
    Observed band size: 62 kDa Info
    Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.
  • Calculated Molecular Weight Predicted band size: 62 kDa
Purification

Protein A

Conjugation

Non-conjugated

Modification

Unmodified

Isotype

IgG

Product Properties

  • Appearance

    Solution

  • Formulation

    Supplied in PBS, 50% glycerol, 0.05% Proclin 300, 0.05%BSA

  • Concentration

    Batch-dependent, Please check the COA for the concentration of each lot. Check Lot Concentration

  • Storage & Stability

    Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

  • Shipping

    Shipping with blue ice.

Verification Images

  • Experimental Validation Results for YTHDF2 Antibody (YA6146)
    Western blot analysis was performed on protein extracts (30 μg) from HT-1080 (lane 2), SH-SY5Y (lane 3), HeLa (lane 4), HepG2 (lane 5), and A549 (lane 6) using YTHDF2 antibody. Proteins were transferred onto a 0.45 μm PVDF membrane using the Trans-Blot® Turbo™ system for 13 min. The membrane was then blocked with 5% nonfat milk in TBST (HY-K1025) for 1 h at room temperature. Thhe primary antibody (1:1000) and loading control antibody GAPDH Antibody (HRP) (HY-P80954A) (1:2500) were diluted in 5% nonfat milk in TBST and incubated with the membrane overnight at 4°C. After washing, the membrane of primary antibody was incubated with HRP-conjugated goat anti-rabbit IgG (H&L) secondary antibody (HY-P8001) (1:5000) diluted in 5% nonfat milk in TBST for 1 h at room temperature. Protein bands were visualized using an Ultra High Sensitivity ECL detection kit (HY-K1005).

Background

  • Function

    YTHDF2 is a cytoplasmic m6A reader that controls m6A-modified RNA fate through mRNA degradation and, in selected contexts, translation regulation[1][2]. Mechanistically, YTHDF2-mediated mRNA decay targets multiple TGF-β signaling components in early postnatal mouse hippocampal neural stem cells, thereby limiting quiescence acquisition and supporting neurogenesis[1]. In glioma models, YTHDF2 accelerates UBXN1 mRNA degradation through METTL3-mediated m6A recognition, activates NF-κB signaling, and promotes malignant progression[3]. In glioblastoma stem cells, YTHDF2 instead stabilizes MYC and VEGFA transcripts, identifies a cancer-specific dependency, and links the YTHDF2-MYC-IGFBP3 axis to tumor growth[2]. Compared with related YTHDF isoforms, YTHDF proteins share m6A-reader activity but display distinct cellular functions because their low-complexity N termini, post-translational modifications, subcellular localization, and partner competition differ[4]. For experimental applications, linsitinib preferentially targeted YTHDF2-expressing glioblastoma stem cells, while a YTHDF2-specific small-molecule inhibitor suppressed tumor-cell translation and reversed paclitaxel resistance in ovarian cancer models[2][5].

  • Subcellular Localization

    Cytoplasm, cytosol; Cytoplasm, P-body; Cytoplasm, Stress granule; Nucleus

  • Expression


    Tissue_specificity:Highly expressed in induced pluripotent stem cells (iPSCs) and down-regulated during neural differentiation

    Induction:Following heat shock stress

  • Isoforms & Post-Translational Modification

    Q9Y5A9 has 2 isomers: Q9Y5A9-1: 62334 Da (predicted); Q9Y5A9-2: 56877 Da (predicted).
    Ubiquitinated by the SCF(SKP2) complex, leading to its degradation

  • Subunit

    Interacts with CNOT1; interaction is direct and promotes recruitment of the CCR4-NOT complex (PubMed:27558897, PubMed:32492408). Interacts with YTHDF3 (PubMed:28106072). Interacts with RIDA/HRSP12; interaction leads to recruitment of the ribonuclease P/MRP complex (PubMed:30930054)

  • SwissProt ID

    Q9Y5A9

  • Gene ID
    51441 [NCBI]
  • Synonyms

    HGRG8, YTHDF2, YTH domain-containing family protein 2, DF2, CLL-associated antigen KW-14, High-glucose-regulated protein 8, Renal carcinoma antigen NY-REN-2

[1]. Zhang F, et al. m6A/YTHDF2-mediated mRNA decay targets TGF-β signaling to suppress the quiescence acquisition of early postnatal mouse hippocampal NSCs. Cell Stem Cell. 2025 Jan 2;32(1):144-156.e8. [Content Brief]

[2]. Dixit D, et al. The RNA m6A Reader YTHDF2 Maintains Oncogene Expression and Is a Targetable Dependency in Glioblastoma Stem Cells. Cancer Discov. 2021 Feb;11(2):480-499. [Content Brief]

[3]. Chai RC, et al. YTHDF2 facilitates UBXN1 mRNA decay by recognizing METTL3-mediated m6A modification to activate NF-κB and promote the malignant progression of glioma. J Hematol Oncol. 2021 Jul 10;14(1):109. [Content Brief]

[4]. Zou Z, et al. The YTHDF proteins display distinct cellular functions on m6A-modified RNA. Trends Biochem Sci. 2024 Jul;49(7):611-621. [Content Brief]

[5]. Liu T, et al. The RNA-stability-independent role of the RNA m6A reader YTHDF2 in promoting protein translation to confer tumor chemotherapy resistance. Mol Cell. 2025 Jun 19;85(12):2320-2336.e9. [Content Brief]

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YTHDF2 Antibody (YA6146) Related Classifications

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100 mg

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Measured by its ability to inhibit anti-CD3-induced proliferation of stimulated CTLL-2 mouse cytotoxic T cells. The ED50 this effect is 0.8023 μg/ml, corresponding to a specific activity is 1246.42 units/mg.

Assay Types
  • 1/16

    MedChemExpress Validation
     Western blot analysis was performed on protein extracts (30 μg) from HT-1080 (lane 2), SH-SY5Y (lane 3), HeLa (lane 4), HepG2 (lane 5), and A549 (lane 6) using YTHDF2 antibody. Proteins were transferred onto a 0.45 μm PVDF membrane using the Trans-Blot® Turbo™ system for 13 min. The membrane was then blocked with 5% nonfat milk in TBST (HY-K1025) for 1 h at room temperature. Thhe primary antibody (1:1000) and loading control antibody GAPDH Antibody (HRP) (HY-P80954A) (1:2500) were diluted in 5% nonfat milk in TBST and incubated with the membrane overnight at 4°C. After washing, the membrane of primary antibody was incubated with HRP-conjugated goat anti-rabbit IgG (H&L) secondary antibody (HY-P8001) (1:5000) diluted in 5% nonfat milk in TBST for 1 h at room temperature. Protein bands were visualized using an Ultra High Sensitivity ECL detection kit (HY-K1005).

  • YTHDF2 Antibody (YA6146) Product Image
    1/16
  • Experimental Validation Results for YTHDF2 Antibody (YA6146)
  • YTHDF2 Antibody (YA6146) Product Image
  • Experimental Validation Results for YTHDF2 Antibody (YA6146)
    1/1
    MedChemExpress Validation
     Western blot analysis was performed on protein extracts (30 μg) from HT-1080 (lane 2), SH-SY5Y (lane 3), HeLa (lane 4), HepG2 (lane 5), and A549 (lane 6) using YTHDF2 antibody. Proteins were transferred onto a 0.45 μm PVDF membrane using the Trans-Blot® Turbo™ system for 13 min. The membrane was then blocked with 5% nonfat milk in TBST (HY-K1025) for 1 h at room temperature. Thhe primary antibody (1:1000) and loading control antibody GAPDH Antibody (HRP) (HY-P80954A) (1:2500) were diluted in 5% nonfat milk in TBST and incubated with the membrane overnight at 4°C. After washing, the membrane of primary antibody was incubated with HRP-conjugated goat anti-rabbit IgG (H&L) secondary antibody (HY-P8001) (1:5000) diluted in 5% nonfat milk in TBST for 1 h at room temperature. Protein bands were visualized using an Ultra High Sensitivity ECL detection kit (HY-K1005).
  • Experimental Validation Results for YTHDF2 Antibody (YA6146)
  • YTHDF2 Antibody (YA6146) Product Image
    MedChemExpress Validation

    Western blot analysis was performed on protein extracts (30 μg) from HT-1080 (lane 2), SH-SY5Y (lane 3), HeLa (lane 4), HepG2 (lane 5), and A549 (lane 6) using YTHDF2 antibody. Proteins were transferred onto a 0.45 μm PVDF membrane using the Trans-Blot® Turbo™ system for 13 min. The membrane was then blocked with 5% nonfat milk in TBST (HY-K1025) for 1 h at room temperature. Thhe primary antibody (1:1000) and loading control antibody GAPDH Antibody (HRP) (HY-P80954A) (1:2500) were diluted in 5% nonfat milk in TBST and incubated with the membrane overnight at 4°C. After washing, the membrane of primary antibody was incubated with HRP-conjugated goat anti-rabbit IgG (H&L) secondary antibody (HY-P8001) (1:5000) diluted in 5% nonfat milk in TBST for 1 h at room temperature. Protein bands were visualized using an Ultra High Sensitivity ECL detection kit (HY-K1005).

  • YTHDF2 Antibody (YA6146) Product Image WB