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HBEGF Antibody (YA1801)

(Synonyms: DTR; DTS; DTSF; HB-EGF; HBEGF; HEGFL)
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Based on 1 Customer Validation

HBEGF Antibody (YA1801) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to HBEGF.

For research use only. We do not sell to patients.
  • Host:

    Rabbit

  • Isotype:

    IgG

  • Application:

    WB

  • Reactivity :

    Human, Rat

  • Formulation:

    Supplied in 50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40%Glycerol, 0.01% sodium azide and 0.05% BSA.

  • Conjugation:
    Non-conjugated
  • Experimental Validation Results for HBEGF Antibody (YA1801)
  • HBEGF Antibody (YA1801) Product Image
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To place orders, for customer services and technical support, please contact: MedChemExpress USA

Tel: 609-228-6898 E-mail: [email protected] [email protected]

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Use of MCE Products

Applications

Application
WB Info
WB: Western Blot
Dilution Ratio 1:500-1:1000

Product Details

Description

HBEGF Antibody (YA1801) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to HBEGF.

  • Host Rabbit
  • Clonality Recombinant,Monoclonal
  • Species Reactivity
    Human, Rat
  • Observed Molecular Weight
    Observed band size: 23 kDa Info
    Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.
  • Calculated Molecular Weight Predicted band size: 23 kDa
Species Reactivity Database
Immunogen

A synthesized peptide derived from human HB EGF aa50-110/208.

Sensitivity

Endogenous

Purification

Affinity Chromatography

Conjugation

Non-conjugated

Modification

Unmodified

Isotype

IgG

RRID

AB_3104176

Product Properties

  • Appearance

    Solution

  • Formulation

    Supplied in 50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40%Glycerol, 0.01% sodium azide and 0.05% BSA.

  • Concentration

    Batch-dependent, Please check the COA for the concentration of each lot. Check Lot Concentration

  • Storage & Stability

    Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

  • Shipping

    Shipping with blue ice.

Verification Images

  • Experimental Validation Results for HBEGF Antibody (YA1801)
    Western blot analysis of extracts from K562 (lane 2(20μg) and K562 (lane 3(40μg) using HBEGF (HY-P82056) Rabbit mAb. Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P80438, 1/10000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Mouse IgG-HRP Secondary Antibody (1/10000) was used for 1 hour at room temperature.

Background

  • Function

    Heparin-binding epidermal growth factor-like growth factor (HB-EGF) is a heparin-binding member of the EGF family. It is a potent mitogen and chemotactic factor for fibroblasts and smooth muscle cells. HB-EGF is synthesized as a membrane-anchored form (pr

  • Subcellular Localization

    Secreted, extracellular space; Cell membrane; Single-pass type I membrane protein

  • Subunit

    Interacts with FBLN1 (By similarity). Interacts with EGFR and ERBB4

  • SwissProt ID

    Q99075

  • Gene ID
    1839 [NCBI]
  • Synonyms

    DTR; DTS; DTSF; HB-EGF; HBEGF; HEGFL

  • Research Field

    Signal Transduction

HBEGF Antibody (YA1801) Related Classifications

MOQ
Minimum order quantity
100 mg

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Measured by its ability to inhibit anti-CD3-induced proliferation of stimulated CTLL-2 mouse cytotoxic T cells. The ED50 this effect is 0.8023 μg/ml, corresponding to a specific activity is 1246.42 units/mg.

Assay Types
  • 1/16

    MedchemExpress Validation
     Western blot analysis of extracts from K562 (lane 2(20μg) and K562 (lane 3(40μg) using HBEGF (HY-P82056) Rabbit mAb. Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P80438, 1/10000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Mouse IgG-HRP Secondary Antibody (1/10000) was used for 1 hour at room temperature.

  • HBEGF Antibody (YA1801) Product Image
    1/16
  • Experimental Validation Results for HBEGF Antibody (YA1801)
  • HBEGF Antibody (YA1801) Product Image
  • Experimental Validation Results for HBEGF Antibody (YA1801)
    1/1
    MedchemExpress Validation
     Western blot analysis of extracts from K562 (lane 2(20μg) and K562 (lane 3(40μg) using HBEGF (HY-P82056) Rabbit mAb. Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P80438, 1/10000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Mouse IgG-HRP Secondary Antibody (1/10000) was used for 1 hour at room temperature.
  • Experimental Validation Results for HBEGF Antibody (YA1801)
  • HBEGF Antibody (YA1801) Product Image
    MedchemExpress Validation

    Western blot analysis of extracts from K562 (lane 2(20μg) and K562 (lane 3(40μg) using HBEGF (HY-P82056) Rabbit mAb. Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P80438, 1/10000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Mouse IgG-HRP Secondary Antibody (1/10000) was used for 1 hour at room temperature.

  • HBEGF Antibody (YA1801) Product Image WB