PIN4 Antibody (YA1641)(PBS only)

Cat. No.: HY-P81896A: Data Sheet User Guide for Antibodies
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PIN4 Antibody (YA1641) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to PIN4.

For research use only. We do not sell to patients.

Size		Stock
50 μL		Get quote
100 μL		Get quote

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WB: Western Blot;
IHC-P: Immunohistochemistry-Paraffin;
IHC-F: Immunohistochemistry-Frozen;
ICC/IF: Immunocytochemistry/Immunofluorescence;
IF-Tissue: Immunofluorescence-Tissue;
mIHC: Multiplex Immunohistochemical;
IP: Immunoprecipitation;
ChIP: Chromatin Immunoprecipitation;
FC: Flow Cytometry;
ELISA: Enzyme Linked Immunosorbent Assay

Product Detail
Background
Documentation

Description

PIN4 Antibody (YA1641) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to PIN4.

Host

Rabbit

Molecular Weight

Predicted band size: 14 kDa;

Observed band size: 14 kDa

Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.

Species Reactivity

Human, Rat, Hamster

SwissProt ID

Q9Y237

Gene ID

5303 [NCBI]

Immunogen

A synthetic peptide of human PIN4

Application &
Dilution Ratio

Application	Dilution Ratio
WB WB: Western Blot	1:500-1:1000
IP IP: Immunoprecipitation	1:20

Purity	Affinity Purified	Conjugation	Non-conjugated
Isotype	IgG

Appearance

Liquid

Formulation

Supplied in PBS, pH 7.4.

Storage & Stability

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

Shipping

Shipping with blue ice.

Background

Function：Isoform 1 is involved as a ribosomal RNA processing factor in ribosome biogenesis. Binds to tightly bent AT-rich stretches of double-stranded DNA; Isoform 2 binds to double-stranded DNA

Subcellular Localization：Nucleus, nucleolus; Cytoplasm, cytoskeleton, spindle; Cytoplasm; Mitochondrion; Mitochondrion matrix

Expression：
Tissue_specificity:Isoform 2 is much more stable than isoform 1 (at protein level) . Ubiquitous. Isoform 1 and isoform 2 are expressed in kidney, liver, blood vessel, brain, mammary gland, skeletal muscle, small intestine and submandibularis. Isoform 1 transcripts are much more abundant than isoform 2 in each tissue analyzed

Isoforms & Post-Translational Modification：Q9Y237 has 3 isomers: Q9Y237-1: 13810 Da (predicted); Q9Y237-2: 16608 Da (predicted); Q9Y237-3: 14179 Da (predicted).
Phosphorylated. Isoform 1 phosphorylation occurs both in the nucleus and the cytoplasm. Isoform 1 phosphorylation at Ser-19 does not affect its PPIase activity but is required for nuclear localization, and the dephosphorylation is a prerequisite for the binding to DNA. The unphosphorylated isoform 1 associates with the pre-rRNP complexes in the nucleus;Isoform 2 is sumoylated with SUMO2 and SUMO3

Subunit：Isoform 1 is found in pre-ribosomal ribonucleoprotein (pre-rRNP) complexes

Research Field

Epigenetics and Nuclear Signaling

Synonyms

EPVH; PAR14; PAR17

Documentation

User Guide for Antibodies (1077 KB)