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Nicastrin Antibody (YA1397)(PBS only)

(Synonyms: APH2; Ncstn; NCT; Nicastrin)

Nicastrin Antibody (YA1397) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to Nicastrin.

For research use only. We do not sell to patients.
  • Host:

    Rabbit

  • Isotype:

    IgG

  • Application:

    WB, IP

  • Reactivity :

    Human, Mouse, Rat

  • Formulation:

    Supplied in PBS, pH 7.4.

  • Conjugation:
    Non-conjugated
  • Experimental Validation Results for Nicastrin Antibody (YA1397)(PBS only)
  • Nicastrin Antibody (YA1397)(PBS only) Product Image
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To place orders, for customer services and technical support, please contact: MedChemExpress USA

Tel: 609-228-6898 E-mail: [email protected] [email protected]

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Applications

Application
WB Info
WB: Western Blot
IP Info
IP: Immunoprecipitation
Dilution Ratio 1:500-1:1000 1:20

Product Details

Description

Nicastrin Antibody (YA1397) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to Nicastrin.

  • Host Rabbit
  • Species Reactivity
    Human, Mouse, Rat
  • Observed Molecular Weight
    Observed band size: 110-120 kDa Info
    Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.
  • Calculated Molecular Weight Predicted band size: 78 kDa
Immunogen

A synthetic peptide of human Nicastrin

Purification

Affinity Purified

Conjugation

Non-conjugated

Isotype

IgG

Product Properties

  • Appearance

    Liquid

  • Formulation

    Supplied in PBS, pH 7.4.

  • Storage & Stability

    Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

  • Shipping

    Shipping with blue ice.

Verification Images

  • Experimental Validation Results for Nicastrin Antibody (YA1397)(PBS only)
    Western blot analysis was performed on extracts from Hela (lane 1, 15 μg), K562 (lane 2, 15 μg), U-87MG (lane 3, 15 μg), and C6 (lane 4, 15 μg) using Nicastrin Rabbit mAb.Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST at 4°C overnight.The primary antibody (1:1000 dilution) and the loading control antibody (beta-Tubulin, HY-P80487, 1:40000 dilution) were incubated in 5% non-fat milk in TBST for 1 hour at 37°C.Goat Anti-Rabbit IgG-HRP Secondary Antibody (1:20000 dilution) was then applied for 40 minutes at 37°C.

Background

  • Function

    Nicastrin is essential subunit of the gamma-secretase complex, an endoprotease complex that catalyzes the intramembrane cleavage of integral membrane proteins such as Notch receptors and APP (amyloid-beta precursor protein). The gamma-secretase complex plays a role in Notch and Wnt signaling cascades and regulation of downstream processes via its role in processing key regulatory proteins, and by regulating cytosolic CTNNB1 levels[1][2][3][4][5][6].

  • Subcellular Localization

    Membrane; Single-pass type I membrane protein; Cytoplasmic vesicle membrane; Single-pass type I membrane protein; Melanosome

  • Expression


    Tissue_specificity:Detected in brain (at protein level) (PubMed:10993067) . Widely expressed (PubMed:11396676)

    Induction:Constitutively expressed in neural cells

  • Isoforms & Post-Translational Modification

    Q92542 has 2 isomers: Q92542-1: 78411 Da (predicted); Q92542-2: 76744 Da (predicted).
    N-glycosylated

  • Subunit

    Component of the gamma-secretase complex (PubMed:10993067, PubMed:30598546, PubMed:30630874). The functional gamma-secretase complex is composed of at least four polypeptides: a presenilin homodimer (PSEN1 or PSEN2), nicastrin (NCSTN), APH1 (APH1A or APH1B) and PSENEN/PEN2 (PubMed:12740439, PubMed:25043039, PubMed:25918421, PubMed:26280335, PubMed:26623517, PubMed:30598546, PubMed:30630874). Binds to proteolytic processed C-terminal fragments C83 and C99 of the amyloid precursor protein (APP) (PubMed:10993067, PubMed:30630874). Interacts with PSEN1 and PSEN2 (PubMed:10993067)

  • SwissProt ID

    Q92542

  • Gene ID
    23385 [NCBI]
  • Synonyms

    APH2; Ncstn; NCT; Nicastrin

  • Research Field

    Neuroscience

Nicastrin Antibody (YA1397)(PBS only) Related Classifications

MOQ
Minimum order quantity
100 mg

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Measured by its ability to inhibit anti-CD3-induced proliferation of stimulated CTLL-2 mouse cytotoxic T cells. The ED50 this effect is 0.8023 μg/ml, corresponding to a specific activity is 1246.42 units/mg.

Assay Types
  • 1/16

    MedchemExpress Validation
     Western blot analysis was performed on extracts from Hela (lane 1, 15 μg), K562 (lane 2, 15 μg), U-87MG (lane 3, 15 μg), and C6 (lane 4, 15 μg) using Nicastrin Rabbit mAb.Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST at 4°C overnight.The primary antibody (1:1000 dilution) and the loading control antibody (beta-Tubulin, HY-P80487, 1:40000 dilution) were incubated in 5% non-fat milk in TBST for 1 hour at 37°C.Goat Anti-Rabbit IgG-HRP Secondary Antibody (1:20000 dilution) was then applied for 40 minutes at 37°C.

  • Nicastrin Antibody (YA1397)(PBS only) Product Image
    1/16
  • Experimental Validation Results for Nicastrin Antibody (YA1397)(PBS only)
  • Nicastrin Antibody (YA1397)(PBS only) Product Image
  • Experimental Validation Results for Nicastrin Antibody (YA1397)(PBS only)
    1/1
    MedchemExpress Validation
     Western blot analysis was performed on extracts from Hela (lane 1, 15 μg), K562 (lane 2, 15 μg), U-87MG (lane 3, 15 μg), and C6 (lane 4, 15 μg) using Nicastrin Rabbit mAb.Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST at 4°C overnight.The primary antibody (1:1000 dilution) and the loading control antibody (beta-Tubulin, HY-P80487, 1:40000 dilution) were incubated in 5% non-fat milk in TBST for 1 hour at 37°C.Goat Anti-Rabbit IgG-HRP Secondary Antibody (1:20000 dilution) was then applied for 40 minutes at 37°C.
  • Experimental Validation Results for Nicastrin Antibody (YA1397)(PBS only)
  • Nicastrin Antibody (YA1397)(PBS only) Product Image
    MedchemExpress Validation

    Western blot analysis was performed on extracts from Hela (lane 1, 15 μg), K562 (lane 2, 15 μg), U-87MG (lane 3, 15 μg), and C6 (lane 4, 15 μg) using Nicastrin Rabbit mAb.Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST at 4°C overnight.The primary antibody (1:1000 dilution) and the loading control antibody (beta-Tubulin, HY-P80487, 1:40000 dilution) were incubated in 5% non-fat milk in TBST for 1 hour at 37°C.Goat Anti-Rabbit IgG-HRP Secondary Antibody (1:20000 dilution) was then applied for 40 minutes at 37°C.

  • Nicastrin Antibody (YA1397)(PBS only) Product Image WB