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  4. Monoclonal Antibodies Recombinant Antibodies Modification Specific Antibodies
  5. Phospho-eIF4G1 (Ser1108) Antibody (YA201)

Phospho-eIF4G1 (Ser1108) Antibody (YA201)

Cat. No.: HY-P80459
COA
SDS
User Guide for Antibodies Technical Support

Phospho-eIF4G1 (Ser1108) Antibody (YA201) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to Phospho-eIF4G1 (Ser1108).

For research use only. We do not sell to patients.

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Top Publications Citing Use of Products
  • WB: Western Blot;
  • IHC-P: Immunohistochemistry-Paraffin;
  • IHC-F: Immunohistochemistry-Frozen;
  • ICC/IF: Immunocytochemistry/Immunofluorescence;
  • IF-Tissue: Immunofluorescence-Tissue;
  • mIHC: Multiplex Immunohistochemical;
  • IP: Immunoprecipitation;
  • ChIP: Chromatin Immunoprecipitation;
  • FC: Flow Cytometry;
  • ELISA: Enzyme Linked Immunosorbent Assay

  • Product Detail

  • Background

  • Documentation

Description

Phospho-eIF4G1 (Ser1108) Antibody (YA201) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to Phospho-eIF4G1 (Ser1108).
Host

Rabbit
Clonality

Recombinant, Monoclonal
Molecular Weight
Predicted band size: 175 kDa;
Observed band size: 220 kDa
Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.
Species Reactivity
Human
SwissProt ID
Gene ID
Immunogen

Synthetic phosphopeptide corresponding to residues surrounding Ser1108 of Human eIF4G1.The exact sequence is proprietary to MCE.
Application &
Dilution Ratio
Application Dilution Ratio
WB
WB: Western Blot
1:1000
ICC/IF
ICC/IF: Immunocytochemistry/Immunofluorescence
1:50
Sensitivity Endogenous Purity Protein A affinity purified.
Conjugation Non-conjugated Modification Phosphorylated
Isotype IgG  
Appearance

Liquid
Formulation

Supplied in 1*PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide
Storage & Stability

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.
Shipping

Shipping with blue ice.
Background
Function：Component of the protein complex eIF4F, which is involved in the recognition of the mRNA cap, ATP-dependent unwinding of 5'-terminal secondary structure and recruitment of mRNA to the ribosome (PubMed:29987188). Exists in two complexes, either with EIF1 or with EIF4E (mutually exclusive) (PubMed:29987188). Together with EIF1, is required for leaky scanning, in particular for avoiding cap-proximal start codon (PubMed:29987188). Together with EIF4E, antagonizes the scanning promoted by EIF1-EIF4G1 and locates the start codon (through a TISU element) without scanning (PubMed:29987188). As a member of the eIF4F complex, required for endoplasmic reticulum stress-induced ATF4 mRNA translation (PubMed:29062139)
Subcellular Localization：Cytoplasm; Nucleus; Cytoplasm, Stress granule
Isoforms & Post-Translational Modification：Q04637 has 8 isomers: Q04637-1: 175491 Da (predicted); Q04637-3: 171514 Da (predicted); Q04637-4: 166619 Da (predicted); Q04637-5: 158517 Da (predicted); Q04637-6: 154805 Da (predicted); Q04637-7: 154933 Da (predicted); Q04637-8: 175619 Da (predicted); Q04637-9: 176237 Da (predicted).
Phosphorylated at multiple sites in vivo. Phosphorylation at Ser-1185 by PRKCA induces binding to MKNK1;Following infection by certain enteroviruses, rhinoviruses and aphthoviruses, EIF4G1 is cleaved by the viral protease 2A, or the leader protease in the case of aphthoviruses. This shuts down the capped cellular mRNA transcription
Subunit：eIF4F is a multi-subunit complex, the composition of which varies with external and internal environmental conditions. It is composed of at least EIF4A, EIF4E (cap-binding) and EIF4G1/EIF4G3 (PubMed:7651417, PubMed:7935836, PubMed:9372926). Interacts with eIF3 complex, mutually exclusive with EIF4A1 or EIF4A2, EIF4E and through its N-terminus with PABPC1 (PubMed:10970864, PubMed:10996799, PubMed:12086624, PubMed:16698552, PubMed:7651417, PubMed:7935836, PubMed:9372926, PubMed:9857202). Interacts with EIF4E or with EIF1 (mutually exclusive) through a common binding site (PubMed:29987188). Interacts through its C-terminus with the serine/threonine kinases MKNK1, and with MKNK2 (PubMed:11154262, PubMed:9878069). Appears to act as a scaffold protein, holding these enzymes in place to phosphorylate EIF4E (PubMed:11154262, PubMed:9878069). Non-phosphorylated EIF4EBP1 competes with EIF4G1/EIF4G3 to interact with EIF4E; insulin stimulated MAP-kinase (MAPK1 and MAPK3) phosphorylation of EIF4EBP1 causes dissociation of the complex allowing EIF4G1/EIF4G3 to bind and consequent initiation of translation (PubMed:8521827). EIF4G1/EIF4G3 interacts with PABPC1 to bring about circularization of the mRNA (PubMed:10970864, PubMed:10996799, PubMed:12086624, PubMed:9857202). Interacts with EIF4E3 (By similarity). Interacts with CIRBP and MIF4GD (PubMed:10970864, PubMed:10996799, PubMed:16513844, PubMed:18025107). Interacts with RBM4 (PubMed:17284590). Interacts with HNRNPD/AUF1; the interaction requires RNA (PubMed:24423872). Interacts with DDX3X; the interaction requires RNA (PubMed:22872150). Interacts with DAZAP2 (PubMed:17984221)
RRID
Database

Entrez Gene: 1981 Human

SwissProt: Q04637 Human

OMIM: 614251 Human

Research Field

Epigenetics and Nuclear Signaling
Documentation
SDS (251 KB)

COA (206 KB)

User Guide for Antibodies (1077 KB)

Phospho-eIF4G1 (Ser1108) Antibody (YA201) Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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