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Peroxiredoxin 5 Antibody (YA1591)(PBS only)

(Synonyms: ACR1; AOEB166; B166; Peroxiredoxin V; Peroxiredoxin-5; PLP; PMP20; PRDX5; PRDX6; Prx-V; PRXV; SBBI10; Thioredoxin reductase)

Peroxiredoxin 5 Antibody (YA1591) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to Peroxiredoxin 5.

For research use only. We do not sell to patients.
  • Host:

    Rabbit

  • Isotype:

    IgG

  • Application:

    WB, IHC-P, IP

  • Reactivity :

    Human, Mouse, Rat

  • Formulation:

    Supplied in PBS, pH 7.4.

  • Conjugation:
    Non-conjugated
ALL WB IHC-P
  • Experimental Validation Results for Peroxiredoxin 5 Antibody (YA1591)(PBS only)
  • Experimental Validation Results for Peroxiredoxin 5 Antibody (YA1591)(PBS only)
  • Experimental Validation Results for Peroxiredoxin 5 Antibody (YA1591)(PBS only)
  • Peroxiredoxin 5 Antibody (YA1591)(PBS only) Product Image
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To place orders, for customer services and technical support, please contact: MedChemExpress USA

Tel: 609-228-6898 E-mail: [email protected] [email protected]

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Use of MCE Products

Applications

Application
WB Info
WB: Western Blot
IHC-P Info
IHC-P: Immunohistochemistry-Paraffin
IP Info
IP: Immunoprecipitation
Dilution Ratio 1:500-1:1000 1:50-1:100 1:20

Product Details

Description

Peroxiredoxin 5 Antibody (YA1591) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to Peroxiredoxin 5.

  • Host Rabbit
  • Species Reactivity
    Human, Mouse, Rat
  • Observed Molecular Weight
    Observed band size: 17 kDa Info
    Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.
  • Calculated Molecular Weight Predicted band size: 22 kDa
Immunogen

A synthetic peptide of human Peroxiredoxin 5

Purification

Affinity Purified

Conjugation

Non-conjugated

Isotype

IgG

Product Properties

  • Appearance

    Liquid

  • Formulation

    Supplied in PBS, pH 7.4.

  • Storage & Stability

    Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

  • Shipping

    Shipping with blue ice.

Verification Images

  • Experimental Validation Results for Peroxiredoxin 5 Antibody (YA1591)(PBS only)
    Western blot analysis of extracts from HepG2 (lane 2(20μg) and HepG2 (lane 3 (40μg) using Peroxiredoxin 5 Rabbit mAb. Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P80438, 1/10000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Mouse IgG-HRP Secondary Antibody (1/10000) was used for 1 hour at room temperature.
  • Experimental Validation Results for Peroxiredoxin 5 Antibody (YA1591)(PBS only)
    Immunohistochemical analysis of paraffin-embedded Mouse kidney tissue using Peroxiredoxin 5 Antibody (YA1591). The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked in QuickBlock for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HY-P81846, 1/100) in 4℃ overnight. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
  • Experimental Validation Results for Peroxiredoxin 5 Antibody (YA1591)(PBS only)
    Immunohistochemical analysis of paraffin-embedded Mouse kidney tissue using Peroxiredoxin 5 Antibody (YA1591). The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked in QuickBlock for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HY-P81846, 1/100) in 4℃ overnight. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Background

  • Function

    Peroxiredoxin 5 is a Thiol-specific peroxidase that catalyzes the reduction of hydrogen peroxide and organic hydroperoxides to water and alcohols, respectively. Plays a role in cell protection against oxidative stress by detoxifying peroxides and as sensor of hydrogen peroxide-mediated signaling events

  • Subcellular Localization

    Mitochondrion; Cytoplasm; Peroxisome matrix

  • Expression


    Tissue_specificity:Widely expressed

  • Isoforms & Post-Translational Modification

    P30044 has 4 isomers: P30044-1: 22086 Da (predicted); P30044-2: 17031 Da (predicted); P30044-3: 17454 Da (predicted); P30044-4: 12897 Da (predicted).
    S-palmitoylated (PubMed:31740833). Palmitoylation occurs on the active site, inhibiting its reactivity; therefore PRDX5 palmitoylation status determines its antioxidant capacity (PubMed:31740833);S-palmitoylated (PubMed:31740833). Depalmitoylated by ABHD10 (PubMed:31740833)

  • Subunit

    Monomer

  • SwissProt ID

    P30044

  • Gene ID
    25824 [NCBI]
  • Synonyms

    ACR1; AOEB166; B166; Peroxiredoxin V; Peroxiredoxin-5; PLP; PMP20; PRDX5; PRDX6; Prx-V; PRXV; SBBI10; Thioredoxin reductase

  • Research Field

    Cell Biology

Peroxiredoxin 5 Antibody (YA1591)(PBS only) Related Classifications

MOQ
Minimum order quantity
100 mg

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Measured by its ability to inhibit anti-CD3-induced proliferation of stimulated CTLL-2 mouse cytotoxic T cells. The ED50 this effect is 0.8023 μg/ml, corresponding to a specific activity is 1246.42 units/mg.

Assay Types
  • 1/16

    MedchemExpress Validation
     Western blot analysis of extracts from HepG2 (lane 2(20μg) and HepG2 (lane 3 (40μg) using Peroxiredoxin 5 Rabbit mAb. Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P80438, 1/10000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Mouse IgG-HRP Secondary Antibody (1/10000) was used for 1 hour at room temperature.

  • 1/16

    MedchemExpress Validation
     Immunohistochemical analysis of paraffin-embedded Mouse kidney tissue using Peroxiredoxin 5 Antibody (YA1591). The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked in QuickBlock for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HY-P81846, 1/100) in 4℃ overnight. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • 1/16

    MedchemExpress Validation
     Immunohistochemical analysis of paraffin-embedded Mouse kidney tissue using Peroxiredoxin 5 Antibody (YA1591). The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked in QuickBlock for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HY-P81846, 1/100) in 4℃ overnight. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • Peroxiredoxin 5 Antibody (YA1591)(PBS only) Product Image
    1/16
  • Experimental Validation Results for Peroxiredoxin 5 Antibody (YA1591)(PBS only)
  • Experimental Validation Results for Peroxiredoxin 5 Antibody (YA1591)(PBS only)
  • Experimental Validation Results for Peroxiredoxin 5 Antibody (YA1591)(PBS only)
  • Peroxiredoxin 5 Antibody (YA1591)(PBS only) Product Image
  • Experimental Validation Results for Peroxiredoxin 5 Antibody (YA1591)(PBS only)
    1/1
    MedchemExpress Validation
     Western blot analysis of extracts from HepG2 (lane 2(20μg) and HepG2 (lane 3 (40μg) using Peroxiredoxin 5 Rabbit mAb. Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P80438, 1/10000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Mouse IgG-HRP Secondary Antibody (1/10000) was used for 1 hour at room temperature.
  • Experimental Validation Results for Peroxiredoxin 5 Antibody (YA1591)(PBS only)
    1/1
    MedchemExpress Validation
     Immunohistochemical analysis of paraffin-embedded Mouse kidney tissue using Peroxiredoxin 5 Antibody (YA1591). The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked in QuickBlock for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HY-P81846, 1/100) in 4℃ overnight. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
  • Experimental Validation Results for Peroxiredoxin 5 Antibody (YA1591)(PBS only)
    1/1
    MedchemExpress Validation
     Immunohistochemical analysis of paraffin-embedded Mouse kidney tissue using Peroxiredoxin 5 Antibody (YA1591). The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked in QuickBlock for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HY-P81846, 1/100) in 4℃ overnight. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
  • Experimental Validation Results for Peroxiredoxin 5 Antibody (YA1591)(PBS only)
  • Experimental Validation Results for Peroxiredoxin 5 Antibody (YA1591)(PBS only)
  • Experimental Validation Results for Peroxiredoxin 5 Antibody (YA1591)(PBS only)
  • Peroxiredoxin 5 Antibody (YA1591)(PBS only) Product Image
    MedchemExpress Validation

    Western blot analysis of extracts from HepG2 (lane 2(20μg) and HepG2 (lane 3 (40μg) using Peroxiredoxin 5 Rabbit mAb. Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P80438, 1/10000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Mouse IgG-HRP Secondary Antibody (1/10000) was used for 1 hour at room temperature.

  • Peroxiredoxin 5 Antibody (YA1591)(PBS only) Product Image
    MedchemExpress Validation

    Immunohistochemical analysis of paraffin-embedded Mouse kidney tissue using Peroxiredoxin 5 Antibody (YA1591). The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked in QuickBlock for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HY-P81846, 1/100) in 4℃ overnight. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • Peroxiredoxin 5 Antibody (YA1591)(PBS only) Product Image
    MedchemExpress Validation

    Immunohistochemical analysis of paraffin-embedded Mouse kidney tissue using Peroxiredoxin 5 Antibody (YA1591). The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked in QuickBlock for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HY-P81846, 1/100) in 4℃ overnight. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • Peroxiredoxin 5 Antibody (YA1591)(PBS only) Product Image WB
  • Peroxiredoxin 5 Antibody (YA1591)(PBS only) Product Image IHC-P
  • Peroxiredoxin 5 Antibody (YA1591)(PBS only) Product Image IHC-P