B7H3/ICOSL Antibody (YA3064)(PBS only)

Cat. No.: HY-P83319A: Data Sheet User Guide for Antibodies
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B7H3/ICOSL Antibody (YA3064) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to B7H3/ICOSL.

For research use only. We do not sell to patients.

Size		Stock
50 μL		Get quote
100 μL		Get quote

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WB: Western Blot;
IHC-P: Immunohistochemistry-Paraffin;
IHC-F: Immunohistochemistry-Frozen;
ICC/IF: Immunocytochemistry/Immunofluorescence;
IF-Tissue: Immunofluorescence-Tissue;
mIHC: Multiplex Immunohistochemical;
IP: Immunoprecipitation;
ChIP: Chromatin Immunoprecipitation;
FC: Flow Cytometry;
ELISA: Enzyme Linked Immunosorbent Assay

Product Detail
Background
Documentation

Description

B7H3/ICOSL Antibody (YA3064) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to B7H3/ICOSL.

Host

Rabbit

Molecular Weight

Predicted band size: 33 kDa;

Observed band size: 75 kDa

Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.

Species Reactivity

Human

SwissProt ID

O75144

Gene ID

23308 [NCBI]

Immunogen

Recombinant protein of human ICOS Ligand

Application &
Dilution Ratio

Application	Dilution Ratio
WB WB: Western Blot	1:500-1:1000
IP IP: Immunoprecipitation	1:20

Purity	Affinity Purified	Conjugation	Non-conjugated
Isotype	IgG

Appearance

Liquid

Formulation

Supplied in PBS, pH 7.4.

Storage & Stability

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

Shipping

Shipping with blue ice.

Background

Function：Ligand for the T-cell-specific cell surface receptor ICOS. Acts as a costimulatory signal for T-cell proliferation and cytokine secretion (PubMed:11007762, PubMed:11023515, PubMed:30498080). Also induces B-cell proliferation and differentiation into plasma cells. Could play an important role in mediating local tissue responses to inflammatory conditions, as well as in modulating the secondary immune response by co-stimulating memory T-cell function (By similarity). In endothelial cells, required for proper neutrophil transmigration in response to chemoattractants, such as CXCL8/IL8 or N-formyl-methionyl peptides (fMLP) (PubMed:30498080)

Subcellular Localization：Cell membrane; Single-pass type I membrane protein; Cell membrane

Expression：
Tissue_specificity:It is expressed (protein level) on peripheral blood B cells and monocytes, as well as dendritic cells derived from monocytes; it is widely expressed in the brain, heart, kidney, liver, lung, pancreas, placenta, skeletal muscle, bone marrow, colon, ovary, prostate, testis, lymph nodes, leukocytes, spleen, thymus, and tonsils; it is detected only in lymph nodes, leukocytes, and spleen. It is expressed on activated monocytes and dendritic cells.

Induction:Constitutive expression is up-regulated by treatment with TNF in peripheral blood B-cells and monocytes, while it is decreased in dendritic cells (PubMed:11007762) . In monocytes, up-regulated by CCL3/macrophage inflammatory protein 1-alpha and IFNG (PubMed:10779774)

Isoforms & Post-Translational Modification：O75144 has 3 isomers: O75144-1: 33349 Da (predicted); O75144-2: 34225 Da (predicted); O75144-3: 20120 Da (predicted).

Subunit：Interacts with CTLA4 (in vitro)

Research Field

Immunology

Synonyms

inducible T-cell co-stimulator ligand; B7H2; GL50; B7-H2; B7RP1; CD275; ICOSL; LICOS; B7RP-1; ICOS-L

Documentation

User Guide for Antibodies (1077 KB)