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CD11c Antibody(YA3565)

(Synonyms: 95 alpha chain antibody; 95 antibody; CD 11c antibody; CD11 antigen like family member C antibody; CD11 antigen-like family member C antibody; CD11c antibody; CD11c antigen antibody; Complement component 3 receptor 4 subunit antibody; CR4 antibody; Integrin alpha X antibody; Integrin alpha X chain antibody; Integrin alpha-X antibody; Integrin aX antibody; Integrin subunit alpha X antibody; integrin, alpha X (antigen CD11C (p150), alpha polypeptide) antibody; integrin, alpha X (complement component 3 receptor 4 subunit antibody; ITAX_HUMAN antibody; ITGAX antibody; LEU M5 alpha subunit antibody; Leu M5 antibody)
Customer Review

Based on 1 Customer Validation

CD11c Antibody(YA3565) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to CD11c.

For research use only. We do not sell to patients.
  • Host:

    Rabbit

  • Isotype:

    IgG

  • Application:

    WB, IHC-P, IHC-F, IF-Tissue

  • Reactivity :

    Mouse

  • Formulation:

    Supplied in PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

  • Conjugation:
    Non-conjugated
ALL WB IHC-P
  • Experimental Validation Results for CD11c Antibody(YA3565)
  • Experimental Validation Results for CD11c Antibody(YA3565)
  • Experimental Validation Results for CD11c Antibody(YA3565)
  • CD11c Antibody(YA3565) Product Image
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To place orders, for customer services and technical support, please contact: MedChemExpress USA

Tel: 609-228-6898 E-mail: [email protected] [email protected]

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Data Download
Related Products:
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Use of MCE Products

Applications

Application
WB Info
WB: Western Blot
IHC-P Info
IHC-P: Immunohistochemistry-Paraffin
IHC-F Info
IHC-F: Immunohistochemistry-Frozen
IF-Tissue Info
IF-Tissue: Immunofluorescence-Tissue
Dilution Ratio 1:1000 1:1000 1:500-1:1000 1:200

Product Details

Description

CD11c Antibody(YA3565) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to CD11c.

  • Host Rabbit
  • Clonality Recombinant, Monoclonal
  • Species Reactivity
    Mouse
  • Observed Molecular Weight
    Observed band size: 140 kDa Info
    Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.
  • Calculated Molecular Weight Predicted band size: 129 kDa
Immunogen

Recombinant protein within mouse CD11c aa 616-936 / 1,169.

Purification

Affinity Purified

Conjugation

Non-conjugated

Modification

Unmodified

Isotype

IgG

Product Properties

  • Appearance

    Liquid

  • Formulation

    Supplied in PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

  • Concentration

    Batch-dependent, Please check the COA for the concentration of each lot. Check Lot Concentration

  • Storage & Stability

    Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

  • Shipping

    Shipping with blue ice.

Verification Images

  • Experimental Validation Results for CD11c Antibody(YA3565)
    Western blot analysis of extracts from RAW264.7(lane 2(20ug) and RAW264.7(lane 3(40ug) using CD11c Antibody (HY-P83768) Rabbit mAb. Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P83730, 1/10000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (1/10000) was used for 1 hour at room temperature.
  • Experimental Validation Results for CD11c Antibody(YA3565)
    Immunohistochemical analysis of paraffin-embedded mouse spleen tissue using CD11c Antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked in QuickBlock for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody at 1/100 dilution in 4℃ overnight. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
  • Experimental Validation Results for CD11c Antibody(YA3565)
    Immunohistochemical analysis of paraffin-embedded mouse spleen tissue using CD11c Antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked in QuickBlock for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody at 1/100 dilution in 4℃ overnight. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Background

  • Function

    CD11c is an integrin α-chain protein, belonging to the integrin α subunit family. CD11c usually forms a heterodimer αXβ2 integrin with CD18 (Integrin β2, ITGB2). CD11c is one of the most commonly used dendritic cell (DC) markers in immunological research, but it is not actually expressed specifically by DCs; it is expressed in various myeloid immune cells.

  • Subcellular Localization

    Membrane; Single-pass type I membrane protein

  • Expression


    Tissue_specificity:It is mainly expressed in monocytes and granulocytes.

  • Subunit

    Heterodimer of an alpha and a beta subunit. Alpha-X associates with beta-2

  • SwissProt ID

    Q9QXH4

  • Gene ID
    16411 [NCBI]
  • Synonyms

    95 alpha chain antibody; 95 antibody; CD 11c antibody; CD11 antigen like family member C antibody; CD11 antigen-like family member C antibody; CD11c antibody; CD11c antigen antibody; Complement component 3 receptor 4 subunit antibody; CR4 antibody; Integrin alpha X antibody; Integrin alpha X chain antibody; Integrin alpha-X antibody; Integrin aX antibody; Integrin subunit alpha X antibody; integrin, alpha X (antigen CD11C (p150), alpha polypeptide) antibody; integrin, alpha X (complement component 3 receptor 4 subunit antibody; ITAX_HUMAN antibody; ITGAX antibody; LEU M5 alpha subunit antibody; Leu M5 antibody

CD11c Antibody(YA3565) Related Classifications

MOQ
Minimum order quantity
100 mg

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Measured by its ability to inhibit anti-CD3-induced proliferation of stimulated CTLL-2 mouse cytotoxic T cells. The ED50 this effect is 0.8023 μg/ml, corresponding to a specific activity is 1246.42 units/mg.

Assay Types
  • 1/16

    MedchemExpress Validation
     Western blot analysis of extracts from RAW264.7(lane 2(20ug) and RAW264.7(lane 3(40ug) using CD11c Antibody (HY-P83768) Rabbit mAb. Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P83730, 1/10000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (1/10000) was used for 1 hour at room temperature.

  • 1/16

    MedchemExpress Validation
     Immunohistochemical analysis of paraffin-embedded mouse spleen tissue using CD11c Antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked in QuickBlock for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody at 1/100 dilution in 4℃ overnight. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • 1/16

    MedchemExpress Validation
     Immunohistochemical analysis of paraffin-embedded mouse spleen tissue using CD11c Antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked in QuickBlock for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody at 1/100 dilution in 4℃ overnight. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • CD11c Antibody(YA3565) Product Image
    1/16
  • Experimental Validation Results for CD11c Antibody(YA3565)
  • Experimental Validation Results for CD11c Antibody(YA3565)
  • Experimental Validation Results for CD11c Antibody(YA3565)
  • CD11c Antibody(YA3565) Product Image
  • Experimental Validation Results for CD11c Antibody(YA3565)
    1/1
    MedchemExpress Validation
     Western blot analysis of extracts from RAW264.7(lane 2(20ug) and RAW264.7(lane 3(40ug) using CD11c Antibody (HY-P83768) Rabbit mAb. Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P83730, 1/10000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (1/10000) was used for 1 hour at room temperature.
  • Experimental Validation Results for CD11c Antibody(YA3565)
    1/1
    MedchemExpress Validation
     Immunohistochemical analysis of paraffin-embedded mouse spleen tissue using CD11c Antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked in QuickBlock for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody at 1/100 dilution in 4℃ overnight. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
  • Experimental Validation Results for CD11c Antibody(YA3565)
    1/1
    MedchemExpress Validation
     Immunohistochemical analysis of paraffin-embedded mouse spleen tissue using CD11c Antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked in QuickBlock for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody at 1/100 dilution in 4℃ overnight. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
  • Experimental Validation Results for CD11c Antibody(YA3565)
  • Experimental Validation Results for CD11c Antibody(YA3565)
  • Experimental Validation Results for CD11c Antibody(YA3565)
  • CD11c Antibody(YA3565) Product Image
    MedchemExpress Validation

    Western blot analysis of extracts from RAW264.7(lane 2(20ug) and RAW264.7(lane 3(40ug) using CD11c Antibody (HY-P83768) Rabbit mAb. Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P83730, 1/10000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (1/10000) was used for 1 hour at room temperature.

  • CD11c Antibody(YA3565) Product Image
    MedchemExpress Validation

    Immunohistochemical analysis of paraffin-embedded mouse spleen tissue using CD11c Antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked in QuickBlock for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody at 1/100 dilution in 4℃ overnight. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • CD11c Antibody(YA3565) Product Image
    MedchemExpress Validation

    Immunohistochemical analysis of paraffin-embedded mouse spleen tissue using CD11c Antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked in QuickBlock for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody at 1/100 dilution in 4℃ overnight. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • CD11c Antibody(YA3565) Product Image WB
  • CD11c Antibody(YA3565) Product Image IHC-P
  • CD11c Antibody(YA3565) Product Image IHC-P