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YTHDF1 Antibody (YA1429)

(Synonyms: C20orf21; DF1)
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Based on 1 Customer Validation

YTHDF1 Antibody (YA1429) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to YTHDF1.

For research use only. We do not sell to patients.
  • Host:

    Rabbit

  • Isotype:

    IgG

  • Application:

    WB, IP

  • Reactivity :

    Human, Hamster

  • Formulation:

    Supplied in 50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40% Glycerol, 0.01% Sodium azide and 0.05% BSA

  • Conjugation:
    Non-conjugated
ALL WB ICC
  • Experimental Validation Results for YTHDF1 Antibody (YA1429)
  • Experimental Validation Results for YTHDF1 Antibody (YA1429)
  • Experimental Validation Results for YTHDF1 Antibody (YA1429)
  • YTHDF1 Antibody (YA1429) Product Image
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Tel: 609-228-6898 E-mail: [email protected] [email protected]

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Use of MCE Products

Applications

Application
WB Info
WB: Western Blot
IP Info
IP: Immunoprecipitation
Dilution Ratio 1:500-1:1000 1:20

Product Details

Description

YTHDF1 Antibody (YA1429) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to YTHDF1.

  • Host Rabbit
  • Clonality Recombinant,Monoclonal
  • Species Reactivity
    Human, Hamster
  • Observed Molecular Weight
    Observed band size: 72 kDa Info
    Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.
  • Calculated Molecular Weight Predicted band size: 61 kDa
Species Reactivity Database

Entrez Gene: 54915 Human ;

SwissProt: Q9BYJ9 Human ;

Immunogen

Recombinant protein of mouse YTHDF1

Sensitivity

Endogenous

Purification

Affinity Purified

Conjugation

Non-conjugated

Modification

Unmodified

Isotype

IgG

RRID

AB_3103792

Product Properties

  • Appearance

    Solution

  • Formulation

    Supplied in 50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40% Glycerol, 0.01% Sodium azide and 0.05% BSA

  • Concentration

    Batch-dependent, Please check the COA for the concentration of each lot. Check Lot Concentration

  • Storage & Stability

    Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

  • Shipping

    Shipping with blue ice.

Verification Images

  • Experimental Validation Results for YTHDF1 Antibody (YA1429)
    Western blot analysis of extracts from HepG2(lane 2(20ug) , Hela(lane 3(20ug) and NIH/3T3(lane 4(20ug) using YTHDF1 Antibody (HY-P81684) Rabbit mAb. Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P80438, 1/10000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (1/10000) was used for 1 hour at room temperature.
  • Experimental Validation Results for YTHDF1 Antibody (YA1429)
    Immunocytochemistry analysis of NIH3T3 cells labeling YTHDF1 with YTHDF1 Antibody (HY-P81684) at 1/50 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 for 10 minutes at room temperature, then blocked with QuickBlock™ Blocking Buffer for Immunol Staining for 10 min at room temperature. Cells were then incubated with YTHDF1 Antibody (HY-P81684) at 1/50 dilution in QuickBlock™ Blocking Buffer for Immunol Staining at 4 ℃. Alexa Fluor® 488-conjugated AffiniPure Goat Anti-Rabbit IgG H&L（HY-P8002, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).
  • Experimental Validation Results for YTHDF1 Antibody (YA1429)
    Immunocytochemistry analysis of NIH3T3 cells labeling YTHDF1 with YTHDF1 Antibody (HY-P81684) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 for 10 minutes at room temperature, then blocked with QuickBlock™ Blocking Buffer for Immunol Staining for 10 min at room temperature. Cells were then incubated with YTHDF1 Antibody (HY-P81684)at 1/100 dilution in QuickBlock™ Blocking Buffer for Immunol Staining at 4 ℃. Alexa Fluor® 488-conjugated AffiniPure Goat Anti-Rabbit IgG H&L（HY-P8002,Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).

Background

  • Function

    YTHDF1 (YTH N6-methyladenosine RNA Binding Protein 1) is a major m6A reader that recognizes N6-methyladenosine-modified transcripts and promotes the translation of methylated mRNAs, thereby regulating post-transcriptional gene expression and protein synthesis.[1][4] Mechanistically, YTHDF1 enhances the translational efficiency of its target transcripts through interactions with the translation machinery and translation initiation factors, making it a central effector of m6A-dependent gene regulation.[1][5][6] Beyond its role in RNA metabolism, YTHDF1 participates in immune regulation, where m6A-dependent YTHDF1 activity controls antigen-related responses and contributes to antitumor immunity pathways.[5] In disease contexts, elevated YTHDF1 expression has been reported across multiple malignancies, including ovarian, gastric, colorectal, hepatocellular, and lung cancers, where it promotes tumor growth, metastasis, stemness, or therapeutic resistance through enhanced translation of oncogenic targets.[1][2][3][9][10] Representative downstream effectors include EIF3C, USP14, FZD7, and YAP, which link YTHDF1-mediated translational control to cancer progression and cellular adaptation.[1][2][3][9] Compared with related YTH-domain family members, YTHDF1 is primarily characterized by its translation-promoting activity, whereas YTHDF2 is widely associated with mRNA decay and degradation pathways, highlighting a functional distinction frequently exploited in mechanistic studies of m6A signaling.[5][11] Consequently, genetic silencing or functional inhibition of YTHDF1 is widely used in experimental models to investigate m6A-dependent translation, tumor biology, immune regulation, and therapeutic target validation.[5][10]

  • Subcellular Localization

    Cytoplasm; Cytoplasm, P-body; Cytoplasm, Stress granule

  • Expression


    Tissue_specificity:In brain, preferentially expressed in the hippocampus

  • Subunit

    Interacts with CNOT1; promoting recruitment of the CCR4-NOT complex (By similarity). Interacts with ribosomes (By similarity). Interacts with eIF3 (EIF3A or EIF3B). Interacts with YTHDF3 (By similarity)

  • SwissProt ID

    P59326

  • Gene ID
    54915 [NCBI]
  • Synonyms

    C20orf21; DF1

  • Research Field

    Epigenetics and Nuclear Signaling

[1]. Siehler S, et al. Characterisation of human recombinant somatostatin receptors. 3. Modulation of adenylate cyclase activity. Naunyn Schmiedebergs Arch Pharmacol. 1999 Nov;360(5):510-21. [Content Brief]

[2]. Smalley KS, et al. The pivotal role of phosphoinositide-3 kinase in the human somatostatin sst(4) receptor-mediated stimulation of p44/p42 mitogen-activated protein kinase and extracellular acidification. Biochem Biophys Res Commun. 1999 Sep 16;263(1):239-43. [Content Brief]

[3]. Xu Y, et al. Molecular cloning and sequencing of a human somatostatin receptor, hSSTR4. Biochem Biophys Res Commun. 1993 Jun 15;193(2):648-52. [Content Brief]

[4]. Liu T, et al. The m6A reader YTHDF1 promotes ovarian cancer progression via augmenting EIF3C translation. Nucleic Acids Res. 2020 Apr 17;48(7):3816-3831. [Content Brief]

[5]. Drago SFA, et al. Effects of a Dietary Supplement Composed of Baicalin, Bromelain and Escin for Venous Chronic Insufficiency Treatment: Insights from a Retrospective Observational Study. Pharmaceuticals (Basel). 2024 Jun 14;17(6):779. [Content Brief]

[6]. Pi J, et al. YTHDF1 Promotes Gastric Carcinogenesis by Controlling Translation of FZD7. Cancer Res. 2021 May 15;81(10):2651-2665. [Content Brief]

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YTHDF1 Antibody (YA1429) Related Classifications

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100 mg

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Measured by its ability to inhibit anti-CD3-induced proliferation of stimulated CTLL-2 mouse cytotoxic T cells. The ED50 this effect is 0.8023 μg/ml, corresponding to a specific activity is 1246.42 units/mg.

Assay Types
  • 1/16

    MedchemExpress Validation
     Western blot analysis of extracts from HepG2(lane 2(20ug) , Hela(lane 3(20ug) and NIH/3T3(lane 4(20ug) using YTHDF1 Antibody (HY-P81684) Rabbit mAb. Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P80438, 1/10000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (1/10000) was used for 1 hour at room temperature.

  • 1/16

    MedchemExpress Validation
     Immunocytochemistry analysis of NIH3T3 cells labeling YTHDF1 with YTHDF1 Antibody (HY-P81684) at 1/50 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 for 10 minutes at room temperature, then blocked with QuickBlock™ Blocking Buffer for Immunol Staining for 10 min at room temperature. Cells were then incubated with YTHDF1 Antibody (HY-P81684) at 1/50 dilution in QuickBlock™ Blocking Buffer for Immunol Staining at 4 ℃. Alexa Fluor® 488-conjugated AffiniPure Goat Anti-Rabbit IgG H&L（HY-P8002, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).

  • 1/16

    MedchemExpress Validation
     Immunocytochemistry analysis of NIH3T3 cells labeling YTHDF1 with YTHDF1 Antibody (HY-P81684) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 for 10 minutes at room temperature, then blocked with QuickBlock™ Blocking Buffer for Immunol Staining for 10 min at room temperature. Cells were then incubated with YTHDF1 Antibody (HY-P81684)at 1/100 dilution in QuickBlock™ Blocking Buffer for Immunol Staining at 4 ℃. Alexa Fluor® 488-conjugated AffiniPure Goat Anti-Rabbit IgG H&L（HY-P8002,Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).

  • YTHDF1 Antibody (YA1429) Product Image
    1/16
  • Experimental Validation Results for YTHDF1 Antibody (YA1429)
  • Experimental Validation Results for YTHDF1 Antibody (YA1429)
  • Experimental Validation Results for YTHDF1 Antibody (YA1429)
  • YTHDF1 Antibody (YA1429) Product Image
  • Experimental Validation Results for YTHDF1 Antibody (YA1429)
    1/1
    MedchemExpress Validation
     Western blot analysis of extracts from HepG2(lane 2(20ug) , Hela(lane 3(20ug) and NIH/3T3(lane 4(20ug) using YTHDF1 Antibody (HY-P81684) Rabbit mAb. Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P80438, 1/10000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (1/10000) was used for 1 hour at room temperature.
  • Experimental Validation Results for YTHDF1 Antibody (YA1429)
    1/1
    MedchemExpress Validation
     Immunocytochemistry analysis of NIH3T3 cells labeling YTHDF1 with YTHDF1 Antibody (HY-P81684) at 1/50 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 for 10 minutes at room temperature, then blocked with QuickBlock™ Blocking Buffer for Immunol Staining for 10 min at room temperature. Cells were then incubated with YTHDF1 Antibody (HY-P81684) at 1/50 dilution in QuickBlock™ Blocking Buffer for Immunol Staining at 4 ℃. Alexa Fluor® 488-conjugated AffiniPure Goat Anti-Rabbit IgG H&L（HY-P8002, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).
  • Experimental Validation Results for YTHDF1 Antibody (YA1429)
    1/1
    MedchemExpress Validation
     Immunocytochemistry analysis of NIH3T3 cells labeling YTHDF1 with YTHDF1 Antibody (HY-P81684) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 for 10 minutes at room temperature, then blocked with QuickBlock™ Blocking Buffer for Immunol Staining for 10 min at room temperature. Cells were then incubated with YTHDF1 Antibody (HY-P81684)at 1/100 dilution in QuickBlock™ Blocking Buffer for Immunol Staining at 4 ℃. Alexa Fluor® 488-conjugated AffiniPure Goat Anti-Rabbit IgG H&L（HY-P8002,Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).
  • Experimental Validation Results for YTHDF1 Antibody (YA1429)
  • Experimental Validation Results for YTHDF1 Antibody (YA1429)
  • Experimental Validation Results for YTHDF1 Antibody (YA1429)
  • YTHDF1 Antibody (YA1429) Product Image
    MedchemExpress Validation

    Western blot analysis of extracts from HepG2(lane 2(20ug) , Hela(lane 3(20ug) and NIH/3T3(lane 4(20ug) using YTHDF1 Antibody (HY-P81684) Rabbit mAb. Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P80438, 1/10000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (1/10000) was used for 1 hour at room temperature.

  • YTHDF1 Antibody (YA1429) Product Image
    MedchemExpress Validation

    Immunocytochemistry analysis of NIH3T3 cells labeling YTHDF1 with YTHDF1 Antibody (HY-P81684) at 1/50 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 for 10 minutes at room temperature, then blocked with QuickBlock™ Blocking Buffer for Immunol Staining for 10 min at room temperature. Cells were then incubated with YTHDF1 Antibody (HY-P81684) at 1/50 dilution in QuickBlock™ Blocking Buffer for Immunol Staining at 4 ℃. Alexa Fluor® 488-conjugated AffiniPure Goat Anti-Rabbit IgG H&L（HY-P8002, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).

  • YTHDF1 Antibody (YA1429) Product Image
    MedchemExpress Validation

    Immunocytochemistry analysis of NIH3T3 cells labeling YTHDF1 with YTHDF1 Antibody (HY-P81684) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 for 10 minutes at room temperature, then blocked with QuickBlock™ Blocking Buffer for Immunol Staining for 10 min at room temperature. Cells were then incubated with YTHDF1 Antibody (HY-P81684)at 1/100 dilution in QuickBlock™ Blocking Buffer for Immunol Staining at 4 ℃. Alexa Fluor® 488-conjugated AffiniPure Goat Anti-Rabbit IgG H&L（HY-P8002,Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).

  • YTHDF1 Antibody (YA1429) Product Image WB
  • YTHDF1 Antibody (YA1429) Product Image ICC
  • YTHDF1 Antibody (YA1429) Product Image ICC