EphB2 Antibody (YA4838)

Cat. No.: HY-P85146: Data Sheet User Guide for Antibodies
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EphB2 Antibody (YA4838) is a Mouse-derived and non-conjugated monoclonal antibody, targeting to EphB2.

For research use only. We do not sell to patients.

Size		Stock
50 μL		Get quote
100 μL		Get quote

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WB: Western Blot;
IHC-P: Immunohistochemistry-Paraffin;
IHC-F: Immunohistochemistry-Frozen;
ICC/IF: Immunocytochemistry/Immunofluorescence;
IF-Tissue: Immunofluorescence-Tissue;
mIHC: Multiplex Immunohistochemical;
IP: Immunoprecipitation;
ChIP: Chromatin Immunoprecipitation;
FC: Flow Cytometry;
ELISA: Enzyme Linked Immunosorbent Assay

Product Detail
Background
Documentation

Description

EphB2 Antibody (YA4838) is a Mouse-derived and non-conjugated monoclonal antibody, targeting to EphB2.

Host

Mouse

Clonality

Monoclonal

Molecular Weight

Predicted band size: 117 kDa;

Species Reactivity

Human

SwissProt ID

P29323

Gene ID

2048 [NCBI]

Immunogen

Purified recombinant fragment of EphB2 (aa17-200) expressed in E. Coli.

Application &
Dilution Ratio

Application	Dilution Ratio
WB WB: Western Blot	1:500-1:2000
ICC/IF ICC/IF: Immunocytochemistry/Immunofluorescence	1:200-1:1000
ELISA ELISA: Enzyme Linked Immunosorbent Assay	1:10000

Purity	affinity purified.	Conjugation	Non-conjugated
Modification	Unmodified

Appearance

Liquid

Formulation

Supplied in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

Storage & Stability

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

Shipping

Shipping with blue ice.

Background

Function：Receptor tyrosine kinase which binds promiscuously transmembrane ephrin-B family ligands residing on adjacent cells, leading to contact-dependent bidirectional signaling into neighboring cells. The signaling pathway downstream of the receptor is referred to as forward signaling while the signaling pathway downstream of the ephrin ligand is referred to as reverse signaling. Functions in axon guidance during development. Involved in the guidance of commissural axons, that form a major interhemispheric connection between the 2 temporal lobes of the cerebral cortex. Also involved in guidance of contralateral inner ear efferent growth cones at the midline and of retinal ganglion cell axons to the optic disk. In addition to axon guidance, also regulates dendritic spines development and maturation and stimulates the formation of excitatory synapses. Upon activation by EFNB1, abolishes the ARHGEF15-mediated negative regulation on excitatory synapse formation. Controls other aspects of development including angiogenesis, palate development and in inner ear development through regulation of endolymph production. Forward and reverse signaling through the EFNB2/EPHB2 complex regulate movement and adhesion of cells that tubularize the urethra and septate the cloaca. May function as a tumor suppressor. May be involved in the regulation of platelet activation and blood coagulation (PubMed:30213874)

Subcellular Localization：Cell membrane; Single-pass type I membrane protein; Cell projection, axon; Cell projection, dendrite

Expression：
Tissue_specificity:Brain, heart, lungs, kidneys, placenta, pancreas, liver, and skeletal muscle. It is preferentially expressed in the fetal brain.

Isoforms & Post-Translational Modification：P29323 has 3 isomers: P29323-1: 117493 Da (predicted); P29323-2: 109874 Da (predicted); P29323-3: 110030 Da (predicted).
Autophosphorylated; ligand binding stimulates autophosphorylation on tyrosine residues;Polyubiquitinated; ligand binding stimulates ubiquitination (PubMed:28931592). Ubiquitinated by RNF186 at Lys-891, mainly through 'Lys-27'-linked polyubiquitin chains (PubMed:33280498). Ubiquitinated by CRL2(KLHDC2) E3 ligase complex (PubMed:36805027);Ligand binding induces cleavage by matrix metalloproteinases (MMPs) such as MMP7/MMP9, producing an EphB2/N-terminal fragment (NTF) and a C-terminal long fragment (EphB2-LF). EphB2-LF is further cleaved by MMPs, producing EphB2/CTF1 which is further cleaved by the PS1/gamma-secretase producing EphB2/CTF2

Subunit：The heterotetramer is composed of an ephrin dimer and a receptor dimer (PubMed:17897949).

Synonyms

EPHB2; DRT; EPHT3; EPTH3; ERK; HEK5; TYRO5; Ephrin type-B receptor 2; Developmentally-regulated Eph-related tyrosine kinase; ELK-related tyrosine kinase; EPH tyrosine kinase 3; EPH-like kinase 5; EK5; hEK5; Renal carcinoma antigen NY-REN-47

Documentation

User Guide for Antibodies (1077 KB)