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BRMS1 Antibody (YA6965)

(Synonyms: AV003220 antibody; AW554636 antibody; Breast cancer metastasis suppressor 1 antibody; Breast cancer metastasis-suppressor 1 antibody; BRMS1 antibody; BRMS1_HUMAN antibody; DKFZp564A063 antibody; MGC95128 antibody)
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Based on 1 Customer Validation

BRMS1 Antibody (YA6965) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to BRMS1.

For research use only. We do not sell to patients.
  • Host:

    Rabbit

  • Isotype:

    IgG

  • Application:

    WB, ICC/IF, IHC-P

  • Reactivity :

    Human, Mouse, Rat

  • Formulation:

    Supplied in 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

  • Conjugation:
    Non-conjugated
  • Experimental Validation Results for BRMS1 Antibody (YA6965)
  • BRMS1 Antibody (YA6965) Product Image
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To place orders, for customer services and technical support, please contact: MedChemExpress USA

Tel: 609-228-6898 E-mail: [email protected] [email protected]

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Use of MCE Products

Applications

Application
WB Info
WB: Western Blot
ICC/IF Info
ICC/IF: Immunocytochemistry/
Immunofluorescence
IHC-P Info
IHC-P: Immunohistochemistry-Paraffin
Dilution Ratio 1:2000 1:50-1:100 1:200-1:1000

Product Details

Description

BRMS1 Antibody (YA6965) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to BRMS1.

  • Host Rabbit
  • Clonality Recombinant,Monoclonal
  • Species Reactivity
    Human, Mouse, Rat
  • Observed Molecular Weight
    Observed band size: 30 kDa Info
    Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.
  • Calculated Molecular Weight Predicted band size: 28 kDa
Immunogen

Recombinant protein within human BRMS1 aa 1-100.

Sensitivity

Endogenous

Purification

Protein A affinity purified.

Conjugation

Non-conjugated

Modification

Unmodified

Isotype

IgG

Product Properties

  • Appearance

    Liquid

  • Formulation

    Supplied in 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

  • Concentration

    Batch-dependent, Please check the COA for the concentration of each lot. Check Lot Concentration

  • Storage & Stability

    Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

  • Shipping

    Shipping with blue ice.

Verification Images

  • Experimental Validation Results for BRMS1 Antibody (YA6965)
    Western blot analysis of extracts from HeLa (lane 1(20μg)) 、HEK293 (lane 2(20μg)) 、Mouse testis (lane 3(20μg)) and Rat kidney (lane 4(20μg)) using BRMS1 Antibody (HY-P87282) . Proteins were transferred to a PVDF membrane and blocked with 5% nonfat dry milk in TBST for 1.5 hour at room temperature. The primary antibody (1/2000) and Loading control antibody (β Tubulin, 1/10000) was used in 5% nonfat dry milk in TBST at 4℃ overnight. Goat Anti-Rabbit IgG-HRP Secondary Antibody (1/10,000) was used for 1 hour at room temperature.

Background

  • Function

    BRMS1 transcriptional repressor. Down-regulates transcription activation by NF-kappa-B by promoting the deacetylation of RELA at 'Lys-310'. Promotes HDAC1 binding to promoter regions. Down-regulates expression of anti-apoptotic genes that are controlled by NF-kappa-B. Promotes apoptosis in cells that have inadequate adherence to a substrate, a process called anoikis, and may thereby inhibit metastasis. May be a mediator of metastasis suppression in breast carcinoma

  • Subcellular Localization

    Nucleus,Cytoplasm

  • Expression


    Tissue_Specificity: Expression levels are higher in term placentas than in early placentas. Low levels of expression observed in normal pregnancies and in molar pregnancies

  • Isoforms & Post-Translational Modification

    Q9HCU9: 246 amino acids, molecular weight 28461 Da.

  • Subunit

    Homohexamer (Potential)

  • SwissProt ID

    Q9HCU9

  • Synonyms

    AV003220 antibody; AW554636 antibody; Breast cancer metastasis suppressor 1 antibody; Breast cancer metastasis-suppressor 1 antibody; BRMS1 antibody; BRMS1_HUMAN antibody; DKFZp564A063 antibody; MGC95128 antibody

BRMS1 Antibody (YA6965) Related Classifications

MOQ
Minimum order quantity
100 mg

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Measured by its ability to inhibit anti-CD3-induced proliferation of stimulated CTLL-2 mouse cytotoxic T cells. The ED50 this effect is 0.8023 μg/ml, corresponding to a specific activity is 1246.42 units/mg.

Assay Types
  • 1/16

    MedChemExpress Validation
     Western blot analysis of extracts from HeLa (lane 1(20μg)) 、HEK293 (lane 2(20μg)) 、Mouse testis (lane 3(20μg)) and Rat kidney (lane 4(20μg)) using BRMS1 Antibody (HY-P87282) . Proteins were transferred to a PVDF membrane and blocked with 5% nonfat dry milk in TBST for 1.5 hour at room temperature. The primary antibody (1/2000) and Loading control antibody (β Tubulin, 1/10000) was used in 5% nonfat dry milk in TBST at 4℃ overnight. Goat Anti-Rabbit IgG-HRP Secondary Antibody (1/10,000) was used for 1 hour at room temperature.

  • BRMS1 Antibody (YA6965) Product Image
    1/16
  • Experimental Validation Results for BRMS1 Antibody (YA6965)
  • BRMS1 Antibody (YA6965) Product Image
  • Experimental Validation Results for BRMS1 Antibody (YA6965)
    1/1
    MedChemExpress Validation
     Western blot analysis of extracts from HeLa (lane 1(20μg)) 、HEK293 (lane 2(20μg)) 、Mouse testis (lane 3(20μg)) and Rat kidney (lane 4(20μg)) using BRMS1 Antibody (HY-P87282) . Proteins were transferred to a PVDF membrane and blocked with 5% nonfat dry milk in TBST for 1.5 hour at room temperature. The primary antibody (1/2000) and Loading control antibody (β Tubulin, 1/10000) was used in 5% nonfat dry milk in TBST at 4℃ overnight. Goat Anti-Rabbit IgG-HRP Secondary Antibody (1/10,000) was used for 1 hour at room temperature.
  • Experimental Validation Results for BRMS1 Antibody (YA6965)
  • BRMS1 Antibody (YA6965) Product Image
    MedChemExpress Validation

    Western blot analysis of extracts from HeLa (lane 1(20μg)) 、HEK293 (lane 2(20μg)) 、Mouse testis (lane 3(20μg)) and Rat kidney (lane 4(20μg)) using BRMS1 Antibody (HY-P87282) . Proteins were transferred to a PVDF membrane and blocked with 5% nonfat dry milk in TBST for 1.5 hour at room temperature. The primary antibody (1/2000) and Loading control antibody (β Tubulin, 1/10000) was used in 5% nonfat dry milk in TBST at 4℃ overnight. Goat Anti-Rabbit IgG-HRP Secondary Antibody (1/10,000) was used for 1 hour at room temperature.

  • BRMS1 Antibody (YA6965) Product Image WB