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SOCS-3 Antibody (YA3498)

(Synonyms: CIS3; SSI3; ATOD4; Cish3; SSI-3; SOCS-3)
Revisión del cliente

Based on 1 Customer Validation

SOCS-3 Antibody (YA3498) is a Mouse-derived and non-conjugated IgG1 monoclonal antibody, targeting to SOCS-3.

For research use only. We do not sell to patients.
  • Host:

    Mouse

  • Isotype:

    IgG

  • Application:

    WB, IHC-P, ICC/IF, FC, ELISA

  • Reactivity :

    Human

  • Formulation:

    Supplied in PBS with 0.05% sodium azide

  • Conjugation:
    Non-conjugated
ALL WB IHC-P ICC
  • Experimental Validation Results for SOCS-3 Antibody (YA3498)
  • Experimental Validation Results for SOCS-3 Antibody (YA3498)
  • Experimental Validation Results for SOCS-3 Antibody (YA3498)
  • Experimental Validation Results for SOCS-3 Antibody (YA3498)
  • Experimental Validation Results for SOCS-3 Antibody (YA3498)
  • Experimental Validation Results for SOCS-3 Antibody (YA3498)
  • Experimental Validation Results for SOCS-3 Antibody (YA3498)
  • Experimental Validation Results for SOCS-3 Antibody (YA3498)
  • Experimental Validation Results for SOCS-3 Antibody (YA3498)
  • SOCS-3 Antibody (YA3498) Product Image
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To place orders, for customer services and technical support, please contact: MedChemExpress USA

Tel: 609-228-6898 E-mail: [email protected] [email protected]

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Use of MCE Products

Applications

Application
WB Info
WB: Western Blot
IHC-P Info
IHC-P: Immunohistochemistry-Paraffin
ICC/IF Info
ICC/IF: Immunocytochemistry/
Immunofluorescence
FC Info
FC: Flow Cytometry
ELISA Info
ELISA: Enzyme Linked Immunosorbent Assay
Dilution Ratio 1:500-1:2000 1:200-1:1000 1:200-1:1000 1:200-1:400 1:10000

Product Details

Descripciòn

SOCS-3 Antibody (YA3498) is a Mouse-derived and non-conjugated IgG1 monoclonal antibody, targeting to SOCS-3.

  • Host Mouse
  • Clonality Monoclonal
  • Species Reactivity
    Human
  • Observed Molecular Weight
    Observed band size: 30 kDa Info
    Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.
  • Calculated Molecular Weight Predicted band size: 25 kDa
Immunogen

Purified recombinant fragment of human SOCS3 (AA: full(1-225)) expressed in E. Coli.

Purification

affinity purified.

Conjugation

Non-conjugated

Modification

Unmodified

Isotype

IgG

Product Properties

  • Appearance

    Solution

  • Formulation

    Supplied in PBS with 0.05% sodium azide

  • Concentration

    Batch-dependent, Please check the COA for the concentration of each lot. Check Lot Concentration

  • Storage & Stability

    Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

  • Envío

    Shipping with blue ice.

Verification Images

  • Experimental Validation Results for SOCS-3 Antibody (YA3498)
    Western blot analysis of extracts from PANC-1(lane 1(40μg) ) using SOCS3 antibody. Proteins were transferred to a NC membrane and blocked with 5% Skim milk in TBST for 2 hour at room temperature. The primary antibody ( 1/1000) and Loading control antibody (GAPDH， 1/1000) was used in 5% Skim milk in TBST at 4°C overnight. Goat Anti-Mouse IgG-HRP Secondary Antibody (1/10,000) was used for 1 hour at room temperature.
  • Experimental Validation Results for SOCS-3 Antibody (YA3498)
    Immunohistochemical analysis of paraffin-embedded human bronchus tissue using SOCS-3 Antibody (HY-P83801, 1/1000). The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
  • Experimental Validation Results for SOCS-3 Antibody (YA3498)
    Immunohistochemical analysis of paraffin-embedded human testis tissue using SOCS-3 Antibody (HY-P83801, 1/1000). The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
  • Experimental Validation Results for SOCS-3 Antibody (YA3498)
    Immunohistochemical analysis of paraffin-embedded human epididymis tissue using SOCS-3 Antibody (HY-P83801, 1/1000). The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
  • Experimental Validation Results for SOCS-3 Antibody (YA3498)
    Immunohistochemical analysis of paraffin-embedded human salivary gland tissue using SOCS-3 Antibody (HY-P83801, 1/1000). The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
  • Experimental Validation Results for SOCS-3 Antibody (YA3498)
    Immunohistochemical analysis of paraffin-embedded human kidney tissue using SOCS-3 Antibody (HY-P83801, 1/1000). The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
  • Experimental Validation Results for SOCS-3 Antibody (YA3498)
    Immunohistochemical analysis of paraffin-embedded human duodenum tissue using SOCS-3 Antibody (HY-P83801, 1/1000). The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
  • Experimental Validation Results for SOCS-3 Antibody (YA3498)
    Immunohistochemical analysis of paraffin-embedded human seminal vesicle tissue using SOCS-3 Antibody (HY-P83801, 1/1000). The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
  • Experimental Validation Results for SOCS-3 Antibody (YA3498)
    Immunofluorescence analysis of A549 cells labeling SOCS3 antibody at 1/50 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature.Cells were then incubated with SOCS3 antibody at 1/50 dilution in 1% BSA in PBST overnight at 4 ℃. AF 488 Goat Anti-mouse IgG H&L (green) was used as the secondary antibody at 1/500 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue)

Background

  • Function

    SOCS-3 is a SOCS family proteins form part of a classical negative feedback system that regulates cytokine signal transduction. SOCS3 is involved in negative regulation of cytokines that signal through the JAK/STAT pathway. Inhibits cytokine signal transduction by binding to tyrosine kinase receptors including IL6ST/gp130, LIF, erythropoietin, insulin, IL12, GCSF and leptin receptors. Binding to JAK2 inhibits its kinase activity and regulates IL6 signaling. Suppresses fetal liver erythropoiesis. Regulates onset and maintenance of allergic responses mediated by T-helper type 2 cells. Probable substrate recognition component of a SCF-like ECS (Elongin BC-CUL2/5-SOCS-box protein) E3 ubiquitin-protein ligase complex which mediates the ubiquitination and subsequent proteasomal degradation of target proteins[1].

  • Expression


    Tissue_specificity:It is widely expressed, with high expression levels in the heart, placenta, skeletal muscle, peripheral blood leukocytes, human lungs, and fetal liver and kidneys. Expression levels are lower in the thymus.

  • Subunit

    Interacts with multiple activated proteins of the tyrosine kinase signaling pathway including IGF1 receptor, insulin receptor and JAK2. Binding to JAK2 is mediated through the KIR and SH2 domains to a phosphorylated tyrosine residue within the JAK2 JH1 domain (PubMed:10421843). Binds specific activated tyrosine residues of the leptin, EPO, IL12, GSCF and gp130 receptors (PubMed:12027890, PubMed:14559241). Interaction with CSNK1E stabilizes SOCS3 protein (PubMed:15070676). Component of the probable ECS(SOCS3) E3 ubiquitin-protein ligase complex which contains CUL5, RNF7/RBX2, Elongin BC complex and SOCS3 (PubMed:15601820). Interacts with CUL5, RNF7, ELOB and ELOC (PubMed:15601820). Interacts with CUL2 (PubMed:15601820). Interacts with FGFR3 (PubMed:16410555). Interacts with INSR (By similarity). Interacts with BCL10; this interaction may interfere with BCL10-binding with PELI2 (By similarity). Interacts with NOD2 (via CARD domain); the interaction promotes NOD2 degradation (PubMed:23019338)

  • SwissProt ID

    O14543

  • Gene ID
    9021 [NCBI]
  • Synonyms

    CIS3; SSI3; ATOD4; Cish3; SSI-3; SOCS-3

SOCS-3 Antibody (YA3498) Related Classifications

MOQ
Minimum order quantity
100 mg

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Measured by its ability to inhibit anti-CD3-induced proliferation of stimulated CTLL-2 mouse cytotoxic T cells. The ED50 this effect is 0.8023 μg/ml, corresponding to a specific activity is 1246.42 units/mg.

Assay Types
  • 1/16

    MedChemExpress Validation
     Western blot analysis of extracts from PANC-1(lane 1(40μg) ) using SOCS3 antibody. Proteins were transferred to a NC membrane and blocked with 5% Skim milk in TBST for 2 hour at room temperature. The primary antibody ( 1/1000) and Loading control antibody (GAPDH， 1/1000) was used in 5% Skim milk in TBST at 4°C overnight. Goat Anti-Mouse IgG-HRP Secondary Antibody (1/10,000) was used for 1 hour at room temperature.

  • 1/16

    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded human bronchus tissue using SOCS-3 Antibody (HY-P83801, 1/1000). The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • 1/16

    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded human testis tissue using SOCS-3 Antibody (HY-P83801, 1/1000). The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • 1/16

    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded human epididymis tissue using SOCS-3 Antibody (HY-P83801, 1/1000). The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • 1/16

    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded human salivary gland tissue using SOCS-3 Antibody (HY-P83801, 1/1000). The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • 1/16

    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded human kidney tissue using SOCS-3 Antibody (HY-P83801, 1/1000). The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • 1/16

    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded human duodenum tissue using SOCS-3 Antibody (HY-P83801, 1/1000). The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • 1/16

    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded human seminal vesicle tissue using SOCS-3 Antibody (HY-P83801, 1/1000). The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • 1/16

    MedChemExpress Validation
     Immunofluorescence analysis of A549 cells labeling SOCS3 antibody at 1/50 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature.Cells were then incubated with SOCS3 antibody at 1/50 dilution in 1% BSA in PBST overnight at 4 ℃. AF 488 Goat Anti-mouse IgG H&L (green) was used as the secondary antibody at 1/500 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue)

  • SOCS-3 Antibody (YA3498) Product Image
    1/16
  • Experimental Validation Results for SOCS-3 Antibody (YA3498)
  • Experimental Validation Results for SOCS-3 Antibody (YA3498)
  • Experimental Validation Results for SOCS-3 Antibody (YA3498)
  • Experimental Validation Results for SOCS-3 Antibody (YA3498)
  • Experimental Validation Results for SOCS-3 Antibody (YA3498)
  • Experimental Validation Results for SOCS-3 Antibody (YA3498)
  • Experimental Validation Results for SOCS-3 Antibody (YA3498)
  • Experimental Validation Results for SOCS-3 Antibody (YA3498)
  • Experimental Validation Results for SOCS-3 Antibody (YA3498)
  • SOCS-3 Antibody (YA3498) Product Image
  • Experimental Validation Results for SOCS-3 Antibody (YA3498)
    1/1
    MedChemExpress Validation
     Western blot analysis of extracts from PANC-1(lane 1(40μg) ) using SOCS3 antibody. Proteins were transferred to a NC membrane and blocked with 5% Skim milk in TBST for 2 hour at room temperature. The primary antibody ( 1/1000) and Loading control antibody (GAPDH， 1/1000) was used in 5% Skim milk in TBST at 4°C overnight. Goat Anti-Mouse IgG-HRP Secondary Antibody (1/10,000) was used for 1 hour at room temperature.
  • Experimental Validation Results for SOCS-3 Antibody (YA3498)
    1/1
    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded human bronchus tissue using SOCS-3 Antibody (HY-P83801, 1/1000). The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
  • Experimental Validation Results for SOCS-3 Antibody (YA3498)
    1/1
    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded human testis tissue using SOCS-3 Antibody (HY-P83801, 1/1000). The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
  • Experimental Validation Results for SOCS-3 Antibody (YA3498)
    1/1
    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded human epididymis tissue using SOCS-3 Antibody (HY-P83801, 1/1000). The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
  • Experimental Validation Results for SOCS-3 Antibody (YA3498)
    1/1
    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded human salivary gland tissue using SOCS-3 Antibody (HY-P83801, 1/1000). The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
  • Experimental Validation Results for SOCS-3 Antibody (YA3498)
    1/1
    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded human kidney tissue using SOCS-3 Antibody (HY-P83801, 1/1000). The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
  • Experimental Validation Results for SOCS-3 Antibody (YA3498)
    1/1
    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded human duodenum tissue using SOCS-3 Antibody (HY-P83801, 1/1000). The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
  • Experimental Validation Results for SOCS-3 Antibody (YA3498)
    1/1
    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded human seminal vesicle tissue using SOCS-3 Antibody (HY-P83801, 1/1000). The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
  • Experimental Validation Results for SOCS-3 Antibody (YA3498)
    1/1
    MedChemExpress Validation
     Immunofluorescence analysis of A549 cells labeling SOCS3 antibody at 1/50 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature.Cells were then incubated with SOCS3 antibody at 1/50 dilution in 1% BSA in PBST overnight at 4 ℃. AF 488 Goat Anti-mouse IgG H&L (green) was used as the secondary antibody at 1/500 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue)
  • Experimental Validation Results for SOCS-3 Antibody (YA3498)
  • Experimental Validation Results for SOCS-3 Antibody (YA3498)
  • Experimental Validation Results for SOCS-3 Antibody (YA3498)
  • Experimental Validation Results for SOCS-3 Antibody (YA3498)
  • Experimental Validation Results for SOCS-3 Antibody (YA3498)
  • Experimental Validation Results for SOCS-3 Antibody (YA3498)
  • Experimental Validation Results for SOCS-3 Antibody (YA3498)
  • Experimental Validation Results for SOCS-3 Antibody (YA3498)
  • Experimental Validation Results for SOCS-3 Antibody (YA3498)
  • SOCS-3 Antibody (YA3498) Product Image
    MedChemExpress Validation

    Western blot analysis of extracts from PANC-1(lane 1(40μg) ) using SOCS3 antibody. Proteins were transferred to a NC membrane and blocked with 5% Skim milk in TBST for 2 hour at room temperature. The primary antibody ( 1/1000) and Loading control antibody (GAPDH， 1/1000) was used in 5% Skim milk in TBST at 4°C overnight. Goat Anti-Mouse IgG-HRP Secondary Antibody (1/10,000) was used for 1 hour at room temperature.

  • SOCS-3 Antibody (YA3498) Product Image
    MedChemExpress Validation

    Immunohistochemical analysis of paraffin-embedded human bronchus tissue using SOCS-3 Antibody (HY-P83801, 1/1000). The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • SOCS-3 Antibody (YA3498) Product Image
    MedChemExpress Validation

    Immunohistochemical analysis of paraffin-embedded human testis tissue using SOCS-3 Antibody (HY-P83801, 1/1000). The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • SOCS-3 Antibody (YA3498) Product Image
    MedChemExpress Validation

    Immunohistochemical analysis of paraffin-embedded human epididymis tissue using SOCS-3 Antibody (HY-P83801, 1/1000). The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • SOCS-3 Antibody (YA3498) Product Image
    MedChemExpress Validation

    Immunohistochemical analysis of paraffin-embedded human salivary gland tissue using SOCS-3 Antibody (HY-P83801, 1/1000). The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • SOCS-3 Antibody (YA3498) Product Image
    MedChemExpress Validation

    Immunohistochemical analysis of paraffin-embedded human kidney tissue using SOCS-3 Antibody (HY-P83801, 1/1000). The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • SOCS-3 Antibody (YA3498) Product Image
    MedChemExpress Validation

    Immunohistochemical analysis of paraffin-embedded human duodenum tissue using SOCS-3 Antibody (HY-P83801, 1/1000). The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • SOCS-3 Antibody (YA3498) Product Image
    MedChemExpress Validation

    Immunohistochemical analysis of paraffin-embedded human seminal vesicle tissue using SOCS-3 Antibody (HY-P83801, 1/1000). The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • SOCS-3 Antibody (YA3498) Product Image
    MedChemExpress Validation

    Immunofluorescence analysis of A549 cells labeling SOCS3 antibody at 1/50 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature.Cells were then incubated with SOCS3 antibody at 1/50 dilution in 1% BSA in PBST overnight at 4 ℃. AF 488 Goat Anti-mouse IgG H&L (green) was used as the secondary antibody at 1/500 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue)

  • SOCS-3 Antibody (YA3498) Product Image WB
  • SOCS-3 Antibody (YA3498) Product Image IHC-P
  • SOCS-3 Antibody (YA3498) Product Image IHC-P
  • SOCS-3 Antibody (YA3498) Product Image IHC-P
  • SOCS-3 Antibody (YA3498) Product Image IHC-P
  • SOCS-3 Antibody (YA3498) Product Image IHC-P
  • SOCS-3 Antibody (YA3498) Product Image IHC-P
  • SOCS-3 Antibody (YA3498) Product Image IHC-P
  • SOCS-3 Antibody (YA3498) Product Image ICC