PEF1 Antibody (YA9769)
(Synonyms: PEF1, Penta-EF-Hand Domain Containing 1, Peflin, PEF1A, PEF Protein With A Long N-Terminal Hydrophobic Domain, ABP32, Epididymis Secretory Sperm Binding Protein, Penta-EF Hand Domain-Containing Protein 1)PEF1 Antibody (YA9769) is a Rabbit-derived and non-conjugated IgG Monoclonal, Recombinant antibody, targeting to PEF1.
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Host:
Rabbit
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Isotype:
IgG
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Application:
WB, FC, ICC/IF, IHC-P
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Reactivity :
Human, mouse, rat
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Formulation:
Supplied in PBS (pH 7.4) containing 50% glycerol, 0.05% BSA and 0.02% sodium azide.
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Conjugation:
Non-conjugated
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
Applications
| Application |
WB
WB: Western Blot
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FC
FC: Flow Cytometry
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ICC/IF
ICC/IF: Immunocytochemistry/
Immunofluorescence |
IHC-P
IHC-P: Immunohistochemistry-Paraffin
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|---|---|---|---|---|
| Dilution Ratio | 1:1000-5000 | 1:200-2000 | 1:100-1000 | 1:100-200 |
Product Details
PEF1 Antibody (YA9769) is a Rabbit-derived and non-conjugated IgG Monoclonal, Recombinant antibody, targeting to PEF1.
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Host Rabbit
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Species ReactivityHuman, mouse, rat
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Observed Molecular WeightObserved band size: 30 kDa
Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.
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Calculated Molecular Weight Predicted band size: 30 kDa,
A synthesized peptide derived from human PEF1
affinity purified.
Non-conjugated
IgG
Product Properties
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Appearance
Solution
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Formulation
Supplied in PBS (pH 7.4) containing 50% glycerol, 0.05% BSA and 0.02% sodium azide.
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Storage & Stability
Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.
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Shipping
Shipping with blue ice.
Background
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Function
PEF1 is a Calcium-binding protein that acts as an adapter that bridges unrelated proteins or stabilizes weak protein-protein complexes in response to calcium. Together with PDCD6, acts as a calcium-dependent adapter for the BCR(KLHL12) complex, a complex involved in endoplasmic reticulum (ER)-Golgi transport by regulating the size of COPII coats. In response to cytosolic calcium increase, the heterodimer formed with PDCD6 interacts with, and bridges together the BCR(KLHL12) complex and SEC31 (SEC31A or SEC31B), promoting monoubiquitination of SEC31 and subsequent collagen export, which is required for neural crest specification. Its role in the heterodimer formed with PDCD6 is however unclear: some evidence shows that PEF1 and PDCD6 work together and promote association between PDCD6 and SEC31 in presence of calcium. Other reports show that PEF1 dissociates from PDCD6 in presence of calcium, and may act as a negative regulator of PDCD6. Also acts as a negative regulator of ER-Golgi transport; possibly by inhibiting interaction between PDCD6 and SEC31 (By similarity)[1][2].
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Subcellular Localization
Cytoplasm; Endoplasmic reticulum; Membrane; Cytoplasmic vesicle, COPII-coated vesicle membrane
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Isoforms & Post-Translational Modification
PEF1 has an amino acid length of 284, molecular weight is 30381 Da.
Ubiquitinated by the BCR(KLHL12) E3 ubiquitin ligase complex. -
Subunit
Heterodimer; heterodimerizes (via the EF-hand 5) with PDCD6.
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SwissProt ID
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Synonyms
PEF1, Penta-EF-Hand Domain Containing 1, Peflin, PEF1A, PEF Protein With A Long N-Terminal Hydrophobic Domain, ABP32, Epididymis Secretory Sperm Binding Protein, Penta-EF Hand Domain-Containing Protein 1
Documentation
References
[1]. McGourty CA, et al. Regulation of the CUL3 Ubiquitin Ligase by a Calcium-Dependent Co-adaptor. Cell. 2016 Oct 6;167(2):525-538.e14. [Content Brief]
[2]. Kitaura Y, et al. Peflin and ALG-2, members of the penta-EF-hand protein family, form a heterodimer that dissociates in a Ca2+-dependent manner. J Biol Chem. 2001 Apr 27;276(17):14053-8. [Content Brief]
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