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RACK1 Antibody (YA1804)

(Synonyms: H12.3; HLC-7; PIG21; RACK1; Gnb2-rs1)

RACK1 Antibody (YA1804) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to RACK1.

For research use only. We do not sell to patients.
  • Host:

    Rabbit

  • Isotype:

    IgG

  • Application:

    WB, IHC-P

  • Reactivity :

    Human, Mouse, Rat

  • Formulation:

    Supplied in 50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40% Glycerol, 0.01% Sodium azide and 0.05% BSA

  • Conjugation:
    Non-conjugated
  • Experimental Validation Results for RACK1 Antibody (YA1804)
  • Experimental Validation Results for RACK1 Antibody (YA1804)
  • Experimental Validation Results for RACK1 Antibody (YA1804)
  • RACK1 Antibody (YA1804) Product Image
ALL WB IHC-P
  • Experimental Validation Results for RACK1 Antibody (YA1804)
  • Experimental Validation Results for RACK1 Antibody (YA1804)
  • Experimental Validation Results for RACK1 Antibody (YA1804)
  • RACK1 Antibody (YA1804) Product Image
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To place orders, for customer services and technical support, please contact: MedChemExpress USA

Tel: 609-228-6898 E-mail: [email protected] [email protected]

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Applications

Application
WB Info
WB: Western Blot
IHC-P Info
IHC-P: Immunohistochemistry-Paraffin
Dilution Ratio 1:500-1:1000 1:50-1:100

Product Details

Description

RACK1 Antibody (YA1804) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to RACK1.

  • Host Rabbit
  • Clonality Recombinant, Monoclonal
  • Species Reactivity
    Human, Mouse, Rat
  • Observed Molecular Weight
    Observed band size: 35 kDa Info
    Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.
  • Calculated Molecular Weight Predicted band size: 35 kDa
Species Reactivity Database
Immunogen

A synthetic peptide of human RACK1

Sensitivity

Endogenous

Purification

Affinity Purified

Conjugation

Non-conjugated

Modification

Unmodified

Isotype

IgG

RRID

AB_3104179

Product Properties

  • Appearance

    Liquid

  • Formulation

    Supplied in 50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40% Glycerol, 0.01% Sodium azide and 0.05% BSA

  • Storage & Stability

    Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

  • Shipping

    Shipping with blue ice.

Verification Images

  • Experimental Validation Results for RACK1 Antibody (YA1804)
    Western blot analysis of extracts from C6 (lane 2(20μg), NIH3T3 (lane 3(20μg) and Hela (lane 4(20μg) using RACK1 (HY-P82059) Rabbit mAb. Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P80438, 1/10000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Mouse IgG-HRP Secondary Antibody (1/10000) was used for 1 hour at room temperature.
  • Experimental Validation Results for RACK1 Antibody (YA1804)
    Immunohistochemical analysis of paraffin-embedded Mouse liver tissue using RACK1 Antibody (YA1804). The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 8 minutes. The tissues were blocked in QuickBlock for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HY-P82059, 1/100) in 4℃ overnight. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
  • Experimental Validation Results for RACK1 Antibody (YA1804)
    Immunohistochemical analysis of paraffin-embedded Mouse liver tissue using RACK1 Antibody (YA1804). The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 8 minutes. The tissues were blocked in QuickBlock for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HY-P82059, 1/100) in 4℃ overnight. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Background

  • Function

    Scaffolding protein involved in the recruitment, assembly and/or regulation of a variety of signaling molecules. Interacts with a wide variety of proteins and plays a role in many cellular processes. Component of the 40S ribosomal subunit involved in translational repression (PubMed:23636399). Involved in the initiation of the ribosome quality control (RQC), a pathway that takes place when a ribosome has stalled during translation, by promoting ubiquitination of a subset of 40S ribosomal subunits (PubMed:28132843). Binds to and stabilizes activated protein kinase C (PKC), increasing PKC-mediated phosphorylation. May recruit activated PKC to the ribosome, leading to phosphorylation of EIF6. Inhibits the activity of SRC kinases including SRC, LCK and YES1. Inhibits cell growth by prolonging the G0/G1 phase of the cell cycle. Enhances phosphorylation of BMAL1 by PRKCA and inhibits transcriptional activity of the BMAL1-CLOCK heterodimer. Facilitates ligand-independent nuclear translocation of AR following PKC activation, represses AR transactivation activity and is required for phosphorylation of AR by SRC. Modulates IGF1R-dependent integrin signaling and promotes cell spreading and contact with the extracellular matrix. Involved in PKC-dependent translocation of ADAM12 to the cell membrane. Promotes the ubiquitination and proteasome-mediated degradation of proteins such as CLEC1B and HIF1A. Required for VANGL2 membrane localization, inhibits Wnt signaling, and regulates cellular polarization and oriented cell division during gastrulation. Required for PTK2/FAK1 phosphorylation and dephosphorylation. Regulates internalization of the muscarinic receptor CHRM2. Promotes apoptosis by increasing oligomerization of BAX and disrupting the interaction of BAX with the anti-apoptotic factor BCL2L. Inhibits TRPM6 channel activity. Regulates cell surface expression of some GPCRs such as TBXA2R. Plays a role in regulation of FLT1-mediated cell migration. Involved in the transport of ABCB4 from the Golgi to the apical bile canalicular membrane (PubMed:19674157). Promotes migration of breast carcinoma cells by binding to and activating RHOA (PubMed:20499158). Acts as an adapter for the dephosphorylation and inactivation of AKT1 by promoting recruitment of PP2A phosphatase to AKT1 (By similarity); (Microbial infection) Binds to Y.pseudotuberculosis yopK which leads to inhibition of phagocytosis and survival of bacteria following infection of host cells; (Microbial infection) Enhances phosphorylation of HIV-1 Nef by PKCs; (Microbial infection) In case of poxvirus infection, remodels the ribosomes so that they become optimal for the viral mRNAs (containing poly-A leaders) translation but not for host mRNAs; (Microbial infection) Contributes to the cap-independent internal ribosome entry site (IRES)-mediated translation by some RNA viruses

  • Subcellular Localization

    Cell membrane; Peripheral membrane protein; Cytoplasm; Cytoplasm, perinuclear region; Nucleus; Perikaryon; Cell projection, dendrite; Cell projection, phagocytic cup

  • Expression


    Tissue_specificity:In the liver, expressed at higher levels in activated hepatic stellate cells than in hepatocytes or Kupffer cells. Up-regulated in hepatocellular carcinomas and in the adjacent non-tumor liver tissue

  • Subunit

    Monomer; also forms homodimers and homooligomers (PubMed:15140893, PubMed:20529362). Interacts with CPNE3 (PubMed:20010870). May interact with ABCB4 (PubMed:19674157).

  • SwissProt ID

    P63244

  • Gene ID
    10399 [NCBI]
  • Synonyms

    H12.3; HLC-7; PIG21; RACK1; Gnb2-rs1

  • Research Field

    Cell Biology

RACK1 Antibody (YA1804) Related Classifications

Measured by its ability to inhibit anti-CD3-induced proliferation of stimulated CTLL-2 mouse cytotoxic T cells. The ED50 this effect is 0.8023 μg/ml, corresponding to a specific activity is 1246.42 units/mg.

Assay Types
  • 1/16

    RACK1 Antibody (YA1804) purchased from MedChemExpress. Usage Cited in:

    MedchemExpress Validation
     Western blot analysis of extracts from C6 (lane 2(20μg), NIH3T3 (lane 3(20μg) and Hela (lane 4(20μg) using RACK1 (HY-P82059) Rabbit mAb. Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P80438, 1/10000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Mouse IgG-HRP Secondary Antibody (1/10000) was used for 1 hour at room temperature.

  • 1/16

    RACK1 Antibody (YA1804) purchased from MedChemExpress. Usage Cited in:

    MedchemExpress Validation
     Immunohistochemical analysis of paraffin-embedded Mouse liver tissue using RACK1 Antibody (YA1804). The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 8 minutes. The tissues were blocked in QuickBlock for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HY-P82059, 1/100) in 4℃ overnight. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • 1/16

    RACK1 Antibody (YA1804) purchased from MedChemExpress. Usage Cited in:

    MedchemExpress Validation
     Immunohistochemical analysis of paraffin-embedded Mouse liver tissue using RACK1 Antibody (YA1804). The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 8 minutes. The tissues were blocked in QuickBlock for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HY-P82059, 1/100) in 4℃ overnight. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • RACK1 Antibody (YA1804) Product Image
    1/16
  • Experimental Validation Results for RACK1 Antibody (YA1804)
  • Experimental Validation Results for RACK1 Antibody (YA1804)
  • Experimental Validation Results for RACK1 Antibody (YA1804)
  • RACK1 Antibody (YA1804) Product Image
  • RACK1 Antibody (YA1804) Product Image
    MedchemExpress Validation

    Western blot analysis of extracts from C6 (lane 2(20μg), NIH3T3 (lane 3(20μg) and Hela (lane 4(20μg) using RACK1 (HY-P82059) Rabbit mAb. Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P80438, 1/10000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Mouse IgG-HRP Secondary Antibody (1/10000) was used for 1 hour at room temperature.

  • RACK1 Antibody (YA1804) Product Image
    MedchemExpress Validation

    Immunohistochemical analysis of paraffin-embedded Mouse liver tissue using RACK1 Antibody (YA1804). The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 8 minutes. The tissues were blocked in QuickBlock for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HY-P82059, 1/100) in 4℃ overnight. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • RACK1 Antibody (YA1804) Product Image
    MedchemExpress Validation

    Immunohistochemical analysis of paraffin-embedded Mouse liver tissue using RACK1 Antibody (YA1804). The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 8 minutes. The tissues were blocked in QuickBlock for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HY-P82059, 1/100) in 4℃ overnight. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • RACK1 Antibody (YA1804) Product Image WB
  • RACK1 Antibody (YA1804) Product Image IHC-P
  • RACK1 Antibody (YA1804) Product Image IHC-P