RACK1 - receptor for activated C kinase 1 Gene

Also Known as H12.3; HLC-7; PIG21; GNB2L1; Gnb2-rs1

Species: Homo sapiens

Gene Type: protein coding

Gene ID: 10399

About RACK1

Cytogenetic location: 5q35.3 Genomic coordinates (GRCh38): 5:181,236,897-181,243,906 (from NCBI)

This gene has 35 transcripts (splice variants) and 222 orthologues. Ubiquitous expression in ovary (RPKM 1262.3), lymph node (RPKM 598.8) and 25 other tissues.

Summary

Enables several functions, including cyclin binding activity; enzyme binding activity; and protein domain specific binding activity. Involved in several processes, including positive regulation of hydrolase activity; regulation of cellular protein metabolic process; and regulation of signal transduction. Located in several cellular components, including midbody; perinuclear region of cytoplasm; and phagocytic cup. Part of IRE1-RACK1-PP2A complex. [provided by Alliance of Genome Resources, Apr 2022]

RACK1 Products (1)

mRNA	Protein	Name
NM_006098.5	NP_006089.1	receptor of activated protein C kinase 1

Gene Ontology

Molecular Function
Biological Process
Cellular Component

Molecular Function GO Annotation	Evidence	References	Source
enables BH3 domain binding	IDA IDA: Inferred from direct assay	20541605	GOA
enables SH2 domain binding	IDA IDA: Inferred from direct assay	9584165	GOA
enables cyclin binding	IPI IPI: Inferred from physical interaction	15159402	GOA
enables cysteine-type endopeptidase activator activity involved in apoptotic process	IMP IMP: Inferred from mutant phenotype	19767770	GOA
enables enzyme activator activity	IDA IDA: Inferred from direct assay	20819076	GOA
enables enzyme binding	IPI IPI: Inferred from physical interaction	20819076	GOA
enables identical protein binding	IPI IPI: Inferred from physical interaction	24947010	GOA
enables protein binding	IPI IPI: Inferred from physical interaction	10849009	GOA
enables protein homodimerization activity	IPI IPI: Inferred from physical interaction	24947010	GOA
enables protein kinase C binding	IDA IDA: Inferred from direct assay	11279199	GOA
enables protein phosphatase binding	IPI IPI: Inferred from physical interaction	11278757	GOA
enables protein tyrosine kinase inhibitor activity	IDA IDA: Inferred from direct assay	9584165	GOA
enables receptor tyrosine kinase binding	IDA IDA: Inferred from direct assay	9584165	GOA
enables ribosome binding	IDA IDA: Inferred from direct assay	28132843	GOA
enables signaling adaptor activity	IMP IMP: Inferred from mutant phenotype	20103773	GOA

Biological Process GO Annotation	Evidence	References	Source
involved in activation of cysteine-type endopeptidase activity involved in apoptotic process	IMP IMP: Inferred from mutant phenotype	19767770	GOA
involved in cellular response to glucose stimulus	IDA IDA: Inferred from direct assay	20103773	GOA
involved in cellular response to growth factor stimulus	IDA IDA: Inferred from direct assay	20010870	GOA
involved in negative regulation of cell growth	IDA IDA: Inferred from direct assay	9584165	GOA
involved in negative regulation of gene expression	IMP IMP: Inferred from mutant phenotype	19451233	GOA
involved in negative regulation of intrinsic apoptotic signaling pathway in response to hydrogen peroxide	IGI IGI: Inferred from genetic interaction	24947010	GOA
involved in negative regulation of peptidyl-serine phosphorylation	IMP IMP: Inferred from mutant phenotype	20103773	GOA
involved in negative regulation of phagocytosis	IMP IMP: Inferred from mutant phenotype	21347310	GOA
involved in negative regulation of phosphatidylinositol 3-kinase/protein kinase B signal transduction	IMP IMP: Inferred from mutant phenotype	19767770	GOA
involved in negative regulation of protein binding	IDA IDA: Inferred from direct assay	20541605	GOA
involved in negative regulation of smoothened signaling pathway	IDA IDA: Inferred from direct assay	32467643	GOA
involved in positive regulation of GTPase activity	IDA IDA: Inferred from direct assay	20499158	GOA
involved in positive regulation of Golgi to plasma membrane protein transport	IMP IMP: Inferred from mutant phenotype	19674157	GOA
involved in positive regulation of apoptotic process	IDA IDA: Inferred from direct assay	20541605	GOA
involved in positive regulation of apoptotic process	IMP IMP: Inferred from mutant phenotype	19767770	GOA
involved in positive regulation of cell migration	IDA IDA: Inferred from direct assay	20499158	GOA
involved in positive regulation of intrinsic apoptotic signaling pathway	IMP IMP: Inferred from mutant phenotype	19767770	GOA
involved in positive regulation of mitochondrial depolarization	IMP IMP: Inferred from mutant phenotype	19767770	GOA
involved in positive regulation of proteasomal ubiquitin-dependent protein catabolic process	IDA IDA: Inferred from direct assay	19785988	GOA
involved in positive regulation of protein phosphorylation	IDA IDA: Inferred from direct assay	11312657	GOA
involved in positive regulation of protein-containing complex assembly	IDA IDA: Inferred from direct assay	20541605	GOA
involved in protein ubiquitination	IMP IMP: Inferred from mutant phenotype	28132843	GOA
involved in regulation of adenylate cyclase-activating G protein-coupled receptor signaling pathway	IMP IMP: Inferred from mutant phenotype	20819076	GOA
involved in regulation of cell cycle	IDA IDA: Inferred from direct assay	9584165	GOA
involved in rescue of stalled ribosome	IMP IMP: Inferred from mutant phenotype	28132843	GOA

Cellular Component GO Annotation	Evidence	References	Source
part of IRE1-RACK1-PP2A complex	IDA IDA: Inferred from direct assay	20103773	GOA
located in cytoplasm	IDA IDA: Inferred from direct assay	10849009	GOA
located in cytosol	IMP IMP: Inferred from mutant phenotype	19767770	GOA
located in midbody	IDA IDA: Inferred from direct assay	15166316	GOA
located in mitochondrion	IMP IMP: Inferred from mutant phenotype	19767770	GOA
located in nucleus	IDA IDA: Inferred from direct assay	10849009	GOA
located in perinuclear region of cytoplasm	IDA IDA: Inferred from direct assay	11279199	GOA
located in phagocytic cup	IDA IDA: Inferred from direct assay	21347310	GOA

EXP: Inferred from Experiment IDA: Inferred from direct assay IPI: Inferred from physical interaction IMP: Inferred from mutant phenotype IGI: Inferred from genetic interaction IEP: Inferred from expression pattern

RACK1 Protein Structure

WD40

0
100
200
300
317 a.a.

Protein Preferred Names	Protein Names
receptor of activated protein C kinase 1	cell proliferation-inducing gene 21 protein

RACK1 Protein-protein interaction Information

Type	Protein Name	Protein ID	Interactor	Interactor Species	Interactor ID	Detection Method	References
Intra	RACK1	P63244	GGN	Homo sapiens	Q86UU5	Y2H Prey Pooling	32296183
Intra	RACK1	P63244	GGN	Homo sapiens	Q86UU5	Validated Y2H	32296183
Intra	RACK1	P63244	GGN	Homo sapiens	Q86UU5	Y2H Array	32296183
Intra	RACK1	P63244	LARP4B	Homo sapiens	Q92615	Y2H Prey Pooling	32296183
Intra	RACK1	P63244	LARP4B	Homo sapiens	Q92615	Anti Tag CoIP	28514442
Intra	RACK1	P63244	LARP4B	Homo sapiens	Q92615	Validated Y2H	32296183
Intra	RACK1	P63244	LARP4B	Homo sapiens	Q92615	Y2H Array	32296183
Intra	RACK1	P63244	LARP4B	Homo sapiens	Q92615	Anti Tag CoIP	35271311
Intra	RACK1	P63244	LARP4B	Homo sapiens	Q92615	Y2H	21988832
Intra	RACK1	P63244	USP54	Homo sapiens	Q70EL1-9	Validated Y2H	32296183
Intra	RACK1	P63244	USP54	Homo sapiens	Q70EL1-9	Y2H Array	32296183
Intra	RACK1	P63244	USP54	Homo sapiens	Q70EL1-9	Y2H Prey Pooling	32296183
Intra	RACK1	P63244	ZSCAN12	Homo sapiens	O43309	Y2H Prey Pooling	32296183
Intra	RACK1	P63244	ZSCAN12	Homo sapiens	O43309	Y2H Array	32296183
Intra	RACK1	P63244	HELZ	Homo sapiens	P42694	Anti Tag CoIP	35271311
Intra	RACK1	P63244	HELZ	Homo sapiens	P42694	Anti Tag CoIP	33961781
Intra	RACK1	P63244	HELZ	Homo sapiens	P42694	Anti Tag CoIP	28514442
Intra	RACK1	P63244	TMEM131	Homo sapiens	Q0P631	Y2H Array	32296183
Intra	RACK1	P63244	TMEM131	Homo sapiens	Q0P631	Y2H Prey Pooling	32296183
Intra	RACK1	P63244	TMEM131	Homo sapiens	Q0P631	Validated Y2H	32296183
Intra	RACK1	P63244	MKRN2	Homo sapiens	Q9H000	Y2H Prey Pooling	32296183
Intra	RACK1	P63244	MKRN2	Homo sapiens	Q9H000	Validated Y2H	32296183
Intra	RACK1	P63244	MKRN2	Homo sapiens	Q9H000	Y2H Array	32296183
Intra	RACK1	P63244	GPBP1	Homo sapiens	Q86WP2	Y2H Array	32296183
Intra	RACK1	P63244	GPBP1	Homo sapiens	Q86WP2	Validated Y2H	32296183
Intra	RACK1	P63244	GPBP1	Homo sapiens	Q86WP2	Y2H Prey Pooling	32296183
Intra	RACK1	P63244	USP10	Homo sapiens	Q14694	Y2H Prey Pooling	32296183
Intra	RACK1	P63244	USP10	Homo sapiens	Q14694	Validated Y2H	32296183
Intra	RACK1	P63244	USP10	Homo sapiens	Q14694	Y2H Array	32296183
Intra	RACK1	P63244	USP10	Homo sapiens	Q14694	Anti Tag CoIP	35271311
Intra	RACK1	P63244	PAG1	Homo sapiens	Q9NWQ8	Anti Bait CoIP	23866081
Intra	RACK1	P63244	ARRDC3	Homo sapiens	Q96B67	Y2H Prey Pooling	32296183
Intra	RACK1	P63244	ARRDC3	Homo sapiens	Q96B67	Validated Y2H	32296183
Intra	RACK1	P63244	ARRDC3	Homo sapiens	Q96B67	Y2H Array	32296183
Intra	RACK1	P63244	LRP12	Homo sapiens	Q9Y561	Y2H	12809483
Intra	RACK1	P63244	RPL7	Homo sapiens	P18124	Density Sedimentation	21376236
Intra	RACK1	P63244	RPL26	Homo sapiens	P61254	Density Sedimentation	21376236
Intra	RACK1	P63244	RPL7	Homo sapiens	P18124	3D-EM	29143818
Intra	RACK1	P63244	RPL7	Homo sapiens	P18124	Anti Tag CoIP	35271311
Intra	RACK1	P63244	RPS3	Homo sapiens	P23396	Anti Bait CoIP	21525958
Intra	RACK1	P63244	RPS3	Homo sapiens	P23396	Anti Tag CoIP	35271311
Intra	RACK1	P63244	RPL7	Homo sapiens	P18124	3D-EM	25901680
Intra	RACK1	P63244	RPL26	Homo sapiens	P61254	3D-EM	25901680
Intra	RACK1	P63244	RPS3	Homo sapiens	P23396	3D-EM	29143818
Intra	RACK1	P63244	RPL26	Homo sapiens	P61254	3D-EM	29143818
Intra	RACK1	P63244	RPS3	Homo sapiens	P23396	3D-EM-Single	32908316
Intra	RACK1	P63244	RPL26	Homo sapiens	P61254	Anti Tag CoIP	35271311
Intra	RACK1	P63244	RPS3	Homo sapiens	P23396	3D-EM	25901680
Intra	RACK1	P63244	VHL	Homo sapiens	P40337	Anti Tag CoIP	20871634
Intra	RACK1	P63244	VHL	Homo sapiens	P40337	Confocal	20871634
Intra	RACK1	P63244	PIAS2	Homo sapiens	O75928	Anti Bait CoIP	22210188
Intra	RACK1	P63244	DYNLL1	Homo sapiens	P63167	Pull Down	18420585
Intra	RACK1	P63244	DYNLL1	Homo sapiens	P63167	Anti Tag CoIP	18420585
Intra	RACK1	P63244	BYSL	Homo sapiens	Q13895	Validated Y2H	32296183
Intra	RACK1	P63244	BYSL	Homo sapiens	Q13895	Anti Tag CoIP	35271311
Intra	RACK1	P63244	CRMP1	Homo sapiens	Q14194	Validated Y2H	32296183
Intra	RACK1	P63244	CRMP1	Homo sapiens	Q14194	Y2H Array	32296183
Intra	RACK1	P63244	CRMP1	Homo sapiens	Q14194	Y2H Prey Pooling	32296183
Cross	RACK1	P63244	Bcl2l11	Mus musculus	O54918-1	Anti Tag CoIP	18420585
Intra	RACK1	P63244	BCL2L11	Homo sapiens	O43521	Anti Bait CoIP	18420585
Intra	RACK1	P63244	AGO2	Homo sapiens	Q9UKV8	Anti Bait CoIP	21525958
Intra	RACK1	P63244	PPID	Homo sapiens	Q08752	Anti Tag CoIP	21146485
Intra	RACK1	P63244	CNOT2	Homo sapiens	Q9NZN8	Y2H Prey Pooling	32296183
Intra	RACK1	P63244	CNOT2	Homo sapiens	Q9NZN8	Validated Y2H	32296183
Intra	RACK1	P63244	CNOT2	Homo sapiens	Q9NZN8	Y2H Array	32296183
Intra	RACK1	P63244	LYN	Homo sapiens	P07948	Anti Bait CoIP	23866081
Intra	RACK1	P63244	EED	Homo sapiens	O75530	Anti Bait CoIP	20080539
Intra	RACK1	P63244	IFNAR2	Homo sapiens	P48551	Anti Bait CoIP	11046044
Cross	RACK1	P63244	bzlf1_ebvb9	Epstein-Barr virus	P03206	Pull Down	10849009
Cross	RACK1	P63244	bzlf1_ebvb9	Epstein-Barr virus	P03206	Anti Bait CoIP	10849009
Cross	RACK1	P63244	bzlf1_ebvb9	Epstein-Barr virus	P03206	Y2H	10849009

Cross: Cross-species interaction Intra: Intraspecies interaction

Recombinant RACK1 Proteins

Cat. No.	Product Name	Accession	Purity
HY-P74606	RACK1 Protein, Human (His-MBP)	P63244 (M1-R317)	≥ 95%, as determined by reducing SDS-PAGE.

RACK1 Antibodies

Cat. No.	Product Name	Application	Reactivity
HY-P82059	RACK1 Antibody (YA1804)	WB, IHC-P	Human, Mouse, Rat
HY-P82059A	RACK1 Antibody (YA1804)(PBS only)	WB, IHC-P	Human, Mouse, Rat

Related Diseases

Diseases	Alias
Melanomatosis
Lung Cancer	Lung Carcinoma Non-Small Cell Lung Carcinoma Lung Cancer, Susceptibility To Lung Cancer, Protection Against Adenocarcinoma Of Lung, Somatic Adenocarcinoma Of Lung, Response To Tyrosine Kinase Inhibitor In Nonsmall Cell Lung Cancer Lung Neoplasm Carcinoma Of Lung Lung Non-Small Cell Carcinoma Non-Small Cell Lung Cancer Nsclc Lung Neoplasms Malignant Neoplasm Of Lung Alveolar Cell Carcinoma Nonsmall Cell Lung Cancer, Somatic Nonsmall Cell Lung Cancer, Response To Tyrosine Kinase Inhibitor In Nonsmall Cell Lung Cancer, Susceptibility To Lung Cancer, Somatic Lung Cancer, Resistance To Cancer Of Lung Cancer Of Bronchus Cancer Of The Lung Lung Malignancies Lung Malignant Tumors Malignant Lung Tumor Malignant Tumor Of Lung Pulmonary Cancer Pulmonary Carcinoma Pulmonary Neoplasms Respiratory Carcinoma LNCR Adenocarcinoma Of Lung Neoplasm Of Lung Cancer Lung Carcinoma Non-Small Cell Lung Carcinoma, Non-Small-Cell Lung Lung Cancers Lung Carcinomas Cancer, Lung Cancer, Lung, Non-Small Cell Primary Malignant Neoplasm Of Lung Bronchioloalveolar Adenocarcinoma

Diseases	Alias
Waldenstroem'S Macroglobulinemia	Waldenstroem'S Macroglobulinemia Waldenstroem'S Macroglobulinemia Macroglobulinemia Of Waldenstrom Lymphoplasmacytic Lymphoma With Igm Gammopathy Lymphoplasmacytic Lymphoma Waldenstroem'S Macroglobulinemia Waldenstroem'S Macroglobulinemia Macroglobulinemia Of Waldenstrom Lymphoplasmacytic Lymphoma With Igm Gammopathy Lymphoplasmacytic Lymphoma

Related Agents

Pre-clinical Phase

Pre-clinical Phase

Bioactive Molecules (11)

Cat. No.	Product Name	Effect	Purity	Rare Diseases
HY-P0221C	PACAP (1-38) free acid		99.94%	Unkown
HY-141891	M435-1279	Inhibitor	98.43%	Unkown
HY-N10335	Harringtonolide	Inhibitor	98.0%	Unkown
HY-162254	EGFR/ACK1-IN-1	Inhibitor	98.19%	Unkown
HY-RS11595	RACK1 Human Pre-designed siRNA Set A	Pre-designed Set	/	Unkown
HY-P0221B	PACAP (1-38) free acid TFA		/	Unkown
HY-P1371	Pseudo RACK1	Agonist	/	Unkown
HY-P3879	Protein Kinase C (660-673)		/	Unkown
HY-181165	SB2960	Inhibitor	/	Unkown
HY-RS16781	Rack1 Mouse Pre-designed siRNA Set A	Pre-designed Set	/	Unkown
HY-RS23221	Rack1 Rat Pre-designed siRNA Set A	Pre-designed Set	/	Unkown

Orthologs Information

Species	Symbol	Source	ID
Felis catus	RACK1	VGNC	VGNC:102300
Bos taurus	RACK1	VGNC	VGNC:33676
Rattus norvegicus	RACK1	RGD	RGD:69229
Canis familiaris	RACK1	VGNC	VGNC:45309
Mus musculus	RACK1	MGD	MGI:101849
Macaca mulatta	RACK1	VGNC	VGNC:76581
Others	RACK1	NCBI