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  3. RACK1 Protein, Human (His-MBP)

RACK1 Protein, Human (His-MBP)

Cat. No.: HY-P74606
COA Handling Instructions

The RACK1 protein is a multifunctional scaffold protein that participates in multiple cellular processes by recruiting, assembling, and regulating signaling molecules. As part of the 40S ribosomal subunit, it contributes to translation repression and initiates the ribosome quality control (RQC) pathway by promoting ubiquitination of specific 40S ribosomal subunits. RACK1 Protein, Human (His-MBP) is the recombinant human-derived RACK1 protein, expressed by E. coli , with N-His, N-MBP labeled tag. The total length of RACK1 Protein, Human (His-MBP) is 317 a.a., with molecular weight of ~70 kDa.

For research use only. We do not sell to patients.

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Description

The RACK1 protein is a multifunctional scaffold protein that participates in multiple cellular processes by recruiting, assembling, and regulating signaling molecules. As part of the 40S ribosomal subunit, it contributes to translation repression and initiates the ribosome quality control (RQC) pathway by promoting ubiquitination of specific 40S ribosomal subunits. RACK1 Protein, Human (His-MBP) is the recombinant human-derived RACK1 protein, expressed by E. coli , with N-His, N-MBP labeled tag. The total length of RACK1 Protein, Human (His-MBP) is 317 a.a., with molecular weight of ~70 kDa.

Background

RACK1, functioning as a versatile scaffolding protein, intricately participates in diverse cellular processes by recruiting, assembling, and regulating various signaling molecules. As a component of the 40S ribosomal subunit, it contributes to translational repression and is pivotal in initiating the ribosome quality control (RQC) pathway, particularly by promoting the ubiquitination of a subset of 40S ribosomal subunits. RACK1 binds and stabilizes activated protein kinase C (PKC), thereby enhancing PKC-mediated phosphorylation and potentially recruiting it to the ribosome for the phosphorylation of EIF6. Furthermore, RACK1 exerts inhibitory effects on SRC kinases, including SRC, LCK, and YES1, leading to cell cycle arrest at the G0/G1 phase. Its regulatory role extends to diverse cellular functions, such as modulating integrin signaling, promoting apoptosis, regulating membrane localization of various receptors, and influencing cell migration. Additionally, RACK1 is implicated in pathogen-host interactions, as evidenced by its binding to Yersinia pseudotuberculosis yopK, resulting in the inhibition of phagocytosis and facilitating bacterial survival within host cells.

Species

Human

Source

E. coli

Tag

N-His;N-MBP

Accession

P63244 (M1-R317)

Gene ID
Molecular Construction
N-term
His-MBP
RACK1 (M1-R317)
Accession # P63244
C-term
Synonyms
Receptor of activated protein C kinase 1; HLC-7; RACK1; GNB2L1
AA Sequence

MTEQMTLRGTLKGHNGWVTQIATTPQFPDMILSASRDKTIIMWKLTRDETNYGIPQRALRGHSHFVSDVVISSDGQFALSGSWDGTLRLWDLTTGTTTRRFVGHTKDVLSVAFSSDNRQIVSGSRDKTIKLWNTLGVCKYTVQDESHSEWVSCVRFSPNSSNPIIVSCGWDKLVKVWNLANCKLKTNHIGHTGYLNTVTVSPDGSLCASGGKDGQAMLWDLNEGKHLYTLDGGDIINALCFSPNRYWLCAATGPSIKIWDLEGKIIVDELKQEVISTSSKAEPPQCTSLAWSADGQTLFAGYTDNLVRVWQVTIGTR

Molecular Weight

Approximately 70 kDa

Purity
  • Greater than 95% as determined by reducing SDS-PAGE.
Appearance

Lyophilized powder

Formulation

Lyophilized from a 0.2 μm filtered solution of PBS, pH 7.5. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween 80 are added as protectants before lyophilization.

Endotoxin Level

<1 EU/μg, determined by LAL method.

Reconstitution

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH2O.For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Storage & Stability

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Shipping

Room temperature in continental US; may vary elsewhere.

Documentation

RACK1 Protein, Human (His-MBP) Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

  • Reconstitution Calculator

  • Dilution Calculator

  • Specific Activity Calculator

The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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Product Name:
RACK1 Protein, Human (His-MBP)
Cat. No.:
HY-P74606
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