RACK1 Protein, Human (His-MBP)

RACK1, functioning as a versatile scaffolding protein, intricately participates in diverse cellular processes by recruiting, assembling, and regulating various signaling molecules. As a component of the 40S ribosomal subunit, it contributes to translational repression and is pivotal in initiating the ribosome quality control (RQC) pathway, particularly by promoting the ubiquitination of a subset of 40S ribosomal subunits. RACK1 binds and stabilizes activated protein kinase C (PKC), thereby enhancing PKC-mediated phosphorylation and potentially recruiting it to the ribosome for the phosphorylation of EIF6. Furthermore, RACK1 exerts inhibitory effects on SRC kinases, including SRC, LCK, and YES1, leading to cell cycle arrest at the G0/G1 phase. Its regulatory role extends to diverse cellular functions, such as modulating integrin signaling, promoting apoptosis, regulating membrane localization of various receptors, and influencing cell migration. Additionally, RACK1 is implicated in pathogen-host interactions, as evidenced by its binding to Yersinia pseudotuberculosis yopK, resulting in the inhibition of phagocytosis and facilitating bacterial survival within host cells.