IL-6R Antibody(PBS only)
(Synonyms: CD126; gp80; IL-6R-1; IL-6RA; IL6Q; IL6RA; IL6RQ)IL-6R Antibody is a mouse-derived and non-conjugated IgG1 monoclonal antibody, targeting to IL-6R.
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Host:
Mouse
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Isotype:
IgG
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Application:
WB, IHC-P, ICC/IF, FC, ELISA
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Reactivity :
Human, Mouse
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Formulation:
Supplied in PBS, pH 7.4.
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Conjugation:
Non-conjugated
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
Applications
| Application |
WB
WB: Western Blot
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IHC-P
IHC-P: Immunohistochemistry-Paraffin
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ICC/IF
ICC/IF: Immunocytochemistry/
Immunofluorescence |
FC
FC: Flow Cytometry
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ELISA
ELISA: Enzyme Linked Immunosorbent Assay
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|---|---|---|---|---|---|
| Dilution Ratio | 1:500-1:1000 | 1:100-1:200 | 1:50-1:200 | 1:50-1:100 | 1:10000 |
Product Details
IL-6R Antibody is a mouse-derived and non-conjugated IgG1 monoclonal antibody, targeting to IL-6R.
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Host Mouse
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Species ReactivityHuman, Mouse
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Observed Molecular WeightObserved band size: 52 kDa
Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.
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Calculated Molecular Weight Predicted band size: 52 kDa
Purified recombinant fragment of human CD126 expressed in E. Coli.
Affinity Purified
Non-conjugated
IgG
Product Properties
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Appearance
Solution
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Formulation
Supplied in PBS, pH 7.4.
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Storage & Stability
Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.
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Shipping
Shipping with blue ice.
Verification Images
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Western blot analysis of extracts from Jurkat(lane 2(20ug) , THP-1(lane 3(20ug) and HEK293(lane 4(20ug)using IL-6R Antibody (HY-P81629) Rabbit mAb. Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P83730, 1/10000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Mouse IgG-HRP Secondary Antibody (1/10000) was used for 1 hour at room temperature. -
Immunohistochemical analysis of paraffin-embedded mouse spleen tissue using IL-6R Antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked in QuickBlock for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody at 1/100 dilution in 4℃ overnight. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. -
Immunohistochemical analysis of paraffin-embedded mouse spleen tissue using IL-6R Antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked in QuickBlock for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody at 1/100 dilution in 4℃ overnight. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. -
Immunocytochemistry analysis of Hela cells labeling IL-6R with IL-6R Antibody (HY-P81629) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 for 10 minutes at room temperature, then blocked with QuickBlock™ Blocking Buffer for Immunol Staining for 10 min at room temperature. Cells were then incubated with IL-6R Antibody ((HY-P81629) at 1/100 dilution in QuickBlock™ Blocking Buffer for Immunol Staining at 4 ℃. Alexa Fluor® 488-conjugated AffiniPure Goat Anti-Mouse IgG H&L（Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue). -
Immunocytochemistry analysis of Hela cells labeling IL-6R with IL-6R Antibody (HY-P81629) at 1/50 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 for 10 minutes at room temperature, then blocked with QuickBlock™ Blocking Buffer for Immunol Staining for 10 min at room temperature. Cells were then incubated with IL-6R Antibody ((HY-P81629) at 1/50 dilution in QuickBlock™ Blocking Buffer for Immunol Staining at 4 ℃. Alexa Fluor® 488-conjugated AffiniPure Goat Anti-Mouse IgG H&L（Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue). -
Flow cytometric analysis of 1.5X10^6 U937 cells labeling IL-6R Antibody (HY-P81629, red). Cells were fixed with 4% paraformaldehyde. Then stained with the primary antibody at 1/200 dilution for an hour at 4℃. Goat Anti-Rabbit IgG H&L (AF488) (HY-P8002) was used as the secondary antibody at 1/1,000 dilution for 30 minutes at 4℃. Rabbit IgG Isotype Control (HY-P80879, blue) was used as the isotype control, cells without incubation with primary antibody were used as the unlabeled control (black).
Background
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Function
Interleukin-6 receptor (IL-6R) is a receptor for IL-6, belonging to the type I cytokine receptor family, subfamily 3. IL-6 is a potent pleiotropic cytokine that regulates cell growth and differentiation and plays an important role in immune response. IL-6R is a protein complex composed of this protein and the interleukin-6 signal transductor (IL6ST/GP130/IL6β) . This receptor subunit is also shared by many other cytokines. Dysproduction of IL-6 and this receptor has been implicated in the pathogenesis of many diseases, such as multiple myeloma, autoimmune diseases and prostate cancer. In addition, IL-6Rα (IL-6RA) is the primary functional alpha subunit of the IL-6 receptor, which is also a component of other interleukin receptors. IL-6RA is a type I transmembrane glycoprotein that regulates the biological activity of IL-6 by forming a complex with CD130[1]. There are two isoforms of IL-6Rα, including mIL6R (long form) or sIL6R (short form) . The sIL-6R membrane binds IL6R and subunit IL6ST, which activate regenerative and anti-inflammatory signals through IL-6 transduction signals and promote the pro-inflammatory properties of IL-6. Therefore, the hydrolysis of IL-6R is also known as exdomain shedding[2]. IL-6Rα is an IL-6 agonist and also involved in JAK/STAT, MAPK and Akt signaling pathways[2][3]. IL-6R also plays an important role in acute response and hematopoietic response[4]. However, the signal transduction of IL6 is limited, because IL-6RA is tissue specific and only expressed specifically in some cells of liver and immune system[5].
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Subcellular Localization
Cell membrane; Single-pass type I membrane protein; Secreted; Secreted
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Expression
Tissue_specificity:sIL6R is mainly expressed in peripheral blood mononuclear cells, with low levels in urine and serum. 1%-20% of total sIL6R in plasma is generated by alternative splicing (PubMed:28060820) . -
Isoforms & Post-Translational Modification
P08887 has 2 isomers: P08887-1: 51548 Da (predicted); P08887-2: 40237 Da (predicted).
A short soluble form is released from the membrane by proteolysis (PubMed:26876177). The sIL6R is formed mostly by limited proteolysis of membrane-bound receptors, a process referred to as ectodomain shedding, but is also directly secreted from the cells after alternative mRNA splicing (PubMed:26876177, PubMed:28060820). mIL6R is cleaved by the proteases ADAM10 and ADAM17 (PubMed:26876177, PubMed:28060820);Glycosylated. Glycosylation is dispensable for transport, signaling, and cell-surface turnover. Glycosylation at Asn-55 is a protease-regulatory exosite. Glycosylation is required for ADAM17-mediated proteolysis -
Subunit
Component of a hexamer of two molecules each of IL6, IL6R and IL6ST; first binds to IL6 to associate with the signaling subunit IL6ST (PubMed:12829785, PubMed:28265003). Interacts (via N-terminal ectodomain) with SORL1; this interaction may affect IL6-binding to IL6R, hence decrease IL6 'classic-signaling' (PubMed:28265003)
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SwissProt ID
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Synonyms
CD126; gp80; IL-6R-1; IL-6RA; IL6Q; IL6RA; IL6RQ
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Research Field
Immunology
Documentation
References
[1]. Garbers C, et al. Inhibition of classic signaling is a novel function of soluble glycoprotein 130 (sgp130), which is controlled by the ratio of interleukin 6 and soluble interleukin 6 receptor. J Biol Chem. 2011 Dec 16;286(50):42959-70. [Content Brief]
[3]. Larsen JV, et al. SorLA in Interleukin-6 Signaling and Turnover. Mol Cell Biol. 2017 May 16;37(11):e00641-16. [Content Brief]
[4]. Giannitrapani L, et al. Circulating IL-6 and sIL-6R in patients with hepatocellular carcinoma. Ann N Y Acad Sci. 2002 Jun;963:46-52. [Content Brief]
[5]. Arnold P, et al. Joint Reconstituted Signaling of the IL-6 Receptor via Extracellular Vesicles. Cells. 2020 May 24;9(5):1307. [Content Brief]
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