CDK16 Antibody (YA515)(PBS only)

Cat. No.: HY-P80612A: Ficha de datos User Guide for Antibodies
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CDK16 Antibody (YA515) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to CDK16.

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Tamaño		Stock
50 μL		Obtener un presupuesto
100 μL		Obtener un presupuesto

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WB: Western Blot;
IHC-P: Immunohistochemistry-Paraffin;
IHC-F: Immunohistochemistry-Frozen;
ICC/IF: Immunocytochemistry/Immunofluorescence;
IF-Tissue: Immunofluorescence-Tissue;
mIHC: Multiplex Immunohistochemical;
IP: Immunoprecipitation;
ChIP: Chromatin Immunoprecipitation;
FC: Flow Cytometry;
ELISA: Enzyme Linked Immunosorbent Assay

Product Detail
Background
Descripciòn

Descripciòn

CDK16 Antibody (YA515) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to CDK16.

Host

Rabbit

Peso molecular

Predicted band size: 56 kDa;

Observed band size: 65-70 kDa

Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.

Species Reactivity

Human

SwissProt ID

Q00536

Gene ID

5127 [NCBI]

Immunogen

Synthetic peptide corresponding to Human PCTAIRE1.The exact sequence is proprietary to MCE.

Application &
Dilution Ratio

Application	Dilution Ratio
WB WB: Western Blot	1:500-1:1000

Pureza	affinity purified	Conjugation	Non-conjugated
Isotype	IgG

Appearance

Liquid

Formulation

Supplied in PBS, pH 7.4.

Storage & Stability

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

Envío

Shipping with blue ice.

Background

Function：Protein kinase that plays a role in vesicle-mediated transport processes and exocytosis. Regulates GH1 release by brain neurons. Phosphorylates NSF, and thereby regulates NSF oligomerization. Required for normal spermatogenesis. Regulates neuron differentiation and dendrite development (By similarity). Plays a role in the regulation of insulin secretion in response to changes in blood glucose levels. Can phosphorylate CCNY at 'Ser-336' (in vitro)

Subcellular Localization：Cytoplasm; Cytoplasmic vesicle, secretory vesicle; Cell membrane; Peripheral membrane protein; Cytoplasmic side; Synapse, synaptosome

Expression：
Tissue_specificity:Detected in pancreas islets (at protein level) . Detected in brain and pancreas

Isoforms & Post-Translational Modification：Q00536 has 3 isomers: Q00536-1: 55716 Da (predicted); Q00536-2: 63458 Da (predicted); Q00536-3: 56375 Da (predicted).
Phosphorylation of CDK16 is essential for the binding of CCNY, but also essential for the regulation of CDK16 kinase activity (PubMed:22798068). Phosphorylation of CDK16 is essential for the binding of CCNYl1, but also essential for the regulation of CDK16 kinase activity (By similarity). Ser-146 and Ser-153 are the most critical sites for the binding of CCNYL1 and for modulating CDK16 kinase activity (By similarity). Phosphorylation at Ser-153 inhibits kinase activity (PubMed:22798068)

Subunit：Found in a complex containing CABLES1, CDK17 and TDRD7. Interacts with BRSK2. Identified in a complex with NSF, syntaxin-1, synaptotagmin, SYN1, SYP and CDK5R1 (By similarity). Interacts with YWHAH, YWHAQ and YWHAZ. Interacts with CCNY; this interaction increases the CDK16 kinase activity (By similarity). Interacts with CCNYL1; this interaction mutually increases the stability of CDK16 and CCNYL1 and increases the kinase activity of CDK16 (By similarity). Interacts with NSF (By similarity)

Research Field

Signal Transduction

Synonyms

PCTK1; PCTAIRE; PCTAIRE1; PCTGAIRE

Documentación

User Guide for Antibodies (1077 KB)