PPP1R15A (GADD34) Antibody (YA7791)(PBS only)

Cat. No.: HY-P88107A: Data Sheet User Guide for Antibodies
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PPP1R15A (GADD34) Antibody (YA7791) is a Mouse-derived and non-conjugated IgG2b monoclonal antibody, targeting to PPP1R15A (GADD34).

For research use only. We do not sell to patients.

Size		Stock
50 μL		Get quote
100 μL		Get quote

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WB: Western Blot;
IHC-P: Immunohistochemistry-Paraffin;
IHC-F: Immunohistochemistry-Frozen;
ICC/IF: Immunocytochemistry/Immunofluorescence;
IF-Tissue: Immunofluorescence-Tissue;
mIHC: Multiplex Immunohistochemical;
IP: Immunoprecipitation;
ChIP: Chromatin Immunoprecipitation;
FC: Flow Cytometry;
ELISA: Enzyme Linked Immunosorbent Assay

Product Detail
Background
Documentation

Description

PPP1R15A (GADD34) Antibody (YA7791) is a Mouse-derived and non-conjugated IgG2b monoclonal antibody, targeting to PPP1R15A (GADD34).

Host

Mouse

Molecular Weight

Predicted band size: 73.3 kDa

Species Reactivity

Human, Dog, Rat, Monkey, Mouse

SwissProt ID

O75807

Immunogen

Full length human recombinant protein of human PPP1R15A produced in HEK293T cell.

Application &
Dilution Ratio

Application	Dilution Ratio
WB WB: Western Blot	1:500~2000
IHC-P IHC-P: Immunohistochemistry-Paraffin	1:150
ICC/IF ICC/IF: Immunocytochemistry/Immunofluorescence	1:100
FC FC: Flow Cytometry	1:100

Purity	Affinity purified	Conjugation	Non-conjugated
Isotype	IgG2b

Appearance

Liquid

Formulation

Supplied in PBS, pH 7.4.

Storage & Stability

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

Shipping

Shipping with blue ice.

Background

Function：Recruits the serine/threonine-protein phosphatase PPP1CA to prevents excessive phosphorylation of the translation initiation factor eIF-2A/EIF2S1, thereby reversing the shut-off of protein synthesis initiated by stress-inducible kinases and facilitating recovery of cells from stress (PubMed:26095357, PubMed:26742780). Down-regulates the TGF-beta signaling pathway by promoting dephosphorylation of TGFB1 by PP1 (PubMed:14718519). May promote apoptosis by inducing p53/TP53 phosphorylation on 'Ser-15' (PubMed:14635196). Plays an essential role in autophagy by tuning translation during starvation, thus enabling lysosomal biogenesis and a sustained autophagic flux (PubMed:32978159). Also acts a viral restriction factor by attenuating HIV-1 replication (PubMed:31778897). Mechanistically, mediates the inhibition of HIV-1 TAR RNA-mediated translation (PubMed:31778897)|(Microbial infection) Promotes enterovirus 71 replication by mediating the internal ribosome entry site (IRES) activity of viral 5'-UTR

Subcellular Localization：Endoplasmic reticulum membrane,Mitochondrion outer membrane

Expression：Induction: Specifically produced in response to stress: in absence of stress, some upstream open reading frame (uORF) of this transcript is translated, thereby preventing its translation (PubMed:19131336). By methyl methanesulfonate and ionizing irradiation (PubMed:9153226). By IL24/interleukin-24 in melanoma cells; which induces apoptosis (PubMed:10490642, PubMed:12114539). By viral infection including enterovirus 71/EV71 or HIV-1 (PubMed:31778897, PubMed:34985336)

Isoforms & Post-Translational Modification：O75807 has two isomers: O75807-1: 73478 Da (predicted); O75807-2: 56515 Da (predicted).
Phosphorylated at multiple Ser/Thr residues丨Polyubiquitinated

Subunit：Interacts with PPP1CA (PubMed:15705855, PubMed:26095357)

Synonyms

GADD34

Documentation

User Guide for Antibodies (1077 KB)