PPP1R15A (GADD34) Antibody (YA7791)(PBS only)
(Synonyms: GADD34)
Based on 1 Customer Validation
PPP1R15A (GADD34) Antibody (YA7791) is a Mouse-derived and non-conjugated IgG2b monoclonal antibody, targeting to PPP1R15A (GADD34).
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Host:
Mouse
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Isotype:
IgG1
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Application:
WB, IHC-P, ICC/IF, FC
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Reactivity :
Human
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Formulation:
Supplied in PBS, pH 7.4.
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Conjugation:
Non-conjugated
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
Applications
| Application |
WB
WB: Western Blot
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IHC-P
IHC-P: Immunohistochemistry-Paraffin
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ICC/IF
ICC/IF: Immunocytochemistry/
Immunofluorescence |
FC
FC: Flow Cytometry
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|---|---|---|---|---|
| Dilution Ratio | 1:500-2000 | 1:150 | 1:100 | 1:100 |
Product Details
PPP1R15A (GADD34) Antibody (YA7791) is a Mouse-derived and non-conjugated IgG2b monoclonal antibody, targeting to PPP1R15A (GADD34).
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Host Mouse
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Species ReactivityHuman
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Calculated Molecular Weight Predicted band size: 73.3 kDa
Full length human recombinant protein of human PPP1R15A produced in HEK293T cell.
Affinity purified
Non-conjugated
IgG1
Product Properties
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Appearance
Solution
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Formulation
Supplied in PBS, pH 7.4.
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Concentration
Batch-dependent, Please check the COA for the concentration of each lot. Check Lot Concentration
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Storage & Stability
Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.
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Shipping
Shipping with blue ice.
Background
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Function
PPP1R15A recruits the serine/threonine-protein phosphatase PPP1CA to prevents excessive phosphorylation of the translation initiation factor eIF-2A/EIF2S1, thereby reversing the shut-off of protein synthesis initiated by stress-inducible kinases and facilitating recovery of cells from stress. Down-regulates the TGF-beta signaling pathway by promoting dephosphorylation of TGFB1 by PP1. May promote apoptosis by inducing p53/TP53 phosphorylation on 'Ser-15'. Plays an essential role in autophagy by tuning translation during starvation, thus enabling lysosomal biogenesis and a sustained autophagic flux. Also acts a viral restriction factor by attenuating HIV-1 replication. Mechanistically, mediates the inhibition of HIV-1 TAR RNA-mediated translation |(Microbial infection) Promotes enterovirus 71 replication by mediating the internal ribosome entry site (IRES) activity of viral 5'-UTR[1][2][3][4][5][6].
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Subcellular Localization
Endoplasmic reticulum membrane,Mitochondrion outer membrane
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Expression
Induction: Specifically produced in response to stress: in absence of stress, some upstream open reading frame (uORF) of this transcript is translated, thereby preventing its translation (PubMed:19131336). By methyl methanesulfonate and ionizing irradiation (PubMed:9153226). By IL24/interleukin-24 in melanoma cells; which induces apoptosis (PubMed:10490642, PubMed:12114539). By viral infection including enterovirus 71/EV71 or HIV-1 (PubMed:31778897, PubMed:34985336)
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Isoforms & Post-Translational Modification
O75807 has two isomers: O75807-1: 73478 Da (predicted); O75807-2: 56515 Da (predicted).
Phosphorylated at multiple Ser/Thr residues丨Polyubiquitinated -
Subunit
Interacts with PPP1CA (PubMed:15705855, PubMed:26095357)
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SwissProt ID
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Synonyms
GADD34
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