Copy Link Print Information

UBE2M Antibody (YA7797)

(Synonyms: hUbc12; UBC-RS2; UBC12)
Customer Review

Based on 1 Customer Validation

UBE2M Antibody (YA7797) is a Mouse-derived and non-conjugated IgG1 monoclonal antibody, targeting to UBE2M.

For research use only. We do not sell to patients.
  • Host:

    Mouse

  • Isotype:

    IgG1

  • Application:

    WB, ICC/IF

  • Reactivity :

    Human, Dog, Monkey, Mouse, Rat

  • Formulation:

    Spplied in PBS (pH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide.

  • Conjugation:
    Non-conjugated
  • Experimental Validation Results for UBE2M Antibody (YA7797)
  • UBE2M Antibody (YA7797) Product Image
Size
Price
Stock
Quantity
Amount: USD 0.00
Please select quantity
or
or

* Please select Quantity before adding items.

To place orders, for customer services and technical support, please contact: MedChemExpress USA

Tel: 609-228-6898 E-mail: [email protected] [email protected]

Service
Data Download
Related Products:
Top Publications Citing

Use of MCE Products

Applications

Application
WB Info
WB: Western Blot
ICC/IF Info
ICC/IF: Immunocytochemistry/
Immunofluorescence
Dilution Ratio 1:1000-2000 1:100-250

Product Details

Description

UBE2M Antibody (YA7797) is a Mouse-derived and non-conjugated IgG1 monoclonal antibody, targeting to UBE2M.

  • Host Mouse
  • Clonality Monoclonal
  • Species Reactivity
    Human, Dog, Monkey, Mouse, Rat
  • Calculated Molecular Weight Predicted band size: 20.7 kDa
Immunogen

Full length human recombinant protein of human UBE2M produced in HEK293T cell.

Sensitivity

Endogenous

Purification

Affinity purified

Conjugation

Non-conjugated

Modification

Unmodified

Isotype

IgG1

Product Properties

  • Appearance

    Solution

  • Formulation

    Spplied in PBS (pH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide.

  • Concentration

    Batch-dependent, Please check the COA for the concentration of each lot. Check Lot Concentration

  • Storage & Stability

    Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

  • Shipping

    Shipping with blue ice.

Verification Images

  • Experimental Validation Results for UBE2M Antibody (YA7797)
    Western blot analysis was performed on protein extracts (25 μg) from HeLa (lane 2), 293T (lane 3), Ramos (lane 4), MCF-7 (lane 5), and HT-1080 (lane 6) using UBE2M antibody. Proteins were transferred onto a 0.45 μm PVDF membrane using the Trans-Blot® Turbo™ system for 13 min. The membrane was then blocked with 5% nonfat milk in TBST (HY-K1025) for 1 h at room temperature. The primary antibody (1:1000) and loading control antibody GAPDH Antibody (HRP) (HY-P80954A) (1:5000) were diluted in 5% nonfat milk in TBST and incubated with the membrane overnight at 4°C. After washing, the membrane of primary antibody was incubated with HRP-conjugated goat anti-rabbit/mouse IgG secondary antibody (HY-P8001/HY-P8004) (1:5000) diluted in 5% nonfat milk in TBST for 1 h at room temperature. Protein bands were visualized using an Ultra High Sensitivity ECL detection kit (HY-K1005).

Background

  • Function

    UBE2M is an Accepts the ubiquitin-like protein NEDD8 from the UBA3-NAE1 E1 complex and catalyzes its covalent attachment to other proteins. The specific interaction with the E3 ubiquitin ligase RBX1, but not RBX2, suggests that the RBX1-UBE2M complex neddylates specific target proteins, such as CUL1, CUL2, CUL3 and CUL4. Involved in cell proliferation

  • Isoforms & Post-Translational Modification

    P61081 has two isomers: P61081-1: 20900 Da (predicted); P61081-2: 24373 Da (predicted).
    The acetylation of Met-1 increases affinity for DCUN1D1 by about 2 orders of magnitude and is crucial for NEDD8 transfer to cullins

  • Subunit

    Interacts with UBA3 and RBX1

  • SwissProt ID

    P61081

  • Synonyms

    hUbc12; UBC-RS2; UBC12

UBE2M Antibody (YA7797) Related Classifications

MOQ
Minimum order quantity
100 mg

Get Quote In-stock

Other size
Get Quote
Please select quantity
Amount: USD 0.00

Measured by its ability to inhibit anti-CD3-induced proliferation of stimulated CTLL-2 mouse cytotoxic T cells. The ED50 this effect is 0.8023 μg/ml, corresponding to a specific activity is 1246.42 units/mg.

Assay Types
  • 1/16

    MedChemExpress Validation
     Western blot analysis was performed on protein extracts (25 μg) from HeLa (lane 2), 293T (lane 3), Ramos (lane 4), MCF-7 (lane 5), and HT-1080 (lane 6) using UBE2M antibody. Proteins were transferred onto a 0.45 μm PVDF membrane using the Trans-Blot® Turbo™ system for 13 min. The membrane was then blocked with 5% nonfat milk in TBST (HY-K1025) for 1 h at room temperature. The primary antibody (1:1000) and loading control antibody GAPDH Antibody (HRP) (HY-P80954A) (1:5000) were diluted in 5% nonfat milk in TBST and incubated with the membrane overnight at 4°C. After washing, the membrane of primary antibody was incubated with HRP-conjugated goat anti-rabbit/mouse IgG secondary antibody (HY-P8001/HY-P8004) (1:5000) diluted in 5% nonfat milk in TBST for 1 h at room temperature. Protein bands were visualized using an Ultra High Sensitivity ECL detection kit (HY-K1005).

  • UBE2M Antibody (YA7797) Product Image
    1/16
  • Experimental Validation Results for UBE2M Antibody (YA7797)
  • UBE2M Antibody (YA7797) Product Image
  • Experimental Validation Results for UBE2M Antibody (YA7797)
    1/1
    MedChemExpress Validation
     Western blot analysis was performed on protein extracts (25 μg) from HeLa (lane 2), 293T (lane 3), Ramos (lane 4), MCF-7 (lane 5), and HT-1080 (lane 6) using UBE2M antibody. Proteins were transferred onto a 0.45 μm PVDF membrane using the Trans-Blot® Turbo™ system for 13 min. The membrane was then blocked with 5% nonfat milk in TBST (HY-K1025) for 1 h at room temperature. The primary antibody (1:1000) and loading control antibody GAPDH Antibody (HRP) (HY-P80954A) (1:5000) were diluted in 5% nonfat milk in TBST and incubated with the membrane overnight at 4°C. After washing, the membrane of primary antibody was incubated with HRP-conjugated goat anti-rabbit/mouse IgG secondary antibody (HY-P8001/HY-P8004) (1:5000) diluted in 5% nonfat milk in TBST for 1 h at room temperature. Protein bands were visualized using an Ultra High Sensitivity ECL detection kit (HY-K1005).
  • Experimental Validation Results for UBE2M Antibody (YA7797)
  • UBE2M Antibody (YA7797) Product Image
    MedChemExpress Validation

    Western blot analysis was performed on protein extracts (25 μg) from HeLa (lane 2), 293T (lane 3), Ramos (lane 4), MCF-7 (lane 5), and HT-1080 (lane 6) using UBE2M antibody. Proteins were transferred onto a 0.45 μm PVDF membrane using the Trans-Blot® Turbo™ system for 13 min. The membrane was then blocked with 5% nonfat milk in TBST (HY-K1025) for 1 h at room temperature. The primary antibody (1:1000) and loading control antibody GAPDH Antibody (HRP) (HY-P80954A) (1:5000) were diluted in 5% nonfat milk in TBST and incubated with the membrane overnight at 4°C. After washing, the membrane of primary antibody was incubated with HRP-conjugated goat anti-rabbit/mouse IgG secondary antibody (HY-P8001/HY-P8004) (1:5000) diluted in 5% nonfat milk in TBST for 1 h at room temperature. Protein bands were visualized using an Ultra High Sensitivity ECL detection kit (HY-K1005).

  • UBE2M Antibody (YA7797) Product Image WB