Flotillin-1 Antibody (YA4632)

Cat. No.: HY-P84936: Ficha de datos COA
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Flotillin-1 Antibody (YA4632) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to Flotillin-1.

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10 μL	En stock	Estimated Time of Arrival: December 31
50 μL	En stock	Estimated Time of Arrival: December 31
100 μL	En stock	Estimated Time of Arrival: December 31
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WB: Western Blot;
IHC-P: Immunohistochemistry-Paraffin;
IHC-F: Immunohistochemistry-Frozen;
ICC/IF: Immunocytochemistry/Immunofluorescence;
IF-Tissue: Immunofluorescence-Tissue;
mIHC: Multiplex Immunohistochemical;
IP: Immunoprecipitation;
ChIP: Chromatin Immunoprecipitation;
FC: Flow Cytometry;
ELISA: Enzyme Linked Immunosorbent Assay

Product Detail
Verification Image
Background
Descripciòn
Referencias

Descripciòn

Flotillin-1 Antibody (YA4632) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to Flotillin-1.

Host

Rabbit

Clonality

Monoclonal, Recombinant

Peso molecular

Predicted band size: 47 kDa;

Observed band size: 47 kDa

Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.

Species Reactivity

Human

SwissProt ID

O75955

Gene ID

10211 [NCBI]

Immunogen

A synthetic peptide of human Flotillin 1

Application &
Dilution Ratio

Application	Dilution Ratio
WB WB: Western Blot	1:500-1:1000
ICC/IF ICC/IF: Immunocytochemistry/Immunofluorescence	1:50-1:200
IP IP: Immunoprecipitation	1:20

Pureza	affinity purified	Conjugation	Non-conjugated
Modification	Unmodified	Isotype	IgG

Appearance

Liquid

Formulation

Supplied in 50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40%Glycerol, 0.01% sodium azide and 0.05% BSA.

Storage & Stability

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

Envío

Shipping with blue ice.

Verification Image

Western blot analysis was performed on extracts from Hela (lane 1, 15 μg), Jurkat (lane 2, 15 μg), K562 (lane 3, 15 μg), A431 (lane 4, 15 μg), 293T (lane 5, 15 μg), and 293 (lane 6, 15 μg) using Flotillin 1 Rabbit mAb.Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST at 4°C overnight.The primary antibody (1:1000 dilution) and the loading control antibody (GAPDH, HY-P80137, 1:40000 dilution) were incubated in 5% non-fat milk in TBST for 1 hour at 37°C.Goat Anti-Rabbit IgG-HRP Secondary Antibody (1:20000 dilution) was then applied for 40 minutes at 37°C.

Background

Function：May act as a scaffolding protein within caveolar membranes, functionally participating in formation of caveolae or caveolae-like vesicles

Subcellular Localization：Cell membrane; Peripheral membrane protein; Endosome; Membrane, caveola; Peripheral membrane protein; Melanosome; Membrane raft

Isoforms & Post-Translational Modification：O75955 has 2 isomers: O75955-1: 47355 Da (predicted); O75955-2: 42080 Da (predicted).

Subunit：Heterooligomeric complex of flotillin-1 and flotillin-2 and caveolin-1 and caveolin-2 (By similarity). Interacts with ECPAS

Research Field

Cell Biology

Synonyms

Flotillin-1

Documentación

Select Batch:

Ficha de datos (232 KB) SDS (251 KB)

COA (206 KB)

User Guide for Antibodies (1077 KB)

Referencias

[1]. /biology-dictionary/hela-cell.html [Content Brief]