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Flotillin-1 Antibody (YA4632)

(Synonyms: Flotillin-1)
Customer Review

Based on 1 Customer Validation

Flotillin-1 Antibody (YA4632) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to Flotillin-1.

For research use only. We do not sell to patients.
  • Host:

    Rabbit

  • Isotype:

    IgG

  • Application:

    WB, ICC/IF, IP

  • Reactivity :

    Human

  • Formulation:

    Supplied in 50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40%Glycerol, 0.01% sodium azide and 0.05% BSA.

  • Conjugation:
    Non-conjugated
  • Experimental Validation Results for Flotillin-1 Antibody (YA4632)
  • Flotillin-1 Antibody (YA4632) Product Image
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To place orders, for customer services and technical support, please contact: MedChemExpress USA

Tel: 609-228-6898 E-mail: [email protected] [email protected]

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Related Products:
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Use of MCE Products

Applications

Application
WB Info
WB: Western Blot
ICC/IF Info
ICC/IF: Immunocytochemistry/
Immunofluorescence
IP Info
IP: Immunoprecipitation
Dilution Ratio 1:500-1:1000 1:50-1:200 1:20

Product Details

Description

Flotillin-1 Antibody (YA4632) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to Flotillin-1.

  • Host Rabbit
  • Clonality Monoclonal, Recombinant
  • Species Reactivity
    Human
  • Observed Molecular Weight
    Observed band size: 47 kDa Info
    Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.
  • Calculated Molecular Weight Predicted band size: 47 kDa
Immunogen

A synthetic peptide of human Flotillin 1

Purification

affinity purified

Conjugation

Non-conjugated

Modification

Unmodified

Isotype

IgG

Product Properties

  • Appearance

    Liquid

  • Formulation

    Supplied in 50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40%Glycerol, 0.01% sodium azide and 0.05% BSA.

  • Concentration

    Batch-dependent, Please check the COA for the concentration of each lot. Check Lot Concentration

  • Storage & Stability

    Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

  • Shipping

    Shipping with blue ice.

Verification Images

  • Experimental Validation Results for Flotillin-1 Antibody (YA4632)
    Western blot analysis was performed on extracts from Hela (lane 1, 15 μg), Jurkat (lane 2, 15 μg), K562 (lane 3, 15 μg), A431 (lane 4, 15 μg), 293T (lane 5, 15 μg), and 293 (lane 6, 15 μg) using Flotillin 1 Rabbit mAb.Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST at 4°C overnight.The primary antibody (1:1000 dilution) and the loading control antibody (GAPDH, HY-P80137, 1:40000 dilution) were incubated in 5% non-fat milk in TBST for 1 hour at 37°C.Goat Anti-Rabbit IgG-HRP Secondary Antibody (1:20000 dilution) was then applied for 40 minutes at 37°C.

Background

  • Function

    Flotillin-1 may act as a scaffolding protein within caveolar membranes, functionally participating in formation of caveolae or caveolae-like vesicles

  • Subcellular Localization

    Cell membrane; Peripheral membrane protein; Endosome; Membrane, caveola; Peripheral membrane protein; Melanosome; Membrane raft

  • Isoforms & Post-Translational Modification

    O75955 has 2 isomers: O75955-1: 47355 Da (predicted); O75955-2: 42080 Da (predicted).

  • Subunit

    Heterooligomeric complex of flotillin-1 and flotillin-2 and caveolin-1 and caveolin-2 (By similarity). Interacts with ECPAS

  • SwissProt ID

    O75955

  • Gene ID
    10211 [NCBI]
  • Synonyms

    Flotillin-1

  • Research Field

    Cell Biology

Flotillin-1 Antibody (YA4632) Related Classifications

MOQ
Minimum order quantity
100 mg

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Measured by its ability to inhibit anti-CD3-induced proliferation of stimulated CTLL-2 mouse cytotoxic T cells. The ED50 this effect is 0.8023 μg/ml, corresponding to a specific activity is 1246.42 units/mg.

Assay Types
  • 1/16

    MedChemExpress Validation
     Western blot analysis was performed on extracts from Hela (lane 1, 15 μg), Jurkat (lane 2, 15 μg), K562 (lane 3, 15 μg), A431 (lane 4, 15 μg), 293T (lane 5, 15 μg), and 293 (lane 6, 15 μg) using Flotillin 1 Rabbit mAb.Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST at 4°C overnight.The primary antibody (1:1000 dilution) and the loading control antibody (GAPDH, HY-P80137, 1:40000 dilution) were incubated in 5% non-fat milk in TBST for 1 hour at 37°C.Goat Anti-Rabbit IgG-HRP Secondary Antibody (1:20000 dilution) was then applied for 40 minutes at 37°C.

  • Flotillin-1 Antibody (YA4632) Product Image
    1/16
  • Experimental Validation Results for Flotillin-1 Antibody (YA4632)
  • Flotillin-1 Antibody (YA4632) Product Image
  • Experimental Validation Results for Flotillin-1 Antibody (YA4632)
    1/1
    MedChemExpress Validation
     Western blot analysis was performed on extracts from Hela (lane 1, 15 μg), Jurkat (lane 2, 15 μg), K562 (lane 3, 15 μg), A431 (lane 4, 15 μg), 293T (lane 5, 15 μg), and 293 (lane 6, 15 μg) using Flotillin 1 Rabbit mAb.Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST at 4°C overnight.The primary antibody (1:1000 dilution) and the loading control antibody (GAPDH, HY-P80137, 1:40000 dilution) were incubated in 5% non-fat milk in TBST for 1 hour at 37°C.Goat Anti-Rabbit IgG-HRP Secondary Antibody (1:20000 dilution) was then applied for 40 minutes at 37°C.
  • Experimental Validation Results for Flotillin-1 Antibody (YA4632)
  • Flotillin-1 Antibody (YA4632) Product Image
    MedChemExpress Validation

    Western blot analysis was performed on extracts from Hela (lane 1, 15 μg), Jurkat (lane 2, 15 μg), K562 (lane 3, 15 μg), A431 (lane 4, 15 μg), 293T (lane 5, 15 μg), and 293 (lane 6, 15 μg) using Flotillin 1 Rabbit mAb.Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST at 4°C overnight.The primary antibody (1:1000 dilution) and the loading control antibody (GAPDH, HY-P80137, 1:40000 dilution) were incubated in 5% non-fat milk in TBST for 1 hour at 37°C.Goat Anti-Rabbit IgG-HRP Secondary Antibody (1:20000 dilution) was then applied for 40 minutes at 37°C.

  • Flotillin-1 Antibody (YA4632) Product Image WB