Neuregulin SMDF Antibody
(Synonyms: GGF, HGL, HRGA, NDF, SMDF, NRG1, Pro-NRG1)Neuregulin SMDF Antibody is a Rabbit-derived and non-conjugated IgG Polyclonal antibody, targeting to Neuregulin SMDF.
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Host:
Rabbit
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Isotype:
IgG
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Application:
WB, IHC-P, ICC/IF
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Reactivity :
Human, Mouse, Rat
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Formulation:
Supplied in PBS (pH 7.4), containing 30% glycerol, and 0.01% sodium azide.
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Conjugation:
Non-conjugated
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
Applications
| Application |
WB
WB: Western Blot
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IHC-P
IHC-P: Immunohistochemistry-Paraffin
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ICC/IF
ICC/IF: Immunocytochemistry/
Immunofluorescence |
|---|---|---|---|
| Dilution Ratio | 1:1000-2000 | 1:100-200 | 1:50-200 |
Product Details
Neuregulin SMDF Antibody is a Rabbit-derived and non-conjugated IgG Polyclonal antibody, targeting to Neuregulin SMDF.
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Host Rabbit
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Clonality Polyclonal
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Species ReactivityHuman, Mouse, Rat
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Observed Molecular WeightObserved band size: 32 kDa
Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.
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Calculated Molecular Weight Predicted band size: 31 kDa
Synthetic peptide corresponding to the N-term region of human Neuregulin SMDF.
Endogenous
affinity purified.
Non-conjugated
Unmodified
IgG
Product Properties
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Appearance
Solution
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Formulation
Supplied in PBS (pH 7.4), containing 30% glycerol, and 0.01% sodium azide.
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Storage & Stability
Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.
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Shipping
Shipping with blue ice.
Background
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Function
Neuregulin SMDF is a Direct ligand for ERBB3 and ERBB4 tyrosine kinase receptors. Concomitantly recruits ERBB1 and ERBB2 coreceptors, resulting in ligand-stimulated tyrosine phosphorylation and activation of the ERBB receptors. The multiple isoforms perform diverse functions such as inducing growth and differentiation of epithelial, glial, neuronal, and skeletal muscle cells; inducing expression of acetylcholine receptor in synaptic vesicles during the formation of the neuromuscular junction; stimulating lobuloalveolar budding and milk production in the mammary gland and inducing differentiation of mammary tumor cells; stimulating Schwann cell proliferation; implication in the development of the myocardium such as trabeculation of the developing heart. Isoform 10 may play a role in motor and sensory neuron development. Binds to ERBB4. Binds to ERBB3. Acts as a ligand for integrins and binds (via EGF domain) to integrins ITGAV:ITGB3 or ITGA6:ITGB4. Its binding to integrins and subsequent ternary complex formation with integrins and ERRB3 are essential for NRG1-ERBB signaling. Induces the phosphorylation and activation of MAPK3/ERK1, MAPK1/ERK2 and AKT1. Ligand-dependent ERBB4 endocytosis is essential for the NRG1-mediated activation of these kinases in neurons (By similarity)[1][2][3].
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Subcellular Localization
Cell membrane
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Expression
Tissue_Specificity: Type I isoforms are the predominant forms expressed in the endocardium. Isoform alpha is expressed in breast, ovary, testis, prostate, heart, skeletal muscle, lung, placenta liver, kidney, salivary gland, small intestine and brain, but not in uterus, stomach, pancreas, and spleen. Isoform 3 is the predominant form in mesenchymal cells and in non-neuronal organs, whereas isoform 6 is the major neuronal form. Isoform 8 is expressed in spinal cord and brain. Isoform 9 is the major form in skeletal muscle cells; in the nervous system it is expressed in spinal cord and brain. Also detected in adult heart, placenta, lung, liver, kidney, and pancreas. Isoform 10 is expressed in nervous system: spinal cord motor neurons, dorsal root ganglion neurons, and brain. Predominant isoform expressed in sensory and motor neurons. Not detected in adult heart, placenta, lung, liver, skeletal muscle, kidney, and pancreas. Not expressed in fetal lung, liver and kidney. Type IV isoforms are brain-specific. -
Isoforms & Post-Translational Modification
Neuregulin SMDF has 11 isoforms, Q02297-1: amino acid length is 640, molecular weight is 70392 Da (predicted); Q02297-2: amino acid length is 648, molecular weight is 71331 Da (predicted); Q02297-3: amino acid length is 462, molecular weight is 50879 Da (predicted); Q02297-4: amino acid length is 247, molecular weight is 26590 Da (predicted); Q02297-6: amino acid length is 645, molecular weight is 71157 Da (predicted); Q02297-7: amino acid length is 637, molecular weight is 70200 Da (predicted); Q02297-8: amino acid length is 241, molecular weight is 26143 Da (predicted); Q02297-9: amino acid length is 422, molecular weight is 45141 Da (predicted); Q02297-10: amino acid length is 296, molecular weight is 31686 Da (predicted); Q02297-11: amino acid length is 590, molecular weight is 65460 Da (predicted); Q02297-12: amino acid length is 420, molecular weight is 46226 Da (predicted).
Proteolytic cleavage close to the plasma membrane on the external face leads to the release of the soluble growth factor form. -
Subunit
The cytoplasmic domain interacts with the LIM domain region of LIMK1 (By similarity).
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SwissProt ID
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Synonyms
GGF, HGL, HRGA, NDF, SMDF, NRG1, Pro-NRG1
Documentation
References
[1]. Sweeney C, et al. Ligand discrimination in signaling through an ErbB4 receptor homodimer. J Biol Chem. 2000 Jun 30;275(26):19803-7. [Content Brief]
[2]. Plowman GD, et al. Heregulin induces tyrosine phosphorylation of HER4/p180erbB4. Nature. 1993 Dec 2;366(6454):473-5. [Content Brief]
[3]. Ieguchi K, et al. Direct binding of the EGF-like domain of neuregulin-1 to integrins ({alpha}v{beta}3 and {alpha}6{beta}4) is involved in neuregulin-1/ErbB signaling. J Biol Chem. 2010 Oct 8;285(41):31388-98. [Content Brief]
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