Copy Link Print Information

C1GALT1 Antibody (YA9055)

(Synonyms: B3Gal T8, Beta 1,3 galactosyltransferase, C1GALT, Core 1 beta3 Gal T1, Core 1 O glycan T synthase)
Customer Review

Based on 1 Customer Validation

C1GALT1 Antibody (YA9055) is a Mouse-derived and non-conjugated IgG2a monoclonal antibody, targeting to C1GALT1.

For research use only. We do not sell to patients.
  • Host:

    Mouse

  • Isotype:

    IgG

  • Application:

    WB, ICC/IF, IF-Tissue, IHC-P, IP, ELISA

  • Reactivity :

    human

  • Formulation:

    Supplied in PBS(pH7.4) containing 0.1% gelatin and < 0.1% sodium azide.

  • Conjugation:
    Non-conjugated
  • Experimental Validation Results for C1GALT1 Antibody (YA9055)
  • C1GALT1 Antibody (YA9055) Product Image
Size
Price
Stock
Quantity
Amount: USD 0.00
Please select quantity
or
or

* Please select Quantity before adding items.

To place orders, for customer services and technical support, please contact: MedChemExpress USA

Tel: 609-228-6898 E-mail: [email protected] [email protected]

Service
Data Download
Related Products:
Top Publications Citing

Use of MCE Products

Applications

Application
WB Info
WB: Western Blot
ICC/IF Info
ICC/IF: Immunocytochemistry/
Immunofluorescence
IF-Tissue Info
IF-Tissue: Immunofluorescence-Tissue
IHC-P Info
IHC-P: Immunohistochemistry-Paraffin
IP Info
IP: Immunoprecipitation
ELISA Info
ELISA: Enzyme Linked Immunosorbent Assay
Dilution Ratio 1:500-1000 1:50-500 1:50-500 1:100-500 1-2μg per 100-500μg Total protein 1:50-3000

Product Details

Description

C1GALT1 Antibody (YA9055) is a Mouse-derived and non-conjugated IgG2a monoclonal antibody, targeting to C1GALT1.

  • Host Mouse
  • Species Reactivity
    human
  • Calculated Molecular Weight Predicted band size: 42 kDa
Species Reactivity Database

OMIM: 610555

Immunogen

A synthesized peptide derived from human C1GALT1.

Sensitivity

Endogenous

Purification

Affinity purified

Conjugation

Non-conjugated

Modification

Unmodified

Isotype

IgG

Product Properties

  • Appearance

    Solution

  • Formulation

    Supplied in PBS(pH7.4) containing 0.1% gelatin and < 0.1% sodium azide.

  • Concentration

    Batch-dependent, Please check the COA for the concentration of each lot. Check Lot Concentration

  • Storage & Stability

    Stored at 2-8°C for 1 year, do not freeze.

  • Shipping

    Shipping with blue ice.

Verification Images

  • Experimental Validation Results for C1GALT1 Antibody (YA9055)
    Western blot analysis was performed on protein extracts (25 μg) from HepG2 (lane 2), 293T (lane 3), HT-1080 (lane 4), and DU145 (lane 5) using C1GALT1 antibody. Proteins were transferred onto a 0.45 μm PVDF membrane using the Trans-Blot® Turbo™ system for 13 min. The membrane was then blocked with 5% nonfat milk in TBST (HY-K1025) for 1 h at room temperature. The primary antibody (1:1000) and loading control antibody GAPDH Antibody (HRP) (HY-P80954A) (1:5000) were diluted in 5% nonfat milk in TBST and incubated with the membrane overnight at 4°C. After washing, the membrane of primary antibody was incubated with HRP-conjugated goat anti-rabbit/mouse IgG secondary antibody (HY-P8001/HY-P8004) (1:5000) diluted in 5% nonfat milk in TBST for 1 h at room temperature. Protein bands were visualized using an Ultra High Sensitivity ECL detection kit (HY-K1005).

Background

  • Function

    C1GALT1 is a Glycosyltransferase that generates the core 1 O-glycan Gal-beta1-3GalNAc-alpha1-Ser/Thr (T antigen), which is a precursor for many extended O-glycans in glycoproteins. Plays a central role in many processes, such as angiogenesis, thrombopoiesis and kidney homeostasis development (By similarity)[1].

  • Subcellular Localization

    Membrane

  • Expression


    Tissue_Specificity: Widely expressed. Highly expressed in kidney, heart, placenta and liver.

  • Isoforms & Post-Translational Modification

    C1GALT1 has 2 isoforms, Q9NS00-1: amino acid length is 363, molecular weight is 42203 Da (predicted); Q9NS00-2: amino acid length is 309, molecular weight is 35935 Da (predicted).

  • Subunit

    Homodimer; disulfide-linked (By similarity).

  • SwissProt ID

    Q9NS00

  • Gene ID
    56913 [NCBI]
  • Synonyms

    B3Gal T8, Beta 1,3 galactosyltransferase, C1GALT, Core 1 beta3 Gal T1, Core 1 O glycan T synthase

References

[1]. Ju T, et al. Cloning and expression of human core 1 beta1,3-galactosyltransferase. J Biol Chem. 2002 Jan 4;277(1):178-86. [Content Brief]

C1GALT1 Antibody (YA9055) Related Classifications

MOQ
Minimum order quantity
100 mg

Get Quote In-stock

Other size
Get Quote
Please select quantity
Amount: USD 0.00

Measured by its ability to inhibit anti-CD3-induced proliferation of stimulated CTLL-2 mouse cytotoxic T cells. The ED50 this effect is 0.8023 μg/ml, corresponding to a specific activity is 1246.42 units/mg.

Assay Types
  • 1/16

    MedChemExpress Validation
     Western blot analysis was performed on protein extracts (25 μg) from HepG2 (lane 2), 293T (lane 3), HT-1080 (lane 4), and DU145 (lane 5) using C1GALT1 antibody. Proteins were transferred onto a 0.45 μm PVDF membrane using the Trans-Blot® Turbo™ system for 13 min. The membrane was then blocked with 5% nonfat milk in TBST (HY-K1025) for 1 h at room temperature. The primary antibody (1:1000) and loading control antibody GAPDH Antibody (HRP) (HY-P80954A) (1:5000) were diluted in 5% nonfat milk in TBST and incubated with the membrane overnight at 4°C. After washing, the membrane of primary antibody was incubated with HRP-conjugated goat anti-rabbit/mouse IgG secondary antibody (HY-P8001/HY-P8004) (1:5000) diluted in 5% nonfat milk in TBST for 1 h at room temperature. Protein bands were visualized using an Ultra High Sensitivity ECL detection kit (HY-K1005).

  • C1GALT1 Antibody (YA9055) Product Image
    1/16
  • Experimental Validation Results for C1GALT1 Antibody (YA9055)
  • C1GALT1 Antibody (YA9055) Product Image
  • Experimental Validation Results for C1GALT1 Antibody (YA9055)
    1/1
    MedChemExpress Validation
     Western blot analysis was performed on protein extracts (25 μg) from HepG2 (lane 2), 293T (lane 3), HT-1080 (lane 4), and DU145 (lane 5) using C1GALT1 antibody. Proteins were transferred onto a 0.45 μm PVDF membrane using the Trans-Blot® Turbo™ system for 13 min. The membrane was then blocked with 5% nonfat milk in TBST (HY-K1025) for 1 h at room temperature. The primary antibody (1:1000) and loading control antibody GAPDH Antibody (HRP) (HY-P80954A) (1:5000) were diluted in 5% nonfat milk in TBST and incubated with the membrane overnight at 4°C. After washing, the membrane of primary antibody was incubated with HRP-conjugated goat anti-rabbit/mouse IgG secondary antibody (HY-P8001/HY-P8004) (1:5000) diluted in 5% nonfat milk in TBST for 1 h at room temperature. Protein bands were visualized using an Ultra High Sensitivity ECL detection kit (HY-K1005).
  • Experimental Validation Results for C1GALT1 Antibody (YA9055)
  • C1GALT1 Antibody (YA9055) Product Image
    MedChemExpress Validation

    Western blot analysis was performed on protein extracts (25 μg) from HepG2 (lane 2), 293T (lane 3), HT-1080 (lane 4), and DU145 (lane 5) using C1GALT1 antibody. Proteins were transferred onto a 0.45 μm PVDF membrane using the Trans-Blot® Turbo™ system for 13 min. The membrane was then blocked with 5% nonfat milk in TBST (HY-K1025) for 1 h at room temperature. The primary antibody (1:1000) and loading control antibody GAPDH Antibody (HRP) (HY-P80954A) (1:5000) were diluted in 5% nonfat milk in TBST and incubated with the membrane overnight at 4°C. After washing, the membrane of primary antibody was incubated with HRP-conjugated goat anti-rabbit/mouse IgG secondary antibody (HY-P8001/HY-P8004) (1:5000) diluted in 5% nonfat milk in TBST for 1 h at room temperature. Protein bands were visualized using an Ultra High Sensitivity ECL detection kit (HY-K1005).

  • C1GALT1 Antibody (YA9055) Product Image WB