GNAI1 Antibody
(Synonyms: Guanine nucleotide-binding protein G(i) subunit alpha-1, Adenylate cyclase-inhibiting G alpha protein, GNAI1)GNAI1 Antibody is a Rabbit-derived and non-conjugated IgG Polyclonal antibody, targeting to GNAI1.
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Host:
Rabbit
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Isotype:
IgG
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Application:
WB, ICC/IF
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Reactivity :
Human, Mouse, Rat
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Formulation:
Supplied in PBS (pH 7.4), containing 30% glycerol, and 0.01% sodium azide.
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Conjugation:
Non-conjugated
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
Applications
| Application |
WB
WB: Western Blot
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ICC/IF
ICC/IF: Immunocytochemistry/
Immunofluorescence |
|---|---|---|
| Dilution Ratio | 1:1000-2000 | 1:50-200 |
Product Details
GNAI1 Antibody is a Rabbit-derived and non-conjugated IgG Polyclonal antibody, targeting to GNAI1.
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Host Rabbit
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Clonality Polyclonal
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Species ReactivityHuman, Mouse, Rat
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Observed Molecular WeightObserved band size: 40 kDa
Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.
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Calculated Molecular Weight Predicted band size: 40 kDa
Synthetic peptide corresponding to the center region of human GNAI1.
Endogenous
affinity purified.
Non-conjugated
Unmodified
IgG
Product Properties
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Appearance
Solution
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Formulation
Supplied in PBS (pH 7.4), containing 30% glycerol, and 0.01% sodium azide.
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Storage & Stability
Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.
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Shipping
Shipping with blue ice.
Background
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Function
GNAI1 is a Guanine nucleotide-binding proteins (G proteins) function as transducers downstream of G protein-coupled receptors (GPCRs) in numerous signaling cascades. The alpha chain contains the guanine nucleotide binding site and alternates between an active, GTP-bound state and an inactive, GDP-bound state. Signaling by an activated GPCR promotes GDP release and GTP binding. The alpha subunit has a low GTPase activity that converts bound GTP to GDP, thereby terminating the signal. Both GDP release and GTP hydrolysis are modulated by numerous regulatory proteins. Signaling is mediated via effector proteins, such as adenylate cyclase: inhibits adenylate cyclase activity of ADCY1, ADCY5 and ADCY6, leading to decreased intracellular cAMP levels. The inactive GDP-bound form prevents the association of RGS14 with centrosomes and is required for the translocation of RGS14 from the cytoplasm to the plasma membrane. Required for normal cytokinesis during mitosis. Required for cortical dynein-dynactin complex recruitment during metaphase[1][2][3][4][5][6][7][8][9][10][11][12][13][14].
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Subcellular Localization
Cell membrane; Nucleus; Cytoplasm; Cytoplasm, cytoskeleton, microtubule organizing center, centrosome; Cytoplasm, cell cortex
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Isoforms & Post-Translational Modification
GNAI1 has 2 isoforms, P63096-1: amino acid length is 354, molecular weight is 40361 Da (predicted); P63096-2: amino acid length is 302, molecular weight is 34775 Da (predicted).
Myristoylation at Gly-2 is required for membrane anchoring before palmitoylation. -
Subunit
Heterotrimeric G proteins are composed of 3 units; alpha, beta and gamma.
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SwissProt ID
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Synonyms
Guanine nucleotide-binding protein G(i) subunit alpha-1, Adenylate cyclase-inhibiting G alpha protein, GNAI1
Documentation
[1]. Soundararajan M, et al. Structural diversity in the RGS domain and its interaction with heterotrimeric G protein alpha-subunits. Proc Natl Acad Sci U S A. 2008 Apr 29;105(17):6457-62. [Content Brief]
[2]. Xu P, et al. Structural insights into the lipid and ligand regulation of serotonin receptors. Nature. 2021 Apr;592(7854):469-473. [Content Brief]
[3]. Huang S, et al. Structural basis for recognition of anti-migraine drug lasmiditan by the serotonin receptor 5-HT(1F)-G protein complex. Cell Res. 2021 Sep;31(9):1036-1038. [Content Brief]
[4]. Tan Y, et al. Structural insights into the ligand binding and G(i) coupling of serotonin receptor 5-HT(5A). Cell Discov. 2022 May 24;8(1):50. [Content Brief]
[5]. Xu Z, et al. Ligand recognition and G-protein coupling of trace amine receptor TAAR1. Nature. 2023 Dec;624(7992):672-681. [Content Brief]
[6]. Liu H, et al. Recognition of methamphetamine and other amines by trace amine receptor TAAR1. Nature. 2023 Dec;624(7992):663-671. [Content Brief]
[7]. Shang P, et al. Structural and signaling mechanisms of TAAR1 enabled preferential agonist design. Cell. 2023 Nov 22;186(24):5347-5362.e24. [Content Brief]
[8]. Chen Z, et al. Flexible scaffold-based cheminformatics approach for polypharmacological drug design. Cell. 2024 Apr 25;187(9):2194-2208.e22. [Content Brief]
[9]. Hunt TW, et al. RGS10 is a selective activator of G alpha i GTPase activity. Nature. 1996 Sep 12;383(6596):175-7. [Content Brief]
[10]. Zhang Z, et al. Molecular recognition of the atypical chemokine-like peptide GPR15L by its cognate receptor GPR15. Cell Discov. 2024 Jun 25;10(1):69. [Content Brief]
[11]. Lin H, et al. Metabolic signaling of ceramides through the FPR2 receptor inhibits adipocyte thermogenesis. Science. 2025 May;388(6746):eado4188. [Content Brief]
[12]. Taussig R, et al. Distinct patterns of bidirectional regulation of mammalian adenylyl cyclases. J Biol Chem. 1994 Feb 25;269(8):6093-100. [Content Brief]
[13]. Cho H, et al. Localization of Gi alpha proteins in the centrosomes and at the midbody: implication for their role in cell division. J Cell Biol. 2007 Jul 16;178(2):245-55. [Content Brief]
[14]. Kiyomitsu T, et al. Chromosome- and spindle-pole-derived signals generate an intrinsic code for spindle position and orientation. Nat Cell Biol. 2012 Feb 12;14(3):311-7. [Content Brief]
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